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P60604

- UB2G2_HUMAN

UniProt

P60604 - UB2G2_HUMAN

Protein

Ubiquitin-conjugating enzyme E2 G2

Gene

UBE2G2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (15 Mar 2004)
      Previous versions | rss
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    Functioni

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Involved in endoplasmic reticulum-associated degradation (ERAD).2 Publications

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei89 – 891Glycyl thioester intermediate

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB
    4. ubiquitin-protein transferase activity Source: UniProtKB

    GO - Biological processi

    1. ER-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
    2. protein K48-linked ubiquitination Source: UniProtKB
    3. protein N-linked glycosylation via asparagine Source: UniProtKB
    4. ubiquitin-dependent protein catabolic process Source: ProtInc

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
    SignaLinkiP60604.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 G2 (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein G2
    Ubiquitin-protein ligase G2
    Gene namesi
    Name:UBE2G2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 21

    Organism-specific databases

    HGNCiHGNC:12483. UBE2G2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. endoplasmic reticulum Source: Ensembl

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA37132.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 165165Ubiquitin-conjugating enzyme E2 G2PRO_0000082483Add
    BLAST

    Proteomic databases

    MaxQBiP60604.
    PaxDbiP60604.
    PRIDEiP60604.

    PTM databases

    PhosphoSiteiP60604.

    Expressioni

    Gene expression databases

    ArrayExpressiP60604.
    BgeeiP60604.
    CleanExiHS_UBE2G2.
    GenevestigatoriP60604.

    Organism-specific databases

    HPAiHPA003332.

    Interactioni

    Protein-protein interaction databases

    BioGridi113175. 43 interactions.
    IntActiP60604. 23 interactions.
    STRINGi9606.ENSP00000338348.

    Structurei

    Secondary structure

    1
    165
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 1815
    Beta strandi24 – 307
    Beta strandi36 – 427
    Turni48 – 514
    Beta strandi53 – 597
    Turni62 – 654
    Beta strandi70 – 734
    Beta strandi86 – 883
    Helixi91 – 933
    Helixi105 – 1084
    Helixi116 – 12813
    Helixi135 – 1373
    Helixi138 – 1469
    Helixi148 – 16316

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CYXX-ray2.56A/B/C1-165[»]
    2KLYNMR-A1-165[»]
    2LXPNMR-A2-165[»]
    3H8KX-ray1.80A2-165[»]
    4LADX-ray2.30A1-165[»]
    ProteinModelPortaliP60604.
    SMRiP60604. Positions 2-165.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP60604.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    HOGENOMiHOG000233454.
    HOVERGENiHBG063308.
    KOiK04555.
    OMAiDMFHPNI.
    OrthoDBiEOG7VB2HT.
    PhylomeDBiP60604.
    TreeFamiTF101118.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P60604-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAGTALKRLM AEYKQLTLNP PEGIVAGPMN EENFFEWEAL IMGPEDTCFE    50
    FGVFPAILSF PLDYPLSPPK MRFTCEMFHP NIYPDGRVCI SILHAPGDDP 100
    MGYESSAERW SPVQSVEKIL LSVVSMLAEP NDESGANVDA SKMWRDDREQ 150
    FYKIAKQIVQ KSLGL 165
    Length:165
    Mass (Da):18,566
    Last modified:March 15, 2004 - v1
    Checksum:i74DEC732A79575E3
    GO
    Isoform 2 (identifier: P60604-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-28: Missing.

    Note: No experimental confirmation available. Gene prediction based on EST data.

    Show »
    Length:137
    Mass (Da):15,614
    Checksum:iFA95FDC41C3FCAD4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti12 – 121E → V in AAC32312. (PubMed:9693041)Curated
    Sequence conflicti101 – 1077MGYESSA → HGLREQP in AAC32312. (PubMed:9693041)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2828Missing in isoform 2. CuratedVSP_046260Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF032456 mRNA. Translation: AAC32312.1.
    BT006914 mRNA. Translation: AAP35560.1.
    AK290629 mRNA. Translation: BAF83318.1.
    AL163300 Genomic DNA. Translation: CAB90551.1.
    CH471079 Genomic DNA. Translation: EAX09395.1.
    CH471079 Genomic DNA. Translation: EAX09397.1.
    CH471079 Genomic DNA. Translation: EAX09398.1.
    CH471079 Genomic DNA. Translation: EAX09399.1.
    BC001738 mRNA. Translation: AAH01738.1.
    BC008351 mRNA. Translation: AAH08351.1.
    BC011569 mRNA. Translation: AAH11569.1.
    CCDSiCCDS13714.1. [P60604-1]
    CCDS33586.1. [P60604-2]
    RefSeqiNP_001189418.1. NM_001202489.1.
    NP_003334.2. NM_003343.5. [P60604-1]
    NP_872630.1. NM_182688.2. [P60604-2]
    UniGeneiHs.529420.
    Hs.701398.

    Genome annotation databases

    EnsembliENST00000330942; ENSP00000331384; ENSG00000184787. [P60604-2]
    ENST00000345496; ENSP00000338348; ENSG00000184787. [P60604-1]
    GeneIDi7327.
    KEGGihsa:7327.
    UCSCiuc002zfx.3. human. [P60604-1]

    Polymorphism databases

    DMDMi45593583.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF032456 mRNA. Translation: AAC32312.1 .
    BT006914 mRNA. Translation: AAP35560.1 .
    AK290629 mRNA. Translation: BAF83318.1 .
    AL163300 Genomic DNA. Translation: CAB90551.1 .
    CH471079 Genomic DNA. Translation: EAX09395.1 .
    CH471079 Genomic DNA. Translation: EAX09397.1 .
    CH471079 Genomic DNA. Translation: EAX09398.1 .
    CH471079 Genomic DNA. Translation: EAX09399.1 .
    BC001738 mRNA. Translation: AAH01738.1 .
    BC008351 mRNA. Translation: AAH08351.1 .
    BC011569 mRNA. Translation: AAH11569.1 .
    CCDSi CCDS13714.1. [P60604-1 ]
    CCDS33586.1. [P60604-2 ]
    RefSeqi NP_001189418.1. NM_001202489.1.
    NP_003334.2. NM_003343.5. [P60604-1 ]
    NP_872630.1. NM_182688.2. [P60604-2 ]
    UniGenei Hs.529420.
    Hs.701398.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2CYX X-ray 2.56 A/B/C 1-165 [» ]
    2KLY NMR - A 1-165 [» ]
    2LXP NMR - A 2-165 [» ]
    3H8K X-ray 1.80 A 2-165 [» ]
    4LAD X-ray 2.30 A 1-165 [» ]
    ProteinModelPortali P60604.
    SMRi P60604. Positions 2-165.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 113175. 43 interactions.
    IntActi P60604. 23 interactions.
    STRINGi 9606.ENSP00000338348.

    PTM databases

    PhosphoSitei P60604.

    Polymorphism databases

    DMDMi 45593583.

    Proteomic databases

    MaxQBi P60604.
    PaxDbi P60604.
    PRIDEi P60604.

    Protocols and materials databases

    DNASUi 7327.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000330942 ; ENSP00000331384 ; ENSG00000184787 . [P60604-2 ]
    ENST00000345496 ; ENSP00000338348 ; ENSG00000184787 . [P60604-1 ]
    GeneIDi 7327.
    KEGGi hsa:7327.
    UCSCi uc002zfx.3. human. [P60604-1 ]

    Organism-specific databases

    CTDi 7327.
    GeneCardsi GC21M046188.
    HGNCi HGNC:12483. UBE2G2.
    HPAi HPA003332.
    MIMi 603124. gene.
    neXtProti NX_P60604.
    PharmGKBi PA37132.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5078.
    HOGENOMi HOG000233454.
    HOVERGENi HBG063308.
    KOi K04555.
    OMAi DMFHPNI.
    OrthoDBi EOG7VB2HT.
    PhylomeDBi P60604.
    TreeFami TF101118.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
    SignaLinki P60604.

    Miscellaneous databases

    ChiTaRSi UBE2G2. human.
    EvolutionaryTracei P60604.
    GeneWikii UBE2G2.
    GenomeRNAii 7327.
    NextBioi 28670.
    PROi P60604.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P60604.
    Bgeei P60604.
    CleanExi HS_UBE2G2.
    Genevestigatori P60604.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification, expression, and chromosomal localization of ubiquitin conjugating enzyme 7 (UBE2G2), a human homologue of the Saccharomyces cerevisiae UBC7 gene."
      Katsanis N., Fisher E.M.C.
      Genomics 51:128-131(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Heart.
    4. "The DNA sequence of human chromosome 21."
      Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A.
      , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.
      Nature 405:311-319(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Lung.
    7. "The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines."
      David Y., Ziv T., Admon A., Navon A.
      J. Biol. Chem. 285:8595-8604(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "STT3B-dependent posttranslational N-glycosylation as a surveillance system for secretory protein."
      Sato T., Sako Y., Sho M., Momohara M., Suico M.A., Shuto T., Nishitoh H., Okiyoneda T., Kokame K., Kaneko M., Taura M., Miyata M., Chosa K., Koga T., Morino-Koga S., Wada I., Kai H.
      Mol. Cell 47:99-110(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN ERAD PATHWAY.
    10. "Structure of human ubiquitin-conjugating enzyme E2 G2 (UBE2G2/UBC7)."
      Arai R., Yoshikawa S., Murayama K., Imai Y., Takahashi R., Shirouzu M., Yokoyama S.
      Acta Crystallogr. F 62:330-334(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.56 ANGSTROMS).

    Entry informationi

    Entry nameiUB2G2_HUMAN
    AccessioniPrimary (citable) accession number: P60604
    Secondary accession number(s): A6NMQ7
    , A8K3L4, D3DSL7, P56554
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2004
    Last sequence update: March 15, 2004
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 21
      Human chromosome 21: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3