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Protein

Elongation factor 4

Gene

lepA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.UniRule annotation2 Publications

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 19GTPUniRule annotation6
Nucleotide bindingi131 – 134GTPUniRule annotation4

GO - Molecular functioni

  • GTPase activity Source: EcoCyc
  • GTP binding Source: UniProtKB-HAMAP
  • ribosomal large subunit binding Source: EcoCyc
  • ribosomal small subunit binding Source: EcoCyc
  • ribosome binding Source: UniProtKB-HAMAP
  • translation elongation factor activity Source: EcoCyc

GO - Biological processi

  • positive regulation of translation Source: UniProtKB-HAMAP
  • response to cold Source: EcoCyc
  • response to pH Source: EcoCyc
  • response to salt stress Source: EcoCyc
  • translational elongation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10529-MONOMER.
ECOL316407:JW2553-MONOMER.
MetaCyc:EG10529-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 4UniRule annotation (EC:3.6.5.n1UniRule annotation)
Short name:
EF-4UniRule annotation
Alternative name(s):
Ribosomal back-translocase LepAUniRule annotation
Gene namesi
Name:lepAUniRule annotation
Ordered Locus Names:b2569, JW2553
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10529. lepA.

Subcellular locationi

  • Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001762711 – 599Elongation factor 4Add BLAST599

Proteomic databases

EPDiP60785.
PaxDbiP60785.
PRIDEiP60785.

Interactioni

Protein-protein interaction databases

BioGridi4260596. 215 interactors.
DIPiDIP-29868N.
IntActiP60785. 22 interactors.
MINTiMINT-1225739.
STRINGi511145.b2569.

Structurei

Secondary structure

1599
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 10Combined sources7
Helixi20 – 27Combined sources8
Beta strandi58 – 64Combined sources7
Beta strandi70 – 77Combined sources8
Helixi82 – 84Combined sources3
Helixi85 – 94Combined sources10
Beta strandi96 – 103Combined sources8
Turni104 – 106Combined sources3
Helixi111 – 121Combined sources11
Beta strandi125 – 131Combined sources7
Helixi140 – 149Combined sources10
Beta strandi159 – 161Combined sources3
Turni163 – 165Combined sources3
Helixi169 – 179Combined sources11
Beta strandi192 – 201Combined sources10
Turni202 – 204Combined sources3
Beta strandi205 – 215Combined sources11
Beta strandi217 – 220Combined sources4
Beta strandi222 – 225Combined sources4
Turni226 – 228Combined sources3
Beta strandi231 – 233Combined sources3
Beta strandi236 – 246Combined sources11
Beta strandi248 – 250Combined sources3
Beta strandi255 – 259Combined sources5
Helixi265 – 267Combined sources3
Beta strandi273 – 279Combined sources7
Beta strandi295 – 303Combined sources9
Helixi304 – 306Combined sources3
Helixi307 – 318Combined sources12
Beta strandi325 – 331Combined sources7
Turni332 – 334Combined sources3
Beta strandi335 – 344Combined sources10
Helixi345 – 357Combined sources13
Beta strandi363 – 365Combined sources3
Beta strandi372 – 379Combined sources8
Beta strandi381 – 386Combined sources6
Helixi387 – 389Combined sources3
Helixi393 – 395Combined sources3
Beta strandi396 – 411Combined sources16
Helixi412 – 414Combined sources3
Helixi415 – 424Combined sources10
Beta strandi428 – 433Combined sources6
Beta strandi438 – 446Combined sources9
Helixi447 – 451Combined sources5
Turni452 – 454Combined sources3
Helixi455 – 461Combined sources7
Beta strandi467 – 477Combined sources11
Beta strandi480 – 487Combined sources8
Beta strandi490 – 500Combined sources11
Helixi501 – 503Combined sources3
Helixi504 – 518Combined sources15
Beta strandi526 – 532Combined sources7
Beta strandi535 – 542Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CB4X-ray2.80A/B/C/D/E/F1-599[»]
3DEGelectron microscopy-C1-545[»]
3JCDelectron microscopy3.70x1-599[»]
3JCEelectron microscopy3.20x1-599[»]
ProteinModelPortaliP60785.
SMRiP60785.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60785.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 184tr-type GAdd BLAST183

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4S. Bacteria.
COG0481. LUCA.
HOGENOMiHOG000020624.
InParanoidiP60785.
KOiK03596.
OMAiFAGIYPV.
PhylomeDBiP60785.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00071. LepA. 1 hit.
InterProiIPR006297. EF-4.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P60785-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNIRNFSII AHIDHGKSTL SDRIIQICGG LSDREMEAQV LDSMDLERER
60 70 80 90 100
GITIKAQSVT LDYKASDGET YQLNFIDTPG HVDFSYEVSR SLAACEGALL
110 120 130 140 150
VVDAGQGVEA QTLANCYTAM EMDLEVVPVL NKIDLPAADP ERVAEEIEDI
160 170 180 190 200
VGIDATDAVR CSAKTGVGVQ DVLERLVRDI PPPEGDPEGP LQALIIDSWF
210 220 230 240 250
DNYLGVVSLI RIKNGTLRKG DKVKVMSTGQ TYNADRLGIF TPKQVDRTEL
260 270 280 290 300
KCGEVGWLVC AIKDIHGAPV GDTLTLARNP AEKALPGFKK VKPQVYAGLF
310 320 330 340 350
PVSSDDYEAF RDALGKLSLN DASLFYEPES SSALGFGFRC GFLGLLHMEI
360 370 380 390 400
IQERLEREYD LDLITTAPTV VYEVETTSRE VIYVDSPSKL PAVNNIYELR
410 420 430 440 450
EPIAECHMLL PQAYLGNVIT LCVEKRGVQT NMVYHGNQVA LTYEIPMAEV
460 470 480 490 500
VLDFFDRLKS TSRGYASLDY NFKRFQASDM VRVDVLINGE RVDALALITH
510 520 530 540 550
RDNSQNRGRE LVEKMKDLIP RQQFDIAIQA AIGTHIIARS TVKQLRKNVL
560 570 580 590
AKCYGGDISR KKKLLQKQKE GKKRMKQIGN VELPQEAFLA ILHVGKDNK
Length:599
Mass (Da):66,570
Last modified:April 13, 2004 - v1
Checksum:i91E3678557A46D49
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti156 – 185TDAVR…PPPEG → HRRGALFSENRRWCAGRSRT SGARHSAAGS (PubMed:2987248).CuratedAdd BLAST30
Sequence conflicti156 – 185TDAVR…PPPEG → HRRGALFSENRRWCAGRSRT SGARHSAAGS (Ref. 2) CuratedAdd BLAST30
Sequence conflicti266Missing (PubMed:2987248).Curated1
Sequence conflicti266Missing (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00426 Genomic DNA. Translation: AAA24063.1.
D64044 Genomic DNA. Translation: BAA10916.1.
U00096 Genomic DNA. Translation: AAC75622.1.
AP009048 Genomic DNA. Translation: BAE76745.1.
PIRiH65034. BVECLA.
RefSeqiNP_417064.1. NC_000913.3.
WP_000790168.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75622; AAC75622; b2569.
BAE76745; BAE76745; BAE76745.
GeneIDi947051.
KEGGiecj:JW2553.
eco:b2569.
PATRICi32120537. VBIEscCol129921_2671.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00426 Genomic DNA. Translation: AAA24063.1.
D64044 Genomic DNA. Translation: BAA10916.1.
U00096 Genomic DNA. Translation: AAC75622.1.
AP009048 Genomic DNA. Translation: BAE76745.1.
PIRiH65034. BVECLA.
RefSeqiNP_417064.1. NC_000913.3.
WP_000790168.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CB4X-ray2.80A/B/C/D/E/F1-599[»]
3DEGelectron microscopy-C1-545[»]
3JCDelectron microscopy3.70x1-599[»]
3JCEelectron microscopy3.20x1-599[»]
ProteinModelPortaliP60785.
SMRiP60785.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260596. 215 interactors.
DIPiDIP-29868N.
IntActiP60785. 22 interactors.
MINTiMINT-1225739.
STRINGi511145.b2569.

Proteomic databases

EPDiP60785.
PaxDbiP60785.
PRIDEiP60785.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75622; AAC75622; b2569.
BAE76745; BAE76745; BAE76745.
GeneIDi947051.
KEGGiecj:JW2553.
eco:b2569.
PATRICi32120537. VBIEscCol129921_2671.

Organism-specific databases

EchoBASEiEB0524.
EcoGeneiEG10529. lepA.

Phylogenomic databases

eggNOGiENOG4105C4S. Bacteria.
COG0481. LUCA.
HOGENOMiHOG000020624.
InParanoidiP60785.
KOiK03596.
OMAiFAGIYPV.
PhylomeDBiP60785.

Enzyme and pathway databases

BioCyciEcoCyc:EG10529-MONOMER.
ECOL316407:JW2553-MONOMER.
MetaCyc:EG10529-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP60785.
PROiP60785.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00071. LepA. 1 hit.
InterProiIPR006297. EF-4.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEPA_ECOLI
AccessioniPrimary (citable) accession number: P60785
Secondary accession number(s): P07682, P76590, Q2MAG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 13, 2004
Last modified: November 2, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.