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Protein

Ubiquitin-conjugating enzyme E2 G2

Gene

UBE2G2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Involved in endoplasmic reticulum-associated degradation (ERAD).2 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei89Glycyl thioester intermediate1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin conjugating enzyme activity Source: MGI
  • ubiquitin protein ligase activity Source: GO_Central
  • ubiquitin protein ligase binding Source: GO_Central
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  • cellular protein catabolic process Source: MGI
  • cellular response to interferon-beta Source: UniProtKB
  • ER-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
  • negative regulation of retrograde protein transport, ER to cytosol Source: ParkinsonsUK-UCL
  • protein K48-linked ubiquitination Source: UniProtKB
  • protein N-linked glycosylation via asparagine Source: UniProtKB
  • ubiquitin-dependent protein catabolic process Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS00052-MONOMER.
BRENDAi2.3.2.B6. 2681.
ReactomeiR-HSA-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
R-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiP60604.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 G2 (EC:2.3.2.231 Publication)
Alternative name(s):
E2 ubiquitin-conjugating enzyme G2
Ubiquitin carrier protein G2
Ubiquitin-protein ligase G2
Gene namesi
Name:UBE2G2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 21

Organism-specific databases

HGNCiHGNC:12483. UBE2G2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • endoplasmic reticulum Source: Ensembl
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi7327.
OpenTargetsiENSG00000184787.
PharmGKBiPA37132.

Polymorphism and mutation databases

BioMutaiUBE2G2.
DMDMi45593583.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000824832 – 165Ubiquitin-conjugating enzyme E2 G2Add BLAST164

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP60604.
MaxQBiP60604.
PaxDbiP60604.
PeptideAtlasiP60604.
PRIDEiP60604.
TopDownProteomicsiP60604-1. [P60604-1]

PTM databases

iPTMnetiP60604.
PhosphoSitePlusiP60604.
SwissPalmiP60604.

Expressioni

Gene expression databases

BgeeiENSG00000184787.
CleanExiHS_UBE2G2.
ExpressionAtlasiP60604. baseline and differential.
GenevisibleiP60604. HS.

Organism-specific databases

HPAiHPA003332.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
AMFRQ9UKV518EBI-1051028,EBI-1046367

GO - Molecular functioni

Protein-protein interaction databases

BioGridi113175. 52 interactors.
DIPiDIP-50750N.
IntActiP60604. 28 interactors.
STRINGi9606.ENSP00000338348.

Structurei

Secondary structure

1165
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 18Combined sources15
Beta strandi24 – 30Combined sources7
Beta strandi36 – 42Combined sources7
Turni48 – 51Combined sources4
Beta strandi53 – 59Combined sources7
Turni62 – 65Combined sources4
Beta strandi70 – 73Combined sources4
Beta strandi86 – 88Combined sources3
Helixi91 – 93Combined sources3
Helixi105 – 108Combined sources4
Helixi116 – 128Combined sources13
Helixi135 – 137Combined sources3
Helixi138 – 146Combined sources9
Helixi148 – 163Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CYXX-ray2.56A/B/C1-165[»]
2KLYNMR-A1-165[»]
2LXPNMR-A2-165[»]
3H8KX-ray1.80A2-165[»]
4LADX-ray2.30A1-165[»]
ProteinModelPortaliP60604.
SMRiP60604.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60604.

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0426. Eukaryota.
ENOG410Y0NU. LUCA.
GeneTreeiENSGT00730000110436.
HOGENOMiHOG000233454.
HOVERGENiHBG063308.
InParanoidiP60604.
KOiK04555.
OMAiGVFPAKL.
OrthoDBiEOG091G0O9F.
PhylomeDBiP60604.
TreeFamiTF101118.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P60604-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGTALKRLM AEYKQLTLNP PEGIVAGPMN EENFFEWEAL IMGPEDTCFE
60 70 80 90 100
FGVFPAILSF PLDYPLSPPK MRFTCEMFHP NIYPDGRVCI SILHAPGDDP
110 120 130 140 150
MGYESSAERW SPVQSVEKIL LSVVSMLAEP NDESGANVDA SKMWRDDREQ
160
FYKIAKQIVQ KSLGL
Length:165
Mass (Da):18,566
Last modified:March 15, 2004 - v1
Checksum:i74DEC732A79575E3
GO
Isoform 2 (identifier: P60604-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: Missing.

