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Protein

Ras-related C3 botulinum toxin substrate 3

Gene

RAC3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Promotes cell adhesion and spreading on fibrinogen in a CIB1 and alpha-IIb/beta3 integrin-mediated manner.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 17GTPBy similarity8
Nucleotide bindingi57 – 61GTPBy similarity5
Nucleotide bindingi115 – 118GTPBy similarity4

GO - Molecular functioni

  • calcium-dependent protein binding Source: UniProtKB
  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000169750-MONOMER.
BRENDAi3.6.5.2. 2681.
ReactomeiR-HSA-194840. Rho GTPase cycle.
R-HSA-4086400. PCP/CE pathway.
SIGNORiP60763.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related C3 botulinum toxin substrate 3
Alternative name(s):
p21-Rac3
Gene namesi
Name:RAC3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:9803. RAC3.

Subcellular locationi

GO - Cellular componenti

  • cell periphery Source: UniProtKB
  • cytosol Source: Reactome
  • endomembrane system Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • filamentous actin Source: MGI
  • growth cone Source: MGI
  • lamellipodium Source: UniProtKB-SubCell
  • neuronal cell body Source: MGI
  • neuron projection Source: MGI
  • perinuclear region of cytoplasm Source: UniProtKB
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi5881.
OpenTargetsiENSG00000169750.
PharmGKBiPA34164.

Chemistry databases

ChEMBLiCHEMBL6087.

Polymorphism and mutation databases

BioMutaiRAC3.
DMDMi46397673.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001988891 – 189Ras-related C3 botulinum toxin substrate 3Add BLAST189
PropeptideiPRO_0000281240190 – 192Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi32O-linked (GlcNAc); by Photorhabdus PAU_022301 Publication1
Modified residuei189Cysteine methyl esterBy similarity1
Lipidationi189S-geranylgeranyl cysteineBy similarity1

Post-translational modificationi

(Microbial infection) Glycosylated at Tyr-32 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rac and leads to actin disassembly.1 Publication

Keywords - PTMi

Glycoprotein, Lipoprotein, Methylation, Prenylation

Proteomic databases

EPDiP60763.
MaxQBiP60763.
PaxDbiP60763.
PeptideAtlasiP60763.
PRIDEiP60763.
TopDownProteomicsiP60763.

PTM databases

iPTMnetiP60763.
PhosphoSitePlusiP60763.
SwissPalmiP60763.

Expressioni

Tissue specificityi

Highest levels in brain, also detected in heart, placenta and pancreas.

Inductioni

Expression down-regulated in quiescent fibroblasts and clearly induced by serum stimulation.

Gene expression databases

BgeeiENSG00000169750.
CleanExiHS_RAC3.
ExpressionAtlasiP60763. baseline and differential.
GenevisibleiP60763. HS.

Organism-specific databases

HPAiHPA047820.

Interactioni

Subunit structurei

Interacts with the GEF protein DOCK7, which promotes the exchange between GDP and GTP, and therefore activates it. Interacts with C1D. Interacts (via C-terminal region) with CIB1; the interaction induces their association with the cytoskeleton upon alpha-IIb/beta3 integrin-mediated adhesion.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ESR1P033725EBI-767084,EBI-78473
NRBP1Q9UHY13EBI-767084,EBI-749731

GO - Molecular functioni

  • calcium-dependent protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi111819. 11 interactors.
DIPiDIP-33881N.
IntActiP60763. 21 interactors.
MINTiMINT-1613852.
STRINGi9606.ENSP00000304283.

