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Protein

Nuclear protein localization protein 4 homolog

Gene

Nploc4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity).By similarity

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri580 – 60829RanBP2-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. zinc ion binding Source: InterPro

GO - Biological processi

  1. ER-associated ubiquitin-dependent protein catabolic process Source: HGNC
  2. Golgi organization Source: HGNC
  3. membrane fusion Source: HGNC
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00144.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear protein localization protein 4 homolog
Short name:
Protein NPL4
Gene namesi
Name:Nploc4
Synonyms:Kiaa1499, Npl4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2679787. Nploc4.

Subcellular locationi

Cytoplasmcytosol By similarity. Endoplasmic reticulum By similarity. Nucleus By similarity
Note: Associated with the endoplasmic reticulum and nuclear.By similarity

GO - Cellular componenti

  1. cytosol Source: UniProtKB-SubCell
  2. endoplasmic reticulum Source: HGNC
  3. nuclear outer membrane-endoplasmic reticulum membrane network Source: HGNC
  4. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 608607Nuclear protein localization protein 4 homologPRO_0000057942Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei179 – 1791N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP60670.
PaxDbiP60670.
PRIDEiP60670.

Expressioni

Gene expression databases

BgeeiP60670.
GenevestigatoriP60670.

Interactioni

Subunit structurei

Heterodimer with UFD1L. The heterodimer binds ubiquitinated proteins. The heterodimer binds to VCP and inhibits Golgi membrane fusion (By similarity).By similarity

Protein-protein interaction databases

BioGridi229901. 5 interactions.
STRINGi10090.ENSMUSP00000035851.

Structurei

Secondary structure

1
608
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 103Combined sources
Beta strandi12 – 176Combined sources
Helixi26 – 3611Combined sources
Turni41 – 433Combined sources
Helixi52 – 554Combined sources
Beta strandi56 – 583Combined sources
Turni63 – 675Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PJHNMR-A1-80[»]
ProteinModelPortaliP60670.
SMRiP60670. Positions 2-77, 578-608.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60670.

Family & Domainsi

Domaini

Binds ubiquitinated proteins via its RanBP2-type zinc finger.

Sequence similaritiesi

Belongs to the NPL4 family.Curated
Contains 1 RanBP2-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri580 – 60829RanBP2-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5100.
GeneTreeiENSGT00390000018731.
HOGENOMiHOG000008139.
HOVERGENiHBG052659.
InParanoidiP60670.
KOiK14015.
OMAiECITAGM.
OrthoDBiEOG71RXJG.
PhylomeDBiP60670.
TreeFamiTF314173.

Family and domain databases

InterProiIPR007717. NPL4.
IPR024682. Npl4_Ub-like_dom.
IPR007716. NPL4_Zn-bd_put.
IPR016563. PolyUb_recognition_cplx_Npl4.
IPR029071. Ubiquitin-rel_dom.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF05021. NPL4. 1 hit.
PF11543. UN_NPL4. 1 hit.
PF05020. zf-NPL4. 1 hit.
[Graphical view]
PIRSFiPIRSF010052. Polyub_prc_Npl4. 1 hit.
SMARTiSM00547. ZnF_RBZ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P60670-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAESIIIRVQ SPDGVKRITA TKRETAATFL KKVAKEFGFQ NNGFSVYINR
60 70 80 90 100
NKTGEITASS SKSLHLLKIK HGDLLFLFPS SLAGPSSEME TSTSVGLKAF
110 120 130 140 150
GAPNVVEDEI DQYLSKQDGK IYRSRDPQLC RHGPLGKCVH CVPLEPFDED
160 170 180 190 200
YLNHLEPPVK HMSFHAYIRK LTGGADKGKF VALENISCKI KSGCEGHLPW
210 220 230 240 250
PNGICTKCQP SAITLNRQKY RHVDNIMFEN HTVADRFLDF WRKTGNQHFG
260 270 280 290 300
YLYGRYTEHK DIPLGIRAEV AAIYEPPQIG TQNSLELLED PKAEVVDEIA
310 320 330 340 350
AKLGLRKVGW IFTDLVSEDT RKGTVRYSRN KDTYFLSSEE CITAGDFQNK
360 370 380 390 400
HPNICRLSPD GHFGSKFVTA VATGGPDNQV HFEGYQVSNQ CMALVRDECL
410 420 430 440 450
LPCKDAPELG YAKESSSEQY VPDVFYKDID KFGNEITQLA RPLPVEYLII
460 470 480 490 500
DITTTFPKDP VYTFSISQNP FPIENRDVLG ETQDFHSLAT YLSQNTSSVF
510 520 530 540 550
LDTISDFHLL LFLVTNEVMP LQDSISLLLE AVRTRNEELA QTWKKSEQWA
560 570 580 590 600
TIEQLCSTVG VQLPGLHEFG AVGGSARAAT SAMWACQHCT FMNQPGTGHC

EMCSLPRT
Length:608
Mass (Da):68,017
Last modified:January 23, 2007 - v3
Checksum:i378E31B35D559336
GO
Isoform 2 (identifier: P60670-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     452-483: Missing.

