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Protein

Lipid A export ATP-binding/permease protein MsbA

Gene

msbA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.3 Publications

Enzyme regulationi

ATPase activity is stimulated by hexaacylated lipid A. Inhibited by vanadate.1 Publication

Kineticsi

  1. KM=878 µM for ATP1 Publication
  1. Vmax=37 nmol/min/mg enzyme1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi376 – 3838ATPUniRule annotation

GO - Molecular functioni

  • ATP binding Source: EcoCyc
  • drug binding Source: EcoliWiki
  • lipid binding Source: EcoliWiki
  • lipid transporter activity Source: EcoCyc
  • lipid-transporting ATPase activity Source: EcoliWiki
  • lipopolysaccharide-transporting ATPase activity Source: EcoCyc

GO - Biological processi

  • lipid transport Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10613-MONOMER.
ECOL316407:JW0897-MONOMER.
MetaCyc:EG10613-MONOMER.
BRENDAi3.6.3.1. 2026.

Protein family/group databases

TCDBi3.A.1.106.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid A export ATP-binding/permease protein MsbAUniRule annotationCurated (EC:3.6.3.-UniRule annotationCurated)
Gene namesi
Name:msbA1 PublicationUniRule annotation
Ordered Locus Names:b0914, JW0897
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10613. msbA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei63 – 8321HelicalUniRule annotationAdd
BLAST
Transmembranei140 – 16223HelicalUniRule annotationAdd
BLAST
Transmembranei167 – 18620HelicalUniRule annotationAdd
BLAST
Transmembranei253 – 27321HelicalUniRule annotationAdd
BLAST
Transmembranei275 – 29521HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  • ATP-binding cassette (ABC) transporter complex Source: UniProtKB-HAMAP
  • membrane Source: UniProtKB
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi270 – 2701A → T: Loss of lipid export. 1 Publication
Mutagenesisi511 – 5111L → P: Loss of ATPase activity; ATP is still bound. 1 Publication
Mutagenesisi512 – 5121D → G: Loss of ATPase activity; ATP is still bound. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 582582Lipid A export ATP-binding/permease protein MsbAPRO_0000092577Add
BLAST

Proteomic databases

PaxDbiP60752.
PRIDEiP60752.

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4260013. 191 interactions.
DIPiDIP-36229N.
IntActiP60752. 2 interactions.
MINTiMINT-1277336.
STRINGi511145.b0914.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3B5WX-ray5.30A/B/C/D/E/F/G/H1-582[»]
DisProtiDP00400.
ProteinModelPortaliP60752.
SMRiP60752. Positions 10-581.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60752.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 310284ABC transmembrane type-1UniRule annotationAdd
BLAST
Domaini342 – 578237ABC transporterUniRule annotationAdd
BLAST

Domaini

In MsbA the ATP-binding domain (NBD) and the transmembrane domain (TMD) are fused.Curated

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Lipid exporter (TC 3.A.1.106) family. [View classification]UniRule annotation
Contains 1 ABC transmembrane type-1 domain.UniRule annotation
Contains 1 ABC transporter domain.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZ1. Bacteria.
COG1132. LUCA.
InParanoidiP60752.
KOiK11085.
OMAiENCAVVS.
PhylomeDBiP60752.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR011917. ABC_transpr_lipidA.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR02203. MsbA_lipidA. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51239. MSBA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P60752-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHNDKDLSTW QTFRRLWPTI APFKAGLIVA GVALILNAAS DTFMLSLLKP
60 70 80 90 100
LLDDGFGKTD RSVLVWMPLV VIGLMILRGI TSYVSSYCIS WVSGKVVMTM
110 120 130 140 150
RRRLFGHMMG MPVSFFDKQS TGTLLSRITY DSEQVASSSS GALITVVREG
160 170 180 190 200
ASIIGLFIMM FYYSWQLSII LIVLAPIVSI AIRVVSKRFR NISKNMQNTM
210 220 230 240 250
GQVTTSAEQM LKGHKEVLIF GGQEVETKRF DKVSNRMRLQ GMKMVSASSI
260 270 280 290 300
SDPIIQLIAS LALAFVLYAA SFPSVMDSLT AGTITVVFSS MIALMRPLKS
310 320 330 340 350
LTNVNAQFQR GMAACQTLFT ILDSEQEKDE GKRVIERATG DVEFRNVTFT
360 370 380 390 400
YPGRDVPALR NINLKIPAGK TVALVGRSGS GKSTIASLIT RFYDIDEGEI
410 420 430 440 450
LMDGHDLREY TLASLRNQVA LVSQNVHLFN DTVANNIAYA RTEQYSREQI
460 470 480 490 500
EEAARMAYAM DFINKMDNGL DTVIGENGVL LSGGQRQRIA IARALLRDSP
510 520 530 540 550
ILILDEATSA LDTESERAIQ AALDELQKNR TSLVIAHRLS TIEKADEIVV
560 570 580
VEDGVIVERG THNDLLEHRG VYAQLHKMQF GQ
Length:582
Mass (Da):64,461
Last modified:April 13, 2004 - v1
Checksum:i3D52A3482174A7FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11796 Genomic DNA. Translation: CAA77839.1.
U00096 Genomic DNA. Translation: AAC74000.1.
AP009048 Genomic DNA. Translation: BAA35658.1.
PIRiC85617.
E90753.
S27998.
RefSeqiNP_415434.1. NC_000913.3.
WP_000551270.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74000; AAC74000; b0914.
BAA35658; BAA35658; BAA35658.
GeneIDi945530.
KEGGiecj:JW0897.
eco:b0914.
PATRICi32117041. VBIEscCol129921_0945.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11796 Genomic DNA. Translation: CAA77839.1.
U00096 Genomic DNA. Translation: AAC74000.1.
AP009048 Genomic DNA. Translation: BAA35658.1.
PIRiC85617.
E90753.
S27998.
RefSeqiNP_415434.1. NC_000913.3.
WP_000551270.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3B5WX-ray5.30A/B/C/D/E/F/G/H1-582[»]
DisProtiDP00400.
ProteinModelPortaliP60752.
SMRiP60752. Positions 10-581.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260013. 191 interactions.
DIPiDIP-36229N.
IntActiP60752. 2 interactions.
MINTiMINT-1277336.
STRINGi511145.b0914.

Protein family/group databases

TCDBi3.A.1.106.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP60752.
PRIDEiP60752.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74000; AAC74000; b0914.
BAA35658; BAA35658; BAA35658.
GeneIDi945530.
KEGGiecj:JW0897.
eco:b0914.
PATRICi32117041. VBIEscCol129921_0945.

Organism-specific databases

EchoBASEiEB0608.
EcoGeneiEG10613. msbA.

Phylogenomic databases

eggNOGiENOG4105BZ1. Bacteria.
COG1132. LUCA.
InParanoidiP60752.
KOiK11085.
OMAiENCAVVS.
PhylomeDBiP60752.

Enzyme and pathway databases

BioCyciEcoCyc:EG10613-MONOMER.
ECOL316407:JW0897-MONOMER.
MetaCyc:EG10613-MONOMER.
BRENDAi3.6.3.1. 2026.

Miscellaneous databases

EvolutionaryTraceiP60752.
PROiP60752.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR011917. ABC_transpr_lipidA.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR02203. MsbA_lipidA. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51239. MSBA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMSBA_ECOLI
AccessioniPrimary (citable) accession number: P60752
Secondary accession number(s): P27299
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: September 7, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.