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Protein

Actin, cytoplasmic 1

Gene

ACTB

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-GGA-190873. Gap junction degradation.
R-GGA-196025. Formation of annular gap junctions.
R-GGA-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-GGA-3928662. EPHB-mediated forward signaling.
R-GGA-3928665. EPH-ephrin mediated repulsion of cells.
R-GGA-4420097. VEGFA-VEGFR2 Pathway.
R-GGA-5626467. RHO GTPases activate IQGAPs.
R-GGA-5663213. RHO GTPases Activate WASPs and WAVEs.
R-GGA-5696394. DNA Damage Recognition in GG-NER.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin, cytoplasmic 1
Alternative name(s):
Beta-actin
Cleaved into the following chain:
Gene namesi
Name:ACTB
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 14

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: AgBase
  • cytoskeleton Source: AgBase
  • dense body Source: AgBase
  • focal adhesion Source: AgBase
  • lamellipodium Source: AgBase
  • plasma membrane Source: AgBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 375375Actin, cytoplasmic 1PRO_0000367086Add
BLAST
Initiator methionineiRemoved; alternate1 Publication
Chaini2 – 375374Actin, cytoplasmic 1, N-terminally processedPRO_0000000789Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine; in Actin, cytoplasmic 1; alternateBy similarity
Modified residuei2 – 21N-acetylaspartate; in Actin, cytoplasmic 1, N-terminally processed1 Publication
Modified residuei44 – 441Methionine (R)-sulfoxideBy similarity
Modified residuei47 – 471Methionine (R)-sulfoxideBy similarity
Modified residuei73 – 731Tele-methylhistidineBy similarity

Post-translational modificationi

Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).By similarity

Keywords - PTMi

Acetylation, Methylation, Oxidation

Proteomic databases

PaxDbiP60706.
PRIDEiP60706.

Expressioni

Gene expression databases

ExpressionAtlasiP60706. baseline and differential.

Interactioni

Subunit structurei

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.

Protein-protein interaction databases

BioGridi676771. 2 interactions.
DIPiDIP-41284N.
MINTiMINT-199239.
STRINGi9031.ENSGALP00000039176.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J0Selectron microscopy9.00A/B/C/D/E/F/G/H/I/J/K/L2-375[»]
ProteinModelPortaliP60706.
SMRiP60706. Positions 6-375.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family.Curated

Phylogenomic databases

eggNOGiKOG0676. Eukaryota.
COG5277. LUCA.
GeneTreeiENSGT00760000118957.
HOGENOMiHOG000233340.
HOVERGENiHBG003771.
InParanoidiP60706.
KOiK05692.
OMAiMCKAGCA.

Family and domain databases

InterProiIPR004000. Actin.
IPR020902. Actin/actin-like_CS.
IPR004001. Actin_CS.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
PRINTSiPR00190. ACTIN.
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEiPS00406. ACTINS_1. 1 hit.
PS00432. ACTINS_2. 1 hit.
PS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60706-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDDIAALVV DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGVMVGMGQK
60 70 80 90 100
DSYVGDEAQS KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE
110 120 130 140 150
HPVLLTEAPL NPKANREKMT QIMFETFNTP AMYVAIQAVL SLYASGRTTG
160 170 180 190 200
IVMDSGDGVT HTVPIYEGYA LPHAILRLDL AGRDLTDYLM KILTERGYSF
210 220 230 240 250
TTTAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY ELPDGQVITI
260 270 280 290 300
GNERFRCPEA LFQPSFLGME SCGIHETTFN SIMKCDVDIR KDLYANTVLS
310 320 330 340 350
GGTTMYPGIA DRMQKEITAL APSTMKIKII APPERKYSVW IGGSILASLS
360 370
TFQQMWISKQ EYDESGPSIV HRKCF
Length:375
Mass (Da):41,737
Last modified:April 1, 1988 - v1
Checksum:i6AFD05CA94E360E2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti336 – 3361K → R in CAA25004 (PubMed:6324080).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00182 Genomic DNA. Translation: CAA25004.1.
L08165 mRNA. Translation: AAA48615.1.
PIRiA20888. ATCHB.
RefSeqiNP_990849.1. NM_205518.1.
UniGeneiGga.48207.
Gga.6076.

Genome annotation databases

EnsembliENSGALT00000015673; ENSGALP00000015657; ENSGALG00000009621.
GeneIDi396526.
KEGGigga:396526.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00182 Genomic DNA. Translation: CAA25004.1.
L08165 mRNA. Translation: AAA48615.1.
PIRiA20888. ATCHB.
RefSeqiNP_990849.1. NM_205518.1.
UniGeneiGga.48207.
Gga.6076.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J0Selectron microscopy9.00A/B/C/D/E/F/G/H/I/J/K/L2-375[»]
ProteinModelPortaliP60706.
SMRiP60706. Positions 6-375.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi676771. 2 interactions.
DIPiDIP-41284N.
MINTiMINT-199239.
STRINGi9031.ENSGALP00000039176.

Proteomic databases

PaxDbiP60706.
PRIDEiP60706.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000015673; ENSGALP00000015657; ENSGALG00000009621.
GeneIDi396526.
KEGGigga:396526.

Organism-specific databases

CTDi60.

Phylogenomic databases

eggNOGiKOG0676. Eukaryota.
COG5277. LUCA.
GeneTreeiENSGT00760000118957.
HOGENOMiHOG000233340.
HOVERGENiHBG003771.
InParanoidiP60706.
KOiK05692.
OMAiMCKAGCA.

Enzyme and pathway databases

ReactomeiR-GGA-190873. Gap junction degradation.
R-GGA-196025. Formation of annular gap junctions.
R-GGA-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-GGA-3928662. EPHB-mediated forward signaling.
R-GGA-3928665. EPH-ephrin mediated repulsion of cells.
R-GGA-4420097. VEGFA-VEGFR2 Pathway.
R-GGA-5626467. RHO GTPases activate IQGAPs.
R-GGA-5663213. RHO GTPases Activate WASPs and WAVEs.
R-GGA-5696394. DNA Damage Recognition in GG-NER.

Miscellaneous databases

NextBioi20805081.
PROiP60706.

Gene expression databases

ExpressionAtlasiP60706. baseline and differential.

Family and domain databases

InterProiIPR004000. Actin.
IPR020902. Actin/actin-like_CS.
IPR004001. Actin_CS.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
PRINTSiPR00190. ACTIN.
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEiPS00406. ACTINS_1. 1 hit.
PS00432. ACTINS_2. 1 hit.
PS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The nucleotide sequence of the chick cytoplasmic beta-actin gene."
    Kost T.A., Theodorakis N., Hughes S.H.
    Nucleic Acids Res. 11:8287-8301(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequence of a beta-actin cDNA from mitochondrial DNA-depleted chicken cells."
    Wang H., Morais R.
    Submitted (JAN-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Bienvenut W.V., Black E.J., Gillespie D.A.
    Submitted (JAN-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-37; 85-113; 178-191; 197-206; 239-254; 291-326 AND 360-372, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ASP-2, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: B-cell lymphoma.

Entry informationi

Entry nameiACTB_CHICK
AccessioniPrimary (citable) accession number: P60706
Secondary accession number(s): P02570
, P70514, P99021, Q11211, Q64316
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 1, 1988
Last modified: May 11, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.