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P60670

- NPL4_MOUSE

UniProt

P60670 - NPL4_MOUSE

Protein

Nuclear protein localization protein 4 homolog

Gene

Nploc4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope By similarity.By similarity

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri580 – 60829RanBP2-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. zinc ion binding Source: InterPro

    GO - Biological processi

    1. ER-associated ubiquitin-dependent protein catabolic process Source: HGNC
    2. Golgi organization Source: HGNC
    3. membrane fusion Source: HGNC

    Keywords - Molecular functioni

    Chaperone

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00144.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear protein localization protein 4 homolog
    Short name:
    Protein NPL4
    Gene namesi
    Name:Nploc4
    Synonyms:Kiaa1499, Npl4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:2679787. Nploc4.

    Subcellular locationi

    Cytoplasmcytosol By similarity. Endoplasmic reticulum By similarity. Nucleus By similarity
    Note: Associated with the endoplasmic reticulum and nuclear.By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell
    2. endoplasmic reticulum Source: HGNC
    3. nuclear outer membrane-endoplasmic reticulum membrane network Source: HGNC
    4. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 608607Nuclear protein localization protein 4 homologPRO_0000057942Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei179 – 1791N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP60670.
    PaxDbiP60670.
    PRIDEiP60670.

    Expressioni

    Gene expression databases

    BgeeiP60670.
    GenevestigatoriP60670.

    Interactioni

    Subunit structurei

    Heterodimer with UFD1L. The heterodimer binds ubiquitinated proteins. The heterodimer binds to VCP and inhibits Golgi membrane fusion By similarity.By similarity

    Protein-protein interaction databases

    BioGridi229901. 4 interactions.
    STRINGi10090.ENSMUSP00000035851.

    Structurei

    Secondary structure

    1
    608
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi8 – 103
    Beta strandi12 – 176
    Helixi26 – 3611
    Turni41 – 433
    Helixi52 – 554
    Beta strandi56 – 583
    Turni63 – 675

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2PJHNMR-A1-80[»]
    ProteinModelPortaliP60670.
    SMRiP60670. Positions 2-77, 578-608.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP60670.

    Family & Domainsi

    Domaini

    Binds ubiquitinated proteins via its RanBP2-type zinc finger.

    Sequence similaritiesi

    Belongs to the NPL4 family.Curated
    Contains 1 RanBP2-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri580 – 60829RanBP2-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5100.
    GeneTreeiENSGT00390000018731.
    HOGENOMiHOG000008139.
    HOVERGENiHBG052659.
    InParanoidiB1ATY4.
    KOiK14015.
    OMAiECITAGM.
    OrthoDBiEOG71RXJG.
    PhylomeDBiP60670.
    TreeFamiTF314173.

    Family and domain databases

    InterProiIPR007717. NPL4.
    IPR024682. Npl4_Ub-like_dom.
    IPR007716. NPL4_Zn-bd_put.
    IPR016563. PolyUb_recognition_cplx_Npl4.
    IPR029071. Ubiquitin-rel_dom.
    IPR001876. Znf_RanBP2.
    [Graphical view]
    PfamiPF05021. NPL4. 1 hit.
    PF11543. UN_NPL4. 1 hit.
    PF05020. zf-NPL4. 1 hit.
    [Graphical view]
    PIRSFiPIRSF010052. Polyub_prc_Npl4. 1 hit.
    SMARTiSM00547. ZnF_RBZ. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.
    PROSITEiPS01358. ZF_RANBP2_1. 1 hit.
    PS50199. ZF_RANBP2_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P60670-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAESIIIRVQ SPDGVKRITA TKRETAATFL KKVAKEFGFQ NNGFSVYINR    50
    NKTGEITASS SKSLHLLKIK HGDLLFLFPS SLAGPSSEME TSTSVGLKAF 100
    GAPNVVEDEI DQYLSKQDGK IYRSRDPQLC RHGPLGKCVH CVPLEPFDED 150
    YLNHLEPPVK HMSFHAYIRK LTGGADKGKF VALENISCKI KSGCEGHLPW 200
    PNGICTKCQP SAITLNRQKY RHVDNIMFEN HTVADRFLDF WRKTGNQHFG 250
    YLYGRYTEHK DIPLGIRAEV AAIYEPPQIG TQNSLELLED PKAEVVDEIA 300
    AKLGLRKVGW IFTDLVSEDT RKGTVRYSRN KDTYFLSSEE CITAGDFQNK 350
    HPNICRLSPD GHFGSKFVTA VATGGPDNQV HFEGYQVSNQ CMALVRDECL 400
    LPCKDAPELG YAKESSSEQY VPDVFYKDID KFGNEITQLA RPLPVEYLII 450
    DITTTFPKDP VYTFSISQNP FPIENRDVLG ETQDFHSLAT YLSQNTSSVF 500
    LDTISDFHLL LFLVTNEVMP LQDSISLLLE AVRTRNEELA QTWKKSEQWA 550
    TIEQLCSTVG VQLPGLHEFG AVGGSARAAT SAMWACQHCT FMNQPGTGHC 600
    EMCSLPRT 608
    Length:608
    Mass (Da):68,017
    Last modified:January 23, 2007 - v3
    Checksum:i378E31B35D559336
    GO
    Isoform 2 (identifier: P60670-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         452-483: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:576
    Mass (Da):64,379
    Checksum:i478732FF9B936035
    GO

    Sequence cautioni

    The sequence BAC98185.1 differs from that shown. Reason: Erroneous initiation.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei452 – 48332Missing in isoform 2. 1 PublicationVSP_009790Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK129375 mRNA. Translation: BAC98185.1. Different initiation.
    AL669855 Genomic DNA. Translation: CAM27040.1.
    AL669855 Genomic DNA. Translation: CAM27041.1.
    BC065156 mRNA. Translation: AAH65156.1.
    CCDSiCCDS25732.1. [P60670-2]
    CCDS56827.1. [P60670-1]
    RefSeqiNP_001181952.1. NM_001195023.1. [P60670-1]
    NP_955763.1. NM_199469.2. [P60670-2]
    UniGeneiMm.309520.

    Genome annotation databases

    EnsembliENSMUST00000044271; ENSMUSP00000035851; ENSMUSG00000039703. [P60670-1]
    ENSMUST00000103017; ENSMUSP00000099306; ENSMUSG00000039703. [P60670-2]
    GeneIDi217365.
    KEGGimmu:217365.
    UCSCiuc007msp.2. mouse. [P60670-2]
    uc007msq.2. mouse. [P60670-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK129375 mRNA. Translation: BAC98185.1 . Different initiation.
    AL669855 Genomic DNA. Translation: CAM27040.1 .
    AL669855 Genomic DNA. Translation: CAM27041.1 .
    BC065156 mRNA. Translation: AAH65156.1 .
    CCDSi CCDS25732.1. [P60670-2 ]
    CCDS56827.1. [P60670-1 ]
    RefSeqi NP_001181952.1. NM_001195023.1. [P60670-1 ]
    NP_955763.1. NM_199469.2. [P60670-2 ]
    UniGenei Mm.309520.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2PJH NMR - A 1-80 [» ]
    ProteinModelPortali P60670.
    SMRi P60670. Positions 2-77, 578-608.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 229901. 4 interactions.
    STRINGi 10090.ENSMUSP00000035851.

    Proteomic databases

    MaxQBi P60670.
    PaxDbi P60670.
    PRIDEi P60670.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000044271 ; ENSMUSP00000035851 ; ENSMUSG00000039703 . [P60670-1 ]
    ENSMUST00000103017 ; ENSMUSP00000099306 ; ENSMUSG00000039703 . [P60670-2 ]
    GeneIDi 217365.
    KEGGi mmu:217365.
    UCSCi uc007msp.2. mouse. [P60670-2 ]
    uc007msq.2. mouse. [P60670-1 ]

    Organism-specific databases

    CTDi 55666.
    MGIi MGI:2679787. Nploc4.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG5100.
    GeneTreei ENSGT00390000018731.
    HOGENOMi HOG000008139.
    HOVERGENi HBG052659.
    InParanoidi B1ATY4.
    KOi K14015.
    OMAi ECITAGM.
    OrthoDBi EOG71RXJG.
    PhylomeDBi P60670.
    TreeFami TF314173.

    Enzyme and pathway databases

    UniPathwayi UPA00144 .

    Miscellaneous databases

    ChiTaRSi NPLOC4. mouse.
    EvolutionaryTracei P60670.
    NextBioi 375815.
    PROi P60670.
    SOURCEi Search...

    Gene expression databases

    Bgeei P60670.
    Genevestigatori P60670.

    Family and domain databases

    InterProi IPR007717. NPL4.
    IPR024682. Npl4_Ub-like_dom.
    IPR007716. NPL4_Zn-bd_put.
    IPR016563. PolyUb_recognition_cplx_Npl4.
    IPR029071. Ubiquitin-rel_dom.
    IPR001876. Znf_RanBP2.
    [Graphical view ]
    Pfami PF05021. NPL4. 1 hit.
    PF11543. UN_NPL4. 1 hit.
    PF05020. zf-NPL4. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF010052. Polyub_prc_Npl4. 1 hit.
    SMARTi SM00547. ZnF_RBZ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    PROSITEi PS01358. ZF_RANBP2_1. 1 hit.
    PS50199. ZF_RANBP2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
      DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Embryonic tail.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6.
      Tissue: Brain.
    4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-179, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiNPL4_MOUSE
    AccessioniPrimary (citable) accession number: P60670
    Secondary accession number(s): B1ATY4, B1ATY5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 95 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3