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P60670

- NPL4_MOUSE

UniProt

P60670 - NPL4_MOUSE

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Protein

Nuclear protein localization protein 4 homolog

Gene
Nploc4, Kiaa1499, Npl4
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope By similarity.

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri580 – 60829RanBP2-typeAdd
BLAST

GO - Molecular functioni

  1. zinc ion binding Source: InterPro

GO - Biological processi

  1. ER-associated ubiquitin-dependent protein catabolic process Source: HGNC
  2. Golgi organization Source: HGNC
  3. membrane fusion Source: HGNC
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00144.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear protein localization protein 4 homolog
Short name:
Protein NPL4
Gene namesi
Name:Nploc4
Synonyms:Kiaa1499, Npl4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:2679787. Nploc4.

Subcellular locationi

Cytoplasmcytosol By similarity. Endoplasmic reticulum By similarity. Nucleus By similarity
Note: Associated with the endoplasmic reticulum and nuclear By similarity.

GO - Cellular componenti

  1. cytosol Source: UniProtKB-SubCell
  2. endoplasmic reticulum Source: HGNC
  3. nuclear outer membrane-endoplasmic reticulum membrane network Source: HGNC
  4. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 608607Nuclear protein localization protein 4 homologPRO_0000057942Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei179 – 1791N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP60670.
PaxDbiP60670.
PRIDEiP60670.

Expressioni

Gene expression databases

BgeeiP60670.
GenevestigatoriP60670.

Interactioni

Subunit structurei

Heterodimer with UFD1L. The heterodimer binds ubiquitinated proteins. The heterodimer binds to VCP and inhibits Golgi membrane fusion By similarity.

Protein-protein interaction databases

BioGridi229901. 4 interactions.
STRINGi10090.ENSMUSP00000035851.

Structurei

Secondary structure

1
608
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 103
Beta strandi12 – 176
Helixi26 – 3611
Turni41 – 433
Helixi52 – 554
Beta strandi56 – 583
Turni63 – 675

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PJHNMR-A1-80[»]
ProteinModelPortaliP60670.
SMRiP60670. Positions 2-77, 578-608.

Miscellaneous databases

EvolutionaryTraceiP60670.

Family & Domainsi

Domaini

Binds ubiquitinated proteins via its RanBP2-type zinc finger.

Sequence similaritiesi

Belongs to the NPL4 family.

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5100.
GeneTreeiENSGT00390000018731.
HOGENOMiHOG000008139.
HOVERGENiHBG052659.
InParanoidiB1ATY4.
KOiK14015.
OMAiECITAGM.
OrthoDBiEOG71RXJG.
PhylomeDBiP60670.
TreeFamiTF314173.

Family and domain databases

InterProiIPR007717. NPL4.
IPR024682. Npl4_Ub-like_dom.
IPR007716. NPL4_Zn-bd_put.
IPR016563. PolyUb_recognition_cplx_Npl4.
IPR029071. Ubiquitin-rel_dom.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF05021. NPL4. 1 hit.
PF11543. UN_NPL4. 1 hit.
PF05020. zf-NPL4. 1 hit.
[Graphical view]
PIRSFiPIRSF010052. Polyub_prc_Npl4. 1 hit.
SMARTiSM00547. ZnF_RBZ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P60670-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAESIIIRVQ SPDGVKRITA TKRETAATFL KKVAKEFGFQ NNGFSVYINR    50
NKTGEITASS SKSLHLLKIK HGDLLFLFPS SLAGPSSEME TSTSVGLKAF 100
GAPNVVEDEI DQYLSKQDGK IYRSRDPQLC RHGPLGKCVH CVPLEPFDED 150
YLNHLEPPVK HMSFHAYIRK LTGGADKGKF VALENISCKI KSGCEGHLPW 200
PNGICTKCQP SAITLNRQKY RHVDNIMFEN HTVADRFLDF WRKTGNQHFG 250
YLYGRYTEHK DIPLGIRAEV AAIYEPPQIG TQNSLELLED PKAEVVDEIA 300
AKLGLRKVGW IFTDLVSEDT RKGTVRYSRN KDTYFLSSEE CITAGDFQNK 350
HPNICRLSPD GHFGSKFVTA VATGGPDNQV HFEGYQVSNQ CMALVRDECL 400
LPCKDAPELG YAKESSSEQY VPDVFYKDID KFGNEITQLA RPLPVEYLII 450
DITTTFPKDP VYTFSISQNP FPIENRDVLG ETQDFHSLAT YLSQNTSSVF 500
LDTISDFHLL LFLVTNEVMP LQDSISLLLE AVRTRNEELA QTWKKSEQWA 550
TIEQLCSTVG VQLPGLHEFG AVGGSARAAT SAMWACQHCT FMNQPGTGHC 600
EMCSLPRT 608
Length:608
Mass (Da):68,017
Last modified:January 23, 2007 - v3
Checksum:i378E31B35D559336
GO
Isoform 2 (identifier: P60670-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     452-483: Missing.

