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Protein

Elongation factor 4

Gene

lepA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.UniRule annotation2 Publications

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 196GTPUniRule annotation
Nucleotide bindingi131 – 1344GTPUniRule annotation

GO - Molecular functioni

  • GTPase activity Source: EcoCyc
  • GTP binding Source: UniProtKB-HAMAP
  • ribosomal large subunit binding Source: EcoCyc
  • ribosomal small subunit binding Source: EcoCyc
  • ribosome binding Source: UniProtKB-HAMAP
  • translation elongation factor activity Source: EcoCyc

GO - Biological processi

  • positive regulation of translation Source: UniProtKB-HAMAP
  • response to cold Source: EcoCyc
  • response to pH Source: EcoCyc
  • response to salt stress Source: EcoCyc
  • translational elongation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10529-MONOMER.
ECOL316407:JW2553-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 4UniRule annotation (EC:3.6.5.n1UniRule annotation)
Short name:
EF-4UniRule annotation
Alternative name(s):
Ribosomal back-translocase LepAUniRule annotation
Gene namesi
Name:lepAUniRule annotation
Ordered Locus Names:b2569, JW2553
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10529. lepA.

Subcellular locationi

  • Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 599599Elongation factor 4PRO_0000176271Add
BLAST

Proteomic databases

EPDiP60785.
PaxDbiP60785.
PRIDEiP60785.

Interactioni

Protein-protein interaction databases

BioGridi4260596. 215 interactions.
DIPiDIP-29868N.
IntActiP60785. 22 interactions.
MINTiMINT-1225739.
STRINGi511145.b2569.

