P60773 (NXS1_NAJPH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Short neurotoxin 1 Short name=NTX I
Organism	Naja philippinensis (Philippine cobra)
Taxonomic identifier	8659 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Elapidae › Elapinae › Naja

Protein attributes

Sequence length	61 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site. Binds to the muscular nicotinic acetylcholine receptor.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Toxic dose	LD50 is 0.05 mg/kg by intravenous injection.
Sequence similarities	Belongs to the snake three-finger toxin family. Type I alpha-neurotoxin subfamily.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Acetylcholine receptor inhibiting toxin Neurotoxin Postsynaptic neurotoxin Toxin
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	modification of morphology or physiology of other organism Inferred from electronic annotation. Source: InterPro
Cellular_component	other organism postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	acetylcholine receptor inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 61	61	Short neurotoxin 1		PRO_0000093609
Amino acid modifications
	Disulfide bond	3 ↔ 23		By similarity
	Disulfide bond	17 ↔ 40		By similarity
	Disulfide bond	42 ↔ 53		By similarity
	Disulfide bond	54 ↔ 59		By similarity
Natural variations
	Natural variant	55	1	R → T in minor component.

Sequences

	Sequence		Length	Mass (Da)
	P60773 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 74A0EA75BE568961	FASTA	61	6,873
	10 20 30 40 50 60 LECHNQQSSQ APTTKTCSGE TNCYKKWWSD HRGTIIERGC GCPKVKPGVK LNCCRTDRCN N

References

[1]	"The major lethal neurotoxin of the venom of Naja naja philippinensis. Purification, physical and chemical properties, partial amino acid sequence." Hauert J., Maire M., Sussmann A., Bargetzi J.-P. Int. J. Pept. Protein Res. 6:201-222(1974) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE (MAJOR COMPONENT). Tissue: Venom.
[2]	Hauert J. Thesis (1977), University of Geneva, Switzerland Cited for: PROTEIN SEQUENCE (MINOR COMPONENT). Tissue: Venom.

Cross-references

Sequence databases
PIR	N1NJ1P. A91768. N1NJ2P. B94448.
3D structure databases
ProteinModelPortal	P60773.
SMR	P60773. Positions 1-61.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG006553.
Family and domain databases
InterPro	IPR003571. Snake_toxin. IPR018354. Snake_toxin_BS. [Graphical view]
Pfam	PF00087. Toxin_1. 1 hit. [Graphical view]
PROSITE	PS00272. SNAKE_TOXIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NXS1_NAJPH

Accession

Primary (citable) accession number: P60773
Secondary accession number(s): P01428

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Annotation program

Animal Toxin Annotation Program