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P60605

- UB2G2_MOUSE

UniProt

P60605 - UB2G2_MOUSE

Protein

Ubiquitin-conjugating enzyme E2 G2

Gene

Ube2g2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Involved in endoplasmic reticulum-associated degradation (ERAD) By similarity.By similarity

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei89 – 891Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. identical protein binding Source: IntAct
    4. protein binding Source: UniProtKB
    5. ubiquitin-protein transferase activity Source: UniProtKB

    GO - Biological processi

    1. ER-associated ubiquitin-dependent protein catabolic process Source: MGI
    2. protein K48-linked ubiquitination Source: UniProtKB
    3. protein N-linked glycosylation via asparagine Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 G2 (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein G2
    Ubiquitin-protein ligase G2
    Gene namesi
    Name:Ube2g2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:1343188. Ube2g2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: MGI
    2. endoplasmic reticulum Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 165165Ubiquitin-conjugating enzyme E2 G2PRO_0000082484Add
    BLAST

    Proteomic databases

    PaxDbiP60605.
    PRIDEiP60605.

    PTM databases

    PhosphoSiteiP60605.

    Expressioni

    Gene expression databases

    ArrayExpressiP60605.
    BgeeiP60605.
    CleanExiMM_UBE2G2.
    GenevestigatoriP60605.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself11EBI-9108745,EBI-9108745

    Protein-protein interaction databases

    BioGridi204416. 6 interactions.
    DIPiDIP-29061N.

    Structurei

    Secondary structure

    165
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi1 – 1818
    Beta strandi24 – 307
    Beta strandi36 – 427
    Turni48 – 514
    Beta strandi53 – 597
    Turni62 – 654
    Beta strandi70 – 756
    Beta strandi86 – 883
    Helixi91 – 933
    Helixi116 – 12813
    Helixi132 – 1343
    Helixi138 – 1458
    Helixi148 – 16215

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3FSHX-ray2.76A/B1-165[»]
    ProteinModelPortaliP60605.
    SMRiP60605. Positions 2-165.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP60605.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    HOVERGENiHBG063308.
    InParanoidiP60605.
    KOiK04555.
    OMAiDMFHPNI.
    OrthoDBiEOG7VB2HT.
    TreeFamiTF101118.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P60605-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGTALKRLM AEYKQLTLNP PEGIVAGPMN EENFFEWEAL IMGPEDTCFE    50
    FGVFPAILSF PLDYPLSPPK MRFTCEMFHP NIYPDGRVCI SILHAPGDDP 100
    MGYESSAERW SPVQSVEKIL LSVVSMLAEP NDESGANVDA SKMWRDDREQ 150
    FYKIAKQIVQ KSLGL 165
    Length:165
    Mass (Da):18,566
    Last modified:March 15, 2004 - v1
    Checksum:i74DEC732A79575E3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF296657 mRNA. Translation: AAK52608.1.
    BC010321 mRNA. Translation: AAH10321.1.
    CCDSiCCDS56722.1.
    RefSeqiNP_062777.2. NM_019803.3.
    UniGeneiMm.458125.

    Genome annotation databases

    EnsembliENSMUST00000174510; ENSMUSP00000133515; ENSMUSG00000009293.
    GeneIDi22213.
    KEGGimmu:22213.
    UCSCiuc007fwa.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF296657 mRNA. Translation: AAK52608.1 .
    BC010321 mRNA. Translation: AAH10321.1 .
    CCDSi CCDS56722.1.
    RefSeqi NP_062777.2. NM_019803.3.
    UniGenei Mm.458125.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3FSH X-ray 2.76 A/B 1-165 [» ]
    ProteinModelPortali P60605.
    SMRi P60605. Positions 2-165.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204416. 6 interactions.
    DIPi DIP-29061N.

    PTM databases

    PhosphoSitei P60605.

    Proteomic databases

    PaxDbi P60605.
    PRIDEi P60605.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000174510 ; ENSMUSP00000133515 ; ENSMUSG00000009293 .
    GeneIDi 22213.
    KEGGi mmu:22213.
    UCSCi uc007fwa.1. mouse.

    Organism-specific databases

    CTDi 7327.
    MGIi MGI:1343188. Ube2g2.

    Phylogenomic databases

    eggNOGi COG5078.
    HOVERGENi HBG063308.
    InParanoidi P60605.
    KOi K04555.
    OMAi DMFHPNI.
    OrthoDBi EOG7VB2HT.
    TreeFami TF101118.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    ChiTaRSi UBE2G2. mouse.
    EvolutionaryTracei P60605.
    NextBioi 28670.
    PROi P60605.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P60605.
    Bgeei P60605.
    CleanExi MM_UBE2G2.
    Genevestigatori P60605.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Endoplasmic reticulum (ER)-associated degradation of T cell receptor subunits. Involvement of ER-associated ubiquitin-conjugating enzymes (E2s)."
      Tiwari S., Weissman A.M.
      J. Biol. Chem. 276:16193-16200(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6J.
      Tissue: Fetus.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiUB2G2_MOUSE
    AccessioniPrimary (citable) accession number: P60605
    Secondary accession number(s): P56554
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2004
    Last sequence update: March 15, 2004
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3