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Protein

Ubiquitin-conjugating enzyme E2 G2

Gene

UBE2G2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Involved in endoplasmic reticulum-associated degradation (ERAD).2 Publications

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891Glycyl thioester intermediate

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ligase activity Source: UniProtKB-KW
  3. ubiquitin conjugating enzyme activity Source: MGI
  4. ubiquitin-like protein transferase activity Source: GO_Central
  5. ubiquitin protein ligase activity Source: GO_Central
  6. ubiquitin protein ligase binding Source: GO_Central
  7. ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  1. cellular protein catabolic process Source: MGI
  2. ER-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
  3. protein K48-linked ubiquitination Source: UniProtKB
  4. protein N-linked glycosylation via asparagine Source: UniProtKB
  5. ubiquitin-dependent protein catabolic process Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.3.2.B6. 2681.
ReactomeiREACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiP60604.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 G2 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein G2
Ubiquitin-protein ligase G2
Gene namesi
Name:UBE2G2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 21

Organism-specific databases

HGNCiHGNC:12483. UBE2G2.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. endoplasmic reticulum Source: GO_Central
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37132.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 165165Ubiquitin-conjugating enzyme E2 G2PRO_0000082483Add
BLAST

Proteomic databases

MaxQBiP60604.
PaxDbiP60604.
PRIDEiP60604.

PTM databases

PhosphoSiteiP60604.

Expressioni

Gene expression databases

BgeeiP60604.
CleanExiHS_UBE2G2.
ExpressionAtlasiP60604. baseline and differential.
GenevestigatoriP60604.

Organism-specific databases

HPAiHPA003332.

Interactioni

Protein-protein interaction databases

BioGridi113175. 44 interactions.
DIPiDIP-50750N.
IntActiP60604. 24 interactions.
STRINGi9606.ENSP00000338348.

Structurei

Secondary structure

1
165
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1815Combined sources
Beta strandi24 – 307Combined sources
Beta strandi36 – 427Combined sources
Turni48 – 514Combined sources
Beta strandi53 – 597Combined sources
Turni62 – 654Combined sources
Beta strandi70 – 734Combined sources
Beta strandi86 – 883Combined sources
Helixi91 – 933Combined sources
Helixi105 – 1084Combined sources
Helixi116 – 12813Combined sources
Helixi135 – 1373Combined sources
Helixi138 – 1469Combined sources
Helixi148 – 16316Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CYXX-ray2.56A/B/C1-165[»]
2KLYNMR-A1-165[»]
2LXPNMR-A2-165[»]
3H8KX-ray1.80A2-165[»]
4LADX-ray2.30A1-165[»]
ProteinModelPortaliP60604.
SMRiP60604. Positions 2-165.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60604.

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00730000110436.
HOGENOMiHOG000233454.
HOVERGENiHBG063308.
InParanoidiP60604.
KOiK04555.
OMAiSPFKMKF.
OrthoDBiEOG7VB2HT.
PhylomeDBiP60604.
TreeFamiTF101118.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P60604-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGTALKRLM AEYKQLTLNP PEGIVAGPMN EENFFEWEAL IMGPEDTCFE
60 70 80 90 100
FGVFPAILSF PLDYPLSPPK MRFTCEMFHP NIYPDGRVCI SILHAPGDDP
110 120 130 140 150
MGYESSAERW SPVQSVEKIL LSVVSMLAEP NDESGANVDA SKMWRDDREQ
160
FYKIAKQIVQ KSLGL
Length:165
Mass (Da):18,566
Last modified:March 14, 2004 - v1
Checksum:i74DEC732A79575E3
GO
Isoform 2 (identifier: P60604-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: Missing.

Note: No experimental confirmation available. Gene prediction based on EST data.

Show »
Length:137
Mass (Da):15,614
Checksum:iFA95FDC41C3FCAD4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121E → V in AAC32312 (PubMed:9693041).Curated
Sequence conflicti101 – 1077MGYESSA → HGLREQP in AAC32312 (PubMed:9693041).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2828Missing in isoform 2. CuratedVSP_046260Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF032456 mRNA. Translation: AAC32312.1.
BT006914 mRNA. Translation: AAP35560.1.
AK290629 mRNA. Translation: BAF83318.1.
AL163300 Genomic DNA. Translation: CAB90551.1.
CH471079 Genomic DNA. Translation: EAX09395.1.
CH471079 Genomic DNA. Translation: EAX09397.1.
CH471079 Genomic DNA. Translation: EAX09398.1.
CH471079 Genomic DNA. Translation: EAX09399.1.
BC001738 mRNA. Translation: AAH01738.1.
BC008351 mRNA. Translation: AAH08351.1.
BC011569 mRNA. Translation: AAH11569.1.
CCDSiCCDS13714.1. [P60604-1]
CCDS33586.1. [P60604-2]
RefSeqiNP_001189418.1. NM_001202489.1.
NP_003334.2. NM_003343.5. [P60604-1]
NP_872630.1. NM_182688.2. [P60604-2]
UniGeneiHs.529420.
Hs.701398.