Note: No experimental confirmation available. Gene prediction based on EST data.
Show »
Length:137
Mass (Da):15,614
Checksum:iFA95FDC41C3FCAD4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12E → V in AAC32312 (PubMed:9693041).Curated1
Sequence conflicti101 – 107MGYESSA → HGLREQP in AAC32312 (PubMed:9693041).Curated7

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0462601 – 28Missing in isoform 2. CuratedAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF032456 mRNA. Translation: AAC32312.1.
BT006914 mRNA. Translation: AAP35560.1.
AK290629 mRNA. Translation: BAF83318.1.
AL163300 Genomic DNA. Translation: CAB90551.1.
CH471079 Genomic DNA. Translation: EAX09395.1.
CH471079 Genomic DNA. Translation: EAX09397.1.
CH471079 Genomic DNA. Translation: EAX09398.1.
CH471079 Genomic DNA. Translation: EAX09399.1.
BC001738 mRNA. Translation: AAH01738.1.
BC008351 mRNA. Translation: AAH08351.1.
BC011569 mRNA. Translation: AAH11569.1.
CCDSiCCDS13714.1. [P60604-1]
CCDS33586.1. [P60604-2]
RefSeqiNP_001189418.1. NM_001202489.1.
NP_003334.2. NM_003343.5. [P60604-1]
NP_872630.1. NM_182688.2. [P60604-2]
XP_016883958.1. XM_017028469.1. [P60604-2]
UniGeneiHs.529420.
Hs.701398.

Genome annotation databases

EnsembliENST00000330942; ENSP00000331384; ENSG00000184787. [P60604-2]
ENST00000345496; ENSP00000338348; ENSG00000184787. [P60604-1]
GeneIDi7327.
KEGGihsa:7327.
UCSCiuc002zfx.4. human. [P60604-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF032456 mRNA. Translation: AAC32312.1.
BT006914 mRNA. Translation: AAP35560.1.
AK290629 mRNA. Translation: BAF83318.1.
AL163300 Genomic DNA. Translation: CAB90551.1.
CH471079 Genomic DNA. Translation: EAX09395.1.
CH471079 Genomic DNA. Translation: EAX09397.1.
CH471079 Genomic DNA. Translation: EAX09398.1.
CH471079 Genomic DNA. Translation: EAX09399.1.
BC001738 mRNA. Translation: AAH01738.1.
BC008351 mRNA. Translation: AAH08351.1.
BC011569 mRNA. Translation: AAH11569.1.
CCDSiCCDS13714.1. [P60604-1]
CCDS33586.1. [P60604-2]
RefSeqiNP_001189418.1. NM_001202489.1.
NP_003334.2. NM_003343.5. [P60604-1]
NP_872630.1. NM_182688.2. [P60604-2]
XP_016883958.1. XM_017028469.1. [P60604-2]
UniGeneiHs.529420.
Hs.701398.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CYXX-ray2.56A/B/C1-165[»]
2KLYNMR-A1-165[»]
2LXPNMR-A2-165[»]
3H8KX-ray1.80A2-165[»]
4LADX-ray2.30A1-165[»]
ProteinModelPortaliP60604.
SMRiP60604.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113175. 52 interactors.
DIPiDIP-50750N.
IntActiP60604. 28 interactors.
STRINGi9606.ENSP00000338348.

PTM databases

iPTMnetiP60604.
PhosphoSitePlusiP60604.
SwissPalmiP60604.

Polymorphism and mutation databases

BioMutaiUBE2G2.
DMDMi45593583.

Proteomic databases

EPDiP60604.
MaxQBiP60604.
PaxDbiP60604.
PeptideAtlasiP60604.
PRIDEiP60604.
TopDownProteomicsiP60604-1. [P60604-1]

Protocols and materials databases

DNASUi7327.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000330942; ENSP00000331384; ENSG00000184787. [P60604-2]
ENST00000345496; ENSP00000338348; ENSG00000184787. [P60604-1]
GeneIDi7327.
KEGGihsa:7327.
UCSCiuc002zfx.4. human. [P60604-1]

Organism-specific databases

CTDi7327.
DisGeNETi7327.
GeneCardsiUBE2G2.
HGNCiHGNC:12483. UBE2G2.
HPAiHPA003332.
MIMi603124. gene.
neXtProtiNX_P60604.
OpenTargetsiENSG00000184787.
PharmGKBiPA37132.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0426. Eukaryota.
ENOG410Y0NU. LUCA.
GeneTreeiENSGT00730000110436.
HOGENOMiHOG000233454.
HOVERGENiHBG063308.
InParanoidiP60604.
KOiK04555.
OMAiGVFPAKL.
OrthoDBiEOG091G0O9F.
PhylomeDBiP60604.
TreeFamiTF101118.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciZFISH:HS00052-MONOMER.
BRENDAi2.3.2.B6. 2681.
ReactomeiR-HSA-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
R-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiP60604.

Miscellaneous databases

ChiTaRSiUBE2G2. human.
EvolutionaryTraceiP60604.
GeneWikiiUBE2G2.
GenomeRNAii7327.
PROiP60604.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000184787.
CleanExiHS_UBE2G2.
ExpressionAtlasiP60604. baseline and differential.
GenevisibleiP60604. HS.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUB2G2_HUMAN
AccessioniPrimary (citable) accession number: P60604
Secondary accession number(s): A6NMQ7
, A8K3L4, D3DSL7, P56554
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: March 15, 2004
Last modified: November 30, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 21
    Human chromosome 21: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.