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Protein

Probable glycerol-3-phosphate acyltransferase

Gene

plsY

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of an acyl group from acyl-ACP to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme can also utilize acyl-CoA as fatty acyl donor, but not acyl-PO4 (Probable).1 Publication

Catalytic activityi

Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.1 Publication
Acyl-[acyl-carrier-protein] + sn-glycerol 3-phosphate = [acyl-carrier-protein] + 1-acyl-sn-glycerol 3-phosphate.1 Publication

Pathwayi: phospholipid metabolism

This protein is involved in the pathway phospholipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway phospholipid metabolism and in Lipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciEcoCyc:EG11674-MONOMER.
ECOL316407:JW3031-MONOMER.
UniPathwayiUPA00085.

Protein family/group databases

TCDBi9.B.31.1.1. the yqih (yqih) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glycerol-3-phosphate acyltransferase
Alternative name(s):
G3P acyltransferase (EC:2.3.1.15, EC:2.3.1.n5)
Short name:
GPAT
Lysophosphatidic acid synthase
Short name:
LPA synthase
Gene namesi
Name:plsY
Synonyms:ygiH
Ordered Locus Names:b3059, JW3031
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11674. plsY.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 33PeriplasmicSequence analysis
Transmembranei4 – 2421HelicalSequence analysisAdd
BLAST
Topological domaini25 – 5228CytoplasmicSequence analysisAdd
BLAST
Transmembranei53 – 7321HelicalSequence analysisAdd
BLAST
Topological domaini74 – 807PeriplasmicSequence analysis
Transmembranei81 – 10121HelicalSequence analysisAdd
BLAST
Topological domaini102 – 11110CytoplasmicSequence analysis
Transmembranei112 – 13221HelicalSequence analysisAdd
BLAST
Topological domaini133 – 1375PeriplasmicSequence analysis
Transmembranei138 – 15821HelicalSequence analysisAdd
BLAST
Topological domaini159 – 20547CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 205205Probable glycerol-3-phosphate acyltransferasePRO_0000188368Add
BLAST

Proteomic databases

PaxDbiP60782.

Interactioni

Subunit structurei

Probably interacts with PlsX.By similarity

Protein-protein interaction databases

BioGridi4259257. 259 interactions.
IntActiP60782. 1 interaction.
STRINGi511145.b3059.

Structurei

3D structure databases

ProteinModelPortaliP60782.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PlsY family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105K7Z. Bacteria.
COG0344. LUCA.
HOGENOMiHOG000283806.
InParanoidiP60782.
KOiK08591.
OMAiHIWPVWL.
OrthoDBiEOG6M6JSX.
PhylomeDBiP60782.

Family and domain databases

HAMAPiMF_01043. PlsY.
InterProiIPR003811. G3P_acylTferase_PlsY.
[Graphical view]
PANTHERiPTHR30309:SF0. PTHR30309:SF0. 1 hit.
PfamiPF02660. G3P_acyltransf. 1 hit.
[Graphical view]
SMARTiSM01207. G3P_acyltransf. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00023. TIGR00023. 1 hit.

Sequencei

Sequence statusi: Complete.

P60782-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAIAPGMIL IAYLCGSISS AILVCRLCGL PDPRTSGSGN PGATNVLRIG
60 70 80 90 100
GKGAAVAVLI FDVLKGMLPV WGAYELGVSP FWLGLIAIAA CLGHIWPVFF
110 120 130 140 150
GFKGGKGVAT AFGAIAPIGW DLTGVMAGTW LLTVLLSGYS SLGAIVSALI
160 170 180 190 200
APFYVWWFKP QFTFPVSMLS CLILLRHHDN IQRLWRRQET KIWTKFKRKR

EKDPE
Length:205
Mass (Da):22,193
Last modified:April 13, 2004 - v1
Checksum:i13066C8FBA2543E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28379 Genomic DNA. Translation: AAA89139.1.
U00096 Genomic DNA. Translation: AAC76095.1.
AP009048 Genomic DNA. Translation: BAE77110.1.
L12966 Genomic DNA. No translation available.
PIRiA65094.
RefSeqiNP_417531.1. NC_000913.3.
WP_001272796.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76095; AAC76095; b3059.
BAE77110; BAE77110; BAE77110.
GeneIDi947561.
KEGGiecj:JW3031.
eco:b3059.
PATRICi32121530. VBIEscCol129921_3152.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28379 Genomic DNA. Translation: AAA89139.1.
U00096 Genomic DNA. Translation: AAC76095.1.
AP009048 Genomic DNA. Translation: BAE77110.1.
L12966 Genomic DNA. No translation available.
PIRiA65094.
RefSeqiNP_417531.1. NC_000913.3.
WP_001272796.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP60782.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259257. 259 interactions.
IntActiP60782. 1 interaction.
STRINGi511145.b3059.

Protein family/group databases

TCDBi9.B.31.1.1. the yqih (yqih) family.

Proteomic databases

PaxDbiP60782.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76095; AAC76095; b3059.
BAE77110; BAE77110; BAE77110.
GeneIDi947561.
KEGGiecj:JW3031.
eco:b3059.
PATRICi32121530. VBIEscCol129921_3152.

Organism-specific databases

EchoBASEiEB1625.
EcoGeneiEG11674. plsY.

Phylogenomic databases

eggNOGiENOG4105K7Z. Bacteria.
COG0344. LUCA.
HOGENOMiHOG000283806.
InParanoidiP60782.
KOiK08591.
OMAiHIWPVWL.
OrthoDBiEOG6M6JSX.
PhylomeDBiP60782.

Enzyme and pathway databases

UniPathwayiUPA00085.
BioCyciEcoCyc:EG11674-MONOMER.
ECOL316407:JW3031-MONOMER.

Miscellaneous databases

PROiP60782.

Family and domain databases

HAMAPiMF_01043. PlsY.
InterProiIPR003811. G3P_acylTferase_PlsY.
[Graphical view]
PANTHERiPTHR30309:SF0. PTHR30309:SF0. 1 hit.
PfamiPF02660. G3P_acyltransf. 1 hit.
[Graphical view]
SMARTiSM01207. G3P_acyltransf. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00023. TIGR00023. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Amplification of the bacA gene confers bacitracin resistance to Escherichia coli."
    Cain B.D., Norton P.J., Eubanks W., Nick H.S., Allen C.M.
    J. Bacteriol. 175:3784-3789(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-204.
    Strain: K12 / ATCC 35607 / JM83.
  4. "Intrinsic and extrinsic approaches for detecting genes in a bacterial genome."
    Borodovsky M., Rudd K.E., Koonin E.V.
    Nucleic Acids Res. 22:4756-4767(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  6. "Acyl-phosphates initiate membrane phospholipid synthesis in Gram-positive pathogens."
    Lu Y.-J., Zhang Y.-M., Grimes K.D., Qi J., Lee R.E., Rock C.O.
    Mol. Cell 23:765-772(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, FATTY ACYL DONOR SPECIFICITY.
  7. "Involvement of the YneS/YgiH and PlsX proteins in phospholipid biosynthesis in both Bacillus subtilis and Escherichia coli."
    Yoshimura M., Oshima T., Ogasawara N.
    BMC Microbiol. 7:69-69(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN PHOSPHOLIPID BIOSYNTHESIS.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiPLSY_ECOLI
AccessioniPrimary (citable) accession number: P60782
Secondary accession number(s): P31056, Q2M9E6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: May 11, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.