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Protein

Myosin light polypeptide 6

Gene

MYL6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory light chain of myosin. Does not bind calcium.

GO - Molecular functioni

  • actin-dependent ATPase activity Source: HGNC
  • calcium ion binding Source: InterPro
  • motor activity Source: HGNC
  • structural constituent of muscle Source: HGNC

GO - Biological processi

  • muscle contraction Source: HGNC
  • muscle filament sliding Source: HGNC
  • skeletal muscle tissue development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Muscle protein, Myosin

Enzyme and pathway databases

ReactomeiR-HSA-3928663. EPHA-mediated growth cone collapse.
R-HSA-416572. Sema4D induced cell migration and growth-cone collapse.
R-HSA-445355. Smooth Muscle Contraction.
R-HSA-5625740. RHO GTPases activate PKNs.
R-HSA-5625900. RHO GTPases activate CIT.
R-HSA-5627117. RHO GTPases Activate ROCKs.
R-HSA-5627123. RHO GTPases activate PAKs.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin light polypeptide 6
Alternative name(s):
17 kDa myosin light chain
Short name:
LC17
Myosin light chain 3
Short name:
MLC-3
Myosin light chain alkali 3
Short name:
Myosin light chain A3
Smooth muscle and nonmuscle myosin light chain alkali 6
Gene namesi
Name:MYL6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:7587. MYL6.

Subcellular locationi

GO - Cellular componenti

  • brush border Source: UniProtKB
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • membrane Source: UniProtKB
  • myosin complex Source: HGNC
  • unconventional myosin complex Source: BHF-UCL
  • vesicle Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31384.

Polymorphism and mutation databases

BioMutaiMYL6.
DMDMi47606436.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 151150Myosin light polypeptide 6PRO_0000198690Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylcysteineCombined sources
Modified residuei57 – 571PhosphoserineCombined sources
Modified residuei81 – 811N6-acetyllysineCombined sources
Isoform Smooth muscle (identifier: P60660-2)
Modified residuei135 – 1351PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP60660.
MaxQBiP60660.
PaxDbiP60660.
PeptideAtlasiP60660.
PRIDEiP60660.
TopDownProteomicsiP60660-1. [P60660-1]
P60660-2. [P60660-2]

2D gel databases

DOSAC-COBS-2DPAGEP60660.
SWISS-2DPAGEP60660.

PTM databases

iPTMnetiP60660.
PhosphoSiteiP60660.
SwissPalmiP60660.

Expressioni

Gene expression databases

BgeeiP60660.
CleanExiHS_MYL6.
ExpressionAtlasiP60660. baseline and differential.
GenevisibleiP60660. HS.

Organism-specific databases

HPAiHPA046859.

Interactioni

Subunit structurei

Myosin is a hexamer of 2 heavy chains and 4 light chains. Interacts with SPATA6.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ADAMTS12P583973EBI-300817,EBI-9028051
ESR1P033723EBI-300817,EBI-78473

Protein-protein interaction databases

BioGridi110721. 65 interactions.
IntActiP60660. 42 interactions.
MINTiMINT-1131667.
STRINGi9606.ENSP00000446714.

Structurei

3D structure databases

ProteinModelPortaliP60660.
SMRiP60660. Positions 4-151.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 4236EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini84 – 11936EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini119 – 15133EF-hand 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0030. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00590000082921.
HOVERGENiHBG012180.
InParanoidiP60660.
KOiK12751.
OrthoDBiEOG7HQN9N.
PhylomeDBiP60660.
TreeFamiTF351553.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Non-muscle (identifier: P60660-1) [UniParc]FASTAAdd to basket

Also known as: MLC3nm, LC17A, LC17-nm

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCDFTEDQTA EFKEAFQLFD RTGDGKILYS QCGDVMRALG QNPTNAEVLK
60 70 80 90 100
VLGNPKSDEM NVKVLDFEHF LPMLQTVAKN KDQGTYEDYV EGLRVFDKEG
110 120 130 140 150
NGTVMGAEIR HVLVTLGEKM TEEEVEMLVA GHEDSNGCIN YEAFVRHILS

