Reviewed, UniProtKB/Swiss-Prot P60604 (UB2G2_HUMAN)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 G2
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase G2
    Ubiquitin carrier protein G2

Gene names

Name:

UBE2G2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Involved in endoplasmic reticulum-associated degradation (ERAD).
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro ubiquitin-dependent protein catabolic process Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cytosol Inferred from direct assay. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: UniProtKB ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ALG11	Q2TAA5	1	EBI-1051028,EBI-1048799
AMFR	Q9UKV5	1	EBI-1051028,EBI-1046367
AUP1	Q9Y679	1	EBI-1051028,EBI-1058701
CORO1A	P31146	1	EBI-1051028,EBI-1046676
MALT1	Q9UDY8	1	EBI-1051028,EBI-1047372
USP15	Q9Y4E8	1	EBI-1051028,EBI-1043104

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 165

165

Ubiquitin-conjugating enzyme E2 G2

PRO_0000082483

Sites

Active site

89

1

Glycyl thioester intermediate

Experimental info

Sequence conflict

12

1

E → V in AAC32312. Ref.1

Sequence conflict

101 – 107

7

MGYESSA → HGLREQP in AAC32312. Ref.1

Secondary structure

1

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165

Helix Strand Turn

Details...

Helix

1 – 18

18

Beta strand

24 – 30

7

Beta strand

36 – 42

7

Turn

48 – 51

4

Beta strand

53 – 59

7

Turn

62 – 65

4

Beta strand

70 – 75

6

Beta strand

86 – 88

3

Helix

91 – 93

3

Helix

105 – 108

4

Helix

116 – 128

13

Helix

135 – 137

3

Helix

138 – 145

8

Helix

148 – 162

15

Sequences

	Sequence		Length	Mass (Da)
	P60604-1 [UniParc]. Last modified March 15, 2004. Version 1. Checksum: 74DEC732A79575E3	FASTA	165	18,566
	10 20 30 40 50 60 MAGTALKRLM AEYKQLTLNP PEGIVAGPMN EENFFEWEAL IMGPEDTCFE FGVFPAILSF 70 80 90 100 110 120 PLDYPLSPPK MRFTCEMFHP NIYPDGRVCI SILHAPGDDP MGYESSAERW SPVQSVEKIL 130 140 150 160 LSVVSMLAEP NDESGANVDA SKMWRDDREQ FYKIAKQIVQ KSLGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification, expression, and chromosomal localization of ubiquitin conjugating enzyme 7 (UBE2G2), a human homologue of the Saccharomyces cerevisiae UBC7 gene." Katsanis N., Fisher E.M.C. Genomics 51:128-131(1998) [PubMed: 9693041] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The DNA sequence of human chromosome 21." Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A. , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L. Nature 405:311-319(2000) [PubMed: 10830953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[5]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[6]	"Structure of human ubiquitin-conjugating enzyme E2 G2 (UBE2G2/UBC7)." Arai R., Yoshikawa S., Murayama K., Imai Y., Takahashi R., Shirouzu M., Yokoyama S. Acta Crystallogr. F 62:330-334(2006) [PubMed: 16582478] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.56 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF032456 mRNA. Translation: AAC32312.1.
BT006914 mRNA. Translation: AAP35560.1.
AL163300 Genomic DNA. Translation: CAB90551.1.
BC001738 mRNA. Translation: AAH01738.1.
BC008351 mRNA. Translation: AAH08351.1.
BC011569 mRNA. Translation: AAH11569.1.

IPI

IPI00010142.

RefSeq

NP_003334.2.
NP_872630.1.

UniGene

Hs.529420
Hs.701398

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CYX	X-ray	2.56	A/B/C	1-165	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P60604. 6 interactions.

Proteomic databases

PRIDE

P60604.

Genome annotation databases

Ensembl

ENSG00000184787. Homo sapiens. [Contig view]

GeneID

7327.

KEGG

hsa:7327.

Organism-specific databases

GeneCards

GC21M045013.

H-InvDB

HIX0016172.

HGNC

HGNC:12483. UBE2G2.

HPA

HPA003332.

MIM

603124. gene.

PharmGKB

PA37132.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P60604.

HOVERGEN

P60604.

Enzyme and pathway databases

BRENDA

6.3.2.19. 247.

Gene expression databases

ArrayExpress

P60604.

Bgee

P60604.

CleanEx

HS_UBE2G2.

GermOnline

ENSG00000184787. Homo sapiens.

Family and domain databases

InterPro

IPR016135. UBQ-conjugat/RWD-like.
IPR000608. UBQ-conjugat_E2.
[Graphical view]

Gene3D

G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.

Pfam

PF00179. UQ_con. 1 hit.
[Graphical view]

ProDom

PD000461. UBQ_conjugat. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00212. UBCc. 1 hit.
[Graphical view]

PROSITE

PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

28670.

SOURCE

Search...

Entry information

Entry name

UB2G2_HUMAN

Accession

Primary (citable) accession number: P60604
Secondary accession number(s): P56554

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	March 15, 2004
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)