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Protein

Cobrotoxin

Gene
N/A
Organism
Naja atra (Chinese cobra)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to muscle nicotinic acetylcholine receptor (nAChR) and inhibit acetylcholine from binding to the receptor, thereby impairing neuromuscular transmission. Has a higher toxicity than cobrotoxin-b.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei54May be critical for toxicity1
Sitei57May be critical for toxicity1

GO - Biological processi

Keywordsi

Molecular functionAcetylcholine receptor inhibiting toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Cobrotoxin
Short name:
CBT
Alternative name(s):
Atratoxin
Short neurotoxin 1
OrganismiNaja atra (Chinese cobra)
Taxonomic identifieri8656 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeNaja

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 0.09 mg/kg by subcutaneous injection.

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi59E → Q: Decrease in the rate of isomerization reaction of disulfide bonds. 1 Publication1
Mutagenesisi68K → E: Decrease in the rate of isomerization reaction of disulfide bonds. 1 Publication1
Mutagenesisi68K → Q: Decrease in the rate of isomerization reaction of disulfide bonds. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 214 PublicationsAdd BLAST21
ChainiPRO_000003545222 – 83CobrotoxinAdd BLAST62

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi24 ↔ 45
Disulfide bondi38 ↔ 62
Disulfide bondi64 ↔ 75
Disulfide bondi76 ↔ 81

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP60770.

Expressioni

Tissue specificityi

Expressed by the venom gland.Curated

Structurei

Secondary structure

183
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi23 – 25Combined sources3
Beta strandi35 – 37Combined sources3
Beta strandi45 – 52Combined sources8
Beta strandi55 – 63Combined sources9
Beta strandi72 – 78Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1COENMR-A22-83[»]
1V6PX-ray0.87A/B22-83[»]
ProteinModelPortaliP60770.
SMRiP60770.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60770.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG006553.

Family and domain databases

CDDicd00206. snake_toxin. 1 hit.
InterProiView protein in InterPro
IPR003571. Snake_3FTx.
IPR018354. Snake_toxin_con_site.
IPR035076. Toxin/TOLIP.
PfamiView protein in Pfam
PF00087. Toxin_TOLIP. 1 hit.
PROSITEiView protein in PROSITE
PS00272. SNAKE_TOXIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60770-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTLLLTLLV VTIVCLDLGY TLECHNQQSS QTPTTTGCSG GETNCYKKRW
60 70 80
RDHRGYRTER GCGCPSVKNG IEINCCTTDR CNN
Length:83
Mass (Da):9,262
Last modified:April 13, 2004 - v1
Checksum:i4DD6077C92717052
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2K → E in AAG43384 (Ref. 4) Curated1
Sequence conflicti52D → Y in CAB43097 (PubMed:10512733).Curated1
Sequence conflicti66S → I in AAG43384 (Ref. 4) Curated1
Sequence conflicti73I → S in AAG43384 (Ref. 4) Curated1
Sequence conflicti79 – 80DR → G in CAB43097 (PubMed:10512733).Curated2

Mass spectrometryi

Molecular mass is 6952 Da from positions 22 - 83. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58519 mRNA. Translation: AAB03221.1.
U58520 mRNA. Translation: AAB03222.1.
U58521 mRNA. Translation: AAB03223.1.
U77490 mRNA. Translation: AAB36930.1.
U77491 mRNA. Translation: AAB36931.1.
U77492 mRNA. Translation: AAB36932.1.
U42582 mRNA. Translation: AAB01538.1.
Y12492 Genomic DNA. Translation: CAA73097.2.
AF161797 Genomic DNA. Translation: AAG43384.1.
AJ239050 mRNA. Translation: CAB43097.1.
AY471578 mRNA. Translation: AAR33035.1.
PIRiJC5769. N1NJ1F.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry namei3S1CB_NAJAT
AccessioniPrimary (citable) accession number: P60770
Secondary accession number(s): O13079
, P01430, Q675L7, Q9DE57, Q9W6X0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 13, 2004
Last modified: July 5, 2017
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families