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Protein

Ribonuclease P protein component 3

Gene

rnp3

Organism
Methanococcus maripaludis (strain S2 / LL)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.UniRule annotation1 Publication

Catalytic activityi

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.UniRule annotation

Kineticsi

kcat 10 min(-1) in absence of L7Ae, 63 min(-1) in presence of L7Ae. Kinetic parameters determined at 37 degrees Celsius.

  1. KM=2.6 µM for pre-tRNA-Tyr in the absence of L7Ae1 Publication
  2. KM=0.044 µM for pre-tRNA-Tyr in the presence of L7Ae1 Publication

    Temperature dependencei

    Optimum temperature is 36-38 degrees Celsius in the absence of L7Ae, 48-50 degrees Celsius in presence of L7Ae.1 Publication

    GO - Molecular functioni

    • ribonuclease P activity Source: UniProtKB

    GO - Biological processi

    • RNA phosphodiester bond hydrolysis Source: GOC
    • RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
    • tRNA 5'-leader removal Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    tRNA processing

    Enzyme and pathway databases

    BioCyciMMAR267377:GJ77-457-MONOMER.
    BRENDAi3.1.26.5. 3262.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease P protein component 3UniRule annotation (EC:3.1.26.5UniRule annotation)
    Short name:
    RNase P component 3UniRule annotation
    Alternative name(s):
    Rpp30UniRule annotation
    Gene namesi
    Name:rnp3UniRule annotation
    Ordered Locus Names:MMP0430
    OrganismiMethanococcus maripaludis (strain S2 / LL)
    Taxonomic identifieri267377 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
    ProteomesiUP000000590 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: UniProtKB
    • ribonuclease P complex Source: UniProtKB
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 232232Ribonuclease P protein component 3PRO_0000140042Add
    BLAST

    Interactioni

    Subunit structurei

    Consists of a catalytic RNA component and at least 5 protein subunits.1 Publication

    Protein-protein interaction databases

    DIPiDIP-59366N.
    STRINGi267377.MMP0430.

    Structurei

    3D structure databases

    ProteinModelPortaliP60781.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the eukaryotic/archaeal RNase P protein component 3 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1603.
    HOGENOMiHOG000296348.
    KOiK03539.
    OMAiVAIELNF.

    Family and domain databases

    HAMAPiMF_00756. RNase_P_3.
    InterProiIPR016195. Pol/histidinol_Pase-like.
    IPR023539. RNase_P_comp-3_arc.
    IPR002738. RNase_P_p30.
    [Graphical view]
    PfamiPF01876. RNase_P_p30. 1 hit.
    [Graphical view]
    SUPFAMiSSF89550. SSF89550. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P60781-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MLEGIFDINH VFDEEGIKTL KRFGWDGSVA VQNHNEYSEE KINTAVEYGE
    60 70 80 90 100
    NCEFKVYSGV KISTKNQNEM EKAVKKYRNK VDILLVEGGD VKINRRVLEM
    110 120 130 140 150
    NDVDILSTPE LNRMDNGLDH ILARLGSTNR VAVELNFGNL LKSKNYDRSK
    160 170 180 190 200
    ILWAFQRNLK LSKKYDTPVV ISSGANDIYG IKAPGDLRGF LNTVADPLYS
    210 220 230
    KKIIETTSKI IDYRLYLKNK NVLMPGLEIV EE
    Length:232
    Mass (Da):26,452
    Last modified:April 13, 2004 - v1
    Checksum:i1BE3FFFB9096C7EA
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BX950229 Genomic DNA. Translation: CAF29986.1.
    RefSeqiNP_987550.1. NC_005791.1.
    WP_011170374.1. NC_005791.1.

    Genome annotation databases

    EnsemblBacteriaiCAF29986; CAF29986; MMP0430.
    GeneIDi2762077.
    KEGGimmp:MMP0430.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BX950229 Genomic DNA. Translation: CAF29986.1.
    RefSeqiNP_987550.1. NC_005791.1.
    WP_011170374.1. NC_005791.1.

    3D structure databases

    ProteinModelPortaliP60781.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    DIPiDIP-59366N.
    STRINGi267377.MMP0430.

    Protocols and materials databases

    DNASUi2762077.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAF29986; CAF29986; MMP0430.
    GeneIDi2762077.
    KEGGimmp:MMP0430.

    Phylogenomic databases

    eggNOGiCOG1603.
    HOGENOMiHOG000296348.
    KOiK03539.
    OMAiVAIELNF.

    Enzyme and pathway databases

    BioCyciMMAR267377:GJ77-457-MONOMER.
    BRENDAi3.1.26.5. 3262.

    Family and domain databases

    HAMAPiMF_00756. RNase_P_3.
    InterProiIPR016195. Pol/histidinol_Pase-like.
    IPR023539. RNase_P_comp-3_arc.
    IPR002738. RNase_P_p30.
    [Graphical view]
    PfamiPF01876. RNase_P_p30. 1 hit.
    [Graphical view]
    SUPFAMiSSF89550. SSF89550. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: S2 / LL.
    2. Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION.
      Strain: S2 / LL.

    Entry informationi

    Entry nameiRNP3_METMP
    AccessioniPrimary (citable) accession number: P60781
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: April 13, 2004
    Last modified: May 27, 2015
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.