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P60840

- ENSA_MOUSE

UniProt

P60840 - ENSA_MOUSE

Protein

Alpha-endosulfine

Gene

Ensa

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase. Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents By similarity.By similarity

    GO - Molecular functioni

    1. phosphatase inhibitor activity Source: UniProtKB
    2. protein phosphatase 2A binding Source: UniProtKB
    3. protein phosphatase inhibitor activity Source: UniProtKB-KW
    4. protein phosphatase type 2A regulator activity Source: UniProtKB

    GO - Biological processi

    1. G2/M transition of mitotic cell cycle Source: UniProtKB
    2. mitotic nuclear division Source: UniProtKB
    3. negative regulation of catalytic activity Source: GOC
    4. regulation of catalytic activity Source: GOC
    5. response to glucose Source: Ensembl

    Keywords - Molecular functioni

    Protein phosphatase inhibitor

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    ReactomeiREACT_196577. MASTL Facilitates Mitotic Progression.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-endosulfine
    Alternative name(s):
    ARPP-19e
    Gene namesi
    Name:Ensa
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:1891189. Ensa.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 121120Alpha-endosulfinePRO_0000146759Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei2 – 21PhosphoserineBy similarity
    Modified residuei67 – 671Phosphoserine; by GWLCurated
    Modified residuei108 – 1081Phosphoserine; by PKABy similarity
    Modified residuei109 – 1091Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylation at Ser-67 by GWL during mitosis is essential for interaction with PPP2R2D (PR55-delta) and subsequent inactivation of PP2A. Phosphorylated by PKA By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP60840.
    PaxDbiP60840.
    PRIDEiP60840.

    PTM databases

    PhosphoSiteiP60840.

    Expressioni

    Tissue specificityi

    Present in striatum (at protein level).1 Publication

    Gene expression databases

    BgeeiP60840.
    CleanExiMM_ENSA.
    GenevestigatoriP60840.

    Interactioni

    Subunit structurei

    Interacts (when phosphorylated at Ser-67) with PPP2R2D. Interacts with ABCC8. Interacts with SNCA; interaction is disrupted when phosphorylated at Ser-109 By similarity.By similarity

    Protein-protein interaction databases

    IntActiP60840. 1 interaction.
    MINTiMINT-4094447.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the endosulfine family.Curated

    Phylogenomic databases

    eggNOGiNOG312450.
    GeneTreeiENSGT00390000010139.
    HOGENOMiHOG000232115.
    HOVERGENiHBG000297.
    InParanoidiQ5D069.
    OMAiEQKYFDS.
    OrthoDBiEOG7JT702.
    PhylomeDBiP60840.
    TreeFamiTF314718.

    Family and domain databases

    InterProiIPR006760. Endosulphine.
    [Graphical view]
    PfamiPF04667. Endosulfine. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P60840-1) [UniParc]FASTAAdd to Basket

    Also known as: Alpha

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSQKQEEENP AEETGEEKQD TQEKEGILPE KAEEAKLKAK YPSLGQKPGG    50
    SDFLMKRLQK GQKYFDSGDY NMAKAKMKNK QLPSAGADKN LVTGDHIPTP 100
    QDLPQRKSSL VTSKLAGGQV E 121
    Length:121
    Mass (Da):13,335
    Last modified:April 13, 2004 - v1
    Checksum:iA84632C9085FFC5A
    GO
    Isoform 2 (identifier: P60840-2) [UniParc] [UniParc]FASTAAdd to Basket

    Also known as: Beta

    The sequence of this isoform differs from the canonical sequence as follows:
         118-121: Missing.

    Show »
    Length:117
    Mass (Da):12,922
    Checksum:iB85FFC5A907676C6
    GO
    Isoform 3 (identifier: P60840-3) [UniParc] [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         62-72: QKYFDSGDYNM → VWDTLILWHLG
         73-121: Missing.

    Note: May be due to an intron retention.

    Show »
    Length:72
    Mass (Da):8,183
    Checksum:i6B020743FC2590B9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti24 – 241K → E in BAB27552. (PubMed:16141072)Curated
    Sequence conflicti24 – 241K → E in BAB31584. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei62 – 7211QKYFDSGDYNM → VWDTLILWHLG in isoform 3. 1 PublicationVSP_037068Add
    BLAST
    Alternative sequencei73 – 12149Missing in isoform 3. 1 PublicationVSP_037069Add
    BLAST
    Alternative sequencei118 – 1214Missing in isoform 2. 2 PublicationsVSP_001444

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ005985 mRNA. Translation: CAA06799.1.
    AK006149 mRNA. Translation: BAB24433.1.
    AK011334 mRNA. Translation: BAB27552.1.
    AK149726 mRNA. Translation: BAE29049.1.
    AK161290 mRNA. Translation: BAE36298.1.
    AK019170 mRNA. Translation: BAB31584.1.
    CH466620 Genomic DNA. Translation: EDL38827.1.
    BC058802 mRNA. Translation: AAH58802.1.
    BC059724 mRNA. Translation: AAH59724.1.
    CCDSiCCDS17617.1. [P60840-2]
    CCDS50993.1.
    RefSeqiNP_001021383.1. NM_001026212.1. [P60840-2]
    NP_062507.1. NM_019561.2. [P60840-1]
    UniGeneiMm.241365.

