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P60763

- RAC3_HUMAN

UniProt

P60763 - RAC3_HUMAN

Protein

Ras-related C3 botulinum toxin substrate 3

Gene

RAC3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (13 Apr 2004)
      Previous versions | rss
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    Functioni

    Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Promotes cell adhesion and spreading on fibrinogen in a CIB1 and alpha-IIb/beta3 integrin-mediated manner.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 178GTPBy similarity
    Nucleotide bindingi57 – 615GTPBy similarity
    Nucleotide bindingi115 – 1184GTPBy similarity

    GO - Molecular functioni

    1. calcium-dependent protein binding Source: UniProtKB
    2. GTPase activity Source: UniProtKB
    3. GTP binding Source: UniProtKB-KW
    4. protein binding Source: IntAct

    GO - Biological processi

    1. actin cytoskeleton organization Source: UniProtKB
    2. cell projection assembly Source: UniProtKB
    3. GTP catabolic process Source: GOC
    4. intracellular signal transduction Source: UniProtKB
    5. neuromuscular process controlling balance Source: Ensembl
    6. neuron projection development Source: Ensembl
    7. positive regulation of cell adhesion mediated by integrin Source: UniProtKB
    8. positive regulation of substrate adhesion-dependent cell spreading Source: UniProtKB
    9. regulation of small GTPase mediated signal transduction Source: Reactome
    10. small GTPase mediated signal transduction Source: Reactome

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi3.6.5.2. 2681.
    ReactomeiREACT_11051. Rho GTPase cycle.
    REACT_172581. PCP/CE pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras-related C3 botulinum toxin substrate 3
    Alternative name(s):
    p21-Rac3
    Gene namesi
    Name:RAC3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:9803. RAC3.

    Subcellular locationi

    Cytoplasm. Endomembrane system. Cell projectionlamellipodium. Cytoplasmperinuclear region. Cell membrane. Cytoplasmcytoskeleton
    Note: Membrane-associated when activated. Colocalizes with NRBP to endomembranes and at the cell periphery in lamellipodia. Colocalized with CIB1 in the perinuclear area and at the cell periphery.

    GO - Cellular componenti

    1. cell periphery Source: UniProtKB
    2. cytosol Source: Reactome
    3. endomembrane system Source: UniProtKB
    4. filamentous actin Source: MGI
    5. growth cone Source: MGI
    6. lamellipodium Source: UniProtKB-SubCell
    7. neuronal cell body Source: MGI
    8. neuron projection Source: MGI
    9. perinuclear region of cytoplasm Source: UniProtKB
    10. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA34164.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 189189Ras-related C3 botulinum toxin substrate 3PRO_0000198889Add
    BLAST
    Propeptidei190 – 1923Removed in mature formBy similarityPRO_0000281240

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei189 – 1891Cysteine methyl esterBy similarity
    Lipidationi189 – 1891S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Methylation, Prenylation

    Proteomic databases

    MaxQBiP60763.
    PaxDbiP60763.
    PRIDEiP60763.

    PTM databases

    PhosphoSiteiP60763.

    Expressioni

    Tissue specificityi

    Highest levels in brain, also detected in heart, placenta and pancreas.

    Inductioni

    Expression down-regulated in quiescent fibroblasts and clearly induced by serum stimulation.

    Gene expression databases

    ArrayExpressiP60763.
    BgeeiP60763.
    CleanExiHS_RAC3.
    GenevestigatoriP60763.

    Interactioni

    Subunit structurei

    Interacts with the GEF protein DOCK7, which promotes the exchange between GDP and GTP, and therefore activates it. Interacts with C1D. Interacts (via C-terminal region) with CIB1; the interaction induces their association with the cytoskeleton upon alpha-IIb/beta3 integrin-mediated adhesion.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ESR1P033725EBI-767084,EBI-78473
    NRBP1Q9UHY13EBI-767084,EBI-749731

    Protein-protein interaction databases

    BioGridi111819. 11 interactions.
    DIPiDIP-33881N.
    IntActiP60763. 21 interactions.
    MINTiMINT-1613852.
    STRINGi9606.ENSP00000304283.

    Structurei

    Secondary structure

    1
    192
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 109
    Helixi16 – 2510
    Beta strandi36 – 4611
    Beta strandi49 – 5810
    Helixi62 – 643
    Turni65 – 673
    Helixi68 – 714
    Beta strandi76 – 838
    Helixi87 – 959
    Helixi97 – 1048
    Beta strandi105 – 1084
    Beta strandi110 – 1156
    Helixi117 – 1193
    Helixi123 – 1308
    Turni131 – 1333
    Helixi139 – 14810
    Beta strandi152 – 1565
    Turni159 – 1613
    Helixi165 – 17713

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2C2HX-ray1.85A/B1-192[»]
    2G0NX-ray1.90A/B1-177[»]
    2IC5X-ray1.90A/B1-178[»]
    2OV2X-ray2.10A/B/C/D/E/F/G/H1-177[»]
    2QMEX-ray1.75A1-177[»]
    ProteinModelPortaliP60763.
    SMRiP60763. Positions 1-177.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP60763.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi32 – 409Effector regionSequence Analysis

