P60654 (SPEB_NEIMB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Agmatinase
EC=3.5.3.11

Alternative name(s):
Agmatine ureohydrolase
Short name=AUH

Gene names

Name:	speB
Ordered Locus Names:	NMB0469

Organism

Neisseria meningitidis serogroup B

Taxonomic identifier

491 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of putrescine from agmatine By similarity. HAMAP MF_01418
Catalytic activity	Agmatine + H2O = putrescine + urea. HAMAP MF_01418
Cofactor	Manganese By similarity. HAMAP MF_01418
Pathway	Amine and polyamine biosynthesis; putrescine biosynthesis via agmatine pathway; putrescine from agmatine: step 1/1. HAMAP MF_01418
Sequence similarities	Belongs to the arginase family. Agmatinase subfamily.

Ontologies

Keywords
Biological process	Polyamine biosynthesis Putrescine biosynthesis Spermidine biosynthesis
Ligand	Manganese Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	putrescine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW spermidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	agmatinase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Agmatinase HAMAP MF_01418		PRO_0000173736
Sites
	Metal binding	128	1	Manganese By similarity
	Metal binding	151	1	Manganese By similarity
	Metal binding	153	1	Manganese By similarity
	Metal binding	155	1	Manganese By similarity
	Metal binding	232	1	Manganese By similarity
	Metal binding	234	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	P60654 [UniParc]. Last modified March 29, 2004. Version 1. Checksum: 1B5013800B47E4BF	FASTA	307	33,905
	10 20 30 40 50 60 MQYSTLAGQT DNSLVSNNFG FLRLPLNFMP YESHADWVIT GVPYDMAVSG RSGARFGPEA 70 80 90 100 110 120 IRRASVNLAW EHRRFPWTFD VRERLNIIDC GDLVFSFGDS RDFVEKMEAH AGKLLSSGKR 130 140 150 160 170 180 CLSLGGDHFI TLPLLRAHAR YFGKLALIHF DAHTDTYDNG SEYDHGTMFY TAPKEGLIDP 190 200 210 220 230 240 SRSVQIGIRT EHSKKLPFTV LSAPKVNEDS VEETVRKIKE TVGNMPVYLT FDIDCLDPSF 250 260 270 280 290 300 APGTGTPVCG GLSSDRALKI LRGLTDLDIV GMDVVEVAPS YDQSDITALA GATIALEMLY LQGAKKD

References

[1]

"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C. Science 287:1809-1815(2000) [PubMed: 10710307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58 / Serogroup B.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE002098 Genomic DNA. Translation: AAF40906.1.
PIR	B81196.
RefSeq	NP_273516.1. NC_003112.2.
3D structure databases
ProteinModelPortal	P60654.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBNEIT00000010609; EBNEIP00000010229; EBNEIG00000010609.
GeneID	902585.
GenomeReviews	Gene locus NMB0469 in contig AE002098_GR.
KEGG	nme:NMB0469.
PATRIC	20356186. VBINeiMen85645_0614.
TIGR	NMB0469.
Phylogenomic databases
GeneTree	EBGT00050000021085.
HOGENOM	HBG391953.
OMA	GPLALIH.
ProtClustDB	PRK02190.
Enzyme and pathway databases
BioCyc	NMEN122586:NMB_0469-MONOMER.
Family and domain databases
HAMAP	MF_01418. SpeB. [Tree]
InterPro	IPR023694. Agmatinase. IPR005925. Agmatinase-rel. IPR006035. Ureohydrolase. IPR023696. Ureohydrolase_domain. IPR020855. Ureohydrolase_Mn_BS. [Graphical view]
Gene3D	G3DSA:3.40.800.10. Ureohydrolase. 1 hit.
KO	K01480.
PANTHER	PTHR11358. Ureohydrolase. 1 hit.
Pfam	PF00491. Arginase. 1 hit. [Graphical view]
PIRSF	PIRSF036979. Arginase. 1 hit.
TIGRFAMs	TIGR01230. Agmatinase. 1 hit.
PROSITE	PS01053. ARGINASE_1. 1 hit. PS51409. ARGINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPEB_NEIMB

Accession

Primary (citable) accession number: P60654
Secondary accession number(s): Q9JRG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
Last sequence update:	March 29, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program