Reviewed, UniProtKB/Swiss-Prot P60605 (UB2G2_MOUSE)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 G2
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase G2
    Ubiquitin carrier protein G2

Gene names

Name:

Ube2g2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Involved in endoplasmic reticulum-associated degradation (ERAD) By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Ligase
Gene Ontology (GO)
Biological process	ER-associated protein catabolic process Ref.1 Inferred from direct assay. Source: MGI post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Ref.1 Inferred from direct assay. Source: MGI endoplasmic reticulum Ref.1 Inferred from direct assay. Source: MGI
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 165	165	Ubiquitin-conjugating enzyme E2 G2		PRO_0000082484
Sites
	Active site	89	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P60605-1 [UniParc]. Last modified March 15, 2004. Version 1. Checksum: 74DEC732A79575E3	FASTA	165	18,566
	10 20 30 40 50 60 MAGTALKRLM AEYKQLTLNP PEGIVAGPMN EENFFEWEAL IMGPEDTCFE FGVFPAILSF 70 80 90 100 110 120 PLDYPLSPPK MRFTCEMFHP NIYPDGRVCI SILHAPGDDP MGYESSAERW SPVQSVEKIL 130 140 150 160 LSVVSMLAEP NDESGANVDA SKMWRDDREQ FYKIAKQIVQ KSLGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Endoplasmic reticulum (ER)-associated degradation of T cell receptor subunits. Involvement of ER-associated ubiquitin-conjugating enzymes (E2s)." Tiwari S., Weissman A.M. J. Biol. Chem. 276:16193-16200(2001) [PubMed: 11278356] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J. Tissue: Fetus.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF296657 mRNA. Translation: AAK52608.1. BC010321 mRNA. Translation: AAH10321.1.
RefSeq	NP_062777.2.
UniGene	Mm.458125
3D structure databases
HSSP	HSSP built from PDB template 2UCZ based on UniProtKB Q02159.
SMR	P60605. Positions 1-165.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:29061N.
Genome annotation databases
Ensembl	ENSMUSG00000009293. Mus musculus. [Contig view]
GeneID	22213.
KEGG	mmu:22213.
Organism-specific databases
MGI	MGI:1343188. Ube2g2.
Phylogenomic databases
HOVERGEN	P60605.
Gene expression databases
ArrayExpress	P60605.
CleanEx	MM_UBE2G2.
GermOnline	ENSMUSG00000009293. Mus musculus.
Family and domain databases
InterPro	IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
PANTHER	PTHR11621. UBQ-conjugat_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
ProDom	PD000461. UBQ_conjugat. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	302209.
SOURCE	Search...

Entry information

Entry name

UB2G2_MOUSE

Accession

Primary (citable) accession number: P60605
Secondary accession number(s): P56554

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	March 15, 2004
Last modified:	November 25, 2008
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)