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Protein

Ribonuclease P protein component 2

Gene

rnp2

Organism
Methanococcus maripaludis (strain S2 / LL)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.UniRule annotation1 Publication

Catalytic activityi

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.UniRule annotation

Kineticsi

kcat 10 min(-1) in absence of L7Ae, 63 min(-1) in presence of L7Ae. Kinetic parameters determined at 37 degrees Celsius.

  1. KM=2.6 µM for pre-tRNA-Tyr in the absence of L7Ae1 Publication
  2. KM=0.044 µM for pre-tRNA-Tyr in the presence of L7Ae1 Publication

Temperature dependencei

Optimum temperature is 36-38 degrees Celsius in the absence of L7Ae, 48-50 degrees Celsius in presence of L7Ae.1 Publication

GO - Molecular functioni

  1. ribonuclease P activity Source: UniProtKB

GO - Biological processi

  1. RNA phosphodiester bond hydrolysis Source: GOC
  2. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
  3. tRNA 5'-leader removal Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciMMAR267377:GJ77-908-MONOMER.
BRENDAi3.1.26.5. 3262.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease P protein component 2UniRule annotation (EC:3.1.26.5UniRule annotation)
Short name:
RNase P component 2UniRule annotation
Alternative name(s):
Pop5UniRule annotation
Gene namesi
Name:rnp2UniRule annotation
Ordered Locus Names:MMP0878
OrganismiMethanococcus maripaludis (strain S2 / LL)
Taxonomic identifieri267377 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
ProteomesiUP000000590 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. ribonuclease P complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 130130Ribonuclease P protein component 2PRO_0000140023Add
BLAST

Interactioni

Subunit structurei

Consists of a catalytic RNA component and at least 5 protein subunits.1 Publication

Protein-protein interaction databases

DIPiDIP-59368N.
STRINGi267377.MMP0878.

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic/archaeal RNase P protein component 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1369.
HOGENOMiHOG000286243.
KOiK03537.
OMAiNPWLIDY.

Family and domain databases

HAMAPiMF_00755. RNase_P_2.
InterProiIPR002759. RNase_P/MRP_subunit.
IPR016434. RNase_P_comp-2.
[Graphical view]
PfamiPF01900. RNase_P_Rpp14. 1 hit.
[Graphical view]
PIRSFiPIRSF004952. RNase_P_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P60780-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKTLPPTLR EKKRYVALEI IYEMELSQKD VISVVRNALL NYSGVLGCSR
60 70 80 90 100
TNPWLIDYGH PYGILRISRE EVDTLRSSLS LMGEHKKKPI NIRIIGISNS
110 120 130
VKHIREKFLH VPHEPYYKVI QKLKRKGPKK
Length:130
Mass (Da):15,149
Last modified:April 13, 2004 - v1
Checksum:iF886926B406FCA75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950229 Genomic DNA. Translation: CAF30434.1.
RefSeqiNP_987998.1. NC_005791.1.
WP_011170822.1. NC_005791.1.

Genome annotation databases

EnsemblBacteriaiCAF30434; CAF30434; MMP0878.
GeneIDi2762695.
KEGGimmp:MMP0878.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950229 Genomic DNA. Translation: CAF30434.1.
RefSeqiNP_987998.1. NC_005791.1.
WP_011170822.1. NC_005791.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59368N.
STRINGi267377.MMP0878.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAF30434; CAF30434; MMP0878.
GeneIDi2762695.
KEGGimmp:MMP0878.

Phylogenomic databases

eggNOGiCOG1369.
HOGENOMiHOG000286243.
KOiK03537.
OMAiNPWLIDY.

Enzyme and pathway databases

BioCyciMMAR267377:GJ77-908-MONOMER.
BRENDAi3.1.26.5. 3262.

Family and domain databases

HAMAPiMF_00755. RNase_P_2.
InterProiIPR002759. RNase_P/MRP_subunit.
IPR016434. RNase_P_comp-2.
[Graphical view]
PfamiPF01900. RNase_P_Rpp14. 1 hit.
[Graphical view]
PIRSFiPIRSF004952. RNase_P_2. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: S2 / LL.
  2. Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION.
    Strain: S2 / LL.

Entry informationi

Entry nameiRNP2_METMP
AccessioniPrimary (citable) accession number: P60780
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: April 1, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.