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P60763

- RAC3_HUMAN

UniProt

P60763 - RAC3_HUMAN

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Protein
Ras-related C3 botulinum toxin substrate 3
Gene
RAC3
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Promotes cell adhesion and spreading on fibrinogen in a CIB1 and alpha-IIb/beta3 integrin-mediated manner.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 178GTP By similarity
Nucleotide bindingi57 – 615GTP By similarity
Nucleotide bindingi115 – 1184GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. GTPase activity Source: UniProtKB
  3. calcium-dependent protein binding Source: UniProtKB
  4. protein binding Source: IntAct

GO - Biological processi

  1. GTP catabolic process Source: GOC
  2. actin cytoskeleton organization Source: UniProtKB
  3. cell projection assembly Source: UniProtKB
  4. intracellular signal transduction Source: UniProtKB
  5. neuromuscular process controlling balance Source: Ensembl
  6. neuron projection development Source: Ensembl
  7. positive regulation of cell adhesion mediated by integrin Source: UniProtKB
  8. positive regulation of substrate adhesion-dependent cell spreading Source: UniProtKB
  9. regulation of small GTPase mediated signal transduction Source: Reactome
  10. small GTPase mediated signal transduction Source: Reactome
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.5.2. 2681.
ReactomeiREACT_11051. Rho GTPase cycle.
REACT_172581. PCP/CE pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related C3 botulinum toxin substrate 3
Alternative name(s):
p21-Rac3
Gene namesi
Name:RAC3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:9803. RAC3.

Subcellular locationi

Cytoplasm. Endomembrane system. Cell projectionlamellipodium. Cytoplasmperinuclear region. Cell membrane. Cytoplasmcytoskeleton
Note: Membrane-associated when activated. Colocalizes with NRBP to endomembranes and at the cell periphery in lamellipodia. Colocalized with CIB1 in the perinuclear area and at the cell periphery.2 Publications

GO - Cellular componenti

  1. cell periphery Source: UniProtKB
  2. cytosol Source: Reactome
  3. endomembrane system Source: UniProtKB
  4. filamentous actin Source: MGI
  5. growth cone Source: MGI
  6. lamellipodium Source: UniProtKB-SubCell
  7. neuron projection Source: MGI
  8. neuronal cell body Source: MGI
  9. perinuclear region of cytoplasm Source: UniProtKB
  10. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34164.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 189189Ras-related C3 botulinum toxin substrate 3
PRO_0000198889Add
BLAST
Propeptidei190 – 1923Removed in mature form By similarity
PRO_0000281240

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei189 – 1891Cysteine methyl ester By similarity
Lipidationi189 – 1891S-geranylgeranyl cysteine By similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

MaxQBiP60763.
PaxDbiP60763.
PRIDEiP60763.

PTM databases

PhosphoSiteiP60763.

Expressioni

Tissue specificityi

Highest levels in brain, also detected in heart, placenta and pancreas.

Inductioni

Expression down-regulated in quiescent fibroblasts and clearly induced by serum stimulation.

Gene expression databases

ArrayExpressiP60763.
BgeeiP60763.
CleanExiHS_RAC3.
GenevestigatoriP60763.

Interactioni

Subunit structurei

Interacts with the GEF protein DOCK7, which promotes the exchange between GDP and GTP, and therefore activates it. Interacts with C1D. Interacts (via C-terminal region) with CIB1; the interaction induces their association with the cytoskeleton upon alpha-IIb/beta3 integrin-mediated adhesion.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ESR1P033725EBI-767084,EBI-78473
NRBP1Q9UHY13EBI-767084,EBI-749731

Protein-protein interaction databases

BioGridi111819. 11 interactions.
DIPiDIP-33881N.
IntActiP60763. 21 interactions.
MINTiMINT-1613852.
STRINGi9606.ENSP00000304283.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 109
Helixi16 – 2510
Beta strandi36 – 4611
Beta strandi49 – 5810
Helixi62 – 643
Turni65 – 673
Helixi68 – 714
Beta strandi76 – 838
Helixi87 – 959
Helixi97 – 1048
Beta strandi105 – 1084
Beta strandi110 – 1156
Helixi117 – 1193
Helixi123 – 1308
Turni131 – 1333
Helixi139 – 14810
Beta strandi152 – 1565
Turni159 – 1613
Helixi165 – 17713

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2C2HX-ray1.85A/B1-192[»]
2G0NX-ray1.90A/B1-177[»]
2IC5X-ray1.90A/B1-178[»]
2OV2X-ray2.10A/B/C/D/E/F/G/H1-177[»]
2QMEX-ray1.75A1-177[»]
ProteinModelPortaliP60763.
SMRiP60763. Positions 1-177.

