P60725 (RL4_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
50S ribosomal protein L4

Gene names

Name:	rplD
Ordered Locus Names:	Z4690, ECs4184

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome By similarity. HAMAP MF_01328_B Protein L4 is a both a transcriptional repressor and a translational repressor protein. It regulates transcription of the S10 operon (to which L4 belongs) by causing premature termination of transcription within the S10 leader. L4 controls the translation of the S10 operon by binding to its mRNA By similarity. HAMAP MF_01328_B Forms part of the polypeptide exit tunnel By similarity. HAMAP MF_01328_B
Subunit structure	Part of the 50S ribosomal subunit.
Sequence similarities	Belongs to the ribosomal protein L4P family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Transcription termination Translation regulation
Ligand	RNA-binding rRNA-binding
Molecular function	Repressor Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW transcription termination, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW translation Inferred from electronic annotation. Source: InterPro
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 201	201	50S ribosomal protein L4 HAMAP MF_01328_B		PRO_0000129217

Sequences

	Sequence		Length	Mass (Da)
	P60725 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 3A953206B0F083B5	FASTA	201	22,087
	10 20 30 40 50 60 MELVLKDAQS ALTVSETTFG RDFNEALVHQ VVVAYAAGAR QGTRAQKTRA EVTGSGKKPW 70 80 90 100 110 120 RQKGTGRARS GSIKSPIWRS GGVTFAARPQ DHSQKVNKKM YRGALKSILS ELVRQDRLIV 130 140 150 160 170 180 VEKFSVEAPK TKLLAQKLKD MALEDVLIIT GELDENLFLA ARNLHKVDVR DATGIDPVSL 190 200 IAFDKVVMTA DAVKQVEEML A

References

[1]	"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[2]	"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG58440.1. BA000007 Genomic DNA. Translation: BAB37607.1.
PIR	D85997. H91151.
RefSeq	NP_289880.1. NC_002655.2. NP_312211.1. NC_002695.1.
3D structure databases
ProteinModelPortal	P60725.
SMR	P60725. Positions 1-201.
ModBase	Search...
Proteomic databases
PRIDE	P60725.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000026191; EBESCP00000025084; EBESCG00000025244. EBESCT00000056375; EBESCP00000054203; EBESCG00000055423.
GeneID	915964. 961496.
GenomeReviews	Gene locus Z4690 in contig AE005174_GR. Gene locus ECs4184 in contig BA000007_GR.
KEGG	ece:Z4690. ecs:ECs4184.
PATRIC	18357916. VBIEscCol44059_4125.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000011932.
HOGENOM	HBG545479.
OMA	QAHSRQG.
ProtClustDB	PRK05319.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS4184-MONOMER.
Family and domain databases
HAMAP	MF_01328_B. Ribosomal_L4_B. [Tree]
InterPro	IPR002136. Ribosomal_L4/L1e. IPR013005. Ribosomal_L4/L1e_bac-type. IPR023574. Ribosomal_L4_dom. [Graphical view]
Gene3D	G3DSA:3.40.1370.10. G3DSA:3.40.1370.10. 1 hit.
KO	K02926.
PANTHER	PTHR10746. Ribos_L4_L1E_bac. 1 hit.
Pfam	PF00573. Ribosomal_L4. 1 hit. [Graphical view]
SUPFAM	SSF52166. Ribosomal_L4/L1E. 1 hit.
TIGRFAMs	TIGR03953. RplD_bact. 1 hit.
ProtoNet	Search...
Other
DrugBank	DB00207. Azithromycin. DB00199. Erythromycin.

Entry information

Entry name

RL4_ECO57

Accession

Primary (citable) accession number: P60725
Secondary accession number(s): P02388

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program