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Protein

Myosin light polypeptide 6

Gene

MYL6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory light chain of myosin. Does not bind calcium.

GO - Molecular functioni

  • actin-dependent ATPase activity Source: HGNC
  • calcium ion binding Source: InterPro
  • motor activity Source: HGNC
  • structural constituent of muscle Source: HGNC

GO - Biological processi

  • muscle contraction Source: HGNC
  • muscle filament sliding Source: HGNC
  • skeletal muscle tissue development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Muscle protein, Myosin

Enzyme and pathway databases

ReactomeiR-HSA-3928663. EPHA-mediated growth cone collapse.
R-HSA-416572. Sema4D induced cell migration and growth-cone collapse.
R-HSA-445355. Smooth Muscle Contraction.
R-HSA-5625740. RHO GTPases activate PKNs.
R-HSA-5625900. RHO GTPases activate CIT.
R-HSA-5627117. RHO GTPases Activate ROCKs.
R-HSA-5627123. RHO GTPases activate PAKs.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin light polypeptide 6
Alternative name(s):
17 kDa myosin light chain
Short name:
LC17
Myosin light chain 3
Short name:
MLC-3
Myosin light chain alkali 3
Short name:
Myosin light chain A3
Smooth muscle and nonmuscle myosin light chain alkali 6
Gene namesi
Name:MYL6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:7587. MYL6.

Subcellular locationi

GO - Cellular componenti

  • brush border Source: UniProtKB
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • extracellular matrix Source: BHF-UCL
  • membrane Source: UniProtKB
  • myosin complex Source: HGNC
  • unconventional myosin complex Source: BHF-UCL
  • vesicle Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000092841.
PharmGKBiPA31384.

Polymorphism and mutation databases

BioMutaiMYL6.
DMDMi47606436.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001986902 – 151Myosin light polypeptide 6Add BLAST150

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylcysteineCombined sources1
Modified residuei57PhosphoserineCombined sources1
Modified residuei81N6-acetyllysineCombined sources1
Isoform Smooth muscle (identifier: P60660-2)
Modified residuei135PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP60660.
MaxQBiP60660.
PaxDbiP60660.
PeptideAtlasiP60660.
PRIDEiP60660.
TopDownProteomicsiP60660-1. [P60660-1]
P60660-2. [P60660-2]

2D gel databases

DOSAC-COBS-2DPAGEP60660.
SWISS-2DPAGEP60660.

PTM databases

iPTMnetiP60660.
PhosphoSitePlusiP60660.
SwissPalmiP60660.

Expressioni

Gene expression databases

BgeeiENSG00000092841.
CleanExiHS_MYL6.
ExpressionAtlasiP60660. baseline and differential.
GenevisibleiP60660. HS.

Organism-specific databases

HPAiHPA046859.

Interactioni

Subunit structurei

Myosin is a hexamer of 2 heavy chains and 4 light chains. Interacts with SPATA6.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ADAMTS12P583973EBI-300817,EBI-9028051
ESR1P033723EBI-300817,EBI-78473
NUDT21O438093EBI-300817,EBI-355720

Protein-protein interaction databases

BioGridi110721. 65 interactors.
IntActiP60660. 43 interactors.
MINTiMINT-1131667.
STRINGi9606.ENSP00000446714.

Structurei

3D structure databases

ProteinModelPortaliP60660.
SMRiP60660.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 42EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini84 – 119EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini119 – 151EF-hand 3PROSITE-ProRule annotationAdd BLAST33

Sequence similaritiesi

Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0030. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00590000082921.
HOVERGENiHBG012180.
InParanoidiP60660.
KOiK12751.
PhylomeDBiP60660.
TreeFamiTF351553.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Non-muscle (identifier: P60660-1) [UniParc]FASTAAdd to basket
Also known as: MLC3nm, LC17A, LC17-nm

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCDFTEDQTA EFKEAFQLFD RTGDGKILYS QCGDVMRALG QNPTNAEVLK
60 70 80 90 100
VLGNPKSDEM NVKVLDFEHF LPMLQTVAKN KDQGTYEDYV EGLRVFDKEG
110 120 130 140 150
NGTVMGAEIR HVLVTLGEKM TEEEVEMLVA GHEDSNGCIN YEAFVRHILS

G
Length:151
Mass (Da):16,930
Last modified:January 23, 2007 - v2
Checksum:iA2B7B4F41179523D
GO
Isoform Smooth muscle (identifier: P60660-2) [UniParc] [UniParc]FASTAAdd to basket
Also known as: MLC3sm, LC17B, LC17-sm

The sequence of this isoform differs from the canonical sequence as follows:
     143-151: AFVRHILSG → ELVRMVLNG