Structurei

Secondary structure

1
599
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 107Combined sources
Helixi20 – 278Combined sources
Beta strandi58 – 647Combined sources
Beta strandi70 – 778Combined sources
Helixi82 – 843Combined sources
Helixi85 – 9410Combined sources
Beta strandi96 – 1038Combined sources
Turni104 – 1063Combined sources
Helixi111 – 12111Combined sources
Beta strandi125 – 1317Combined sources
Helixi140 – 14910Combined sources
Beta strandi159 – 1613Combined sources
Turni163 – 1653Combined sources
Helixi169 – 17911Combined sources
Beta strandi192 – 20110Combined sources
Turni202 – 2043Combined sources
Beta strandi205 – 21511Combined sources
Beta strandi217 – 2204Combined sources
Beta strandi222 – 2254Combined sources
Turni226 – 2283Combined sources
Beta strandi231 – 2333Combined sources
Beta strandi236 – 24611Combined sources
Beta strandi248 – 2503Combined sources
Beta strandi255 – 2595Combined sources
Helixi265 – 2673Combined sources
Beta strandi273 – 2797Combined sources
Beta strandi295 – 3039Combined sources
Helixi304 – 3063Combined sources
Helixi307 – 31812Combined sources
Beta strandi325 – 3317Combined sources
Turni332 – 3343Combined sources
Beta strandi335 – 34410Combined sources
Helixi345 – 35713Combined sources
Beta strandi363 – 3653Combined sources
Beta strandi372 – 3798Combined sources
Beta strandi381 – 3866Combined sources
Helixi387 – 3893Combined sources
Helixi393 – 3953Combined sources
Beta strandi396 – 41116Combined sources
Helixi412 – 4143Combined sources
Helixi415 – 42410Combined sources
Beta strandi428 – 4336Combined sources
Beta strandi438 – 4469Combined sources
Helixi447 – 4515Combined sources
Turni452 – 4543Combined sources
Helixi455 – 4617Combined sources
Beta strandi467 – 47711Combined sources
Beta strandi480 – 4878Combined sources
Beta strandi490 – 50011Combined sources
Helixi501 – 5033Combined sources
Helixi504 – 51815Combined sources
Beta strandi526 – 5327Combined sources
Beta strandi535 – 5428Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CB4X-ray2.80A/B/C/D/E/F1-599[»]
3DEGelectron microscopy-C1-545[»]
3JCDelectron microscopy3.70x1-599[»]
3JCEelectron microscopy3.20x1-599[»]
ProteinModelPortaliP60785.
SMRiP60785. Positions 1-555.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60785.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 184183tr-type GAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4S. Bacteria.
COG0481. LUCA.
HOGENOMiHOG000020624.
InParanoidiP60785.
KOiK03596.
OMAiFAGIYPV.
PhylomeDBiP60785.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00071. LepA. 1 hit.
InterProiIPR006297. EF-4.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P60785-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNIRNFSII AHIDHGKSTL SDRIIQICGG LSDREMEAQV LDSMDLERER
60 70 80 90 100
GITIKAQSVT LDYKASDGET YQLNFIDTPG HVDFSYEVSR SLAACEGALL
110 120 130 140 150
VVDAGQGVEA QTLANCYTAM EMDLEVVPVL NKIDLPAADP ERVAEEIEDI
160 170 180 190 200
VGIDATDAVR CSAKTGVGVQ DVLERLVRDI PPPEGDPEGP LQALIIDSWF
210 220 230 240 250
DNYLGVVSLI RIKNGTLRKG DKVKVMSTGQ TYNADRLGIF TPKQVDRTEL
260 270 280 290 300
KCGEVGWLVC AIKDIHGAPV GDTLTLARNP AEKALPGFKK VKPQVYAGLF
310 320 330 340 350
PVSSDDYEAF RDALGKLSLN DASLFYEPES SSALGFGFRC GFLGLLHMEI
360 370 380 390 400
IQERLEREYD LDLITTAPTV VYEVETTSRE VIYVDSPSKL PAVNNIYELR
410 420 430 440 450
EPIAECHMLL PQAYLGNVIT LCVEKRGVQT NMVYHGNQVA LTYEIPMAEV
460 470 480 490 500
VLDFFDRLKS TSRGYASLDY NFKRFQASDM VRVDVLINGE RVDALALITH
510 520 530 540 550
RDNSQNRGRE LVEKMKDLIP RQQFDIAIQA AIGTHIIARS TVKQLRKNVL
560 570 580 590
AKCYGGDISR KKKLLQKQKE GKKRMKQIGN VELPQEAFLA ILHVGKDNK
Length:599
Mass (Da):66,570
Last modified:April 13, 2004 - v1
Checksum:i91E3678557A46D49
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti156 – 18530TDAVR…PPPEG → HRRGALFSENRRWCAGRSRT SGARHSAAGS (PubMed:2987248).CuratedAdd
BLAST
Sequence conflicti156 – 18530TDAVR…PPPEG → HRRGALFSENRRWCAGRSRT SGARHSAAGS (Ref. 2) CuratedAdd
BLAST
Sequence conflicti266 – 2661Missing (PubMed:2987248).Curated
Sequence conflicti266 – 2661Missing (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00426 Genomic DNA. Translation: AAA24063.1.
D64044 Genomic DNA. Translation: BAA10916.1.
U00096 Genomic DNA. Translation: AAC75622.1.
AP009048 Genomic DNA. Translation: BAE76745.1.
PIRiH65034. BVECLA.
RefSeqiNP_417064.1. NC_000913.3.
WP_000790168.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75622; AAC75622; b2569.
BAE76745; BAE76745; BAE76745.
GeneIDi947051.
KEGGiecj:JW2553.
eco:b2569.
PATRICi32120537. VBIEscCol129921_2671.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00426 Genomic DNA. Translation: AAA24063.1.
D64044 Genomic DNA. Translation: BAA10916.1.
U00096 Genomic DNA. Translation: AAC75622.1.
AP009048 Genomic DNA. Translation: BAE76745.1.
PIRiH65034. BVECLA.
RefSeqiNP_417064.1. NC_000913.3.
WP_000790168.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CB4X-ray2.80A/B/C/D/E/F1-599[»]
3DEGelectron microscopy-C1-545[»]
3JCDelectron microscopy3.70x1-599[»]
3JCEelectron microscopy3.20x1-599[»]
ProteinModelPortaliP60785.
SMRiP60785. Positions 1-555.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260596. 215 interactions.
DIPiDIP-29868N.
IntActiP60785. 22 interactions.
MINTiMINT-1225739.
STRINGi511145.b2569.

Proteomic databases

EPDiP60785.
PaxDbiP60785.
PRIDEiP60785.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75622; AAC75622; b2569.
BAE76745; BAE76745; BAE76745.
GeneIDi947051.
KEGGiecj:JW2553.
eco:b2569.
PATRICi32120537. VBIEscCol129921_2671.

Organism-specific databases

EchoBASEiEB0524.
EcoGeneiEG10529. lepA.

Phylogenomic databases

eggNOGiENOG4105C4S. Bacteria.
COG0481. LUCA.
HOGENOMiHOG000020624.
InParanoidiP60785.
KOiK03596.
OMAiFAGIYPV.
PhylomeDBiP60785.

Enzyme and pathway databases

BioCyciEcoCyc:EG10529-MONOMER.
ECOL316407:JW2553-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP60785.
PROiP60785.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00071. LepA. 1 hit.
InterProiIPR006297. EF-4.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEPA_ECOLI
AccessioniPrimary (citable) accession number: P60785
Secondary accession number(s): P07682, P76590, Q2MAG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 13, 2004
Last modified: September 7, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.