Reviewed, UniProtKB/Swiss-Prot P60785 (LEPA_ECOLI)

Names and origin

Protein names

Recommended name:
    GTP-binding protein lepA

Gene names

Name:	lepA
Ordered Locus Names:	b2569, JW2553

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	599 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Cell membrane. Periplasm. HAMAP MF_00071
Miscellaneous	The overproduction of this protein is lethal to E.coli. HAMAP MF_00071
Sequence similarities	Belongs to the GTP-binding elongation factor family. LepA subfamily.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Periplasm
Ligand	GTP-binding Nucleotide-binding
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 599	599	GTP-binding protein lepA HAMAP MF_00071		PRO_0000176271
Regions
	Nucleotide binding	11 – 18	8	GTP By similarity
	Nucleotide binding	77 – 81	5	GTP By similarity
	Nucleotide binding	131 – 134	4	GTP By similarity
Experimental info
	Sequence conflict	156 – 185	30	TDAVR…PPPEG → HRRGALFSENRRWCAGRSRT SGARHSAAGS Ref.1
	Sequence conflict	156 – 185	30	TDAVR…PPPEG → HRRGALFSENRRWCAGRSRT SGARHSAAGS Ref.2
	Sequence conflict	266	1	Missing Ref.1
	Sequence conflict	266	1	Missing Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P60785-1 [UniParc]. Last modified April 13, 2004. Version 1. Checksum: 91E3678557A46D49	FASTA	599	66,570
	10 20 30 40 50 60 MKNIRNFSII AHIDHGKSTL SDRIIQICGG LSDREMEAQV LDSMDLERER GITIKAQSVT 70 80 90 100 110 120 LDYKASDGET YQLNFIDTPG HVDFSYEVSR SLAACEGALL VVDAGQGVEA QTLANCYTAM 130 140 150 160 170 180 EMDLEVVPVL NKIDLPAADP ERVAEEIEDI VGIDATDAVR CSAKTGVGVQ DVLERLVRDI 190 200 210 220 230 240 PPPEGDPEGP LQALIIDSWF DNYLGVVSLI RIKNGTLRKG DKVKVMSTGQ TYNADRLGIF 250 260 270 280 290 300 TPKQVDRTEL KCGEVGWLVC AIKDIHGAPV GDTLTLARNP AEKALPGFKK VKPQVYAGLF 310 320 330 340 350 360 PVSSDDYEAF RDALGKLSLN DASLFYEPES SSALGFGFRC GFLGLLHMEI IQERLEREYD 370 380 390 400 410 420 LDLITTAPTV VYEVETTSRE VIYVDSPSKL PAVNNIYELR EPIAECHMLL PQAYLGNVIT 430 440 450 460 470 480 LCVEKRGVQT NMVYHGNQVA LTYEIPMAEV VLDFFDRLKS TSRGYASLDY NFKRFQASDM 490 500 510 520 530 540 VRVDVLINGE RVDALALITH RDNSQNRGRE LVEKMKDLIP RQQFDIAIQA AIGTHIIARS 550 560 570 580 590 TVKQLRKNVL AKCYGGDISR KKKLLQKQKE GKKRMKQIGN VELPQEAFLA ILHVGKDNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the lep operon of Escherichia coli. Identification of the promoter and the gene upstream of the signal peptidase I gene." March P.E., Inouye M. J. Biol. Chem. 260:7206-7213(1985) [PubMed: 2987248] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	Nashimoto H., Saito N. Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"GTP-binding membrane protein of Escherichia coli with sequence homology to initiation factor 2 and elongation factors Tu and G." March P.E., Inouye M. Proc. Natl. Acad. Sci. U.S.A. 82:7500-7504(1985) [PubMed: 2999765] [Abstract] Cited for: SIMILARITY TO GTP-BINDING PROTEINS.
[6]	"Molecular cloning and sequence determination of the tuf gene coding for the elongation factor Tu of Thermus thermophilus HB8." Kushiro A., Shimizu M., Tomita K. Eur. J. Biochem. 170:93-98(1987) [PubMed: 2826164] [Abstract] Cited for: SIMILARITY TO GTP-BINDING PROTEINS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

K00426 Genomic DNA. Translation: AAA24063.1.
D64044 Genomic DNA. Translation: BAA10916.1.
U00096 Genomic DNA. Translation: AAC75622.1.
AP009048 Genomic DNA. Translation: BAE76745.1.

PIR

BVECLA. H65034.

RefSeq

AP_003155.1.
NP_417064.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3CB4	X-ray	2.80	A/B/C/D/E/F	1-599	[»]
3DEG	electron microscopy	-	C	1-545	[»]

ModBase

Search...

Genome annotation databases

GeneID

947051.

GenomeReviews

Gene locus JW2553 in contig AP009048_GR.
Gene locus b2569 in contig U00096_GR.

KEGG

ecj:JW2553.
eco:b2569.

Organism-specific databases

EchoBASE

EB0524.

EcoGene

EG10529. lepA.

CMR

Search...

Phylogenomic databases

HOGENOM

P60785.

OMA

P60785. EVVTDFF.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10529-MON.

Family and domain databases

HAMAP

MF_00071.
[Tree]

InterPro

IPR006297. LepA_GTP_bd.
IPR013842. LepA_GTP_bd_C.
IPR000795. ProtSyn_GTP_bd.
IPR005225. Small_GTP_bd.
IPR000640. Transl_elong_EFG/EF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]

Gene3D

G3DSA:3.30.70.240. Transl_elong_EFG/EF2_C. 1 hit.

Pfam

PF00679. EFG_C. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]

PRINTS

PR00315. ELONGATNFCT.

TIGRFAMs

TIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.

PROSITE

PS00301. EFACTOR_GTP. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

LEPA_ECOLI

Accession

Primary (citable) accession number: P60785
Secondary accession number(s): P07682, P76590, Q2MAG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 13, 2004
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)