Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Neurogranin

Gene

Nrgn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates the affinity of calmodulin for calcium. Involved in synaptic plasticity and spatial learning.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei38 – 381Crucial for interaction with calmodulin

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calmodulin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Neurogranin
Short name:
Ng
Alternative name(s):
RC3
Cleaved into the following chain:
Gene namesi
Name:Nrgn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1927184. Nrgn.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi38 – 381R → A: Abolishes calmodulin binding, unable to potentiate synaptic transmission. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7878NeurograninPRO_0000159592Add
BLAST
Peptidei55 – 7824NEUG(55-78)By similarityPRO_0000377702Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Disulfide bondi3 ↔ 51Or C-51 with C-4 or C-9
Modified residuei36 – 361Phosphoserine; by PHK and PKC1 Publication
Modified residuei68 – 681Citrulline; partialBy similarity

Post-translational modificationi

Disulfide bond formation is redox-sensitive. The cysteine residues are readily oxidized by several nitric acid (NO) donors and other oxidants to form intramolecular disulfide. Cys-51 can form a disulfide with any other of the cysteine residues with an order of reactivity Cys-9 > Cys-4 > Cys-3 (By similarity).By similarity
Phosphorylated at Ser-36 by PHK and PKC, phosphorylation prevents interaction with Calmodulin and interrupts several learning- and memory-associated functions.1 Publication

Keywords - PTMi

Acetylation, Citrullination, Disulfide bond, Phosphoprotein

Proteomic databases

MaxQBiP60761.
PaxDbiP60761.
PeptideAtlasiP60761.
PRIDEiP60761.

PTM databases

iPTMnetiP60761.
PhosphoSiteiP60761.

Expressioni

Gene expression databases

BgeeiENSMUSG00000053310.
CleanExiMM_NRGN.
GenevisibleiP60761. MM.

Interactioni

Subunit structurei

Interacts with apo-calmodulin; this interaction decreases the affinity of calmodulin for calcium ions.1 Publication

GO - Molecular functioni

  • calmodulin binding Source: MGI

Protein-protein interaction databases

BioGridi211017. 1 interaction.
IntActiP60761. 2 interactions.
STRINGi10090.ENSMUSP00000070113.

Structurei

Secondary structure

1
78
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi28 – 4518Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4E50X-ray2.70A25-50[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 4722IQPROSITE-ProRule annotationAdd
BLAST
Domaini48 – 7831Collagen-likeAdd
BLAST

Domaini

Neurogranin is intrinsically unstructured; however, upon binding with CaM, The IQ domain adopts a helical conformation.

Sequence similaritiesi

Belongs to the neurogranin family.Curated
Contains 1 collagen-like domain.Curated
Contains 1 IQ domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410JNUP. Eukaryota.
ENOG41118RJ. LUCA.
GeneTreeiENSGT00440000039324.
HOGENOMiHOG000113769.
HOVERGENiHBG000470.
InParanoidiP60761.
OMAiPQESACS.
PhylomeDBiP60761.
TreeFamiTF342962.

Family and domain databases

InterProiIPR000048. IQ_motif_EF-hand-BS.
[Graphical view]
PfamiPF00612. IQ. 1 hit.
[Graphical view]
SMARTiSM00015. IQ. 1 hit.
[Graphical view]
PROSITEiPS50096. IQ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60761-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDCCTESACS KPDDDILDIP LDDPGANAAA AKIQASFRGH MARKKIKSGE
60 70
CGRKGPGPGG PGGAGGARGG AGGGPSGD
Length:78
Mass (Da):7,496
Last modified:April 13, 2004 - v1
Checksum:i8E47CDB38E095794
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF230869 Genomic DNA. Translation: AAG27519.1.
AK002933 mRNA. Translation: BAB22466.1.
AK158630 mRNA. Translation: BAE34589.1.
BC061102 mRNA. Translation: AAH61102.1.
BC138511 mRNA. Translation: AAI38512.1.
BC138513 mRNA. Translation: AAI38514.1.
CCDSiCCDS40585.1.
RefSeqiNP_071312.1. NM_022029.2.
UniGeneiMm.335065.

Genome annotation databases

EnsembliENSMUST00000065668; ENSMUSP00000070113; ENSMUSG00000053310.
GeneIDi64011.
KEGGimmu:64011.
UCSCiuc009ovc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF230869 Genomic DNA. Translation: AAG27519.1.
AK002933 mRNA. Translation: BAB22466.1.
AK158630 mRNA. Translation: BAE34589.1.
BC061102 mRNA. Translation: AAH61102.1.
BC138511 mRNA. Translation: AAI38512.1.
BC138513 mRNA. Translation: AAI38514.1.
CCDSiCCDS40585.1.
RefSeqiNP_071312.1. NM_022029.2.
UniGeneiMm.335065.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4E50X-ray2.70A25-50[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211017. 1 interaction.
IntActiP60761. 2 interactions.
STRINGi10090.ENSMUSP00000070113.

PTM databases

iPTMnetiP60761.
PhosphoSiteiP60761.

Proteomic databases

MaxQBiP60761.
PaxDbiP60761.
PeptideAtlasiP60761.
PRIDEiP60761.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000065668; ENSMUSP00000070113; ENSMUSG00000053310.
GeneIDi64011.
KEGGimmu:64011.
UCSCiuc009ovc.1. mouse.

Organism-specific databases

CTDi4900.
MGIiMGI:1927184. Nrgn.

Phylogenomic databases

eggNOGiENOG410JNUP. Eukaryota.
ENOG41118RJ. LUCA.
GeneTreeiENSGT00440000039324.
HOGENOMiHOG000113769.
HOVERGENiHBG000470.
InParanoidiP60761.
OMAiPQESACS.
PhylomeDBiP60761.
TreeFamiTF342962.

Miscellaneous databases

ChiTaRSiNrgn. mouse.
PROiP60761.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000053310.
CleanExiMM_NRGN.
GenevisibleiP60761. MM.

Family and domain databases

InterProiIPR000048. IQ_motif_EF-hand-BS.
[Graphical view]
PfamiPF00612. IQ. 1 hit.
[Graphical view]
SMARTiSM00015. IQ. 1 hit.
[Graphical view]
PROSITEiPS50096. IQ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNEUG_MOUSE
AccessioniPrimary (citable) accession number: P60761
Secondary accession number(s): B2RRN7, Q3TYH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: September 7, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.