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rho family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    HOGENOMiHOG000233974.
    HOVERGENiHBG009351.
    InParanoidiP60763.
    KOiK07861.
    OMAiGKKCTMF.
    OrthoDBiEOG764747.
    PhylomeDBiP60763.
    TreeFamiTF101109.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003578. Small_GTPase_Rho.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00174. RHO. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51420. RHO. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P60763-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGKP    50
    VNLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSLVSPASF ENVRAKWYPE 100
    VRHHCPHTPI LLVGTKLDLR DDKDTIERLR DKKLAPITYP QGLAMAREIG 150
    SVKYLECSAL TQRGLKTVFD EAIRAVLCPP PVKKPGKKCT VF 192
    Length:192
    Mass (Da):21,379
    Last modified:April 13, 2004 - v1
    Checksum:i560BBC26BB7CDF4A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF008591 mRNA. Translation: AAC51667.1.
    AF498966 mRNA. Translation: AAM21113.1.
    BT019443 mRNA. Translation: AAV38250.1.
    BC009605 mRNA. Translation: AAH09605.1.
    BC015197 mRNA. Translation: AAH15197.1.
    CCDSiCCDS11798.1.
    RefSeqiNP_005043.1. NM_005052.2.
    UniGeneiHs.45002.

    Genome annotation databases

    EnsembliENST00000306897; ENSP00000304283; ENSG00000169750.
    GeneIDi5881.
    KEGGihsa:5881.
    UCSCiuc002kdf.3. human.

    Polymorphism databases

    DMDMi46397673.

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF008591 mRNA. Translation: AAC51667.1 .
    AF498966 mRNA. Translation: AAM21113.1 .
    BT019443 mRNA. Translation: AAV38250.1 .
    BC009605 mRNA. Translation: AAH09605.1 .
    BC015197 mRNA. Translation: AAH15197.1 .
    CCDSi CCDS11798.1.
    RefSeqi NP_005043.1. NM_005052.2.
    UniGenei Hs.45002.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2C2H X-ray 1.85 A/B 1-192 [» ]
    2G0N X-ray 1.90 A/B 1-177 [» ]
    2IC5 X-ray 1.90 A/B 1-178 [» ]
    2OV2 X-ray 2.10 A/B/C/D/E/F/G/H 1-177 [» ]
    2QME X-ray 1.75 A 1-177 [» ]
    ProteinModelPortali P60763.
    SMRi P60763. Positions 1-177.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111819. 11 interactions.
    DIPi DIP-33881N.
    IntActi P60763. 21 interactions.
    MINTi MINT-1613852.
    STRINGi 9606.ENSP00000304283.

    Chemistry

    ChEMBLi CHEMBL6087.

    PTM databases

    PhosphoSitei P60763.

    Polymorphism databases

    DMDMi 46397673.

    Proteomic databases

    MaxQBi P60763.
    PaxDbi P60763.
    PRIDEi P60763.

    Protocols and materials databases

    DNASUi 5881.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000306897 ; ENSP00000304283 ; ENSG00000169750 .
    GeneIDi 5881.
    KEGGi hsa:5881.
    UCSCi uc002kdf.3. human.

    Organism-specific databases

    CTDi 5881.
    GeneCardsi GC17P079989.
    HGNCi HGNC:9803. RAC3.
    MIMi 602050. gene.
    neXtProti NX_P60763.
    PharmGKBi PA34164.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1100.
    HOGENOMi HOG000233974.
    HOVERGENi HBG009351.
    InParanoidi P60763.
    KOi K07861.
    OMAi GKKCTMF.
    OrthoDBi EOG764747.
    PhylomeDBi P60763.
    TreeFami TF101109.

    Enzyme and pathway databases

    BRENDAi 3.6.5.2. 2681.
    Reactomei REACT_11051. Rho GTPase cycle.
    REACT_172581. PCP/CE pathway.

    Miscellaneous databases

    EvolutionaryTracei P60763.
    GeneWikii RAC3.
    GenomeRNAii 5881.
    NextBioi 22858.
    PROi P60763.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P60763.
    Bgeei P60763.
    CleanExi HS_RAC3.
    Genevestigatori P60763.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003578. Small_GTPase_Rho.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00174. RHO. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51420. RHO. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of RAC3, a novel member of the Rho family."
      Haataja L., Groffen J., Heisterkamp N.
      J. Biol. Chem. 272:20384-20388(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Puhl H.L. III, Ikeda S.R., Aronstam R.S.
      Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    5. "Identification of a novel Rac3-interacting protein C1D."
      Haataja L., Groffen J., Heisterkamp N.
      Int. J. Mol. Med. 1:665-670(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH C1D.
    6. "Interaction of the small GTPase Rac3 with NRBP, a protein with a kinase-homology domain."
      De Langhe S., Haataja L., Senadheera D., Groffen J., Heisterkamp N.
      Int. J. Mol. Med. 9:451-459(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH NRBP.
    7. "The small GTPase Rac3 interacts with the integrin-binding protein CIB and promotes integrin alpha(IIb)beta(3)-mediated adhesion and spreading."
      Haataja L., Kaartinen V., Groffen J., Heisterkamp N.
      J. Biol. Chem. 277:8321-8328(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CIB1, SUBCELLULAR LOCATION.
    8. "The Rac activator DOCK7 regulates neuronal polarity through local phosphorylation of stathmin/Op18."
      Watabe-Uchida M., John K.A., Janas J.A., Newey S.E., Van Aelst L.
      Neuron 51:727-739(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DOCK7.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRAC3_HUMAN
    AccessioniPrimary (citable) accession number: P60763
    Secondary accession number(s): O14658, Q5U0M8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: April 13, 2004
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3