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Protein

Octanoyltransferase

Gene

lipB

Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.UniRule annotation

Catalytic activityi

Octanoyl-[acyl-carrier-protein] + protein = protein N(6)-(octanoyl)lysine + [acyl-carrier-protein].UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 1 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (lipB)
  2. Lipoyl synthase (lipA)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei135Lowers pKa of active site CysUniRule annotation1
Active sitei169Acyl-thioester intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciECOL199310:C0720-MONOMER.
UniPathwayiUPA00538; UER00592.

Names & Taxonomyi

Protein namesi
Recommended name:
OctanoyltransferaseUniRule annotation (EC:2.3.1.181UniRule annotation)
Alternative name(s):
Lipoate-protein ligase BUniRule annotation
Lipoyl/octanoyl transferaseUniRule annotation
Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferaseUniRule annotation
Gene namesi
Name:lipBUniRule annotation
Ordered Locus Names:c0720
OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Taxonomic identifieri199310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000001410 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000628361 – 213OctanoyltransferaseAdd BLAST213

Interactioni

Protein-protein interaction databases

STRINGi199310.c0720.

Structurei

3D structure databases

ProteinModelPortaliP60722.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 207BPL/LPL catalyticPROSITE-ProRule annotationAdd BLAST176

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni71 – 78Substrate bindingUniRule annotation8
Regioni138 – 140Substrate bindingUniRule annotation3
Regioni151 – 153Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the LipB family.UniRule annotation
Contains 1 BPL/LPL catalytic domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108BDP. Bacteria.
COG0321. LUCA.
HOGENOMiHOG000194321.
KOiK03801.
OMAiDEKKVCS.

Family and domain databases

HAMAPiMF_00013. LipB. 1 hit.
InterProiIPR004143. BPL_LPL_catalytic.
IPR000544. Octanoyltransferase.
IPR020605. Octanoyltransferase_CS.
[Graphical view]
PfamiPF03099. BPL_LplA_LipB. 1 hit.
[Graphical view]
PIRSFiPIRSF016262. LPLase. 1 hit.
TIGRFAMsiTIGR00214. lipB. 1 hit.
PROSITEiPS51733. BPL_LPL_CATALYTIC. 1 hit.
PS01313. LIPB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P60722-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYQDKILVRQ LGLQPYEPIS QAMHEFTDTR DDSTLDEIWL VEHYPVFTQG
60 70 80 90 100
QAGKAEHILM PGDIPVIQSD RGGQVTYHGP GQQVMYVLLN LKRRKLGVRE
110 120 130 140 150
LVTLLEQTVV NTLAELGIEA HPRADAPGVY VGEKKICSLG LRIRRGCSFH
160 170 180 190 200
GLALNVNMDL SPFLRINPCG YAGMEMAKIS QWKPEATTNN IAPRLLENIL
210
ALLNNPDFEY ITA
Length:213
Mass (Da):23,883
Last modified:April 26, 2004 - v1
Checksum:i56EE2BB105F99EAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA. Translation: AAN79193.1.
RefSeqiWP_000284027.1. NC_004431.1.

Genome annotation databases

EnsemblBacteriaiAAN79193; AAN79193; c0720.
KEGGiecc:c0720.
PATRICi18279479. VBIEscCol75197_0683.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA. Translation: AAN79193.1.
RefSeqiWP_000284027.1. NC_004431.1.

3D structure databases

ProteinModelPortaliP60722.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi199310.c0720.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN79193; AAN79193; c0720.
KEGGiecc:c0720.
PATRICi18279479. VBIEscCol75197_0683.

Phylogenomic databases

eggNOGiENOG4108BDP. Bacteria.
COG0321. LUCA.
HOGENOMiHOG000194321.
KOiK03801.
OMAiDEKKVCS.

Enzyme and pathway databases

UniPathwayiUPA00538; UER00592.
BioCyciECOL199310:C0720-MONOMER.

Family and domain databases

HAMAPiMF_00013. LipB. 1 hit.
InterProiIPR004143. BPL_LPL_catalytic.
IPR000544. Octanoyltransferase.
IPR020605. Octanoyltransferase_CS.
[Graphical view]
PfamiPF03099. BPL_LplA_LipB. 1 hit.
[Graphical view]
PIRSFiPIRSF016262. LPLase. 1 hit.
TIGRFAMsiTIGR00214. lipB. 1 hit.
PROSITEiPS51733. BPL_LPL_CATALYTIC. 1 hit.
PS01313. LIPB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLIPB_ECOL6
AccessioniPrimary (citable) accession number: P60722
Secondary accession number(s): P30976, P77684, Q8XBQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

In the reaction, the free carboxyl group of octanoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes.UniRule annotation

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.