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Protein

Ubiquitin-conjugating enzyme E2 G2

Gene

Ube2g2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Involved in endoplasmic reticulum-associated degradation (ERAD).By similarity

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotationBy similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei89Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 G2 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme G2
Ubiquitin carrier protein G2
Ubiquitin-protein ligase G2
Gene namesi
Name:Ube2g2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1343188. Ube2g2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: MGI
  • endoplasmic reticulum Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000824842 – 165Ubiquitin-conjugating enzyme E2 G2Add BLAST164

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP60605.
PaxDbiP60605.
PeptideAtlasiP60605.
PRIDEiP60605.

PTM databases

iPTMnetiP60605.
PhosphoSitePlusiP60605.

Expressioni

Gene expression databases

BgeeiENSMUSG00000009293.
CleanExiMM_UBE2G2.
ExpressionAtlasiP60605. baseline and differential.
GenevisibleiP60605. MM.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself11EBI-9108745,EBI-9108745

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • ubiquitin protein ligase binding Source: GO_Central

Protein-protein interaction databases

BioGridi204416. 8 interactors.
DIPiDIP-29061N.
STRINGi10090.ENSMUSP00000133515.

Structurei

Secondary structure

1165
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 18Combined sources17
Beta strandi24 – 30Combined sources7
Beta strandi36 – 42Combined sources7
Turni48 – 51Combined sources4
Beta strandi53 – 59Combined sources7
Turni62 – 65Combined sources4
Beta strandi70 – 75Combined sources6
Beta strandi86 – 88Combined sources3
Helixi91 – 93Combined sources3
Helixi116 – 128Combined sources13
Helixi132 – 134Combined sources3
Helixi138 – 145Combined sources8
Helixi148 – 162Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FSHX-ray2.76A/B1-165[»]
ProteinModelPortaliP60605.
SMRiP60605.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60605.

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0426. Eukaryota.
ENOG410Y0NU. LUCA.
GeneTreeiENSGT00730000110436.
HOVERGENiHBG063308.
InParanoidiP60605.
KOiK04555.
OMAiGVFPAKL.
OrthoDBiEOG091G0O9F.
TreeFamiTF101118.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60605-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGTALKRLM AEYKQLTLNP PEGIVAGPMN EENFFEWEAL IMGPEDTCFE
60 70 80 90 100
FGVFPAILSF PLDYPLSPPK MRFTCEMFHP NIYPDGRVCI SILHAPGDDP
110 120 130 140 150
MGYESSAERW SPVQSVEKIL LSVVSMLAEP NDESGANVDA SKMWRDDREQ
160
FYKIAKQIVQ KSLGL
Length:165
Mass (Da):18,566
Last modified:March 15, 2004 - v1
Checksum:i74DEC732A79575E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296657 mRNA. Translation: AAK52608.1.
BC010321 mRNA. Translation: AAH10321.1.
CCDSiCCDS56722.1.
RefSeqiNP_062777.2. NM_019803.3.
UniGeneiMm.458125.

Genome annotation databases

EnsembliENSMUST00000174510; ENSMUSP00000133515; ENSMUSG00000009293.
GeneIDi22213.
KEGGimmu:22213.
UCSCiuc007fwa.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296657 mRNA. Translation: AAK52608.1.
BC010321 mRNA. Translation: AAH10321.1.
CCDSiCCDS56722.1.
RefSeqiNP_062777.2. NM_019803.3.
UniGeneiMm.458125.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FSHX-ray2.76A/B1-165[»]
ProteinModelPortaliP60605.
SMRiP60605.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204416. 8 interactors.
DIPiDIP-29061N.
STRINGi10090.ENSMUSP00000133515.

PTM databases

iPTMnetiP60605.
PhosphoSitePlusiP60605.

Proteomic databases

EPDiP60605.
PaxDbiP60605.
PeptideAtlasiP60605.
PRIDEiP60605.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000174510; ENSMUSP00000133515; ENSMUSG00000009293.
GeneIDi22213.
KEGGimmu:22213.
UCSCiuc007fwa.1. mouse.

Organism-specific databases

CTDi7327.
MGIiMGI:1343188. Ube2g2.

Phylogenomic databases

eggNOGiKOG0426. Eukaryota.
ENOG410Y0NU. LUCA.
GeneTreeiENSGT00730000110436.
HOVERGENiHBG063308.
InParanoidiP60605.
KOiK04555.
OMAiGVFPAKL.
OrthoDBiEOG091G0O9F.
TreeFamiTF101118.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.

Miscellaneous databases

EvolutionaryTraceiP60605.
PROiP60605.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000009293.
CleanExiMM_UBE2G2.
ExpressionAtlasiP60605. baseline and differential.
GenevisibleiP60605. MM.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUB2G2_MOUSE
AccessioniPrimary (citable) accession number: P60605
Secondary accession number(s): P56554
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: March 15, 2004
Last modified: November 30, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.