Miscellaneous databases

EvolutionaryTraceiP60763.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi32 – 409Effector region Reviewed prediction

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1100.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiP60763.
KOiK07861.
OMAiGKKCTMF.
OrthoDBiEOG764747.
PhylomeDBiP60763.
TreeFamiTF101109.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00174. RHO. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60763-1 [UniParc]FASTAAdd to Basket

« Hide

MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGKP    50
VNLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSLVSPASF ENVRAKWYPE 100
VRHHCPHTPI LLVGTKLDLR DDKDTIERLR DKKLAPITYP QGLAMAREIG 150
SVKYLECSAL TQRGLKTVFD EAIRAVLCPP PVKKPGKKCT VF 192
Length:192
Mass (Da):21,379
Last modified:April 13, 2004 - v1
Checksum:i560BBC26BB7CDF4A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF008591 mRNA. Translation: AAC51667.1.
AF498966 mRNA. Translation: AAM21113.1.
BT019443 mRNA. Translation: AAV38250.1.
BC009605 mRNA. Translation: AAH09605.1.
BC015197 mRNA. Translation: AAH15197.1.
CCDSiCCDS11798.1.
RefSeqiNP_005043.1. NM_005052.2.
UniGeneiHs.45002.

Genome annotation databases

EnsembliENST00000306897; ENSP00000304283; ENSG00000169750.
GeneIDi5881.
KEGGihsa:5881.
UCSCiuc002kdf.3. human.

Polymorphism databases

DMDMi46397673.

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF008591 mRNA. Translation: AAC51667.1 .
AF498966 mRNA. Translation: AAM21113.1 .
BT019443 mRNA. Translation: AAV38250.1 .
BC009605 mRNA. Translation: AAH09605.1 .
BC015197 mRNA. Translation: AAH15197.1 .
CCDSi CCDS11798.1.
RefSeqi NP_005043.1. NM_005052.2.
UniGenei Hs.45002.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2C2H X-ray 1.85 A/B 1-192 [» ]
2G0N X-ray 1.90 A/B 1-177 [» ]
2IC5 X-ray 1.90 A/B 1-178 [» ]
2OV2 X-ray 2.10 A/B/C/D/E/F/G/H 1-177 [» ]
2QME X-ray 1.75 A 1-177 [» ]
ProteinModelPortali P60763.
SMRi P60763. Positions 1-177.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 111819. 11 interactions.
DIPi DIP-33881N.
IntActi P60763. 21 interactions.
MINTi MINT-1613852.
STRINGi 9606.ENSP00000304283.

Chemistry

ChEMBLi CHEMBL6087.

PTM databases

PhosphoSitei P60763.

Polymorphism databases

DMDMi 46397673.

Proteomic databases

MaxQBi P60763.
PaxDbi P60763.
PRIDEi P60763.

Protocols and materials databases

DNASUi 5881.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000306897 ; ENSP00000304283 ; ENSG00000169750 .
GeneIDi 5881.
KEGGi hsa:5881.
UCSCi uc002kdf.3. human.

Organism-specific databases

CTDi 5881.
GeneCardsi GC17P079989.
HGNCi HGNC:9803. RAC3.
MIMi 602050. gene.
neXtProti NX_P60763.
PharmGKBi PA34164.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1100.
HOGENOMi HOG000233974.
HOVERGENi HBG009351.
InParanoidi P60763.
KOi K07861.
OMAi GKKCTMF.
OrthoDBi EOG764747.
PhylomeDBi P60763.
TreeFami TF101109.

Enzyme and pathway databases

BRENDAi 3.6.5.2. 2681.
Reactomei REACT_11051. Rho GTPase cycle.
REACT_172581. PCP/CE pathway.

Miscellaneous databases

EvolutionaryTracei P60763.
GeneWikii RAC3.
GenomeRNAii 5881.
NextBioi 22858.
PROi P60763.
SOURCEi Search...

Gene expression databases

ArrayExpressi P60763.
Bgeei P60763.
CleanExi HS_RAC3.
Genevestigatori P60763.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00174. RHO. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51420. RHO. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of RAC3, a novel member of the Rho family."
    Haataja L., Groffen J., Heisterkamp N.
    J. Biol. Chem. 272:20384-20388(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    Puhl H.L. III, Ikeda S.R., Aronstam R.S.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. "Identification of a novel Rac3-interacting protein C1D."
    Haataja L., Groffen J., Heisterkamp N.
    Int. J. Mol. Med. 1:665-670(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH C1D.
  6. "Interaction of the small GTPase Rac3 with NRBP, a protein with a kinase-homology domain."
    De Langhe S., Haataja L., Senadheera D., Groffen J., Heisterkamp N.
    Int. J. Mol. Med. 9:451-459(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH NRBP.
  7. "The small GTPase Rac3 interacts with the integrin-binding protein CIB and promotes integrin alpha(IIb)beta(3)-mediated adhesion and spreading."
    Haataja L., Kaartinen V., Groffen J., Heisterkamp N.
    J. Biol. Chem. 277:8321-8328(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CIB1, SUBCELLULAR LOCATION.
  8. "The Rac activator DOCK7 regulates neuronal polarity through local phosphorylation of stathmin/Op18."
    Watabe-Uchida M., John K.A., Janas J.A., Newey S.E., Van Aelst L.
    Neuron 51:727-739(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DOCK7.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRAC3_HUMAN
AccessioniPrimary (citable) accession number: P60763
Secondary accession number(s): O14658, Q5U0M8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: September 3, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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