G
Length:151
Mass (Da):16,930
Last modified:January 23, 2007 - v2
Checksum:iA2B7B4F41179523D
GO
Isoform Smooth muscle (identifier: P60660-2) [UniParc] [UniParc]FASTAAdd to basket

Also known as: MLC3sm, LC17B, LC17-sm

The sequence of this isoform differs from the canonical sequence as follows:
     143-151: AFVRHILSG → ELVRMVLNG

Show »
Length:151
Mass (Da):16,961
Checksum:i25B244E686095233
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 101A → ADLIPST in AAA20643 (PubMed:8188229).Curated
Sequence conflicti45 – 451N → D in AAA59853 (PubMed:2304459).Curated
Sequence conflicti76 – 761T → R in AAA20643 (PubMed:8188229).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti85 – 851T → I.
Corresponds to variant rs11553509 [ dbSNP | Ensembl ].
VAR_050457
Natural varianti103 – 1031T → P.
Corresponds to variant rs1050470 [ dbSNP | Ensembl ].
VAR_034118

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei143 – 1519AFVRHILSG → ELVRMVLNG in isoform Smooth muscle. 2 PublicationsVSP_009735

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22918 mRNA. Translation: AAA36347.1.
M22919 Genomic DNA. Translation: AAA59892.1.
M22919 Genomic DNA. Translation: AAA59893.1.
M22920 mRNA. Translation: AAA36348.1.
M31212 mRNA. Translation: AAA59853.1.
U02629 mRNA. Translation: AAA20643.1.
AB046613 mRNA. Translation: BAB62402.1.
CR457014 mRNA. Translation: CAG33295.1.
BC006781 mRNA. Translation: AAH06781.2.
BC017455 mRNA. Translation: AAH17455.1.
BC071661 mRNA. Translation: AAH71661.1.
BC093066 mRNA. Translation: AAH93066.1.
CCDSiCCDS31834.1. [P60660-2]
CCDS8906.1.
PIRiA33709. MOHU6N.
A49620. MOHU6E.
B33709. MOHU6M.
RefSeqiNP_066299.2. NM_021019.4. [P60660-1]
NP_524147.2. NM_079423.3. [P60660-2]
UniGeneiHs.632717.

Genome annotation databases

EnsembliENST00000547649; ENSP00000446714; ENSG00000092841. [P60660-2]
ENST00000548293; ENSP00000448101; ENSG00000092841. [P60660-1]
ENST00000550697; ENSP00000446955; ENSG00000092841. [P60660-1]
GeneIDi4637.
KEGGihsa:4637.
UCSCiuc001sjw.3. human.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22918 mRNA. Translation: AAA36347.1.
M22919 Genomic DNA. Translation: AAA59892.1.
M22919 Genomic DNA. Translation: AAA59893.1.
M22920 mRNA. Translation: AAA36348.1.
M31212 mRNA. Translation: AAA59853.1.
U02629 mRNA. Translation: AAA20643.1.
AB046613 mRNA. Translation: BAB62402.1.
CR457014 mRNA. Translation: CAG33295.1.
BC006781 mRNA. Translation: AAH06781.2.
BC017455 mRNA. Translation: AAH17455.1.
BC071661 mRNA. Translation: AAH71661.1.
BC093066 mRNA. Translation: AAH93066.1.
CCDSiCCDS31834.1. [P60660-2]
CCDS8906.1.
PIRiA33709. MOHU6N.
A49620. MOHU6E.
B33709. MOHU6M.
RefSeqiNP_066299.2. NM_021019.4. [P60660-1]
NP_524147.2. NM_079423.3. [P60660-2]
UniGeneiHs.632717.

3D structure databases

ProteinModelPortaliP60660.
SMRiP60660. Positions 4-151.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110721. 65 interactions.
IntActiP60660. 42 interactions.
MINTiMINT-1131667.
STRINGi9606.ENSP00000446714.

PTM databases

iPTMnetiP60660.
PhosphoSiteiP60660.
SwissPalmiP60660.

Polymorphism and mutation databases

BioMutaiMYL6.
DMDMi47606436.