Show »
Length:151
Mass (Da):16,961
Checksum:i25B244E686095233
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10A → ADLIPST in AAA20643 (PubMed:8188229).Curated1
Sequence conflicti45N → D in AAA59853 (PubMed:2304459).Curated1
Sequence conflicti76T → R in AAA20643 (PubMed:8188229).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05045785T → I.Corresponds to variant rs11553509dbSNPEnsembl.1
Natural variantiVAR_034118103T → P.Corresponds to variant rs1050470dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009735143 – 151AFVRHILSG → ELVRMVLNG in isoform Smooth muscle. 2 Publications9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22918 mRNA. Translation: AAA36347.1.
M22919 Genomic DNA. Translation: AAA59892.1.
M22919 Genomic DNA. Translation: AAA59893.1.
M22920 mRNA. Translation: AAA36348.1.
M31212 mRNA. Translation: AAA59853.1.
U02629 mRNA. Translation: AAA20643.1.
AB046613 mRNA. Translation: BAB62402.1.
CR457014 mRNA. Translation: CAG33295.1.
BC006781 mRNA. Translation: AAH06781.2.
BC017455 mRNA. Translation: AAH17455.1.
BC071661 mRNA. Translation: AAH71661.1.
BC093066 mRNA. Translation: AAH93066.1.
CCDSiCCDS31834.1. [P60660-2]
CCDS8906.1.
PIRiA33709. MOHU6N.
A49620. MOHU6E.
B33709. MOHU6M.
RefSeqiNP_066299.2. NM_021019.4. [P60660-1]
NP_524147.2. NM_079423.3. [P60660-2]
UniGeneiHs.632717.

Genome annotation databases

EnsembliENST00000547649; ENSP00000446714; ENSG00000092841. [P60660-2]
ENST00000548293; ENSP00000448101; ENSG00000092841. [P60660-1]
ENST00000550697; ENSP00000446955; ENSG00000092841. [P60660-1]
GeneIDi4637.
KEGGihsa:4637.
UCSCiuc001sjw.3. human.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22918 mRNA. Translation: AAA36347.1.
M22919 Genomic DNA. Translation: AAA59892.1.
M22919 Genomic DNA. Translation: AAA59893.1.
M22920 mRNA. Translation: AAA36348.1.
M31212 mRNA. Translation: AAA59853.1.
U02629 mRNA. Translation: AAA20643.1.
AB046613 mRNA. Translation: BAB62402.1.
CR457014 mRNA. Translation: CAG33295.1.
BC006781 mRNA. Translation: AAH06781.2.
BC017455 mRNA. Translation: AAH17455.1.
BC071661 mRNA. Translation: AAH71661.1.
BC093066 mRNA. Translation: AAH93066.1.
CCDSiCCDS31834.1. [P60660-2]
CCDS8906.1.
PIRiA33709. MOHU6N.
A49620. MOHU6E.
B33709. MOHU6M.
RefSeqiNP_066299.2. NM_021019.4. [P60660-1]
NP_524147.2. NM_079423.3. [P60660-2]
UniGeneiHs.632717.

3D structure databases

ProteinModelPortaliP60660.
SMRiP60660.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110721. 65 interactors.
IntActiP60660. 43 interactors.
MINTiMINT-1131667.
STRINGi9606.ENSP00000446714.

PTM databases

iPTMnetiP60660.
PhosphoSitePlusiP60660.
SwissPalmiP60660.

Polymorphism and mutation databases

BioMutaiMYL6.
DMDMi47606436.

2D gel databases

DOSAC-COBS-2DPAGEP60660.
SWISS-2DPAGEP60660.

Proteomic databases

EPDiP60660.
MaxQBiP60660.
PaxDbiP60660.
PeptideAtlasiP60660.
PRIDEiP60660.
TopDownProteomicsiP60660-1. [P60660-1]
P60660-2. [P60660-2]

Protocols and materials databases

DNASUi4637.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000547649; ENSP00000446714; ENSG00000092841. [P60660-2]
ENST00000548293; ENSP00000448101; ENSG00000092841. [P60660-1]
ENST00000550697; ENSP00000446955; ENSG00000092841. [P60660-1]
GeneIDi4637.
KEGGihsa:4637.
UCSCiuc001sjw.3. human.

Organism-specific databases

CTDi4637.
GeneCardsiMYL6.
HGNCiHGNC:7587. MYL6.
HPAiHPA046859.
MIMi609931. gene.
neXtProtiNX_P60660.
OpenTargetsiENSG00000092841.
PharmGKBiPA31384.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0030. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00590000082921.
HOVERGENiHBG012180.
InParanoidiP60660.
KOiK12751.
PhylomeDBiP60660.
TreeFamiTF351553.

Enzyme and pathway databases

ReactomeiR-HSA-3928663. EPHA-mediated growth cone collapse.
R-HSA-416572. Sema4D induced cell migration and growth-cone collapse.
R-HSA-445355. Smooth Muscle Contraction.
R-HSA-5625740. RHO GTPases activate PKNs.
R-HSA-5625900. RHO GTPases activate CIT.
R-HSA-5627117. RHO GTPases Activate ROCKs.
R-HSA-5627123. RHO GTPases activate PAKs.

Miscellaneous databases

ChiTaRSiMYL6. human.
GeneWikiiMYL6.
GenomeRNAii4637.
PROiP60660.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000092841.
CleanExiHS_MYL6.
ExpressionAtlasiP60660. baseline and differential.
GenevisibleiP60660. HS.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYL6_HUMAN
AccessioniPrimary (citable) accession number: P60660
Secondary accession number(s): P16475
, P24572, P24573, Q12790, Q561V9, Q6IAZ0, Q6IPY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.