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Protein

Cold-inducible RNA-binding protein

Gene

Cirbp

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor. Promotes assembly of stress granules (SGs), when overexpressed (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Stress response

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cold-inducible RNA-binding protein
Alternative name(s):
A18 hnRNP
Glycine-rich RNA-binding protein CIRP
Gene namesi
Name:Cirbp
Synonyms:Cirp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi620756. Cirbp.

Subcellular locationi

  • Nucleusnucleoplasm
  • Cytoplasm

  • Note: Translocates from the nucleus to the cytoplasm after exposure to UV radiation. Translocates from the nucleus to the cytoplasm into stress granules upon various cytoplasmic stresses, such as osmotic and heat shocks. Its recruitment into stress granules occurs in the absence of TIAR proteins (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 172172Cold-inducible RNA-binding proteinPRO_0000081506Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei130 – 1301PhosphoserineBy similarity
Modified residuei138 – 1381PhosphoserineBy similarity
Modified residuei146 – 1461PhosphoserineBy similarity
Modified residuei156 – 1561PhosphoserineBy similarity
Modified residuei159 – 1591PhosphoserineBy similarity
Modified residuei163 – 1631PhosphoserineBy similarity

Post-translational modificationi

Methylated on arginine residues. Methylation of the RGG motifs is a prerequisite for recruitment into SGs (By similarity).By similarity
Phosphorylated by CK2, GSK3A and GSK3B. Phosphorylation by GSK3B increases RNA-binding activity to the TXN 3'-UTR transcript upon exposure to UV radiation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP60825.
PRIDEiP60825.

Expressioni

Inductioni

By cold stress.

Gene expression databases

BgeeiENSRNOG00000015999.
GenevisibleiP60825. RN.

Interactioni

Subunit structurei

Interacts with EIF4G1. Associates with ribosomes (By similarity).By similarity

Protein-protein interaction databases

IntActiP60825. 1 interaction.
STRINGi10116.ENSRNOP00000021648.

Structurei

3D structure databases

ProteinModelPortaliP60825.
SMRiP60825. Positions 1-90.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 8479RRMPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi92 – 15867Gly-richAdd
BLAST

Domaini

Both the RRM domain and the arginine, glycine (RGG) rich domain are necessary for binding to the TXN 3'-untranslated region. Both the RRM domain and the arginine, glycine (RGG) rich domain (RGG repeats) are necessary for optimal recruitment into SGs upon cellular stress. The C-terminal domain containing RGG repeats is necessary for translational repression (By similarity).By similarity

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0118. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00710000106295.
HOGENOMiHOG000276232.
HOVERGENiHBG107480.
InParanoidiP60825.
OMAiYNSRSQG.
OrthoDBiEOG091G13K0.
PhylomeDBiP60825.
TreeFamiTF354331.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00361. RRM_1. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P60825-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASDEGKLFV GGLSFDTNEQ ALEQVFSKYG QISEVVVVKD RETQRSRGFG
60 70 80 90 100
FVTFENIDDA KDAMMAMNGK SVDGRQIRVD QAGKSSDNRS RGYRGGSAGG
110 120 130 140 150
RGFFRGGRSR GRGFSRGGGD RGYGGGRFES RSGGYGGSRD YYASRSQGGS
160 170
YGYRSSGGSY RDSYDSYATH NE
Length:172
Mass (Da):18,607
Last modified:April 13, 2004 - v1
Checksum:iDA0E53088FBED4C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000362 mRNA. Translation: BAA19092.1.
RefSeqiXP_006241076.1. XM_006241014.2.
UniGeneiRn.28931.

Genome annotation databases

EnsembliENSRNOT00000021648; ENSRNOP00000021648; ENSRNOG00000015999.
GeneIDi81825.
UCSCiRGD:620756. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000362 mRNA. Translation: BAA19092.1.
RefSeqiXP_006241076.1. XM_006241014.2.
UniGeneiRn.28931.

3D structure databases

ProteinModelPortaliP60825.
SMRiP60825. Positions 1-90.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP60825. 1 interaction.
STRINGi10116.ENSRNOP00000021648.

Proteomic databases

PaxDbiP60825.
PRIDEiP60825.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000021648; ENSRNOP00000021648; ENSRNOG00000015999.
GeneIDi81825.
UCSCiRGD:620756. rat.

Organism-specific databases

CTDi1153.
RGDi620756. Cirbp.

Phylogenomic databases

eggNOGiKOG0118. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00710000106295.
HOGENOMiHOG000276232.
HOVERGENiHBG107480.
InParanoidiP60825.
OMAiYNSRSQG.
OrthoDBiEOG091G13K0.
PhylomeDBiP60825.
TreeFamiTF354331.

Miscellaneous databases

PROiP60825.

Gene expression databases

BgeeiENSRNOG00000015999.
GenevisibleiP60825. RN.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00361. RRM_1. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCIRBP_RAT
AccessioniPrimary (citable) accession number: P60825
Secondary accession number(s): O09069, O09148, Q61413
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: September 7, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.