Structurei

Secondary structure

1192
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 10Combined sources9
Helixi16 – 25Combined sources10
Beta strandi36 – 46Combined sources11
Beta strandi49 – 58Combined sources10
Helixi62 – 64Combined sources3
Turni65 – 67Combined sources3
Helixi68 – 71Combined sources4
Beta strandi76 – 83Combined sources8
Helixi87 – 95Combined sources9
Helixi97 – 104Combined sources8
Beta strandi105 – 108Combined sources4
Beta strandi110 – 115Combined sources6
Helixi117 – 119Combined sources3
Helixi123 – 130Combined sources8
Turni131 – 133Combined sources3
Helixi139 – 148Combined sources10
Beta strandi152 – 156Combined sources5
Turni159 – 161Combined sources3
Helixi165 – 177Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2C2HX-ray1.85A/B1-192[»]
2G0NX-ray1.90A/B1-177[»]
2IC5X-ray1.90A/B1-178[»]
2OV2X-ray2.10A/B/C/D/E/F/G/H1-177[»]
2QMEX-ray1.75A1-177[»]
ProteinModelPortaliP60763.
SMRiP60763.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60763.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 40Effector regionSequence analysis9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiKOG0393. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000118978.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiP60763.
KOiK07861.
OMAiGKKCTMF.
OrthoDBiEOG091G0KCM.
PhylomeDBiP60763.
TreeFamiTF101109.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60763-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGKP
60 70 80 90 100
VNLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSLVSPASF ENVRAKWYPE
110 120 130 140 150
VRHHCPHTPI LLVGTKLDLR DDKDTIERLR DKKLAPITYP QGLAMAREIG
160 170 180 190
SVKYLECSAL TQRGLKTVFD EAIRAVLCPP PVKKPGKKCT VF
Length:192
Mass (Da):21,379
Last modified:April 13, 2004 - v1
Checksum:i560BBC26BB7CDF4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008591 mRNA. Translation: AAC51667.1.
AF498966 mRNA. Translation: AAM21113.1.
BT019443 mRNA. Translation: AAV38250.1.
BC009605 mRNA. Translation: AAH09605.1.
BC015197 mRNA. Translation: AAH15197.1.
CCDSiCCDS11798.1.
RefSeqiNP_005043.1. NM_005052.2.
UniGeneiHs.45002.

Genome annotation databases

EnsembliENST00000306897; ENSP00000304283; ENSG00000169750.
GeneIDi5881.
KEGGihsa:5881.
UCSCiuc002kdf.4. human.

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008591 mRNA. Translation: AAC51667.1.
AF498966 mRNA. Translation: AAM21113.1.
BT019443 mRNA. Translation: AAV38250.1.
BC009605 mRNA. Translation: AAH09605.1.
BC015197 mRNA. Translation: AAH15197.1.
CCDSiCCDS11798.1.
RefSeqiNP_005043.1. NM_005052.2.
UniGeneiHs.45002.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2C2HX-ray1.85A/B1-192[»]
2G0NX-ray1.90A/B1-177[»]
2IC5X-ray1.90A/B1-178[»]
2OV2X-ray2.10A/B/C/D/E/F/G/H1-177[»]
2QMEX-ray1.75A1-177[»]
ProteinModelPortaliP60763.
SMRiP60763.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111819. 11 interactors.
DIPiDIP-33881N.
IntActiP60763. 21 interactors.
MINTiMINT-1613852.
STRINGi9606.ENSP00000304283.

Chemistry databases

ChEMBLiCHEMBL6087.

PTM databases

iPTMnetiP60763.
PhosphoSitePlusiP60763.
SwissPalmiP60763.

Polymorphism and mutation databases

BioMutaiRAC3.
DMDMi46397673.

Proteomic databases

EPDiP60763.
MaxQBiP60763.
PaxDbiP60763.
PeptideAtlasiP60763.
PRIDEiP60763.
TopDownProteomicsiP60763.

Protocols and materials databases

DNASUi5881.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000306897; ENSP00000304283; ENSG00000169750.
GeneIDi5881.
KEGGihsa:5881.
UCSCiuc002kdf.4. human.

Organism-specific databases

CTDi5881.
DisGeNETi5881.
GeneCardsiRAC3.
HGNCiHGNC:9803. RAC3.
HPAiHPA047820.
MIMi602050. gene.
neXtProtiNX_P60763.
OpenTargetsiENSG00000169750.
PharmGKBiPA34164.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0393. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000118978.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiP60763.
KOiK07861.
OMAiGKKCTMF.
OrthoDBiEOG091G0KCM.
PhylomeDBiP60763.
TreeFamiTF101109.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000169750-MONOMER.
BRENDAi3.6.5.2. 2681.
ReactomeiR-HSA-194840. Rho GTPase cycle.
R-HSA-4086400. PCP/CE pathway.
SIGNORiP60763.

Miscellaneous databases

EvolutionaryTraceiP60763.
GeneWikiiRAC3.
GenomeRNAii5881.
PROiP60763.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000169750.
CleanExiHS_RAC3.
ExpressionAtlasiP60763. baseline and differential.
GenevisibleiP60763. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAC3_HUMAN
AccessioniPrimary (citable) accession number: P60763
Secondary accession number(s): O14658, Q5U0M8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: November 2, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.