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Protein

Mortality factor 4-like protein 1

Gene

Morf4l1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Also component of the mSin3A complex which acts to repress transcription by deacetylation of nucleosomal histones. Required for homologous recombination repair (HRR) and resistance to mitomycin C (MMC). Involved in the localization of PALB2, BRCA2 and RAD51, but not BRCA1, to DNA-damage foci (By similarity).By similarity

GO - Molecular functioni

  • chromatin binding Source: MGI
  • protein N-terminus binding Source: MGI

GO - Biological processi

  • cell proliferation Source: MGI
  • chromatin remodeling Source: MGI
  • chromatin silencing Source: GO_Central
  • double-strand break repair via homologous recombination Source: UniProtKB
  • histone deacetylation Source: UniProtKB
  • histone H2A acetylation Source: UniProtKB
  • histone H4 acetylation Source: UniProtKB
  • regulation of growth Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, Growth regulation, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Mortality factor 4-like protein 1
Alternative name(s):
MORF-related gene 15 protein
Testis-expressed gene 189 protein
Transcription factor-like protein MRG15
Gene namesi
Name:Morf4l1
Synonyms:Mrg15, Tex189
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1096551. Morf4l1.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000887651 – 362Mortality factor 4-like protein 1Add BLAST362

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei143N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP60762.
PaxDbiP60762.
PeptideAtlasiP60762.
PRIDEiP60762.

PTM databases

iPTMnetiP60762.
PhosphoSitePlusiP60762.

Expressioni

Gene expression databases

BgeeiENSMUSG00000062270.
CleanExiMM_MORF4L1.
ExpressionAtlasiP60762. baseline and differential.
GenevisibleiP60762. MM.

Interactioni

Subunit structurei

Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC. MORF4L1 may also participate in the formation of NuA4 related complexes which lack the KAT5/TIP60 catalytic subunit, but which include the SWI/SNF related protein SRCAP. Component of the mSin3A histone deacetylase complex, which includes SIN3A, HDAC2, ARID4B, MORF4L1, RBBP4/RbAp48, and RBBP7/RbAp46. Interacts with RB1 and KAT8. May also interact with PHF12 and one or more as yet undefined members of the TLE (transducin-like enhancer of split) family of transcriptional repressors. Interacts with the N-terminus of MRFAP1 (By similarity). Found in a complex composed of MORF4L1, MRFAP1 and RB1. Interacts with the entire BRCA complex, which contains BRCA1, PALB2, BRCA2 and RAD51. Interacts with PALB2 (By similarity). Forms a complex with MSL1 and NUPR1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Kdm5bQ80Y844EBI-2943018,EBI-1249551

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204126. 15 interactors.
IntActiP60762. 4 interactors.
MINTiMINT-4102211.
STRINGi10090.ENSMUSP00000082346.

Structurei

3D structure databases

ProteinModelPortaliP60762.
SMRiP60762.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini191 – 362MRGPROSITE-ProRule annotationAdd BLAST172

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni26 – 62Interaction with KAT8By similarityAdd BLAST37
Regioni133 – 266Sufficient for interaction with SIN3ABy similarityAdd BLAST134
Regioni164 – 230Interaction with RB1-1By similarityAdd BLAST67
Regioni188 – 342Sufficient for interaction with PHF12By similarityAdd BLAST155
Regioni323 – 344Interaction with RB1-2By similarityAdd BLAST22

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi135 – 146Nuclear localization signalSequence analysisAdd BLAST12

Sequence similaritiesi

Contains 1 MRG domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3001. Eukaryota.
ENOG410XR9F. LUCA.
GeneTreeiENSGT00530000063018.
HOGENOMiHOG000190863.
HOVERGENiHBG052487.
InParanoidiP60762.
KOiK11339.
OMAiTGTDANT.
OrthoDBiEOG091G0J32.
PhylomeDBiP60762.
TreeFamiTF323400.

Family and domain databases

InterProiIPR016197. Chromodomain-like.
IPR008676. MRG.
IPR026541. MRG_dom.
IPR025995. Tudor-knot.
[Graphical view]
PANTHERiPTHR10880. PTHR10880. 2 hits.
PfamiPF05712. MRG. 1 hit.
PF11717. Tudor-knot. 1 hit.
[Graphical view]
PIRSFiPIRSF038133. HAT_Nua4_EAF3/MRG15. 1 hit.
SUPFAMiSSF54160. SSF54160. 2 hits.
PROSITEiPS51640. MRG. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P60762-1) [UniParc]FASTAAdd to basket
Also known as: Mrg15-b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPKQDPKPK FQEGERVLCF HGPLLYEAKC VKVAIKDKQV KYFIHYSGWN
60 70 80 90 100
KKSAVRPRRS EKSLKTREDI VALFPVPEGA PSVHHPLLTS SWDEWVPESR
110 120 130 140 150
VLKYVDTNLQ KQRELQKANQ EQYAEGKMRG AAPGKKTSGL QQKNVEVKTK
160 170 180 190 200
KNKQKTPGNG DGGSTSETPQ PPRKKRARVD PTVENEETFM NRVEVKVKIP
210 220 230 240 250
EELKPWLVDD WDLITRQKQL FYLPAKKNVD SILEDYANYK KSRGNTDNKE
260 270 280 290 300
YAVNEVVAGI KEYFNVMLGT QLLYKFERPQ YAEILADHPD APMSQVYGAP
310 320 330 340 350
HLLRLFVRIG AMLAYTPLDE KSLALLLNYL HDFLKYLAKN SATLFSASDY
360
EVAPPEYHRK AV
Length:362
Mass (Da):41,493
Last modified:February 15, 2005 - v2
Checksum:iC044A3DC6A37BFE2
GO
Isoform 2 (identifier: P60762-2) [UniParc]FASTAAdd to basket
Also known as: Mrg15-a

