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Protein

Agmatinase

Gene

speB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of putrescine from agmatine.1 Publication

Catalytic activityi

Agmatine + H2O = putrescine + urea.

Cofactori

Mn2+1 Publication

Enzyme regulationi

The expression of auh activity is antagonistically regulated by cyclic AMP and agmatine. In the presence of the cAMP receptor protein, cAMP represses the expression of AUH, while agmatine induces it.

Pathwayi: putrescine biosynthesis via agmatine pathway

This protein is involved in step 1 of the subpathway that synthesizes putrescine from agmatine.
Proteins known to be involved in this subpathway in this organism are:
  1. Agmatinase (speB)
This subpathway is part of the pathway putrescine biosynthesis via agmatine pathway, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes putrescine from agmatine, the pathway putrescine biosynthesis via agmatine pathway and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi126Manganese 1By similarity1
Metal bindingi149Manganese 1By similarity1
Metal bindingi149Manganese 2By similarity1
Metal bindingi151Manganese 2By similarity1
Metal bindingi153Manganese 1By similarity1
Sitei163Important for catalytic activity1
Metal bindingi230Manganese 1By similarity1
Metal bindingi230Manganese 2By similarity1
Metal bindingi232Manganese 2By similarity1

GO - Molecular functioni

  • agmatinase activity Source: EcoCyc
  • manganese ion binding Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processPolyamine biosynthesis, Putrescine biosynthesis, Spermidine biosynthesis
LigandManganese, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:AGMATIN-MONOMER
MetaCyc:AGMATIN-MONOMER
UniPathwayiUPA00534; UER00287

Names & Taxonomyi

Protein namesi
Recommended name:
Agmatinase (EC:3.5.3.11)
Alternative name(s):
Agmatine ureohydrolase
Short name:
AUH
Gene namesi
Name:speB
Ordered Locus Names:b2937, JW2904
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10960 speB

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi163H → F: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001737311 – 306AgmatinaseAdd BLAST306

Proteomic databases

EPDiP60651
PaxDbiP60651
PRIDEiP60651

2D gel databases

SWISS-2DPAGEP60651

Interactioni

Protein-protein interaction databases

BioGridi4260910, 40 interactors
DIPiDIP-10906N
IntActiP60651, 7 interactors
STRINGi316385.ECDH10B_3112

Structurei

3D structure databases

ProteinModelPortaliP60651
SMRiP60651
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the arginase family. Agmatinase subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105CYW Bacteria
COG0010 LUCA
HOGENOMiHOG000204320
InParanoidiP60651
KOiK01480
OMAiDTYFGAE
PhylomeDBiP60651

Family and domain databases

HAMAPiMF_01418 SpeB, 1 hit
InterProiView protein in InterPro
IPR023694 Agmatinase
IPR005925 Agmatinase-rel
IPR006035 Ureohydrolase
IPR023696 Ureohydrolase_dom_sf
IPR020855 Ureohydrolase_Mn_BS
PfamiView protein in Pfam
PF00491 Arginase, 1 hit
PIRSFiPIRSF036979 Arginase, 1 hit
SUPFAMiSSF52768 SSF52768, 1 hit
TIGRFAMsiTIGR01230 agmatinase, 1 hit
PROSITEiView protein in PROSITE
PS01053 ARGINASE_1, 1 hit
PS51409 ARGINASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P60651-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTLGHQYDN SLVSNAFGFL RLPMNFQPYD SDADWVITGV PFDMATSGRA
60 70 80 90 100
GGRHGPAAIR QVSTNLAWEH NRFPWNFDMR ERLNVVDCGD LVYAFGDARE
110 120 130 140 150
MSEKLQAHAE KLLAAGKRML SFGGDHFVTL PLLRAHAKHF GKMALVHFDA
160 170 180 190 200
HTDTYANGCE FDHGTMFYTA PKEGLIDPNH SVQIGIRTEF DKDNGFTVLD
210 220 230 240 250
ACQVNDRSVD DVIAQVKQIV GDMPVYLTFD IDCLDPAFAP GTGTPVIGGL
260 270 280 290 300
TSDRAIKLVR GLKDLNIVGM DVVEVAPAYD QSEITALAAA TLALEMLYIQ

AAKKGE
Length:306
Mass (Da):33,557
Last modified:March 29, 2004 - v1
Checksum:i48208BF2CF155570
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32363 Genomic DNA Translation: AAA83909.1
U28377 Genomic DNA Translation: AAA69104.1
U00096 Genomic DNA Translation: AAC75974.1
AP009048 Genomic DNA Translation: BAE77000.1
M31770 Genomic DNA Translation: AAA24647.1
PIRiC42604
G85950
RefSeqiNP_417412.1, NC_000913.3
WP_000105566.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75974; AAC75974; b2937
BAE77000; BAE77000; BAE77000
GeneIDi947715
KEGGiecj:JW2904
eco:b2937
PATRICifig|1411691.4.peg.3796

Similar proteinsi

Entry informationi

Entry nameiSPEB_ECOLI
AccessioniPrimary (citable) accession number: P60651
Secondary accession number(s): P16936, Q2M9Q6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: March 29, 2004
Last modified: March 28, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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