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Reviewed, UniProtKB/Swiss-Prot P60785 (LEPA_ECOLI)

Last modified June 16, 2009. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    GTP-binding protein lepA
Gene names
Name: lepA
Ordered Locus Names: b2569, JW2553
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length599 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Subcellular location

Cell membrane. Periplasm. HAMAP MF_00071

Miscellaneous

The overproduction of this protein is lethal to E.coli. HAMAP MF_00071

Sequence similarities

Belongs to the GTP-binding elongation factor family. LepA subfamily.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
Periplasm
   LigandGTP-binding
Nucleotide-binding
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Cellular componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionGTP binding

Inferred from electronic annotation. Source: HAMAP

GTPase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 599599GTP-binding protein lepA HAMAP MF_00071
PRO_0000176271

Regions

Nucleotide binding11 – 188GTP By similarity
Nucleotide binding77 – 815GTP By similarity
Nucleotide binding131 – 1344GTP By similarity

Experimental info

Sequence conflict156 – 18530TDAVR…PPPEG → HRRGALFSENRRWCAGRSRT SGARHSAAGS Ref.1
Sequence conflict156 – 18530TDAVR…PPPEG → HRRGALFSENRRWCAGRSRT SGARHSAAGS Ref.2
Sequence conflict2661Missing Ref.1
Sequence conflict2661Missing Ref.2

Sequences

Sequence LengthMass (Da)Tools
P60785-1 [UniParc].

Last modified April 13, 2004. Version 1.
Checksum: 91E3678557A46D49

FASTA59966,570
        10         20         30         40         50         60 
MKNIRNFSII AHIDHGKSTL SDRIIQICGG LSDREMEAQV LDSMDLERER GITIKAQSVT 

        70         80         90        100        110        120 
LDYKASDGET YQLNFIDTPG HVDFSYEVSR SLAACEGALL VVDAGQGVEA QTLANCYTAM 

       130        140        150        160        170        180 
EMDLEVVPVL NKIDLPAADP ERVAEEIEDI VGIDATDAVR CSAKTGVGVQ DVLERLVRDI 

       190        200        210        220        230        240 
PPPEGDPEGP LQALIIDSWF DNYLGVVSLI RIKNGTLRKG DKVKVMSTGQ TYNADRLGIF 

       250        260        270        280        290        300 
TPKQVDRTEL KCGEVGWLVC AIKDIHGAPV GDTLTLARNP AEKALPGFKK VKPQVYAGLF 

       310        320        330        340        350        360 
PVSSDDYEAF RDALGKLSLN DASLFYEPES SSALGFGFRC GFLGLLHMEI IQERLEREYD 

       370        380        390        400        410        420 
LDLITTAPTV VYEVETTSRE VIYVDSPSKL PAVNNIYELR EPIAECHMLL PQAYLGNVIT 

       430        440        450        460        470        480 
LCVEKRGVQT NMVYHGNQVA LTYEIPMAEV VLDFFDRLKS TSRGYASLDY NFKRFQASDM 

       490        500        510        520        530        540 
VRVDVLINGE RVDALALITH RDNSQNRGRE LVEKMKDLIP RQQFDIAIQA AIGTHIIARS 

       550        560        570        580        590 
TVKQLRKNVL AKCYGGDISR KKKLLQKQKE GKKRMKQIGN VELPQEAFLA ILHVGKDNK 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the lep operon of Escherichia coli. Identification of the promoter and the gene upstream of the signal peptidase I gene."
March P.E., Inouye M.
J. Biol. Chem. 260:7206-7213(1985) [PubMed: 2987248] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]Nashimoto H., Saito N.
Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"GTP-binding membrane protein of Escherichia coli with sequence homology to initiation factor 2 and elongation factors Tu and G."
March P.E., Inouye M.
Proc. Natl. Acad. Sci. U.S.A. 82:7500-7504(1985) [PubMed: 2999765] [Abstract]
Cited for: SIMILARITY TO GTP-BINDING PROTEINS.
[6]"Molecular cloning and sequence determination of the tuf gene coding for the elongation factor Tu of Thermus thermophilus HB8."
Kushiro A., Shimizu M., Tomita K.
Eur. J. Biochem. 170:93-98(1987) [PubMed: 2826164] [Abstract]
Cited for: SIMILARITY TO GTP-BINDING PROTEINS.
+Additional computationally mapped references.

Cross-references

Sequence databases

K00426 Genomic DNA. Translation: AAA24063.1.
D64044 Genomic DNA. Translation: BAA10916.1.
U00096 Genomic DNA. Translation: AAC75622.1.
AP009048 Genomic DNA. Translation: BAE76745.1.
PIRBVECLA. H65034.
RefSeqAP_003155.1.
NP_417064.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
3CB4X-ray2.80A/B/C/D/E/F1-599[»]
3DEGelectron microscopy-C1-545[»]
ModBaseSearch...

Genome annotation databases

GeneID947051.
GenomeReviewsGene locus JW2553 in contig AP009048_GR.
Gene locus b2569 in contig U00096_GR.
KEGGecj:JW2553.
eco:b2569.

Organism-specific databases

EchoBASEEB0524.
EcoGeneEG10529. lepA.
CMRSearch...

Phylogenomic databases

HOGENOMP60785.
OMAP60785. EVVTDFF.

Enzyme and pathway databases

BioCycEcoCyc:EG10529-MON.

Family and domain databases

HAMAPMF_00071.
[Tree]
InterProIPR006297. LepA_GTP_bd.
IPR013842. LepA_GTP_bd_C.
IPR000795. ProtSyn_GTP_bd.
IPR005225. Small_GTP_bd.
IPR000640. Transl_elong_EFG/EF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
Gene3DG3DSA:3.30.70.240. Transl_elong_EFG/EF2_C. 1 hit.
PfamPF00679. EFG_C. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
TIGRFAMsTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEPA_ECOLI
AccessionPrimary (citable) accession number: P60785
Secondary accession number(s): P07682, P76590, Q2MAG1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 13, 2004
Last modified: June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents