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P60763 (RAC3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras-related C3 botulinum toxin substrate 3
Alternative name(s):
p21-Rac3
Gene names
Name:RAC3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length192 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Promotes cell adhesion and spreading on fibrinogen in a CIB1 and alpha-IIb/beta3 integrin-mediated manner. Ref.6 Ref.7

Subunit structure

Interacts with the GEF protein DOCK7, which promotes the exchange between GDP and GTP, and therefore activates it. Interacts with C1D. Interacts (via C-terminus region) with CIB1; the interaction induces their association with the cytoskeleton upon alpha-IIb/beta3 integrin-mediated adhesion. Ref.5 Ref.6 Ref.7 Ref.8

Subcellular location

Cytoplasm. Endomembrane system. Cell projectionlamellipodium. Cytoplasmperinuclear region. Cell membrane. Cytoplasmcytoskeleton. Note: Membrane-associated when activated. Colocalizes with NRBP to endomembranes and at the cell periphery in lamellipodia. Colocalized with CIB1 in the perinuclear area and at the cell periphery. Ref.6 Ref.7

Tissue specificity

Highest levels in brain, also detected in heart, placenta and pancreas.

Induction

Expression down-regulated in quiescent fibroblasts and clearly induced by serum stimulation.

Sequence similarities

Belongs to the small GTPase superfamily. Rho family.

Ontologies

Keywords
   Cellular componentCell membrane
Cell projection
Cytoplasm
Cytoskeleton
Membrane
   LigandGTP-binding
Nucleotide-binding
   PTMLipoprotein
Methylation
Prenylation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processGTP catabolic process

Inferred from direct assay Ref.6. Source: GOC

actin cytoskeleton organization

Inferred from direct assay Ref.6. Source: UniProtKB

cell projection assembly

Inferred from direct assay Ref.6. Source: UniProtKB

intracellular signal transduction

Traceable author statement Ref.1. Source: UniProtKB

neuromuscular process controlling balance

Inferred from electronic annotation. Source: Ensembl

neuron projection development

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell adhesion mediated by integrin

Inferred from direct assay Ref.7. Source: UniProtKB

positive regulation of substrate adhesion-dependent cell spreading

Inferred from direct assay Ref.7. Source: UniProtKB

regulation of small GTPase mediated signal transduction

Traceable author statement. Source: Reactome

small GTPase mediated signal transduction

Traceable author statement. Source: Reactome

   Cellular_componentcell periphery

Inferred from direct assay Ref.7. Source: UniProtKB

cytosol

Traceable author statement. Source: Reactome

endomembrane system

Inferred from direct assay Ref.6. Source: UniProtKB

filamentous actin

Inferred from direct assay PubMed 16525025. Source: MGI

growth cone

Inferred from direct assay PubMed 16525025. Source: MGI

lamellipodium

Inferred from electronic annotation. Source: UniProtKB-SubCell

neuron projection

Inferred from direct assay PubMed 16525025. Source: MGI

neuronal cell body

Inferred from direct assay PubMed 16525025. Source: MGI

perinuclear region of cytoplasm

Inferred from direct assay Ref.7. Source: UniProtKB

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from direct assay Ref.6. Source: UniProtKB

calcium-dependent protein binding

Inferred from physical interaction Ref.7. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ESR1P033725EBI-767084,EBI-78473
NRBP1Q9UHY13EBI-767084,EBI-749731

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 189189Ras-related C3 botulinum toxin substrate 3
PRO_0000198889
Propeptide190 – 1923Removed in mature form By similarity
PRO_0000281240

Regions

Nucleotide binding10 – 178GTP By similarity
Nucleotide binding57 – 615GTP By similarity
Nucleotide binding115 – 1184GTP By similarity
Motif32 – 409Effector region Potential

Amino acid modifications

Modified residue1891Cysteine methyl ester By similarity
Lipidation1891S-geranylgeranyl cysteine By similarity

Secondary structure

................................... 192
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P60763 [UniParc].

Last modified April 13, 2004. Version 1.
Checksum: 560BBC26BB7CDF4A

FASTA19221,379
        10         20         30         40         50         60 
MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGKP VNLGLWDTAG 

        70         80         90        100        110        120 
QEDYDRLRPL SYPQTDVFLI CFSLVSPASF ENVRAKWYPE VRHHCPHTPI LLVGTKLDLR 

       130        140        150        160        170        180 
DDKDTIERLR DKKLAPITYP QGLAMAREIG SVKYLECSAL TQRGLKTVFD EAIRAVLCPP 

       190 
PVKKPGKKCT VF 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of RAC3, a novel member of the Rho family."
Haataja L., Groffen J., Heisterkamp N.
J. Biol. Chem. 272:20384-20388(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Puhl H.L. III, Ikeda S.R., Aronstam R.S.
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[5]"Identification of a novel Rac3-interacting protein C1D."
Haataja L., Groffen J., Heisterkamp N.
Int. J. Mol. Med. 1:665-670(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH C1D.
[6]"Interaction of the small GTPase Rac3 with NRBP, a protein with a kinase-homology domain."
De Langhe S., Haataja L., Senadheera D., Groffen J., Heisterkamp N.
Int. J. Mol. Med. 9:451-459(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH NRBP.
[7]"The small GTPase Rac3 interacts with the integrin-binding protein CIB and promotes integrin alpha(IIb)beta(3)-mediated adhesion and spreading."
Haataja L., Kaartinen V., Groffen J., Heisterkamp N.
J. Biol. Chem. 277:8321-8328(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CIB1, SUBCELLULAR LOCATION.
[8]"The Rac activator DOCK7 regulates neuronal polarity through local phosphorylation of stathmin/Op18."
Watabe-Uchida M., John K.A., Janas J.A., Newey S.E., Van Aelst L.
Neuron 51:727-739(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DOCK7.
[9]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF008591 mRNA. Translation: AAC51667.1.
AF498966 mRNA. Translation: AAM21113.1.
BT019443 mRNA. Translation: AAV38250.1.
BC009605 mRNA. Translation: AAH09605.1.
BC015197 mRNA. Translation: AAH15197.1.
RefSeqNP_005043.1. NM_005052.2.
UniGeneHs.45002.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2C2HX-ray1.85A/B1-192[»]
2G0NX-ray1.90A/B1-177[»]
2IC5X-ray1.90A/B1-177[»]
2OV2X-ray2.10A/B/C/D/E/F/G/H1-177[»]
2QMEX-ray1.75A1-177[»]
ProteinModelPortalP60763.
SMRP60763. Positions 1-177.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111819. 10 interactions.
DIPDIP-33881N.
IntActP60763. 21 interactions.
MINTMINT-1613852.
STRING9606.ENSP00000304283.

Chemistry

ChEMBLCHEMBL6087.

PTM databases

PhosphoSiteP60763.

Polymorphism databases

DMDM46397673.

Proteomic databases

PaxDbP60763.
PRIDEP60763.

Protocols and materials databases

DNASU5881.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000306897; ENSP00000304283; ENSG00000169750.
GeneID5881.
KEGGhsa:5881.
UCSCuc002kdf.3. human.

Organism-specific databases

CTD5881.
GeneCardsGC17P079989.
HGNCHGNC:9803. RAC3.
MIM602050. gene.
neXtProtNX_P60763.
PharmGKBPA34164.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1100.
HOGENOMHOG000233974.
HOVERGENHBG009351.
InParanoidP60763.
KOK07861.
OMAPPIKKPG.
OrthoDBEOG764747.
PhylomeDBP60763.
TreeFamTF101109.

Enzyme and pathway databases

BRENDA3.6.5.2. 2681.
ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressP60763.
BgeeP60763.
CleanExHS_RAC3.
GenevestigatorP60763.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00174. RHO. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51420. RHO. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP60763.
GeneWikiRAC3.
GenomeRNAi5881.
NextBio22858.
PROP60763.
SOURCESearch...

Entry information

Entry nameRAC3_HUMAN
AccessionPrimary (citable) accession number: P60763
Secondary accession number(s): O14658, Q5U0M8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM