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Protein

Ribonuclease P protein component 2

Gene

rnp2

Organism
Methanococcus maripaludis (strain S2 / LL)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.UniRule annotation1 Publication

Catalytic activityi

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.UniRule annotation

Kineticsi

kcat 10 min(-1) in absence of L7Ae, 63 min(-1) in presence of L7Ae. Kinetic parameters determined at 37 degrees Celsius.
  1. KM=2.6 µM for pre-tRNA-Tyr in the absence of L7Ae1 Publication
  2. KM=0.044 µM for pre-tRNA-Tyr in the presence of L7Ae1 Publication

    Temperature dependencei

    Optimum temperature is 36-38 degrees Celsius in the absence of L7Ae, 48-50 degrees Celsius in presence of L7Ae.1 Publication

    GO - Molecular functioni

    • ribonuclease P activity Source: UniProtKB

    GO - Biological processi

    • tRNA 5'-leader removal Source: UniProtKB

    Keywordsi

    Molecular functionEndonuclease, Hydrolase, Nuclease
    Biological processtRNA processing

    Enzyme and pathway databases

    BioCyciMMAR267377:G1G3S-916-MONOMER
    BRENDAi3.1.26.5 3262

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease P protein component 2UniRule annotation (EC:3.1.26.5UniRule annotation)
    Short name:
    RNase P component 2UniRule annotation
    Alternative name(s):
    Pop5UniRule annotation
    Gene namesi
    Name:rnp2UniRule annotation
    Ordered Locus Names:MMP0878
    OrganismiMethanococcus maripaludis (strain S2 / LL)
    Taxonomic identifieri267377 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
    Proteomesi
    • UP000000590 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: UniProtKB
    • ribonuclease P complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001400231 – 130Ribonuclease P protein component 2Add BLAST130

    Proteomic databases

    PRIDEiP60780

    Interactioni

    Subunit structurei

    Consists of a catalytic RNA component and at least 5 protein subunits.1 Publication

    Protein-protein interaction databases

    DIPiDIP-59368N
    IntActiP60780, 1 interactor
    STRINGi267377.MMP0878

    Structurei

    3D structure databases

    ProteinModelPortaliP60780
    SMRiP60780
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the eukaryotic/archaeal RNase P protein component 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiarCOG01365 Archaea
    COG1369 LUCA
    HOGENOMiHOG000286243
    KOiK03537
    OMAiNPWLIDY
    OrthoDBiPOG093Z0EPO

    Family and domain databases

    Gene3Di3.30.70.3250, 1 hit
    HAMAPiMF_00755 RNase_P_2, 1 hit
    InterProiView protein in InterPro
    IPR002759 RNase_P/MRP_subunit
    IPR016434 RNase_P_comp-2
    IPR038085 Rnp2-like_sf
    PfamiView protein in Pfam
    PF01900 RNase_P_Rpp14, 1 hit
    PIRSFiPIRSF004952 RNase_P_2, 1 hit
    SUPFAMiSSF160350 SSF160350, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P60780-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MLKTLPPTLR EKKRYVALEI IYEMELSQKD VISVVRNALL NYSGVLGCSR
    60 70 80 90 100
    TNPWLIDYGH PYGILRISRE EVDTLRSSLS LMGEHKKKPI NIRIIGISNS
    110 120 130
    VKHIREKFLH VPHEPYYKVI QKLKRKGPKK
    Length:130
    Mass (Da):15,149
    Last modified:April 13, 2004 - v1
    Checksum:iF886926B406FCA75
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BX950229 Genomic DNA Translation: CAF30434.1
    RefSeqiWP_011170822.1, NC_005791.1

    Genome annotation databases

    EnsemblBacteriaiCAF30434; CAF30434; MMP0878
    GeneIDi2762695
    KEGGimmp:MMP0878
    PATRICifig|267377.15.peg.904

    Similar proteinsi

    Entry informationi

    Entry nameiRNP2_METMP
    AccessioniPrimary (citable) accession number: P60780
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: April 13, 2004
    Last modified: May 23, 2018
    This is version 68 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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