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Protein

Ribonuclease P protein component 3

Gene

rnp3

Organism
Methanococcus maripaludis (strain S2 / LL)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.UniRule annotation1 Publication

Catalytic activityi

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.UniRule annotation

Kineticsi

kcat 10 min(-1) in absence of L7Ae, 63 min(-1) in presence of L7Ae. Kinetic parameters determined at 37 degrees Celsius.

  1. KM=2.6 µM for pre-tRNA-Tyr in the absence of L7Ae1 Publication
  2. KM=0.044 µM for pre-tRNA-Tyr in the presence of L7Ae1 Publication

Temperature dependencei

Optimum temperature is 36-38 degrees Celsius in the absence of L7Ae, 48-50 degrees Celsius in presence of L7Ae.1 Publication

GO - Molecular functioni

  1. ribonuclease P activity Source: UniProtKB

GO - Biological processi

  1. RNA phosphodiester bond hydrolysis Source: GOC
  2. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
  3. tRNA 5'-leader removal Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciMMAR267377:GJ77-457-MONOMER.
BRENDAi3.1.26.5. 3262.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease P protein component 3UniRule annotation (EC:3.1.26.5UniRule annotation)
Short name:
RNase P component 3UniRule annotation
Alternative name(s):
Rpp30UniRule annotation
Gene namesi
Name:rnp3UniRule annotation
Ordered Locus Names:MMP0430
OrganismiMethanococcus maripaludis (strain S2 / LL)
Taxonomic identifieri267377 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
ProteomesiUP000000590 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. ribonuclease P complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 232232Ribonuclease P protein component 3PRO_0000140042Add
BLAST

Interactioni

Subunit structurei

Consists of a catalytic RNA component and at least 5 protein subunits.1 Publication

Protein-protein interaction databases

DIPiDIP-59366N.
STRINGi267377.MMP0430.

Structurei

3D structure databases

ProteinModelPortaliP60781.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic/archaeal RNase P protein component 3 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1603.
HOGENOMiHOG000296348.
KOiK03539.
OMAiVAIELNF.

Family and domain databases

HAMAPiMF_00756. RNase_P_3.
InterProiIPR016195. Pol/histidinol_Pase-like.
IPR023539. RNase_P_comp-3_arc.
IPR002738. RNase_P_p30.
[Graphical view]
PfamiPF01876. RNase_P_p30. 1 hit.
[Graphical view]
SUPFAMiSSF89550. SSF89550. 1 hit.

Sequencei

Sequence statusi: Complete.

P60781-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEGIFDINH VFDEEGIKTL KRFGWDGSVA VQNHNEYSEE KINTAVEYGE
60 70 80 90 100
NCEFKVYSGV KISTKNQNEM EKAVKKYRNK VDILLVEGGD VKINRRVLEM
110 120 130 140 150
NDVDILSTPE LNRMDNGLDH ILARLGSTNR VAVELNFGNL LKSKNYDRSK
160 170 180 190 200
ILWAFQRNLK LSKKYDTPVV ISSGANDIYG IKAPGDLRGF LNTVADPLYS
210 220 230
KKIIETTSKI IDYRLYLKNK NVLMPGLEIV EE
Length:232
Mass (Da):26,452
Last modified:April 12, 2004 - v1
Checksum:i1BE3FFFB9096C7EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950229 Genomic DNA. Translation: CAF29986.1.
RefSeqiNP_987550.1. NC_005791.1.
WP_011170374.1. NC_005791.1.

Genome annotation databases

EnsemblBacteriaiCAF29986; CAF29986; MMP0430.
GeneIDi2762077.
KEGGimmp:MMP0430.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950229 Genomic DNA. Translation: CAF29986.1.
RefSeqiNP_987550.1. NC_005791.1.
WP_011170374.1. NC_005791.1.

3D structure databases

ProteinModelPortaliP60781.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59366N.
STRINGi267377.MMP0430.

Protocols and materials databases

DNASUi2762077.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAF29986; CAF29986; MMP0430.
GeneIDi2762077.
KEGGimmp:MMP0430.

Phylogenomic databases

eggNOGiCOG1603.
HOGENOMiHOG000296348.
KOiK03539.
OMAiVAIELNF.

Enzyme and pathway databases

BioCyciMMAR267377:GJ77-457-MONOMER.
BRENDAi3.1.26.5. 3262.

Family and domain databases

HAMAPiMF_00756. RNase_P_3.
InterProiIPR016195. Pol/histidinol_Pase-like.
IPR023539. RNase_P_comp-3_arc.
IPR002738. RNase_P_p30.
[Graphical view]
PfamiPF01876. RNase_P_p30. 1 hit.
[Graphical view]
SUPFAMiSSF89550. SSF89550. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: S2 / LL.
  2. Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION.
    Strain: S2 / LL.

Entry informationi

Entry nameiRNP3_METMP
AccessioniPrimary (citable) accession number: P60781
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2004
Last sequence update: April 12, 2004
Last modified: March 31, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.