Reviewed, UniProtKB/Swiss-Prot P60706 (ACTB_CHICK)

Names and origin

Protein names

Recommended name:
    Actin, cytoplasmic 1
Alternative name(s):
    Beta-actin
Cleaved into the following chain:
    1- Recommended name:
            Actin, cytoplasmic 1, N-terminally processed

Gene names

Name:

ACTB

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subunit structure	Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
Subcellular location	Cytoplasm › cytoskeleton.
Miscellaneous	In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Sequence similarities	Belongs to the actin family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Ligand	ATP-binding Nucleotide-binding
PTM	Acetylation Methylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Actin, cytoplasmic 1		PRO_0000367086
	Initiator methionine	1	1	Removed; alternate Ref.3
	Chain	2 – 375	374	Actin, cytoplasmic 1, N-terminally processed		PRO_0000000789
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine; in Actin, cytoplasmic 1; alternate By similarity
	Modified residue	2	1	N-acetylaspartate; in Actin, cytoplasmic 1, N-terminally processed Ref.3
	Modified residue	73	1	Tele-methylhistidine By similarity
Experimental info
	Sequence conflict	336	1	K → R in CAA25004. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P60706-1 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: 6AFD05CA94E360E2	FASTA	375	41,737
	10 20 30 40 50 60 MDDDIAALVV DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGVMVGMGQK DSYVGDEAQS 70 80 90 100 110 120 KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE HPVLLTEAPL NPKANREKMT 130 140 150 160 170 180 QIMFETFNTP AMYVAIQAVL SLYASGRTTG IVMDSGDGVT HTVPIYEGYA LPHAILRLDL 190 200 210 220 230 240 AGRDLTDYLM KILTERGYSF TTTAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY 250 260 270 280 290 300 ELPDGQVITI GNERFRCPEA LFQPSFLGME SCGIHETTFN SIMKCDVDIR KDLYANTVLS 310 320 330 340 350 360 GGTTMYPGIA DRMQKEITAL APSTMKIKII APPERKYSVW IGGSILASLS TFQQMWISKQ 370 EYDESGPSIV HRKCF

References

[1]	"The nucleotide sequence of the chick cytoplasmic beta-actin gene." Kost T.A., Theodorakis N., Hughes S.H. Nucleic Acids Res. 11:8287-8301(1983) [PubMed: 6324080] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence of a beta-actin cDNA from mitochondrial DNA-depleted chicken cells." Wang H., Morais R. Submitted (JAN-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	Bienvenut W.V., Black E.J., Gillespie D.A. Submitted (JAN-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-37; 85-113; 178-191; 197-206; 239-254; 291-326 AND 360-372, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ASP-2, MASS SPECTROMETRY. Tissue: B-cell lymphoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X00182 Genomic DNA. Translation: CAA25004.1. L08165 mRNA. Translation: AAA48615.1.
IPI	IPI00655503.
PIR	ATCHB. A20888.
RefSeq	NP_990849.1.
UniGene	Gga.6076
3D structure databases
SMR	P60706. Positions 4-371.
ModBase	Search...
Protein-protein interaction databases
STRING	P60706.
Proteomic databases
PRIDE	P60706.
Genome annotation databases
Ensembl	ENSGALT00000002103; ENSGALP00000002101; ENSGALG00000001381; Gallus gallus. [Genome view] ENSGALT00000039969; ENSGALP00000039176; ENSGALG00000001381; Gallus gallus. [Genome view]
GeneID	396526.
KEGG	gga:396526.
Organism-specific databases
CTD	396526.
Phylogenomic databases
HOVERGEN	P60706.
Family and domain databases
InterPro	IPR004000. Actin-like. IPR004001. Actin_CS. [Graphical view]
PANTHER	PTHR11937. Actin_like. 1 hit.
Pfam	PF00022. Actin. 1 hit. [Graphical view]
PRINTS	PR00190. ACTIN.
SMART	SM00268. ACTIN. 1 hit. [Graphical view]
PROSITE	PS00406. ACTINS_1. 1 hit. PS00432. ACTINS_2. 1 hit. PS01132. ACTINS_ACT_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACTB_CHICK

Accession

Primary (citable) accession number: P60706
Secondary accession number(s): P02570 , P70514, P99021, Q11211, Q64316

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	April 1, 1988
Last modified:	November 24, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)