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Protein

Cobrotoxin

Gene
N/A
Organism
Naja atra (Chinese cobra)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to muscle nicotinic acetylcholine receptor (nAChR) and inhibit acetylcholine from binding to the receptor, thereby impairing neuromuscular transmission. Has a higher toxicity than cobrotoxin-b.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei54 – 541May be critical for toxicity
Sitei57 – 571May be critical for toxicity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acetylcholine receptor inhibiting toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Cobrotoxin
Short name:
CBT
Alternative name(s):
Atratoxin
Short neurotoxin 1
OrganismiNaja atra (Chinese cobra)
Taxonomic identifieri8656 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeNaja

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 0.09 mg/kg by subcutaneous injection.

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi59 – 591E → Q: Decrease in the rate of isomerization reaction of disulfide bonds. 1 Publication
Mutagenesisi68 – 681K → E: Decrease in the rate of isomerization reaction of disulfide bonds. 1 Publication
Mutagenesisi68 – 681K → Q: Decrease in the rate of isomerization reaction of disulfide bonds. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21214 PublicationsAdd
BLAST
Chaini22 – 8362CobrotoxinPRO_0000035452Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi24 ↔ 45
Disulfide bondi38 ↔ 62
Disulfide bondi64 ↔ 75
Disulfide bondi76 ↔ 81

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.Curated

Structurei

Secondary structure

1
83
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi23 – 253Combined sources
Beta strandi35 – 373Combined sources
Beta strandi45 – 528Combined sources
Beta strandi55 – 639Combined sources
Beta strandi72 – 787Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1COENMR-A22-83[»]
1V6PX-ray0.87A/B22-83[»]
ProteinModelPortaliP60770.
SMRiP60770. Positions 22-83.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60770.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG006553.

Family and domain databases

CDDicd00206. snake_toxin. 1 hit.
InterProiIPR003571. Snake_3FTx.
IPR018354. Snake_toxin_con_site.
[Graphical view]
PROSITEiPS00272. SNAKE_TOXIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60770-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTLLLTLLV VTIVCLDLGY TLECHNQQSS QTPTTTGCSG GETNCYKKRW
60 70 80
RDHRGYRTER GCGCPSVKNG IEINCCTTDR CNN
Length:83
Mass (Da):9,262
Last modified:April 13, 2004 - v1
Checksum:i4DD6077C92717052
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21K → E in AAG43384 (Ref. 4) Curated
Sequence conflicti52 – 521D → Y in CAB43097 (PubMed:10512733).Curated
Sequence conflicti66 – 661S → I in AAG43384 (Ref. 4) Curated
Sequence conflicti73 – 731I → S in AAG43384 (Ref. 4) Curated
Sequence conflicti79 – 802DR → G in CAB43097 (PubMed:10512733).Curated

Mass spectrometryi

Molecular mass is 6952 Da from positions 22 - 83. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58519 mRNA. Translation: AAB03221.1.
U58520 mRNA. Translation: AAB03222.1.
U58521 mRNA. Translation: AAB03223.1.
U77490 mRNA. Translation: AAB36930.1.
U77491 mRNA. Translation: AAB36931.1.
U77492 mRNA. Translation: AAB36932.1.
U42582 mRNA. Translation: AAB01538.1.
Y12492 Genomic DNA. Translation: CAA73097.2.
AF161797 Genomic DNA. Translation: AAG43384.1.
AJ239050 mRNA. Translation: CAB43097.1.
AY471578 mRNA. Translation: AAR33035.1.
PIRiJC5769. N1NJ1F.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58519 mRNA. Translation: AAB03221.1.
U58520 mRNA. Translation: AAB03222.1.
U58521 mRNA. Translation: AAB03223.1.
U77490 mRNA. Translation: AAB36930.1.
U77491 mRNA. Translation: AAB36931.1.
U77492 mRNA. Translation: AAB36932.1.
U42582 mRNA. Translation: AAB01538.1.
Y12492 Genomic DNA. Translation: CAA73097.2.
AF161797 Genomic DNA. Translation: AAG43384.1.
AJ239050 mRNA. Translation: CAB43097.1.
AY471578 mRNA. Translation: AAR33035.1.
PIRiJC5769. N1NJ1F.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1COENMR-A22-83[»]
1V6PX-ray0.87A/B22-83[»]
ProteinModelPortaliP60770.
SMRiP60770. Positions 22-83.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG006553.

Miscellaneous databases

EvolutionaryTraceiP60770.

Family and domain databases

CDDicd00206. snake_toxin. 1 hit.
InterProiIPR003571. Snake_3FTx.
IPR018354. Snake_toxin_con_site.
[Graphical view]
PROSITEiPS00272. SNAKE_TOXIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei3S1CB_NAJAT
AccessioniPrimary (citable) accession number: P60770
Secondary accession number(s): O13079
, P01430, Q675L7, Q9DE57, Q9W6X0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 13, 2004
Last modified: September 7, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.