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P60651

- SPEB_ECOLI

UniProt

P60651 - SPEB_ECOLI

Protein

Agmatinase

Gene

speB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (29 Mar 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of putrescine from agmatine.1 Publication

    Catalytic activityi

    Agmatine + H2O = putrescine + urea.

    Cofactori

    Manganese.1 Publication

    Enzyme regulationi

    The expression of auh activity is antagonistically regulated by cyclic AMP and agmatine. In the presence of the cAMP receptor protein, cAMP represses the expression of AUH, while agmatine induces it.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi126 – 1261Manganese 1By similarity
    Metal bindingi149 – 1491Manganese 1By similarity
    Metal bindingi149 – 1491Manganese 2By similarity
    Metal bindingi151 – 1511Manganese 2By similarity
    Metal bindingi153 – 1531Manganese 1By similarity
    Sitei163 – 1631Important for catalytic activity
    Metal bindingi230 – 2301Manganese 1By similarity
    Metal bindingi230 – 2301Manganese 2By similarity
    Metal bindingi232 – 2321Manganese 2By similarity

    GO - Molecular functioni

    1. agmatinase activity Source: EcoCyc
    2. manganese ion binding Source: EcoCyc

    GO - Biological processi

    1. putrescine biosynthetic process Source: EcoCyc
    2. putrescine biosynthetic process from arginine Source: UniProtKB-UniPathway
    3. spermidine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Polyamine biosynthesis, Putrescine biosynthesis, Spermidine biosynthesis

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:AGMATIN-MONOMER.
    ECOL316407:JW2904-MONOMER.
    MetaCyc:AGMATIN-MONOMER.
    UniPathwayiUPA00534; UER00287.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Agmatinase (EC:3.5.3.11)
    Alternative name(s):
    Agmatine ureohydrolase
    Short name:
    AUH
    Gene namesi
    Name:speB
    Ordered Locus Names:b2937, JW2904
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10960. speB.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi163 – 1631H → F: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 306306AgmatinasePRO_0000173731Add
    BLAST

    Proteomic databases

    PaxDbiP60651.
    PRIDEiP60651.

    2D gel databases

    SWISS-2DPAGEP60651.

    Expressioni

    Gene expression databases

    GenevestigatoriP60651.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-10906N.
    IntActiP60651. 7 interactions.
    STRINGi511145.b2937.

    Structurei

    3D structure databases

    ProteinModelPortaliP60651.
    SMRiP60651. Positions 41-305.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the arginase family. Agmatinase subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0010.
    HOGENOMiHOG000204320.
    KOiK01480.
    OMAiKPDYSLY.
    OrthoDBiEOG6R2GW5.
    PhylomeDBiP60651.

    Family and domain databases

    Gene3Di3.40.800.10. 1 hit.
    HAMAPiMF_01418. SpeB.
    InterProiIPR023694. Agmatinase.
    IPR005925. Agmatinase-rel.
    IPR006035. Ureohydrolase.
    IPR023696. Ureohydrolase_domain.
    IPR020855. Ureohydrolase_Mn_BS.
    [Graphical view]
    PANTHERiPTHR11358. PTHR11358. 1 hit.
    PfamiPF00491. Arginase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036979. Arginase. 1 hit.
    TIGRFAMsiTIGR01230. agmatinase. 1 hit.
    PROSITEiPS01053. ARGINASE_1. 1 hit.
    PS51409. ARGINASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P60651-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTLGHQYDN SLVSNAFGFL RLPMNFQPYD SDADWVITGV PFDMATSGRA    50
    GGRHGPAAIR QVSTNLAWEH NRFPWNFDMR ERLNVVDCGD LVYAFGDARE 100
    MSEKLQAHAE KLLAAGKRML SFGGDHFVTL PLLRAHAKHF GKMALVHFDA 150
    HTDTYANGCE FDHGTMFYTA PKEGLIDPNH SVQIGIRTEF DKDNGFTVLD 200
    ACQVNDRSVD DVIAQVKQIV GDMPVYLTFD IDCLDPAFAP GTGTPVIGGL 250
    TSDRAIKLVR GLKDLNIVGM DVVEVAPAYD QSEITALAAA TLALEMLYIQ 300
    AAKKGE 306
    Length:306
    Mass (Da):33,557
    Last modified:March 29, 2004 - v1
    Checksum:i48208BF2CF155570
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M32363 Genomic DNA. Translation: AAA83909.1.
    U28377 Genomic DNA. Translation: AAA69104.1.
    U00096 Genomic DNA. Translation: AAC75974.1.
    AP009048 Genomic DNA. Translation: BAE77000.1.
    M31770 Genomic DNA. Translation: AAA24647.1.
    PIRiC42604.
    G85950.
    RefSeqiNP_417412.1. NC_000913.3.
    YP_491136.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75974; AAC75974; b2937.
    BAE77000; BAE77000; BAE77000.
    GeneIDi12931916.
    947715.
    KEGGiecj:Y75_p2867.
    eco:b2937.
    PATRICi32121286. VBIEscCol129921_3031.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M32363 Genomic DNA. Translation: AAA83909.1 .
    U28377 Genomic DNA. Translation: AAA69104.1 .
    U00096 Genomic DNA. Translation: AAC75974.1 .
    AP009048 Genomic DNA. Translation: BAE77000.1 .
    M31770 Genomic DNA. Translation: AAA24647.1 .
    PIRi C42604.
    G85950.
    RefSeqi NP_417412.1. NC_000913.3.
    YP_491136.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P60651.
    SMRi P60651. Positions 41-305.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-10906N.
    IntActi P60651. 7 interactions.
    STRINGi 511145.b2937.

    2D gel databases

    SWISS-2DPAGE P60651.

    Proteomic databases

    PaxDbi P60651.
    PRIDEi P60651.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75974 ; AAC75974 ; b2937 .
    BAE77000 ; BAE77000 ; BAE77000 .
    GeneIDi 12931916.
    947715.
    KEGGi ecj:Y75_p2867.
    eco:b2937.
    PATRICi 32121286. VBIEscCol129921_3031.

    Organism-specific databases

    EchoBASEi EB0953.
    EcoGenei EG10960. speB.

    Phylogenomic databases

    eggNOGi COG0010.
    HOGENOMi HOG000204320.
    KOi K01480.
    OMAi KPDYSLY.
    OrthoDBi EOG6R2GW5.
    PhylomeDBi P60651.

    Enzyme and pathway databases

    UniPathwayi UPA00534 ; UER00287 .
    BioCyci EcoCyc:AGMATIN-MONOMER.
    ECOL316407:JW2904-MONOMER.
    MetaCyc:AGMATIN-MONOMER.

    Miscellaneous databases

    PROi P60651.

    Gene expression databases

    Genevestigatori P60651.

    Family and domain databases

    Gene3Di 3.40.800.10. 1 hit.
    HAMAPi MF_01418. SpeB.
    InterProi IPR023694. Agmatinase.
    IPR005925. Agmatinase-rel.
    IPR006035. Ureohydrolase.
    IPR023696. Ureohydrolase_domain.
    IPR020855. Ureohydrolase_Mn_BS.
    [Graphical view ]
    PANTHERi PTHR11358. PTHR11358. 1 hit.
    Pfami PF00491. Arginase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036979. Arginase. 1 hit.
    TIGRFAMsi TIGR01230. agmatinase. 1 hit.
    PROSITEi PS01053. ARGINASE_1. 1 hit.
    PS51409. ARGINASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis and sequence of the speB gene encoding agmatine ureohydrolase, a putrescine biosynthetic enzyme in Escherichia coli."
      Szumanski M.B.W., Boyle S.M.
      J. Bacteriol. 172:538-547(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Nucleotide sequence and analysis of the speA gene encoding biosynthetic arginine decarboxylase in Escherichia coli."
      Moore R.C., Boyle S.M.
      J. Bacteriol. 172:4631-4640(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-45.
    5. "Manganese is essential for catalytic activity of Escherichia coli agmatinase."
      Carvajal N., Lopez V., Salas M., Uribe E., Herrera P., Cerpa J.
      Biochem. Biophys. Res. Commun. 258:808-811(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: COFACTOR.
    6. "Evidence that histidine-163 is critical for catalytic activity, but not for substrate binding to Escherichia coli agmatinase."
      Carvajal N., Olate J., Salas M., Lopez V., Cerpa J., Herrera P., Uribe E.
      Biochem. Biophys. Res. Commun. 264:196-200(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF HIS-163.
    7. "Influence of cyclic AMP, agmatine, and a novel protein encoded by a flanking gene on speB (agmatine ureohydrolase) in Escherichia coli."
      Szumanski M.B.W., Boyle S.M.
      J. Bacteriol. 174:758-764(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: REGULATION.

    Entry informationi

    Entry nameiSPEB_ECOLI
    AccessioniPrimary (citable) accession number: P60651
    Secondary accession number(s): P16936, Q2M9Q6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2004
    Last sequence update: March 29, 2004
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3