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P60840

- ENSA_MOUSE

UniProt

P60840 - ENSA_MOUSE

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Protein

Alpha-endosulfine

Gene

Ensa

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase. Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents (By similarity).By similarity

GO - Molecular functioni

  1. phosphatase inhibitor activity Source: UniProtKB
  2. potassium channel inhibitor activity Source: Ensembl
  3. protein phosphatase 2A binding Source: UniProtKB
  4. protein phosphatase inhibitor activity Source: UniProtKB-KW
  5. protein phosphatase type 2A regulator activity Source: UniProtKB

GO - Biological processi

  1. G2/M transition of mitotic cell cycle Source: UniProtKB
  2. mitotic nuclear division Source: UniProtKB
  3. negative regulation of catalytic activity Source: GOC
  4. regulation of catalytic activity Source: GOC
  5. regulation of insulin secretion Source: Ensembl
  6. response to glucose Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Protein phosphatase inhibitor

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiREACT_196577. MASTL Facilitates Mitotic Progression.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-endosulfine
Alternative name(s):
ARPP-19e
Gene namesi
Name:Ensa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:1891189. Ensa.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 121120Alpha-endosulfinePRO_0000146759Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21PhosphoserineBy similarity
Modified residuei67 – 671Phosphoserine; by GWLCurated
Modified residuei108 – 1081Phosphoserine; by PKABy similarity
Modified residuei109 – 1091Phosphoserine1 Publication

Post-translational modificationi

Phosphorylation at Ser-67 by GWL during mitosis is essential for interaction with PPP2R2D (PR55-delta) and subsequent inactivation of PP2A. Phosphorylated by PKA (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP60840.
PaxDbiP60840.
PRIDEiP60840.

PTM databases

PhosphoSiteiP60840.

Expressioni

Tissue specificityi

Present in striatum (at protein level).1 Publication

Gene expression databases

BgeeiP60840.
CleanExiMM_ENSA.
GenevestigatoriP60840.

Interactioni

Subunit structurei

Interacts (when phosphorylated at Ser-67) with PPP2R2D. Interacts with ABCC8. Interacts with SNCA; interaction is disrupted when phosphorylated at Ser-109 (By similarity).By similarity

Protein-protein interaction databases

IntActiP60840. 1 interaction.
MINTiMINT-4094447.

Family & Domainsi

Sequence similaritiesi

Belongs to the endosulfine family.Curated

Phylogenomic databases

eggNOGiNOG312450.
GeneTreeiENSGT00390000010139.
HOGENOMiHOG000232115.
HOVERGENiHBG000297.
InParanoidiP60840.
OMAiEQKYFDS.
OrthoDBiEOG7JT702.
PhylomeDBiP60840.
TreeFamiTF314718.

Family and domain databases

InterProiIPR006760. Endosulphine.
[Graphical view]
PfamiPF04667. Endosulfine. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P60840-1) [UniParc]FASTAAdd to Basket

Also known as: Alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQKQEEENP AEETGEEKQD TQEKEGILPE KAEEAKLKAK YPSLGQKPGG
60 70 80 90 100
SDFLMKRLQK GQKYFDSGDY NMAKAKMKNK QLPSAGADKN LVTGDHIPTP
110 120
QDLPQRKSSL VTSKLAGGQV E
Length:121
Mass (Da):13,335
Last modified:April 13, 2004 - v1
Checksum:iA84632C9085FFC5A
GO
Isoform 2 (identifier: P60840-2) [UniParc] [UniParc]FASTAAdd to Basket

Also known as: Beta

The sequence of this isoform differs from the canonical sequence as follows:
     118-121: Missing.

Show »
Length:117
Mass (Da):12,922
Checksum:iB85FFC5A907676C6
GO
Isoform 3 (identifier: P60840-3) [UniParc] [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     62-72: QKYFDSGDYNM → VWDTLILWHLG
     73-121: Missing.

Note: May be due to an intron retention.

Show »
Length:72
Mass (Da):8,183
Checksum:i6B020743FC2590B9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti24 – 241K → E in BAB27552. (PubMed:16141072)Curated
Sequence conflicti24 – 241K → E in BAB31584. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei62 – 7211QKYFDSGDYNM → VWDTLILWHLG in isoform 3. 1 PublicationVSP_037068Add
BLAST
Alternative sequencei73 – 12149Missing in isoform 3. 1 PublicationVSP_037069Add
BLAST
Alternative sequencei118 – 1214Missing in isoform 2. 2 PublicationsVSP_001444

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ005985 mRNA. Translation: CAA06799.1.
AK006149 mRNA. Translation: BAB24433.1.
AK011334 mRNA. Translation: BAB27552.1.
AK149726 mRNA. Translation: BAE29049.1.
AK161290 mRNA. Translation: BAE36298.1.
AK019170 mRNA. Translation: BAB31584.1.
CH466620 Genomic DNA. Translation: EDL38827.1.
BC058802 mRNA. Translation: AAH58802.1.
BC059724 mRNA. Translation: AAH59724.1.
CCDSiCCDS17617.1. [P60840-2]
CCDS50993.1.
RefSeqiNP_001021383.1. NM_001026212.1. [P60840-2]
NP_062507.1. NM_019561.2. [P60840-1]
UniGeneiMm.241365.

Genome annotation databases

EnsembliENSMUST00000037983; ENSMUSP00000045937; ENSMUSG00000038619. [P60840-2]
ENSMUST00000058230; ENSMUSP00000051799; ENSMUSG00000038619. [P60840-1]
GeneIDi56205.
KEGGimmu:56205.
UCSCiuc008qki.1. mouse. [P60840-3]
uc008qkj.1. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ005985 mRNA. Translation: CAA06799.1 .
AK006149 mRNA. Translation: BAB24433.1 .
AK011334 mRNA. Translation: BAB27552.1 .
AK149726 mRNA. Translation: BAE29049.1 .
AK161290 mRNA. Translation: BAE36298.1 .
AK019170 mRNA. Translation: BAB31584.1 .
CH466620 Genomic DNA. Translation: EDL38827.1 .
BC058802 mRNA. Translation: AAH58802.1 .
BC059724 mRNA. Translation: AAH59724.1 .
CCDSi CCDS17617.1. [P60840-2 ]
CCDS50993.1.
RefSeqi NP_001021383.1. NM_001026212.1. [P60840-2 ]
NP_062507.1. NM_019561.2. [P60840-1 ]
UniGenei Mm.241365.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P60840. 1 interaction.
MINTi MINT-4094447.

PTM databases

PhosphoSitei P60840.

Proteomic databases

MaxQBi P60840.
PaxDbi P60840.
PRIDEi P60840.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000037983 ; ENSMUSP00000045937 ; ENSMUSG00000038619 . [P60840-2 ]
ENSMUST00000058230 ; ENSMUSP00000051799 ; ENSMUSG00000038619 . [P60840-1 ]
GeneIDi 56205.
KEGGi mmu:56205.
UCSCi uc008qki.1. mouse. [P60840-3 ]
uc008qkj.1. mouse.

Organism-specific databases

CTDi 2029.
MGIi MGI:1891189. Ensa.

Phylogenomic databases

eggNOGi NOG312450.
GeneTreei ENSGT00390000010139.
HOGENOMi HOG000232115.
HOVERGENi HBG000297.
InParanoidi P60840.
OMAi EQKYFDS.
OrthoDBi EOG7JT702.
PhylomeDBi P60840.
TreeFami TF314718.

Enzyme and pathway databases

Reactomei REACT_196577. MASTL Facilitates Mitotic Progression.

Miscellaneous databases

ChiTaRSi ENSA. mouse.
NextBioi 312033.
PROi P60840.
SOURCEi Search...

Gene expression databases

Bgeei P60840.
CleanExi MM_ENSA.
Genevestigatori P60840.

Family and domain databases

InterProi IPR006760. Endosulphine.
[Graphical view ]
Pfami PF04667. Endosulfine. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Human alpha-endosulfine, a possible regulator of sulfonylurea-sensitive K(ATP) channel: molecular cloning, expression and biological properties."
    Heron L., Virsolvy A., Peyrollier K., Gribble F.M., Le Cam A., Ashcroft F.M., Bataille D.
    Proc. Natl. Acad. Sci. U.S.A. 95:8387-8391(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Bone marrow, Embryo and Testis.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
    Strain: FVB/N.
    Tissue: Colon and Testis.
  5. "ARPP-16/ARPP-19: a highly conserved family of cAMP-regulated phosphoproteins."
    Dulubova I., Horiuchi A., Snyder G.L., Girault J.-A., Czernik A.J., Shao L., Ramabhadran R., Greengard P., Nairn A.C.
    J. Neurochem. 77:229-238(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, PHOSPHORYLATION AT SER-109.

Entry informationi

Entry nameiENSA_MOUSE
AccessioniPrimary (citable) accession number: P60840
Secondary accession number(s): Q5D069
, Q6PBG4, Q9CQZ9, Q9Z0G1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: October 29, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3