The sequence of this isoform differs from the canonical sequence as follows:
     52-91: KSAVRPRRSEKSLKTREDIVALFPVPEGAPSVHHPLLTSS → N

Show »
Length:323
Mass (Da):37,231
Checksum:i33CDBEC7D9CF513F
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01289052 – 91KSAVR…LLTSS → N in isoform 2. 3 PublicationsAdd BLAST40

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF319620 mRNA. Translation: AAK07406.1.
AF319621 mRNA. Translation: AAK07407.1.
AK079115 mRNA. Translation: BAC37546.1.
AK016414 mRNA. Translation: BAB30219.1.
BC003894 mRNA. Translation: AAH03894.1.
BC017619 mRNA. Translation: AAH17619.1.
BC083118 mRNA. Translation: AAH83118.1.
BC085103 mRNA. Translation: AAH85103.1.
CCDSiCCDS23400.1. [P60762-1]
CCDS52885.1. [P60762-2]
RefSeqiNP_001034236.1. NM_001039147.2. [P60762-1]
NP_077751.1. NM_024431.3. [P60762-2]
UniGeneiMm.426209.
Mm.439775.

Genome annotation databases

EnsembliENSMUST00000085248; ENSMUSP00000082346; ENSMUSG00000062270. [P60762-1]
ENSMUST00000169860; ENSMUSP00000132020; ENSMUSG00000062270. [P60762-2]
GeneIDi21761.
KEGGimmu:21761.
UCSCiuc009qzw.2. mouse. [P60762-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF319620 mRNA. Translation: AAK07406.1.
AF319621 mRNA. Translation: AAK07407.1.
AK079115 mRNA. Translation: BAC37546.1.
AK016414 mRNA. Translation: BAB30219.1.
BC003894 mRNA. Translation: AAH03894.1.
BC017619 mRNA. Translation: AAH17619.1.
BC083118 mRNA. Translation: AAH83118.1.
BC085103 mRNA. Translation: AAH85103.1.
CCDSiCCDS23400.1. [P60762-1]
CCDS52885.1. [P60762-2]
RefSeqiNP_001034236.1. NM_001039147.2. [P60762-1]
NP_077751.1. NM_024431.3. [P60762-2]
UniGeneiMm.426209.
Mm.439775.

3D structure databases

ProteinModelPortaliP60762.
SMRiP60762.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204126. 15 interactors.
IntActiP60762. 4 interactors.
MINTiMINT-4102211.
STRINGi10090.ENSMUSP00000082346.

PTM databases

iPTMnetiP60762.
PhosphoSitePlusiP60762.

Proteomic databases

MaxQBiP60762.
PaxDbiP60762.
PeptideAtlasiP60762.
PRIDEiP60762.

Protocols and materials databases

DNASUi21761.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000085248; ENSMUSP00000082346; ENSMUSG00000062270. [P60762-1]
ENSMUST00000169860; ENSMUSP00000132020; ENSMUSG00000062270. [P60762-2]
GeneIDi21761.
KEGGimmu:21761.
UCSCiuc009qzw.2. mouse. [P60762-1]

Organism-specific databases

CTDi10933.
MGIiMGI:1096551. Morf4l1.

Phylogenomic databases

eggNOGiKOG3001. Eukaryota.
ENOG410XR9F. LUCA.
GeneTreeiENSGT00530000063018.
HOGENOMiHOG000190863.
HOVERGENiHBG052487.
InParanoidiP60762.
KOiK11339.
OMAiTGTDANT.
OrthoDBiEOG091G0J32.
PhylomeDBiP60762.
TreeFamiTF323400.

Miscellaneous databases

PROiP60762.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000062270.
CleanExiMM_MORF4L1.
ExpressionAtlasiP60762. baseline and differential.
GenevisibleiP60762. MM.

Family and domain databases

InterProiIPR016197. Chromodomain-like.
IPR008676. MRG.
IPR026541. MRG_dom.
IPR025995. Tudor-knot.
[Graphical view]
PANTHERiPTHR10880. PTHR10880. 2 hits.
PfamiPF05712. MRG. 1 hit.
PF11717. Tudor-knot. 1 hit.
[Graphical view]
PIRSFiPIRSF038133. HAT_Nua4_EAF3/MRG15. 1 hit.
SUPFAMiSSF54160. SSF54160. 2 hits.
PROSITEiPS51640. MRG. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMO4L1_MOUSE
AccessioniPrimary (citable) accession number: P60762
Secondary accession number(s): Q8BNY9, Q99MQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: February 15, 2005
Last modified: November 2, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.