Note: No experimental confirmation available.

Show »
Length:576
Mass (Da):64,379
Checksum:i478732FF9B936035
GO

Sequence cautioni

The sequence BAC98185.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei452 – 48332Missing in isoform 2. 1 PublicationVSP_009790Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129375 mRNA. Translation: BAC98185.1. Different initiation.
AL669855 Genomic DNA. Translation: CAM27040.1.
AL669855 Genomic DNA. Translation: CAM27041.1.
BC065156 mRNA. Translation: AAH65156.1.
CCDSiCCDS25732.1. [P60670-2]
CCDS56827.1. [P60670-1]
RefSeqiNP_001181952.1. NM_001195023.1. [P60670-1]
NP_955763.1. NM_199469.2. [P60670-2]
UniGeneiMm.309520.

Genome annotation databases

EnsembliENSMUST00000044271; ENSMUSP00000035851; ENSMUSG00000039703. [P60670-1]
ENSMUST00000103017; ENSMUSP00000099306; ENSMUSG00000039703. [P60670-2]
GeneIDi217365.
KEGGimmu:217365.
UCSCiuc007msp.2. mouse. [P60670-2]
uc007msq.2. mouse. [P60670-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129375 mRNA. Translation: BAC98185.1. Different initiation.
AL669855 Genomic DNA. Translation: CAM27040.1.
AL669855 Genomic DNA. Translation: CAM27041.1.
BC065156 mRNA. Translation: AAH65156.1.
CCDSiCCDS25732.1. [P60670-2]
CCDS56827.1. [P60670-1]
RefSeqiNP_001181952.1. NM_001195023.1. [P60670-1]
NP_955763.1. NM_199469.2. [P60670-2]
UniGeneiMm.309520.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PJHNMR-A1-80[»]
ProteinModelPortaliP60670.
SMRiP60670. Positions 2-77, 578-608.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229901. 5 interactions.
STRINGi10090.ENSMUSP00000035851.

Proteomic databases

MaxQBiP60670.
PaxDbiP60670.
PRIDEiP60670.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044271; ENSMUSP00000035851; ENSMUSG00000039703. [P60670-1]
ENSMUST00000103017; ENSMUSP00000099306; ENSMUSG00000039703. [P60670-2]
GeneIDi217365.
KEGGimmu:217365.
UCSCiuc007msp.2. mouse. [P60670-2]
uc007msq.2. mouse. [P60670-1]

Organism-specific databases

CTDi55666.
MGIiMGI:2679787. Nploc4.
RougeiSearch...

Phylogenomic databases

eggNOGiCOG5100.
GeneTreeiENSGT00390000018731.
HOGENOMiHOG000008139.
HOVERGENiHBG052659.
InParanoidiP60670.
KOiK14015.
OMAiECITAGM.
OrthoDBiEOG71RXJG.
PhylomeDBiP60670.
TreeFamiTF314173.

Enzyme and pathway databases

UniPathwayiUPA00144.

Miscellaneous databases

ChiTaRSiNploc4. mouse.
EvolutionaryTraceiP60670.
NextBioi375815.
PROiP60670.
SOURCEiSearch...

Gene expression databases

BgeeiP60670.
GenevestigatoriP60670.

Family and domain databases

InterProiIPR007717. NPL4.
IPR024682. Npl4_Ub-like_dom.
IPR007716. NPL4_Zn-bd_put.
IPR016563. PolyUb_recognition_cplx_Npl4.
IPR029071. Ubiquitin-rel_dom.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF05021. NPL4. 1 hit.
PF11543. UN_NPL4. 1 hit.
PF05020. zf-NPL4. 1 hit.
[Graphical view]
PIRSFiPIRSF010052. Polyub_prc_Npl4. 1 hit.
SMARTiSM00547. ZnF_RBZ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Embryonic tail.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6.
    Tissue: Brain.
  4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-179, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiNPL4_MOUSE
AccessioniPrimary (citable) accession number: P60670
Secondary accession number(s): B1ATY4, B1ATY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: January 23, 2007
Last modified: March 4, 2015
This is version 100 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.