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P60840 (ENSA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-endosulfine
Alternative name(s):
ARPP-19e
Gene names
Name:Ensa
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length121 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase. Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents By similarity.

Subunit structure

Interacts (when phosphorylated at Ser-67) with PPP2R2D. Interacts with ABCC8. Interacts with SNCA; interaction is disrupted when phosphorylated at Ser-109 By similarity.

Subcellular location

Cytoplasm By similarity.

Tissue specificity

Present in striatum (at protein level). Ref.5

Post-translational modification

Phosphorylation at Ser-67 by GWL during mitosis is essential for interaction with PPP2R2D (PR55-delta) and subsequent inactivation of PP2A. Phosphorylated by PKA By similarity. Ref.5

Sequence similarities

Belongs to the endosulfine family.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P60840-1)

Also known as: Alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P60840-2)

Also known as: Beta;

The sequence of this isoform differs from the canonical sequence as follows:
     118-121: Missing.
Isoform 3 (identifier: P60840-3)

The sequence of this isoform differs from the canonical sequence as follows:
     62-72: QKYFDSGDYNM → VWDTLILWHLG
     73-121: Missing.
Note: May be due to an intron retention.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 121120Alpha-endosulfine
PRO_0000146759

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue21Phosphoserine By similarity
Modified residue671Phosphoserine; by GWL Probable
Modified residue1081Phosphoserine; by PKA By similarity
Modified residue1091Phosphoserine Ref.5

Natural variations

Alternative sequence62 – 7211QKYFDSGDYNM → VWDTLILWHLG in isoform 3.
VSP_037068
Alternative sequence73 – 12149Missing in isoform 3.
VSP_037069
Alternative sequence118 – 1214Missing in isoform 2.
VSP_001444

Experimental info

Sequence conflict241K → E in BAB27552. Ref.2
Sequence conflict241K → E in BAB31584. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Alpha) [UniParc].

Last modified April 13, 2004. Version 1.
Checksum: A84632C9085FFC5A

FASTA12113,335
        10         20         30         40         50         60 
MSQKQEEENP AEETGEEKQD TQEKEGILPE KAEEAKLKAK YPSLGQKPGG SDFLMKRLQK 

        70         80         90        100        110        120 
GQKYFDSGDY NMAKAKMKNK QLPSAGADKN LVTGDHIPTP QDLPQRKSSL VTSKLAGGQV 


E 

« Hide

Isoform 2 (Beta) [UniParc] [UniParc].

Checksum: B85FFC5A907676C6
Show »

FASTA11712,922
Isoform 3 [UniParc] [UniParc].

Checksum: 6B020743FC2590B9
Show »

FASTA728,183

References

« Hide 'large scale' references
[1]"Human alpha-endosulfine, a possible regulator of sulfonylurea-sensitive K(ATP) channel: molecular cloning, expression and biological properties."
Heron L., Virsolvy A., Peyrollier K., Gribble F.M., Le Cam A., Ashcroft F.M., Bataille D.
Proc. Natl. Acad. Sci. U.S.A. 95:8387-8391(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Bone marrow, Embryo and Testis.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Strain: FVB/N.
Tissue: Colon and Testis.
[5]"ARPP-16/ARPP-19: a highly conserved family of cAMP-regulated phosphoproteins."
Dulubova I., Horiuchi A., Snyder G.L., Girault J.-A., Czernik A.J., Shao L., Ramabhadran R., Greengard P., Nairn A.C.
J. Neurochem. 77:229-238(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, PHOSPHORYLATION AT SER-109.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ005985 mRNA. Translation: CAA06799.1.
AK006149 mRNA. Translation: BAB24433.1.
AK011334 mRNA. Translation: BAB27552.1.
AK149726 mRNA. Translation: BAE29049.1.
AK161290 mRNA. Translation: BAE36298.1.
AK019170 mRNA. Translation: BAB31584.1.
CH466620 Genomic DNA. Translation: EDL38827.1.
BC058802 mRNA. Translation: AAH58802.1.
BC059724 mRNA. Translation: AAH59724.1.
CCDSCCDS17617.1. [P60840-2]
CCDS50993.1.
RefSeqNP_001021383.1. NM_001026212.1. [P60840-2]
NP_062507.1. NM_019561.2. [P60840-1]
UniGeneMm.241365.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP60840. 1 interaction.
MINTMINT-4094447.

PTM databases

PhosphoSiteP60840.

Proteomic databases

MaxQBP60840.
PaxDbP60840.
PRIDEP60840.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000037983; ENSMUSP00000045937; ENSMUSG00000038619. [P60840-2]
ENSMUST00000058230; ENSMUSP00000051799; ENSMUSG00000038619. [P60840-1]
GeneID56205.
KEGGmmu:56205.
UCSCuc008qki.1. mouse. [P60840-3]
uc008qkj.1. mouse.

Organism-specific databases

CTD2029.
MGIMGI:1891189. Ensa.

Phylogenomic databases

eggNOGNOG312450.
GeneTreeENSGT00390000010139.
HOGENOMHOG000232115.
HOVERGENHBG000297.
InParanoidQ5D069.
OMAEQKYFDS.
OrthoDBEOG7JT702.
PhylomeDBP60840.
TreeFamTF314718.

Gene expression databases

BgeeP60840.
CleanExMM_ENSA.
GenevestigatorP60840.

Family and domain databases

InterProIPR006760. Endosulphine.
[Graphical view]
PfamPF04667. Endosulfine. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSENSA. mouse.
NextBio312033.
PROP60840.
SOURCESearch...

Entry information

Entry nameENSA_MOUSE
AccessionPrimary (citable) accession number: P60840
Secondary accession number(s): Q5D069 expand/collapse secondary AC list , Q6PBG4, Q9CQZ9, Q9Z0G1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: July 9, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot