Ras-related C3 botulinum toxin substrate 3 precursor

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Reviewed, UniProtKB/Swiss-Prot P60763 (RAC3_HUMAN)

Last modified June 16, 2009. Version 64. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Ras-related C3 botulinum toxin substrate 3
Alternative name(s):
p21-Rac3

Gene names

Name:

RAC3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Ref.6
Subunit structure	Interacts with the GEF protein DOCK7, which promotes the exchange between GDP and GTP, and therefore activates it. Interacts with C1D. Ref.6 Ref.5 Ref.7
Subcellular location	Cytoplasm. Endomembrane system. Cell projection › lamellipodium. Note: Membrane-associated when activated. Co-localizes with NRBP to endomembranes and at the cell periphery in lamellipodia.
Tissue specificity	Highest levels in brain, also detected in heart, placenta and pancreas.
Induction	Expression down-regulated in quiescent fibroblasts and clearly induced by serum stimulation.
Sequence similarities	Belongs to the small GTPase superfamily. Rho family.

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Ontologies

Keywords
Cellular component	Cell projection Cytoplasm Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Phosphoprotein Prenylation
Technical term	3D-structure
Gene Ontology (GO)
Biological process	actin cytoskeleton organization Ref.6 Inferred from direct assay. Source: UniProtKB cell projection assembly Ref.6 Inferred from direct assay. Source: UniProtKB small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cell soma Inferred from direct assay. Source: MGI cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell endomembrane system Ref.6 Inferred from direct assay. Source: UniProtKB filamentous actin Inferred from direct assay. Source: MGI growth cone Inferred from direct assay. Source: MGI lamellipodium Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-KW neuron projection Inferred from direct assay. Source: MGI
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Ref.6 Inferred from direct assay. Source: UniProtKB protein binding Ref.6 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
NRBP1	Q9UHY1	1	EBI-767084,EBI-749731

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 189

189

Ras-related C3 botulinum toxin substrate 3

PRO_0000198889

Propeptide

190 – 192

Removed in mature form By similarity

PRO_0000281240

Regions

Nucleotide binding

10 – 17

GTP By similarity

Nucleotide binding

57 – 61

GTP By similarity

Nucleotide binding

115 – 118

GTP By similarity

Motif

32 – 40

Effector region Potential

Amino acid modifications

Modified residue

167

Phosphothreonine Ref.8

Modified residue

189

Cysteine methyl ester By similarity

Lipidation

189

S-geranylgeranyl cysteine By similarity

Secondary structure

192

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P60763-1 [UniParc].

Last modified April 13, 2004. Version 1.
Checksum: 560BBC26BB7CDF4A
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FASTA

192

21,379

        10         20         30         40         50         60 
MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGKP VNLGLWDTAG 

        70         80         90        100        110        120 
QEDYDRLRPL SYPQTDVFLI CFSLVSPASF ENVRAKWYPE VRHHCPHTPI LLVGTKLDLR 

       130        140        150        160        170        180 
DDKDTIERLR DKKLAPITYP QGLAMAREIG SVKYLECSAL TQRGLKTVFD EAIRAVLCPP 

       190 
PVKKPGKKCT VF

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of RAC3, a novel member of the Rho family." Haataja L., Groffen J., Heisterkamp N. J. Biol. Chem. 272:20384-20388(1997) [PubMed: 9252344] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Identification of a novel Rac3-interacting protein C1D." Haataja L., Groffen J., Heisterkamp N. Int. J. Mol. Med. 1:665-670(1998) [PubMed: 9852280] [Abstract] Cited for: INTERACTION WITH C1D.
[6]	"Interaction of the small GTPase Rac3 with NRBP, a protein with a kinase-homology domain." De Langhe S., Haataja L., Senadheera D., Groffen J., Heisterkamp N. Int. J. Mol. Med. 9:451-459(2002) [PubMed: 11956649] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH NRBP.
[7]	"The Rac activator DOCK7 regulates neuronal polarity through local phosphorylation of stathmin/Op18." Watabe-Uchida M., John K.A., Janas J.A., Newey S.E., Van Aelst L. Neuron 51:727-739(2006) [PubMed: 16982419] [Abstract] Cited for: INTERACTION WITH DOCK7.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-167, MASS SPECTROMETRY.
[9]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

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Cross-references

Sequence databases

AF008591 mRNA. Translation: AAC51667.1.
AF498966 mRNA. Translation: AAM21113.1.
BT019443 mRNA. Translation: AAV38250.1.
BC009605 mRNA. Translation: AAH09605.1.
BC015197 mRNA. Translation: AAH15197.1.

IPI

IPI00023138.

RefSeq

NP_005043.1.

UniGene

Hs.45002

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2C2H	X-ray	1.85	A/B	1-192	[»]
2G0N	X-ray	1.90	A/B	1-177	[»]
2IC5	X-ray	1.90	A/B	1-177	[»]
2OV2	X-ray	2.10	A/B/C/D/E/F/G/H	1-177	[»]
2QME	X-ray	1.75	A	1-177	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P60763. 1 interaction.

Proteomic databases

PRIDE

P60763.

Genome annotation databases

Ensembl

ENSG00000169750. Homo sapiens. [Contig view]

GeneID

5881.

KEGG

hsa:5881.

Organism-specific databases

GeneCards

GC17P077582.

H-InvDB

HIX0014265.

HGNC

HGNC:9803. RAC3.

MIM

602050. gene.

PharmGKB

PA34164.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P60763.

HOVERGEN

P60763.

OMA

P60763. TQVGVKE.

Enzyme and pathway databases

BRENDA

3.6.5.2. 247.

Reactome

REACT_11044. Signaling by Rho GTPases.

Gene expression databases

Bgee

P60763.

CleanEx

HS_RAC3.

GermOnline

ENSG00000169750. Homo sapiens.

Family and domain databases

InterPro

IPR003578. GTPase_Rho.
IPR013753. Ras.
IPR001806. Ras_GTPase.
IPR005225. Small_GTP_bd.
[Graphical view]

Pfam

PF00071. Ras. 1 hit.
[Graphical view]

PRINTS

PR00449. RASTRNSFRMNG.

SMART

SM00174. RHO. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. small_GTP. 1 hit.

PROSITE

PS51420. RHO. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

22858.

SOURCE

Search...

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Entry information

Entry name

RAC3_HUMAN

Accession

Primary (citable) accession number: P60763
Secondary accession number(s): O14658, Q5U0M8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	April 13, 2004
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Web resources

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents