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Protein

Cysteine-rich venom protein

Gene
N/A
Organism
Trimeresurus stejnegeri (Chinese green tree viper) (Viridovipera stejnegeri)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Blocks contraction of smooth muscle elicited by high potassium-induced depolarization, but does not block caffein-stimulated contraction. May target voltage-gated calcium channels on smooth muscle (By similarity).By similarity

Keywords - Molecular functioni

Calcium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine-rich venom protein
Short name:
CRVP
Alternative name(s):
Cysteine-rich secretory protein
Stecrisp
OrganismiTrimeresurus stejnegeri (Chinese green tree viper) (Viridovipera stejnegeri)
Taxonomic identifieri39682 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeTrimeresurus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei‹1 – 12›12Sequence analysisAdd
BLAST
Chaini13 – 233221Cysteine-rich venom proteinPRO_0000006290Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi68 ↔ 146PROSITE-ProRule annotation1 Publication
Disulfide bondi85 ↔ 160PROSITE-ProRule annotation1 Publication
Disulfide bondi141 ↔ 157PROSITE-ProRule annotation1 Publication
Disulfide bondi179 ↔ 186PROSITE-ProRule annotation1 Publication
Disulfide bondi182 ↔ 191PROSITE-ProRule annotation1 Publication
Disulfide bondi195 ↔ 228PROSITE-ProRule annotation1 Publication
Disulfide bondi204 ↔ 222PROSITE-ProRule annotation1 Publication
Disulfide bondi213 ↔ 226PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

1
233
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi17 – 193Combined sources
Helixi24 – 3916Combined sources
Beta strandi45 – 473Combined sources
Helixi55 – 6511Combined sources
Turni66 – 683Combined sources
Helixi75 – 784Combined sources
Beta strandi86 – 949Combined sources
Helixi98 – 1069Combined sources
Helixi107 – 1115Combined sources
Turni114 – 1163Combined sources
Beta strandi117 – 1204Combined sources
Helixi126 – 1316Combined sources
Beta strandi138 – 1458Combined sources
Beta strandi149 – 16113Combined sources
Beta strandi175 – 1773Combined sources
Turni178 – 1814Combined sources
Beta strandi185 – 1873Combined sources
Helixi204 – 2096Combined sources
Helixi216 – 2216Combined sources
Helixi223 – 2275Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RC9X-ray1.60A13-233[»]
ProteinModelPortaliP60623.
SMRiP60623. Positions 13-233.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60623.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 159129SCPAdd
BLAST
Domaini195 – 22834ShKTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the CRISP family.Curated
Contains 1 SCP domain.Curated
Contains 1 ShKT domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG004184.

Family and domain databases

Gene3Di3.40.33.10. 1 hit.
InterProiIPR001283. Allrgn_V5/Tpx1.
IPR018244. Allrgn_V5/Tpx1_CS.
IPR014044. CAP_domain.
IPR013871. Cysteine_rich_secretory.
IPR003582. ShKT_dom.
IPR002413. V5_allergen.
[Graphical view]
PANTHERiPTHR10334. PTHR10334. 1 hit.
PfamiPF00188. CAP. 1 hit.
PF08562. Crisp. 1 hit.
[Graphical view]
PRINTSiPR00838. V5ALLERGEN.
PR00837. V5TPXLIKE.
SMARTiSM00198. SCP. 1 hit.
[Graphical view]
SUPFAMiSSF55797. SSF55797. 1 hit.
PROSITEiPS01009. CRISP_1. 1 hit.
PS01010. CRISP_2. 1 hit.
PS51670. SHKT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60623-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
PILAAVLQQS SGNVDFDSES PRKPEIQNEI VDLHNSLRRS VNPTASNMLR
60 70 80 90 100
MEWYPEAADN AERWAYRCIE SHSSYESRVI EGIKCGENIY MSPYPMKWTD
110 120 130 140 150
IIHAWHDEYK DFKYGVGADP PNAVTGHYTQ IVWYKSYRIG CAAAYCPSSP
160 170 180 190 200
YSYFFVCQYC PAGNFIGKTA TPYTSGTPCG DCPSDCDNGL CTNPCTRENK
210 220 230
FTNCNTMVQQ SSCQDNYMKT NCPASCFCQN KII
Length:233
Mass (Da):26,294
Last modified:March 15, 2004 - v1
Checksum:iA9E326F5242DD249
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY423708 mRNA. Translation: AAQ98964.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY423708 mRNA. Translation: AAQ98964.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RC9X-ray1.60A13-233[»]
ProteinModelPortaliP60623.
SMRiP60623. Positions 13-233.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG004184.

Miscellaneous databases

EvolutionaryTraceiP60623.

Family and domain databases

Gene3Di3.40.33.10. 1 hit.
InterProiIPR001283. Allrgn_V5/Tpx1.
IPR018244. Allrgn_V5/Tpx1_CS.
IPR014044. CAP_domain.
IPR013871. Cysteine_rich_secretory.
IPR003582. ShKT_dom.
IPR002413. V5_allergen.
[Graphical view]
PANTHERiPTHR10334. PTHR10334. 1 hit.
PfamiPF00188. CAP. 1 hit.
PF08562. Crisp. 1 hit.
[Graphical view]
PRINTSiPR00838. V5ALLERGEN.
PR00837. V5TPXLIKE.
SMARTiSM00198. SCP. 1 hit.
[Graphical view]
SUPFAMiSSF55797. SSF55797. 1 hit.
PROSITEiPS01009. CRISP_1. 1 hit.
PS01010. CRISP_2. 1 hit.
PS51670. SHKT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Purification and molecular cloning of a novel snake venom protein that belongs to CRISP family."
    Guo M., Teng M.-K., Niu L.-W.
    Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Venom gland.
  2. "Crystal structure of the cysteine-rich secretory protein stecrisp reveals that the cysteine-rich domain has a K+ channel inhibitor-like fold."
    Guo M., Teng M.-K., Niu L.-W., Liu Q., Huang Q., Hao Q.
    J. Biol. Chem. 280:12405-12412(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 13-233, DISULFIDE BONDS.
    Tissue: Venom.

Entry informationi

Entry nameiCRVP_TRIST
AccessioniPrimary (citable) accession number: P60623
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: March 15, 2004
Last modified: May 11, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.