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P60617 (RL10E_HALMA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
50S ribosomal protein L10e
Gene names
Name:rpl10e
Ordered Locus Names:rrnAC2357
OrganismHaloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui) [Complete proteome] [HAMAP]
Taxonomic identifier272569 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloarcula

Protein attributes

Sequence length177 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This is 1 of 5 proteins that mediate the attachment of the 5S rRNA onto the large ribosomal subunit, stabilizing the orientation of adjacent RNA domains. Modeling places the A and P site tRNAs in close proximity to this protein. HAMAP-Rule MF_00448

Subunit structure

Part of the 50S ribosomal subunit. Weakly binds 5S rRNA. Probably binds the A and P site tRNAs. Ref.2 Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10

Sequence similarities

Belongs to the ribosomal protein L10e family.

Ontologies

Keywords
   LigandRNA-binding
rRNA-binding
tRNA-binding
   Molecular functionRibonucleoprotein
Ribosomal protein
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological_processtranslation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentribosome

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionrRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

structural constituent of ribosome

Inferred from electronic annotation. Source: InterPro

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 17717750S ribosomal protein L10e HAMAP-Rule MF_00448
PRO_0000147132

Secondary structure

................................... 177
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P60617 [UniParc].

Last modified January 4, 2005. Version 2.
Checksum: E7A72FE3683D2BDB

FASTA17719,908
        10         20         30         40         50         60 
MSDKPASMYR DIDKPAYTRR EYITGIPGSK IAQHKMGRKQ KDADDYPVQI SLIVEETVQL 

        70         80         90        100        110        120 
RHGSLEASRL SANRHLIKEL GEEGDYKMTL RKFPHQVLRE NKQATGAGAD RVSDGMRAAF 

       130        140        150        160        170 
GKIVGTAARV QAGEQLFTAY CNVEDAEHVK EAFRRAYNKI TPSCRIKVER GEELLIA 

« Hide

References

« Hide 'large scale' references
[1]"Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea."
Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W., Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E., Hood L., Ng W.V.
Genome Res. 14:2221-2234(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
[2]"The complete atomic structure of the large ribosomal subunit at 2.4 A resolution."
Ban N., Nissen P., Hansen J., Moore P.B., Steitz T.A.
Science 289:905-920(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT.
Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
[3]"The structural basis of ribosome activity in peptide bond synthesis."
Nissen P., Hansen J., Ban N., Moore P.B., Steitz T.A.
Science 289:920-930(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT.
Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
[4]"A pre-translocational intermediate in protein synthesis observed in crystals of enzymatically active 50S subunits."
Schmeing T.M., Seila A.C., Hansen J.L., Freeborn B., Soukup J.K., Scaringe S.A., Strobel S.A., Moore P.B., Steitz T.A.
Nat. Struct. Biol. 9:225-230(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF THE 50S SUBUNIT.
Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
[5]"The kink-turn: a new RNA secondary structure motif."
Klein D.J., Schmeing T.M., Moore P.B., Steitz T.A.
EMBO J. 20:4214-4221(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT.
Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
[6]"The structures of four macrolide antibiotics bound to the large ribosomal subunit."
Hansen J.L., Ippolito J.A., Ban N., Nissen P., Moore P.B., Steitz T.A.
Mol. Cell 10:117-128(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT IN COMPLEX WITH FOUR MACROLIDE ANTIBIOTICS.
Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
[7]"Structural insights into peptide bond formation."
Hansen J.L., Schmeing T.M., Moore P.B., Steitz T.A.
Proc. Natl. Acad. Sci. U.S.A. 99:11670-11675(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF THE 50S SUBUNIT.
Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
[8]"Structures of five antibiotics bound at the peptidyl transferase center of the large ribosomal subunit."
Hansen J.L., Moore P.B., Steitz T.A.
J. Mol. Biol. 330:1061-1075(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT IN COMPLEX WITH FIVE ANTIBIOTICS AT THE PEPTIDYL TRANSFERASE CENTER.
Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
[9]"Structures of deacylated tRNA mimics bound to the E site of the large ribosomal subunit."
Schmeing T.M., Moore P.B., Steitz T.A.
RNA 9:1345-1352(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF THE 50S SUBUNIT WITH TWO DIFFERENT E SITE SUBSTRATES.
[10]"Revisiting the Haloarcula marismortui 50S ribosomal subunit model."
Gabdulkhakov A., Nikonov S., Garber M.
Acta Crystallogr. D 69:997-1004(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY596297 Genomic DNA. Translation: AAV47175.1.
RefSeqYP_136882.1. NC_006396.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1FFKX-ray2.40F14-129[»]
1JJ2X-ray2.40H14-130[»]
1K73X-ray3.01J14-130[»]
1K8AX-ray3.00J14-130[»]
1K9MX-ray3.00J14-130[»]
1KC8X-ray3.01J14-130[»]
1KD1X-ray3.00J14-130[»]
1KQSX-ray3.10H14-130[»]
1M1KX-ray3.20J14-130[»]
1M90X-ray2.80J14-130[»]
1N8RX-ray3.00J14-130[»]
1NJIX-ray3.00J14-130[»]
1Q7YX-ray3.20J14-130[»]
1Q81X-ray2.95J14-130[»]
1Q82X-ray2.98J14-130[»]
1Q86X-ray3.00J14-130[»]
1QVFX-ray3.10H14-130[»]
1QVGX-ray2.90H14-130[»]
1S72X-ray2.40H4-166[»]
1VQ4X-ray2.70H4-166[»]
1VQ5X-ray2.60H4-166[»]
1VQ6X-ray2.70H4-166[»]
1VQ7X-ray2.50H4-166[»]
1VQ8X-ray2.20H4-166[»]
1VQ9X-ray2.40H4-166[»]
1VQKX-ray2.30H4-166[»]
1VQLX-ray2.30H4-166[»]
1VQMX-ray2.30H4-166[»]
1VQNX-ray2.40H4-166[»]
1VQOX-ray2.20H4-166[»]
1VQPX-ray2.25H4-166[»]
1W2BX-ray3.50H14-130[»]
1YHQX-ray2.40H1-177[»]
1YI2X-ray2.65H1-177[»]
1YIJX-ray2.60H1-177[»]
1YITX-ray2.80H1-177[»]
1YJ9X-ray2.90H1-177[»]
1YJNX-ray3.00H1-177[»]
1YJWX-ray2.90H1-177[»]
2OTJX-ray2.90H4-166[»]
2OTLX-ray2.70H4-166[»]
2QA4X-ray3.00H4-166[»]
2QEXX-ray2.90H1-166[»]
3CC2X-ray2.40H1-177[»]
3CC4X-ray2.70H1-177[»]
3CC7X-ray2.70H1-177[»]
3CCEX-ray2.75H1-177[»]
3CCJX-ray2.70H1-177[»]
3CCLX-ray2.90H1-177[»]
3CCMX-ray2.55H1-177[»]
3CCQX-ray2.90H1-177[»]
3CCRX-ray3.00H1-177[»]
3CCSX-ray2.95H1-177[»]
3CCUX-ray2.80H1-177[»]
3CCVX-ray2.90H1-177[»]
3CD6X-ray2.75H1-177[»]
3CMAX-ray2.80H1-177[»]
3CMEX-ray2.95H1-177[»]
3CPWX-ray2.70H1-177[»]
3G4SX-ray3.20H1-177[»]
3G6EX-ray2.70H1-177[»]
3G71X-ray2.85H1-177[»]
3I55X-ray3.11H1-174[»]
3I56X-ray2.90H1-174[»]
4HUBX-ray2.40H1-177[»]
ProteinModelPortalP60617.
SMRP60617. Positions 4-174.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272569.rrnAC2357.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV47175; AAV47175; rrnAC2357.
GeneID3130436.
KEGGhma:rrnAC2357.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0197.
HOGENOMHOG000223755.
KOK02866.
OMAPYTRKEY.
ProtClustDBPRK04199.

Enzyme and pathway databases

BioCycHMAR272569:GJDH-2126-MONOMER.

Family and domain databases

Gene3D3.90.1170.10. 1 hit.
HAMAPMF_00448. Ribosomal_L10e.
InterProIPR001197. Ribosomal_L10e.
IPR016180. Ribosomal_L10e/L16.
IPR022981. Ribosomal_L10e_arc.
IPR018255. Ribosomal_L10e_CS.
[Graphical view]
PfamPF00252. Ribosomal_L16. 1 hit.
[Graphical view]
PIRSFPIRSF005590. Ribosomal_L10. 1 hit.
SUPFAMSSF54686. SSF54686. 1 hit.
PROSITEPS01257. RIBOSOMAL_L10E. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP60617.

Entry information

Entry nameRL10E_HALMA
AccessionPrimary (citable) accession number: P60617
Secondary accession number(s): Q5UZX7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: January 4, 2005
Last modified: February 19, 2014
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references