Genome annotation databases

EnsembliENST00000330942; ENSP00000331384; ENSG00000184787. [P60604-2]
ENST00000345496; ENSP00000338348; ENSG00000184787. [P60604-1]
GeneIDi7327.
KEGGihsa:7327.
UCSCiuc002zfx.3. human. [P60604-1]

Polymorphism databases

DMDMi45593583.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF032456 mRNA. Translation: AAC32312.1.
BT006914 mRNA. Translation: AAP35560.1.
AK290629 mRNA. Translation: BAF83318.1.
AL163300 Genomic DNA. Translation: CAB90551.1.
CH471079 Genomic DNA. Translation: EAX09395.1.
CH471079 Genomic DNA. Translation: EAX09397.1.
CH471079 Genomic DNA. Translation: EAX09398.1.
CH471079 Genomic DNA. Translation: EAX09399.1.
BC001738 mRNA. Translation: AAH01738.1.
BC008351 mRNA. Translation: AAH08351.1.
BC011569 mRNA. Translation: AAH11569.1.
CCDSiCCDS13714.1. [P60604-1]
CCDS33586.1. [P60604-2]
RefSeqiNP_001189418.1. NM_001202489.1.
NP_003334.2. NM_003343.5. [P60604-1]
NP_872630.1. NM_182688.2. [P60604-2]
UniGeneiHs.529420.
Hs.701398.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CYXX-ray2.56A/B/C1-165[»]
2KLYNMR-A1-165[»]
2LXPNMR-A2-165[»]
3H8KX-ray1.80A2-165[»]
4LADX-ray2.30A1-165[»]
ProteinModelPortaliP60604.
SMRiP60604. Positions 2-165.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113175. 44 interactions.
DIPiDIP-50750N.
IntActiP60604. 24 interactions.
STRINGi9606.ENSP00000338348.

PTM databases

PhosphoSiteiP60604.

Polymorphism databases

DMDMi45593583.

Proteomic databases

MaxQBiP60604.
PaxDbiP60604.
PRIDEiP60604.

Protocols and materials databases

DNASUi7327.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000330942; ENSP00000331384; ENSG00000184787. [P60604-2]
ENST00000345496; ENSP00000338348; ENSG00000184787. [P60604-1]
GeneIDi7327.
KEGGihsa:7327.
UCSCiuc002zfx.3. human. [P60604-1]

Organism-specific databases

CTDi7327.
GeneCardsiGC21M046188.
HGNCiHGNC:12483. UBE2G2.
HPAiHPA003332.
MIMi603124. gene.
neXtProtiNX_P60604.
PharmGKBiPA37132.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00730000110436.
HOGENOMiHOG000233454.
HOVERGENiHBG063308.
InParanoidiP60604.
KOiK04555.
OMAiSPFKMKF.
OrthoDBiEOG7VB2HT.
PhylomeDBiP60604.
TreeFamiTF101118.

Enzyme and pathway databases

UniPathwayiUPA00143.
BRENDAi2.3.2.B6. 2681.
ReactomeiREACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiP60604.

Miscellaneous databases

ChiTaRSiUBE2G2. human.
EvolutionaryTraceiP60604.
GeneWikiiUBE2G2.
GenomeRNAii7327.
NextBioi28670.
PROiP60604.
SOURCEiSearch...

Gene expression databases

BgeeiP60604.
CleanExiHS_UBE2G2.
ExpressionAtlasiP60604. baseline and differential.
GenevestigatoriP60604.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification, expression, and chromosomal localization of ubiquitin conjugating enzyme 7 (UBE2G2), a human homologue of the Saccharomyces cerevisiae UBC7 gene."
    Katsanis N., Fisher E.M.C.
    Genomics 51:128-131(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Heart.
  4. "The DNA sequence of human chromosome 21."
    Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A.
    , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.
    Nature 405:311-319(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lung.
  7. "The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines."
    David Y., Ziv T., Admon A., Navon A.
    J. Biol. Chem. 285:8595-8604(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "STT3B-dependent posttranslational N-glycosylation as a surveillance system for secretory protein."
    Sato T., Sako Y., Sho M., Momohara M., Suico M.A., Shuto T., Nishitoh H., Okiyoneda T., Kokame K., Kaneko M., Taura M., Miyata M., Chosa K., Koga T., Morino-Koga S., Wada I., Kai H.
    Mol. Cell 47:99-110(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN ERAD PATHWAY.
  10. "Structure of human ubiquitin-conjugating enzyme E2 G2 (UBE2G2/UBC7)."
    Arai R., Yoshikawa S., Murayama K., Imai Y., Takahashi R., Shirouzu M., Yokoyama S.
    Acta Crystallogr. F 62:330-334(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.56 ANGSTROMS).

Entry informationi

Entry nameiUB2G2_HUMAN
AccessioniPrimary (citable) accession number: P60604
Secondary accession number(s): A6NMQ7
, A8K3L4, D3DSL7, P56554
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 14, 2004
Last sequence update: March 14, 2004
Last modified: March 31, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 21
    Human chromosome 21: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.