P60660 (MYL6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myosin light polypeptide 6

Alternative name(s):
17 kDa myosin light chain
Short name=LC17
Myosin light chain 3
Short name=MLC-3
Myosin light chain alkali 3
Short name=Myosin light chain A3
Smooth muscle and nonmuscle myosin light chain alkali 6

Gene names

Name:

MYL6

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	151 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulatory light chain of myosin. Does not bind calcium.
Subunit structure	Myosin is an hexamer of 2 heavy chains and 4 light chains.
Sequence similarities	Contains 3 EF-hand domains.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
Molecular function	Motor protein Muscle protein Myosin
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	axon guidance Traceable author statement. Source: Reactome muscle filament sliding Traceable author statement Ref.2. Source: HGNC skeletal muscle tissue development Traceable author statement Ref.2. Source: UniProtKB
Cellular component	cytosol Traceable author statement. Source: Reactome unconventional myosin complex Traceable author statement Ref.2. Source: BHF-UCL
Molecular function	actin-dependent ATPase activity Inferred from sequence or structural similarity. Source: HGNC calcium ion binding Inferred from electronic annotation. Source: InterPro motor activity Traceable author statement Ref.2. Source: HGNC protein binding Inferred from physical interaction. Source: IntAct structural constituent of muscle Inferred from direct assay. Source: HGNC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ESR1	P03372	3	EBI-300817,EBI-78473

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Non-muscle (identifier: P60660-1)

Also known as: MLC3nm; LC17A; LC17-nm;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Smooth muscle (identifier: P60660-2)

Also known as: MLC3sm; LC17B; LC17-sm;

The sequence of this isoform differs from the canonical sequence as follows:
     143-151: AFVRHILSG → ELVRMVLNG

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 151	150	Myosin light polypeptide 6		PRO_0000198690
Regions
	Domain	7 – 42	36	EF-hand 1
	Domain	84 – 119	36	EF-hand 2
	Domain	119 – 151	33	EF-hand 3
Amino acid modifications
	Modified residue	2	1	N-acetylcysteine
	Modified residue	29	1	Phosphotyrosine Ref.8
	Modified residue	50	1	N6-acetyllysine Ref.9
	Modified residue	81	1	N6-acetyllysine Ref.9
Natural variations
	Alternative sequence	143 – 151	9	AFVRHILSG → ELVRMVLNG in isoform Smooth muscle.		VSP_009735
	Natural variant	85	1	T → I. Corresponds to variant rs11553509 [ dbSNP | Ensembl ].		VAR_050457
	Natural variant	103	1	T → P. Corresponds to variant rs1050470 [ dbSNP | Ensembl ].		VAR_034118
Experimental info
	Sequence conflict	10	1	A → ADLIPST in AAA20643. Ref.3
	Sequence conflict	45	1	N → D in AAA59853. Ref.2
	Sequence conflict	76	1	T → R in AAA20643. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform Non-muscle (MLC3nm) (LC17A) (LC17-nm) [UniParc]. Last modified January 23, 2007. Version 2. Checksum: A2B7B4F41179523D	FASTA	151	16,930
	10 20 30 40 50 60 MCDFTEDQTA EFKEAFQLFD RTGDGKILYS QCGDVMRALG QNPTNAEVLK VLGNPKSDEM 70 80 90 100 110 120 NVKVLDFEHF LPMLQTVAKN KDQGTYEDYV EGLRVFDKEG NGTVMGAEIR HVLVTLGEKM 130 140 150 TEEEVEMLVA GHEDSNGCIN YEAFVRHILS G
	Isoform Smooth muscle (MLC3sm) (LC17B) (LC17-sm) [UniParc] [UniParc]. Checksum: 25B244E686095233	FASTA	151	16,961
	10 20 30 40 50 60 MCDFTEDQTA EFKEAFQLFD RTGDGKILYS QCGDVMRALG QNPTNAEVLK VLGNPKSDEM 70 80 90 100 110 120 NVKVLDFEHF LPMLQTVAKN KDQGTYEDYV EGLRVFDKEG NGTVMGAEIR HVLVTLGEKM 130 140 150 TEEEVEMLVA GHEDSNGCIN YEELVRMVLN G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The alkali light chains of human smooth and nonmuscle myosins are encoded by a single gene. Tissue-specific expression by alternative splicing pathways." Lenz S., Lohse P., Seidel U., Arnold H.H. J. Biol. Chem. 264:9009-9015(1989) [PubMed: 2722814] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS NON-MUSCLE AND SMOOTH MUSCLE).
[2]	"Characterization of human myosin light chains 1sa and 3nm: implications for isoform evolution and function." Hailstones D.L., Gunning P.W. Mol. Cell. Biol. 10:1095-1104(1990) [PubMed: 2304459] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM NON-MUSCLE).
[3]	"Molecular cloning, sequencing, and characterization of smooth muscle myosin alkali light chain from human eye cDNA: homology with myocardial fatty acid ethyl ester synthase-III cDNA." Bora P.S., Bora N.S., Wu X., Kaplan H.J., Lange L.G. Genomics 19:186-188(1994) [PubMed: 8188229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM NON-MUSCLE). Tissue: Eye.
[4]	"Molecular cloning and sequencing of myosin light chains in human megakaryoblastic leukemia cells." Watanabe M., Kohri M., Takaishi M., Horie R., Higashihara M. J. Smooth Muscle Res. 37:25-38(2001) [PubMed: 11436981] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM NON-MUSCLE).
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-MUSCLE).
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS NON-MUSCLE AND SMOOTH MUSCLE). Tissue: Brain, Duodenum, Lung and Placenta.
[7]	Bienvenut W.V. Submitted (MAR-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 14-21; 38-50; 64-94 AND 111-119, MASS SPECTROMETRY. Tissue: B-cell lymphoma.
[8]	"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J. Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-29, MASS SPECTROMETRY. Tissue: Lung carcinoma.
[9]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-50 AND LYS-81, MASS SPECTROMETRY.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M22918 mRNA. Translation: AAA36347.1. M22919 Genomic DNA. Translation: AAA59892.1. M22919 Genomic DNA. Translation: AAA59893.1. M22920 mRNA. Translation: AAA36348.1. M31212 mRNA. Translation: AAA59853.1. U02629 mRNA. Translation: AAA20643.1. AB046613 mRNA. Translation: BAB62402.1. CR457014 mRNA. Translation: CAG33295.1. BC006781 mRNA. Translation: AAH06781.2. BC017455 mRNA. Translation: AAH17455.1. BC071661 mRNA. Translation: AAH71661.1. BC093066 mRNA. Translation: AAH93066.1.
IPI	IPI00335168. IPI00789605.
PIR	MOHU6N. A33709. MOHU6E. A49620. MOHU6M. B33709.
RefSeq	NP_066299.2. NM_021019.4. NP_524147.2. NM_079423.3.
UniGene	Hs.632717.
3D structure databases
ProteinModelPortal	P60660.
SMR	P60660. Positions 4-151.
ModBase	Search...
Protein-protein interaction databases
IntAct	P60660. 17 interactions.
MINT	MINT-1131667.
STRING	P60660.
PTM databases
PhosphoSite	P60660.
Polymorphism databases
DMDM	47606436.
2D gel databases
SWISS-2DPAGE	P60660.
DOSAC-COBS-2DPAGE	P60660.
Proteomic databases
PRIDE	P60660.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000049160; ENSRNOP00000040759; ENSRNOG00000003892. ENST00000348108; ENSP00000301540; ENSG00000092841.
GeneID	4637.
KEGG	hsa:4637.
UCSC	uc001sjw.1. human.
Organism-specific databases
CTD	4637.
GeneCards	GC12P056552.
H-InvDB	HIX0010720.
HGNC	HGNC:7587. MYL6.
MIM	609931. gene.
neXtProt	NX_P60660.
PharmGKB	PA31384.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG14036.
GeneTree	ENSGT00590000082921.
HOVERGEN	HBG012180.
InParanoid	P60660.
OMA	MNHKLLD.
PhylomeDB	P60660.
Enzyme and pathway databases
Reactome	REACT_111045. Developmental Biology. REACT_17044. Muscle contraction.
Gene expression databases
ArrayExpress	P60660.
Bgee	P60660.
CleanEx	HS_MYL6.
Genevestigator	P60660.
GermOnline	ENSG00000092841. Homo sapiens.
Family and domain databases
InterPro	IPR011992. EF-hand-like_dom. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca-bd. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 2 hits.
KO	K12751.
SMART	SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS50222. EF_HAND_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	17854.
SOURCE	Search...

Entry information

Entry name

MYL6_HUMAN

Accession

Primary (citable) accession number: P60660
Secondary accession number(s): P16475 , P24572, P24573, Q12790, Q561V9, Q6IAZ0, Q6IPY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.