50S ribosomal protein L24 - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P60624 (RL24_ECOLI)

Last modified June 16, 2009. Version 66. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
50S ribosomal protein L24

Gene names

Name:	rplX
Ordered Locus Names:	b3309, JW3271

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	104 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	One of two assembly inititator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit. It is not thought to be involved in the functions of the mature 50S subunit in vitro. Ref.6 One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit. Ref.6
Subunit structure	Part of the 50S ribosomal subunit. Ref.6 Ref.8
Sequence similarities	Belongs to the ribosomal protein L24P family.
Mass spectrometry	Molecular mass is 11186.5 Da from positions 2 - 104. Determined by MALDI. Ref.9

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Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction. Source: IntAct rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
phoB	P0AFJ5	1	EBI-546481,EBI-1116564

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.4

Chain

2 – 104

103

50S ribosomal protein L24 HAMAP MF_01326

PRO_0000130654

Secondary structure

104

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P60624-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 0ABF7DD305FA07F5
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FASTA

104

11,316

        10         20         30         40         50         60 
MAAKIRRDDE VIVLTGKDKG KRGKVKNVLS SGKVIVEGIN LVKKHQKPVP ALNQPGGIVE 

        70         80         90        100 
KEAAIQVSNV AIFNAATGKA DRVGFRFEDG KKVRFFKSNS ETIK

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene." Cerretti D.P., Dean D., Davis G.R., Bedwell D.M., Nomura M. Nucleic Acids Res. 11:2599-2616(1983) [PubMed: 6222285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Primary structure of protein L24 from the Escherichia coli ribosome." Wittmann-Liebold B. FEBS Lett. 108:75-80(1979) [PubMed: 391595] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-104. Strain: K12.
[5]	"Translational regulation by ribosomal protein S8 in Escherichia coli: structural homology between rRNA binding site and feedback target on mRNA." Olins P.O., Nomura M. Nucleic Acids Res. 9:1757-1764(1981) [PubMed: 6262737] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 86-104. Strain: K12 / JM105 / ATCC 47016.
[6]	"The ribosomal protein L24 of Escherichia coli is an assembly protein." Spillmann S., Nierhaus K.H. J. Biol. Chem. 253:7047-7050(1978) [PubMed: 357435] [Abstract] Cited for: FUNCTION IN SUBUNIT ASSEMBLY. Strain: K12 / A19.
[7]	"Initiator proteins for the assembly of the 50S subunit from Escherichia coli ribosomes." Nowotny V., Nierhaus K.H. Proc. Natl. Acad. Sci. U.S.A. 79:7238-7242(1982) [PubMed: 6760192] [Abstract] Cited for: IDENTIFICATION AS AN ASSEMBLY INITIATOR PROTEIN.
[8]	"Incorporation of six additional proteins to complete the assembly map of the 50 S subunit from Escherichia coli ribosomes." Herold M., Nierhaus K.H. J. Biol. Chem. 262:8826-8833(1987) [PubMed: 3298242] [Abstract] Cited for: ASSEMBLY MAP OF THE 50S SUBUNIT. Strain: K12.
[9]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[10]	"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement." Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J. Cell 113:789-801(2003) [PubMed: 12809609] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS). Strain: MRE-600.
[11]	"Structures of the bacterial ribosome at 3.5 A resolution." Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D. Science 310:827-834(2005) [PubMed: 16272117] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES. Strain: MRE-600.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

X01563 Genomic DNA. Translation: CAA25716.1.
U18997 Genomic DNA. Translation: AAA58106.1.
U00096 Genomic DNA. Translation: AAC76334.1.
AP009048 Genomic DNA. Translation: BAE77982.1.
M10195 Genomic DNA. Translation: AAA24050.1.

PIR

R5EC24. H65123.

RefSeq

AP_004481.1.
NP_417768.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1P85	electron microscopy	12.30	S	2-104	[»]
1P86	electron microscopy	11.50	S	2-104	[»]
1VS6	X-ray	3.46	U	1-104	[»]
1VS8	X-ray	3.46	U	1-104	[»]
2AW4	X-ray	3.46	U	2-104	[»]
2AWB	X-ray	3.46	U	2-104	[»]
2GYA	electron microscopy	2.00	S	4-101	[»]
2GYC	electron microscopy	2.00	S	4-102	[»]
2I2T	X-ray	3.22	U	2-103	[»]
2I2V	X-ray	3.22	U	2-103	[»]
2J28	electron microscopy	8.00	U	2-102	[»]
2QAM	X-ray	3.21	U	2-104	[»]
2QAO	X-ray	3.21	U	2-104	[»]
2QBA	X-ray	3.54	U	2-104	[»]
2QBC	X-ray	3.54	U	2-104	[»]
2QBE	X-ray	3.30	U	2-104	[»]
2QBG	X-ray	3.30	U	2-104	[»]
2QBI	X-ray	4.00	U	2-104	[»]
2QBK	X-ray	4.00	U	2-104	[»]
2QOV	X-ray	3.93	U	2-104	[»]
2QOX	X-ray	3.93	U	2-104	[»]
2QOZ	X-ray	3.50	U	2-104	[»]
2QP1	X-ray	3.50	U	2-104	[»]
2RDO	electron microscopy	9.10	U	2-104	[»]
2VHM	X-ray	3.74	U	2-104	[»]
2VHN	X-ray	3.74	U	2-104	[»]
2VRH	electron microscopy	19.00	C	2-104	[»]
2Z4L	X-ray	4.45	U	2-104	[»]
2Z4N	X-ray	4.45	U	2-104	[»]
3BBX	electron microscopy	10.00	U	2-104	[»]
3DF2	X-ray	3.50	U	2-103	[»]
3DF4	X-ray	3.50	U	2-103	[»]
3FIK	electron microscopy	-	U	2-103	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P60624. 51 interactions.

Genome annotation databases

GeneID

947810.

GenomeReviews

Gene locus JW3271 in contig AP009048_GR.
Gene locus b3309 in contig U00096_GR.

KEGG

ecj:JW3271.
eco:b3309.

Organism-specific databases

EchoBASE

EB0877.

EcoGene

EG10884. rplX.

CMR

Search...

Phylogenomic databases

HOGENOM

P60624.

OMA

P60624. KPTRVGY.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10884-MON.

Family and domain databases

HAMAP

MF_01326.
[Tree]

InterPro

IPR005824. KOW.
IPR003256. Ribosomal_L24.
IPR005825. Ribosomal_L24/26_CS.
[Graphical view]

PANTHER

PTHR12903. Ribosomal_L24. 1 hit.

Pfam

PF00467. KOW. 1 hit.
[Graphical view]

ProDom

PD001677. Ribosomal_L24. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00739. KOW. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01079. rplX_bact. 1 hit.

PROSITE

PS01108. RIBOSOMAL_L24. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

RL24_ECOLI

Accession

Primary (citable) accession number: P60624
Secondary accession number(s): P02425, P37438, Q2M6X4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Ribosomal proteins

Ribosomal proteins families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents