P60605 (UB2G2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 G2
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein G2
Ubiquitin-protein ligase G2

Gene names

Name:

Ube2g2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Involved in endoplasmic reticulum-associated degradation (ERAD) By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER-associated protein catabolic process Inferred from direct assay Ref.1. Source: MGI protein K48-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein N-linked glycosylation via asparagine Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytosol Inferred from direct assay Ref.1. Source: MGI endoplasmic reticulum Inferred from direct assay Ref.1. Source: MGI
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 165

165

Ubiquitin-conjugating enzyme E2 G2

PRO_0000082484

Sites

Active site

89

1

Glycyl thioester intermediate By similarity

Secondary structure

1

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165

Helix Strand Turn

Details...

Helix

1 – 18

18

Beta strand

24 – 30

7

Beta strand

36 – 42

7

Turn

48 – 51

4

Beta strand

53 – 59

7

Turn

62 – 65

4

Beta strand

70 – 75

6

Beta strand

86 – 88

3

Helix

91 – 93

3

Helix

116 – 128

13

Helix

132 – 134

3

Helix

138 – 145

8

Helix

148 – 162

15

Sequences

	Sequence		Length	Mass (Da)
	P60605 [UniParc]. Last modified March 15, 2004. Version 1. Checksum: 74DEC732A79575E3	FASTA	165	18,566
	10 20 30 40 50 60 MAGTALKRLM AEYKQLTLNP PEGIVAGPMN EENFFEWEAL IMGPEDTCFE FGVFPAILSF 70 80 90 100 110 120 PLDYPLSPPK MRFTCEMFHP NIYPDGRVCI SILHAPGDDP MGYESSAERW SPVQSVEKIL 130 140 150 160 LSVVSMLAEP NDESGANVDA SKMWRDDREQ FYKIAKQIVQ KSLGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Endoplasmic reticulum (ER)-associated degradation of T cell receptor subunits. Involvement of ER-associated ubiquitin-conjugating enzymes (E2s)." Tiwari S., Weissman A.M. J. Biol. Chem. 276:16193-16200(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J. Tissue: Fetus.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF296657 mRNA. Translation: AAK52608.1.
BC010321 mRNA. Translation: AAH10321.1.

IPI

IPI01026711.

RefSeq

NP_062777.2. NM_019803.3.

UniGene

Mm.458125.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3FSH	X-ray	2.76	A/B	1-165	[»]

ProteinModelPortal

P60605.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-29061N.

PTM databases

PhosphoSite

P60605.

Proteomic databases

PaxDb

P60605.

PRIDE

P60605.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000174510; ENSMUSP00000133515; ENSMUSG00000009293.

GeneID

22213.

KEGG

mmu:22213.

Organism-specific databases

CTD

7327.

MGI

MGI:1343188. Ube2g2.

Phylogenomic databases

eggNOG

COG5078.

HOVERGEN

HBG063308.

InParanoid

P60605.

KO

K04555.

OMA

RRLMAEY.

OrthoDB

EOG4GB77C.

Enzyme and pathway databases

UniPathway

UPA00143.

Gene expression databases

ArrayExpress

P60605.

Bgee

P60605.

CleanEx

MM_UBE2G2.

Genevestigator

P60605.

GermOnline

ENSMUSG00000009293. Mus musculus.

Family and domain databases

Gene3D

3.10.110.10. 1 hit.

InterPro

IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]

Pfam

PF00179. UQ_con. 1 hit.
[Graphical view]

SUPFAM

SSF54495. UBQ-conjugat/RWD-like. 1 hit.

PROSITE

PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

UBE2G2. mouse.

EvolutionaryTrace

P60605.

NextBio

28670.

SOURCE

Search...

Entry information

Entry name

UB2G2_MOUSE

Accession

Primary (citable) accession number: P60605
Secondary accession number(s): P56554

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	March 15, 2004
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program