Reviewed, UniProtKB/Swiss-Prot P60651 (SPEB_ECOLI)

Names and origin

Protein names

Recommended name:
    Agmatinase
    EC=3.5.3.11
Alternative name(s):
    Agmatine ureohydrolase
      Short name=AUH

Gene names

Name:	speB
Ordered Locus Names:	b2937, JW2904

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the formation of putrescine from agmatine. HAMAP MF_01418
Catalytic activity	Agmatine + H2O = putrescine + urea. HAMAP MF_01418
Cofactor	Manganese. Ref.5
Enzyme regulation	The expression of auh activity is antagonistically regulated by cyclic AMP and agmatine. In the presence of the cAMP receptor protein, cAMP represses the expression of AUH, while agmatine induces it. HAMAP MF_01418
Pathway	Amine and polyamine biosynthesis; putrescine biosynthesis via agmatine pathway; putrescine from agmatine: step 1/1. HAMAP MF_01418
Sequence similarities	Belongs to the arginase family. Agmatinase subfamily.

Ontologies

Keywords
Biological process	Polyamine biosynthesis Putrescine biosynthesis Spermidine biosynthesis
Ligand	Manganese Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	putrescine biosynthetic process Inferred from electronic annotation. Source: HAMAP spermidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	agmatinase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Agmatinase HAMAP MF_01418		PRO_0000173731
Sites
	Metal binding	126	1	Manganese 1 By similarity
	Metal binding	149	1	Manganese 1 By similarity
	Metal binding	149	1	Manganese 2 By similarity
	Metal binding	151	1	Manganese 2 By similarity
	Metal binding	153	1	Manganese 1 By similarity
	Metal binding	230	1	Manganese 1 By similarity
	Metal binding	230	1	Manganese 2 By similarity
	Metal binding	232	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P60651-1 [UniParc]. Last modified March 29, 2004. Version 1. Checksum: 48208BF2CF155570	FASTA	306	33,557
	10 20 30 40 50 60 MSTLGHQYDN SLVSNAFGFL RLPMNFQPYD SDADWVITGV PFDMATSGRA GGRHGPAAIR 70 80 90 100 110 120 QVSTNLAWEH NRFPWNFDMR ERLNVVDCGD LVYAFGDARE MSEKLQAHAE KLLAAGKRML 130 140 150 160 170 180 SFGGDHFVTL PLLRAHAKHF GKMALVHFDA HTDTYANGCE FDHGTMFYTA PKEGLIDPNH 190 200 210 220 230 240 SVQIGIRTEF DKDNGFTVLD ACQVNDRSVD DVIAQVKQIV GDMPVYLTFD IDCLDPAFAP 250 260 270 280 290 300 GTGTPVIGGL TSDRAIKLVR GLKDLNIVGM DVVEVAPAYD QSEITALAAA TLALEMLYIQ AAKKGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis and sequence of the speB gene encoding agmatine ureohydrolase, a putrescine biosynthetic enzyme in Escherichia coli." Szumanski M.B.W., Boyle S.M. J. Bacteriol. 172:538-547(1990) [PubMed: 2153656] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Nucleotide sequence and analysis of the speA gene encoding biosynthetic arginine decarboxylase in Escherichia coli." Moore R.C., Boyle S.M. J. Bacteriol. 172:4631-4640(1990) [PubMed: 2198270] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-45.
[5]	"Manganese is essential for catalytic activity of Escherichia coli agmatinase." Carvajal N., Lopez V., Salas M., Uribe E., Herrera P., Cerpa J. Biochem. Biophys. Res. Commun. 258:808-811(1999) [PubMed: 10329468] [Abstract] Cited for: COFACTOR.
[6]	"Influence of cyclic AMP, agmatine, and a novel protein encoded by a flanking gene on speB (agmatine ureohydrolase) in Escherichia coli." Szumanski M.B.W., Boyle S.M. J. Bacteriol. 174:758-764(1992) [PubMed: 1310091] [Abstract] Cited for: REGULATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M32363 Genomic DNA. Translation: AAA83909.1. U28377 Genomic DNA. Translation: AAA69104.1. U00096 Genomic DNA. Translation: AAC75974.1. AP009048 Genomic DNA. Translation: BAE77000.1. M31770 Genomic DNA. Translation: AAA24647.1.
PIR	C42604. G85950.
RefSeq	AP_003494.1. NP_417412.1.
3D structure databases
HSSP	HSSP built from PDB template 2CEV based on UniProtKB P53608.
ModBase	Search...
Protein-protein interaction databases
IntAct	P60651. 7 interactions.
STRING	P60651.
2-D gel databases
SWISS-2DPAGE	P60651.
Genome annotation databases
GeneID	947715.
GenomeReviews	Gene locus JW2904 in contig AP009048_GR. Gene locus b2937 in contig U00096_GR.
KEGG	ecj:JW2904. eco:b2937.
Organism-specific databases
EchoBASE	EB0953.
EcoGene	EG10960. speB.
CMR	Search...
Phylogenomic databases
HOGENOM	P60651.
OMA	CAQITAL.
Enzyme and pathway databases
BioCyc	EcoCyc:AGMATIN-MON. MetaCyc:AGMATIN-MON.
Gene expression databases
Genevestigator	P60651.
Family and domain databases
HAMAP	MF_01418. [Tree]
InterPro	IPR005925. Agmatinase. IPR006035. Ureohydrolase. [Graphical view]
Gene3D	G3DSA:3.40.800.10. Ureohydrolase. 1 hit.
PANTHER	PTHR11358. Ureohydrolase. 1 hit.
Pfam	PF00491. Arginase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01230. agmatinase. 1 hit.
PROSITE	PS01053. ARGINASE_1. 1 hit. PS51409. ARGINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPEB_ECOLI

Accession

Primary (citable) accession number: P60651
Secondary accession number(s): P16936, Q2M9Q6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
Last sequence update:	March 29, 2004
Last modified:	November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)