2D gel databases

DOSAC-COBS-2DPAGEP60660.
SWISS-2DPAGEP60660.

Proteomic databases

EPDiP60660.
MaxQBiP60660.
PaxDbiP60660.
PeptideAtlasiP60660.
PRIDEiP60660.
TopDownProteomicsiP60660-1. [P60660-1]
P60660-2. [P60660-2]

Protocols and materials databases

DNASUi4637.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000547649; ENSP00000446714; ENSG00000092841. [P60660-2]
ENST00000548293; ENSP00000448101; ENSG00000092841. [P60660-1]
ENST00000550697; ENSP00000446955; ENSG00000092841. [P60660-1]
GeneIDi4637.
KEGGihsa:4637.
UCSCiuc001sjw.3. human.

Organism-specific databases

CTDi4637.
GeneCardsiMYL6.
HGNCiHGNC:7587. MYL6.
HPAiHPA046859.
MIMi609931. gene.
neXtProtiNX_P60660.
PharmGKBiPA31384.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0030. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00590000082921.
HOVERGENiHBG012180.
InParanoidiP60660.
KOiK12751.
OrthoDBiEOG7HQN9N.
PhylomeDBiP60660.
TreeFamiTF351553.

Enzyme and pathway databases

ReactomeiR-HSA-3928663. EPHA-mediated growth cone collapse.
R-HSA-416572. Sema4D induced cell migration and growth-cone collapse.
R-HSA-445355. Smooth Muscle Contraction.
R-HSA-5625740. RHO GTPases activate PKNs.
R-HSA-5625900. RHO GTPases activate CIT.
R-HSA-5627117. RHO GTPases Activate ROCKs.
R-HSA-5627123. RHO GTPases activate PAKs.

Miscellaneous databases

ChiTaRSiMYL6. human.
GeneWikiiMYL6.
GenomeRNAii4637.
PROiP60660.
SOURCEiSearch...

Gene expression databases

BgeeiP60660.
CleanExiHS_MYL6.
ExpressionAtlasiP60660. baseline and differential.
GenevisibleiP60660. HS.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The alkali light chains of human smooth and nonmuscle myosins are encoded by a single gene. Tissue-specific expression by alternative splicing pathways."
    Lenz S., Lohse P., Seidel U., Arnold H.H.
    J. Biol. Chem. 264:9009-9015(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS NON-MUSCLE AND SMOOTH MUSCLE).
  2. "Characterization of human myosin light chains 1sa and 3nm: implications for isoform evolution and function."
    Hailstones D.L., Gunning P.W.
    Mol. Cell. Biol. 10:1095-1104(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM NON-MUSCLE).
  3. "Molecular cloning, sequencing, and characterization of smooth muscle myosin alkali light chain from human eye cDNA: homology with myocardial fatty acid ethyl ester synthase-III cDNA."
    Bora P.S., Bora N.S., Wu X., Kaplan H.J., Lange L.G.
    Genomics 19:186-188(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM NON-MUSCLE).
    Tissue: Eye.
  4. "Molecular cloning and sequencing of myosin light chains in human megakaryoblastic leukemia cells."
    Watanabe M., Kohri M., Takaishi M., Horie R., Higashihara M.
    J. Smooth Muscle Res. 37:25-38(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM NON-MUSCLE).
  5. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-MUSCLE).
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS NON-MUSCLE AND SMOOTH MUSCLE).
    Tissue: Brain, Duodenum, Lung and Placenta.
  7. Bienvenut W.V.
    Submitted (MAR-2005) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 14-21; 38-50; 64-94 AND 111-119, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: B-cell lymphoma.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-135 (ISOFORM SMOOTH MUSCLE), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT CYS-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-81, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT CYS-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT CYS-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "Toward a comprehensive characterization of a human cancer cell phosphoproteome."
    Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., Mohammed S.
    J. Proteome Res. 12:260-271(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-57, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma and Erythroleukemia.
  15. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-57, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  16. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT CYS-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMYL6_HUMAN
AccessioniPrimary (citable) accession number: P60660
Secondary accession number(s): P16475
, P24572, P24573, Q12790, Q561V9, Q6IAZ0, Q6IPY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.