    Genome annotation databases

    EnsembliENSMUST00000037983; ENSMUSP00000045937; ENSMUSG00000038619. [P60840-2]
    ENSMUST00000058230; ENSMUSP00000051799; ENSMUSG00000038619. [P60840-1]
    GeneIDi56205.
    KEGGimmu:56205.
    UCSCiuc008qki.1. mouse. [P60840-3]
    uc008qkj.1. mouse.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ005985 mRNA. Translation: CAA06799.1 .
    AK006149 mRNA. Translation: BAB24433.1 .
    AK011334 mRNA. Translation: BAB27552.1 .
    AK149726 mRNA. Translation: BAE29049.1 .
    AK161290 mRNA. Translation: BAE36298.1 .
    AK019170 mRNA. Translation: BAB31584.1 .
    CH466620 Genomic DNA. Translation: EDL38827.1 .
    BC058802 mRNA. Translation: AAH58802.1 .
    BC059724 mRNA. Translation: AAH59724.1 .
    CCDSi CCDS17617.1. [P60840-2 ]
    CCDS50993.1.
    RefSeqi NP_001021383.1. NM_001026212.1. [P60840-2 ]
    NP_062507.1. NM_019561.2. [P60840-1 ]
    UniGenei Mm.241365.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P60840. 1 interaction.
    MINTi MINT-4094447.

    PTM databases

    PhosphoSitei P60840.

    Proteomic databases

    MaxQBi P60840.
    PaxDbi P60840.
    PRIDEi P60840.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000037983 ; ENSMUSP00000045937 ; ENSMUSG00000038619 . [P60840-2 ]
    ENSMUST00000058230 ; ENSMUSP00000051799 ; ENSMUSG00000038619 . [P60840-1 ]
    GeneIDi 56205.
    KEGGi mmu:56205.
    UCSCi uc008qki.1. mouse. [P60840-3 ]
    uc008qkj.1. mouse.

    Organism-specific databases

    CTDi 2029.
    MGIi MGI:1891189. Ensa.

    Phylogenomic databases

    eggNOGi NOG312450.
    GeneTreei ENSGT00390000010139.
    HOGENOMi HOG000232115.
    HOVERGENi HBG000297.
    InParanoidi Q5D069.
    OMAi EQKYFDS.
    OrthoDBi EOG7JT702.
    PhylomeDBi P60840.
    TreeFami TF314718.

    Enzyme and pathway databases

    Reactomei REACT_196577. MASTL Facilitates Mitotic Progression.

    Miscellaneous databases

    ChiTaRSi ENSA. mouse.
    NextBioi 312033.
    PROi P60840.
    SOURCEi Search...

    Gene expression databases

    Bgeei P60840.
    CleanExi MM_ENSA.
    Genevestigatori P60840.

    Family and domain databases

    InterProi IPR006760. Endosulphine.
    [Graphical view ]
    Pfami PF04667. Endosulfine. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Human alpha-endosulfine, a possible regulator of sulfonylurea-sensitive K(ATP) channel: molecular cloning, expression and biological properties."
      Heron L., Virsolvy A., Peyrollier K., Gribble F.M., Le Cam A., Ashcroft F.M., Bataille D.
      Proc. Natl. Acad. Sci. U.S.A. 95:8387-8391(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Brain.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Bone marrow, Embryo and Testis.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
      Strain: FVB/N.
      Tissue: Colon and Testis.
    5. "ARPP-16/ARPP-19: a highly conserved family of cAMP-regulated phosphoproteins."
      Dulubova I., Horiuchi A., Snyder G.L., Girault J.-A., Czernik A.J., Shao L., Ramabhadran R., Greengard P., Nairn A.C.
      J. Neurochem. 77:229-238(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, PHOSPHORYLATION AT SER-109.

    Entry informationi

    Entry nameiENSA_MOUSE
    AccessioniPrimary (citable) accession number: P60840
    Secondary accession number(s): Q5D069
    , Q6PBG4, Q9CQZ9, Q9Z0G1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: April 13, 2004
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3