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P60670 (NPL4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear protein localization protein 4 homolog

Short name=Protein NPL4
Gene names
Name:Nploc4
Synonyms:Kiaa1499, Npl4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length608 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope By similarity.

Pathway

Protein degradation; proteasomal ubiquitin-dependent pathway.

Subunit structure

Heterodimer with UFD1L. The heterodimer binds ubiquitinated proteins. The heterodimer binds to VCP and inhibits Golgi membrane fusion By similarity.

Subcellular location

Cytoplasmcytosol By similarity. Endoplasmic reticulum By similarity. Nucleus By similarity. Note: Associated with the endoplasmic reticulum and nuclear By similarity.

Domain

Binds ubiquitinated proteins via its RanBP2-type zinc finger.

Sequence similarities

Belongs to the NPL4 family.

Contains 1 RanBP2-type zinc finger.

Sequence caution

The sequence BAC98185.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P60670-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P60670-2)

The sequence of this isoform differs from the canonical sequence as follows:
     452-483: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 608607Nuclear protein localization protein 4 homolog
PRO_0000057942

Regions

Zinc finger580 – 60829RanBP2-type

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue1791N6-acetyllysine Ref.4

Natural variations

Alternative sequence452 – 48332Missing in isoform 2.
VSP_009790

Secondary structure

.............. 608
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 378E31B35D559336

FASTA60868,017
        10         20         30         40         50         60 
MAESIIIRVQ SPDGVKRITA TKRETAATFL KKVAKEFGFQ NNGFSVYINR NKTGEITASS 

        70         80         90        100        110        120 
SKSLHLLKIK HGDLLFLFPS SLAGPSSEME TSTSVGLKAF GAPNVVEDEI DQYLSKQDGK 

       130        140        150        160        170        180 
IYRSRDPQLC RHGPLGKCVH CVPLEPFDED YLNHLEPPVK HMSFHAYIRK LTGGADKGKF 

       190        200        210        220        230        240 
VALENISCKI KSGCEGHLPW PNGICTKCQP SAITLNRQKY RHVDNIMFEN HTVADRFLDF 

       250        260        270        280        290        300 
WRKTGNQHFG YLYGRYTEHK DIPLGIRAEV AAIYEPPQIG TQNSLELLED PKAEVVDEIA 

       310        320        330        340        350        360 
AKLGLRKVGW IFTDLVSEDT RKGTVRYSRN KDTYFLSSEE CITAGDFQNK HPNICRLSPD 

       370        380        390        400        410        420 
GHFGSKFVTA VATGGPDNQV HFEGYQVSNQ CMALVRDECL LPCKDAPELG YAKESSSEQY 

       430        440        450        460        470        480 
VPDVFYKDID KFGNEITQLA RPLPVEYLII DITTTFPKDP VYTFSISQNP FPIENRDVLG 

       490        500        510        520        530        540 
ETQDFHSLAT YLSQNTSSVF LDTISDFHLL LFLVTNEVMP LQDSISLLLE AVRTRNEELA 

       550        560        570        580        590        600 
QTWKKSEQWA TIEQLCSTVG VQLPGLHEFG AVGGSARAAT SAMWACQHCT FMNQPGTGHC 


EMCSLPRT 

« Hide

Isoform 2 [UniParc].

Checksum: 478732FF9B936035
Show »

FASTA57664,379

References

[1]"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Embryonic tail.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6.
Tissue: Brain.
[4]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-179, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK129375 mRNA. Translation: BAC98185.1. Different initiation.
AL669855 Genomic DNA. Translation: CAM27040.1.
AL669855 Genomic DNA. Translation: CAM27041.1.
BC065156 mRNA. Translation: AAH65156.1.
RefSeqNP_001181952.1. NM_001195023.1.
NP_955763.1. NM_199469.2.
UniGeneMm.309520.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2PJHNMR-A1-80[»]
ProteinModelPortalP60670.
SMRP60670. Positions 2-77, 578-608.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid229901. 4 interactions.
STRING10090.ENSMUSP00000035851.

Proteomic databases

PaxDbP60670.
PRIDEP60670.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000044271; ENSMUSP00000035851; ENSMUSG00000039703. [P60670-1]
ENSMUST00000103017; ENSMUSP00000099306; ENSMUSG00000039703. [P60670-2]
GeneID217365.
KEGGmmu:217365.
UCSCuc007msp.2. mouse. [P60670-2]
uc007msq.2. mouse. [P60670-1]

Organism-specific databases

CTD55666.
MGIMGI:2679787. Nploc4.
RougeSearch...

Phylogenomic databases

eggNOGCOG5100.
GeneTreeENSGT00390000018731.
HOGENOMHOG000008139.
HOVERGENHBG052659.
InParanoidB1ATY4.
KOK14015.
OMAECITAGM.
OrthoDBEOG71RXJG.
PhylomeDBP60670.
TreeFamTF314173.

Enzyme and pathway databases

UniPathwayUPA00144.

Gene expression databases

BgeeP60670.
GenevestigatorP60670.

Family and domain databases

InterProIPR007717. NPL4.
IPR024682. Npl4_Ub-like_dom.
IPR007716. NPL4_Zn-bd_put.
IPR016563. PolyUb_recognition_cplx_Npl4.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamPF05021. NPL4. 1 hit.
PF11543. UN_NPL4. 1 hit.
PF05020. zf-NPL4. 1 hit.
[Graphical view]
PIRSFPIRSF010052. Polyub_prc_Npl4. 1 hit.
SMARTSM00547. ZnF_RBZ. 1 hit.
[Graphical view]
PROSITEPS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNPLOC4. mouse.
EvolutionaryTraceP60670.
NextBio375815.
PROP60670.
SOURCESearch...

Entry information

Entry nameNPL4_MOUSE
AccessionPrimary (citable) accession number: P60670
Secondary accession number(s): B1ATY4, B1ATY5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 91 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot