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Protein

Ribonuclease P protein component 1

Gene

rnp1

Organism
Methanococcus maripaludis (strain S2 / LL)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.UniRule annotation1 Publication

Catalytic activityi

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.UniRule annotation

Kineticsi

kcat 10 min(-1) in absence of L7Ae, 63 min(-1) in presence of L7Ae. Kinetic parameters determined at 37 degrees Celsius.

  1. KM=2.6 µM for pre-tRNA-Tyr in the absence of L7Ae1 Publication
  2. KM=0.044 µM for pre-tRNA-Tyr in the presence of L7Ae1 Publication

    Temperature dependencei

    Optimum temperature is 36-38 degrees Celsius in the absence of L7Ae, 48-50 degrees Celsius in presence of L7Ae.1 Publication

    GO - Molecular functioni

    • ribonuclease P activity Source: UniProtKB
    • RNA binding Source: InterPro

    GO - Biological processi

    • mRNA cleavage Source: InterPro
    • RNA phosphodiester bond hydrolysis Source: GOC
    • RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
    • rRNA processing Source: InterPro
    • tRNA 5'-leader removal Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    tRNA processing

    Enzyme and pathway databases

    BioCyciMMAR267377:GJ77-1444-MONOMER.
    BRENDAi3.1.26.5. 3262.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease P protein component 1UniRule annotation (EC:3.1.26.5UniRule annotation)
    Short name:
    RNase P component 1UniRule annotation
    Alternative name(s):
    Rpp29UniRule annotation
    Gene namesi
    Name:rnp1UniRule annotation
    Ordered Locus Names:MMP1407
    OrganismiMethanococcus maripaludis (strain S2 / LL)
    Taxonomic identifieri267377 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
    ProteomesiUP000000590 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: UniProtKB
    • ribonuclease MRP complex Source: InterPro
    • ribonuclease P complex Source: UniProtKB
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 9696Ribonuclease P protein component 1PRO_0000128432Add
    BLAST

    Interactioni

    Subunit structurei

    Consists of a catalytic RNA component and at least 5 protein subunits.1 Publication

    Protein-protein interaction databases

    STRINGi267377.MMP1407.

    Structurei

    3D structure databases

    ProteinModelPortaliP60832.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the eukaryotic/archaeal RNase P protein component 1 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1588.
    HOGENOMiHOG000231353.
    KOiK03538.
    OMAiWHELIGL.

    Family and domain databases

    Gene3Di2.30.30.210. 1 hit.
    HAMAPiMF_00754. RNase_P_1.
    InterProiIPR002730. RNase_P/MRP_p29.
    IPR023538. RNP1.
    IPR023534. Rof/RNase_P-like.
    [Graphical view]
    PfamiPF01868. UPF0086. 1 hit.
    [Graphical view]
    SUPFAMiSSF101744. SSF101744. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P60832-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSQNILRHEL IGLNLEVVKS TDSGLISTKG RVINETRNTL VLEKENGKEI
    60 70 80 90
    TLVKEISTFR IQFESENVVK IDIDGRLLVG RPEDRLKRKI KQLYSY
    Length:96
    Mass (Da):11,069
    Last modified:April 13, 2004 - v1
    Checksum:i0319FE7AC4C64879
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BX950229 Genomic DNA. Translation: CAF30963.1.
    RefSeqiNP_988527.1. NC_005791.1.
    WP_011171351.1. NC_005791.1.

    Genome annotation databases

    EnsemblBacteriaiCAF30963; CAF30963; MMP1407.
    GeneIDi2762428.
    KEGGimmp:MMP1407.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BX950229 Genomic DNA. Translation: CAF30963.1.
    RefSeqiNP_988527.1. NC_005791.1.
    WP_011171351.1. NC_005791.1.

    3D structure databases

    ProteinModelPortaliP60832.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi267377.MMP1407.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAF30963; CAF30963; MMP1407.
    GeneIDi2762428.
    KEGGimmp:MMP1407.

    Phylogenomic databases

    eggNOGiCOG1588.
    HOGENOMiHOG000231353.
    KOiK03538.
    OMAiWHELIGL.

    Enzyme and pathway databases

    BioCyciMMAR267377:GJ77-1444-MONOMER.
    BRENDAi3.1.26.5. 3262.

    Family and domain databases

    Gene3Di2.30.30.210. 1 hit.
    HAMAPiMF_00754. RNase_P_1.
    InterProiIPR002730. RNase_P/MRP_p29.
    IPR023538. RNP1.
    IPR023534. Rof/RNase_P-like.
    [Graphical view]
    PfamiPF01868. UPF0086. 1 hit.
    [Graphical view]
    SUPFAMiSSF101744. SSF101744. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: S2 / LL.
    2. Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION.
      Strain: S2 / LL.

    Entry informationi

    Entry nameiRNP1_METMP
    AccessioniPrimary (citable) accession number: P60832
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: April 13, 2004
    Last modified: April 29, 2015
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.