Note: No experimental confirmation available.

Show »
Length:576
Mass (Da):64,379
Checksum:i478732FF9B936035
GO

Sequence cautioni

The sequence BAC98185.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei452 – 48332Missing in isoform 2. VSP_009790Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK129375 mRNA. Translation: BAC98185.1. Different initiation.
AL669855 Genomic DNA. Translation: CAM27040.1.
AL669855 Genomic DNA. Translation: CAM27041.1.
BC065156 mRNA. Translation: AAH65156.1.
CCDSiCCDS25732.1. [P60670-2]
CCDS56827.1. [P60670-1]
RefSeqiNP_001181952.1. NM_001195023.1. [P60670-1]
NP_955763.1. NM_199469.2. [P60670-2]
UniGeneiMm.309520.

Genome annotation databases

EnsembliENSMUST00000044271; ENSMUSP00000035851; ENSMUSG00000039703. [P60670-1]
ENSMUST00000103017; ENSMUSP00000099306; ENSMUSG00000039703. [P60670-2]
GeneIDi217365.
KEGGimmu:217365.
UCSCiuc007msp.2. mouse. [P60670-2]
uc007msq.2. mouse. [P60670-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK129375 mRNA. Translation: BAC98185.1 . Different initiation.
AL669855 Genomic DNA. Translation: CAM27040.1 .
AL669855 Genomic DNA. Translation: CAM27041.1 .
BC065156 mRNA. Translation: AAH65156.1 .
CCDSi CCDS25732.1. [P60670-2 ]
CCDS56827.1. [P60670-1 ]
RefSeqi NP_001181952.1. NM_001195023.1. [P60670-1 ]
NP_955763.1. NM_199469.2. [P60670-2 ]
UniGenei Mm.309520.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2PJH NMR - A 1-80 [» ]
ProteinModelPortali P60670.
SMRi P60670. Positions 2-77, 578-608.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 229901. 4 interactions.
STRINGi 10090.ENSMUSP00000035851.

Proteomic databases

MaxQBi P60670.
PaxDbi P60670.
PRIDEi P60670.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000044271 ; ENSMUSP00000035851 ; ENSMUSG00000039703 . [P60670-1 ]
ENSMUST00000103017 ; ENSMUSP00000099306 ; ENSMUSG00000039703 . [P60670-2 ]
GeneIDi 217365.
KEGGi mmu:217365.
UCSCi uc007msp.2. mouse. [P60670-2 ]
uc007msq.2. mouse. [P60670-1 ]

Organism-specific databases

CTDi 55666.
MGIi MGI:2679787. Nploc4.
Rougei Search...

Phylogenomic databases

eggNOGi COG5100.
GeneTreei ENSGT00390000018731.
HOGENOMi HOG000008139.
HOVERGENi HBG052659.
InParanoidi B1ATY4.
KOi K14015.
OMAi ECITAGM.
OrthoDBi EOG71RXJG.
PhylomeDBi P60670.
TreeFami TF314173.

Enzyme and pathway databases

UniPathwayi UPA00144 .

Miscellaneous databases

ChiTaRSi NPLOC4. mouse.
EvolutionaryTracei P60670.
NextBioi 375815.
PROi P60670.
SOURCEi Search...

Gene expression databases

Bgeei P60670.
Genevestigatori P60670.

Family and domain databases

InterProi IPR007717. NPL4.
IPR024682. Npl4_Ub-like_dom.
IPR007716. NPL4_Zn-bd_put.
IPR016563. PolyUb_recognition_cplx_Npl4.
IPR029071. Ubiquitin-rel_dom.
IPR001876. Znf_RanBP2.
[Graphical view ]
Pfami PF05021. NPL4. 1 hit.
PF11543. UN_NPL4. 1 hit.
PF05020. zf-NPL4. 1 hit.
[Graphical view ]
PIRSFi PIRSF010052. Polyub_prc_Npl4. 1 hit.
SMARTi SM00547. ZnF_RBZ. 1 hit.
[Graphical view ]
SUPFAMi SSF54236. SSF54236. 1 hit.
PROSITEi PS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Embryonic tail.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6.
    Tissue: Brain.
  4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-179, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiNPL4_MOUSE
AccessioniPrimary (citable) accession number: P60670
Secondary accession number(s): B1ATY4, B1ATY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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