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Protein

Reactive oxygen species modulator 1

Gene

ROMO1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Induces production of reactive oxygen species (ROS) which are necessary for cell proliferation. May play a role in inducing oxidative DNA damage and replicative senescence. May play a role in the coordination of mitochondrial morphology and cell proliferation.
Has antibacterial activity against a variety of bacteria including S.aureus, P.aeruginosa and M.tuberculosis. Acts by inducing bacterial membrane breakage.

GO - Biological processi

  • cellular response to reactive oxygen species Source: UniProtKB
  • defense response to bacterium Source: UniProtKB-KW
  • positive regulation of cell proliferation Source: UniProtKB
  • positive regulation of reactive oxygen species metabolic process Source: UniProtKB
  • replicative cell aging Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial

Protein family/group databases

TCDBi9.B.181.1.1. the reactive oxygen species modulator 1 (romo1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Reactive oxygen species modulator 1
Short name:
ROS modulator 1
Alternative name(s):
Epididymis tissue protein Li 175
Glyrichin
Mitochondrial targeting GxxxG motif protein
Short name:
MTGM
Protein MGR2 homolog
Gene namesi
Name:ROMO1
Synonyms:C20orf52
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:16185. ROMO1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei22 – 4423HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial inner membrane Source: UniProtKB-SubCell
  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA164725540.

Polymorphism and mutation databases

BioMutaiROMO1.
DMDMi45593159.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 7978Reactive oxygen species modulator 1PRO_0000079433Add
BLAST

Proteomic databases

EPDiP60602.
MaxQBiP60602.
PaxDbiP60602.
PRIDEiP60602.
TopDownProteomicsiP60602-1. [P60602-1]

Expressioni

Tissue specificityi

Up-regulated in a number of cancer cell lines when compared to a normal lung fibroblast cell line. Highly expressed in brain tumors.2 Publications

Developmental stagei

Expression increases in senescent cells.1 Publication

Inductioni

By the anticancer drug fluorouracil (5FU).1 Publication

Gene expression databases

BgeeiP60602.
CleanExiHS_ROMO1.
GenevisibleiP60602. HS.

Organism-specific databases

HPAiHPA012782.

Interactioni

Protein-protein interaction databases

BioGridi126718. 1 interaction.
STRINGi9606.ENSP00000338293.

Structurei

3D structure databases

ProteinModelPortaliP60602.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni42 – 6019Sufficient for antibacterial activityAdd
BLAST

Sequence similaritiesi

Belongs to the MGR2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4096. Eukaryota.
ENOG411279J. LUCA.
GeneTreeiENSGT00390000005315.
HOVERGENiHBG051169.
InParanoidiP60602.
OMAiKMGFTIG.
OrthoDBiEOG7KM5WF.
PhylomeDBiP60602.
TreeFamiTF300273.

Family and domain databases

InterProiIPR018450. Romo1/Mgr2.
[Graphical view]
PfamiPF10247. Romo1. 1 hit.
[Graphical view]
SMARTiSM01378. Romo1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P60602-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPVAVGPYGQ SQPSCFDRVK MGFVMGCAVG MAAGALFGTF SCLRIGMRGR
60 70
ELMGGIGKTM MQSGGTFGTF MAIGMGIRC
Length:79
Mass (Da):8,183
Last modified:March 15, 2004 - v1
Checksum:iAD959C88C89EF80A
GO
Isoform 2 (identifier: P60602-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     44-79: RIGMRGRELMGGIGKTMMQSGGTFGTFMAIGMGIRC → SSILVSSSGSECGVES

Note: Gene prediction based on EST data.
Show »
Length:59
Mass (Da):5,900
Checksum:i22C898C2946D76C7
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti28 – 281A → P.
Corresponds to variant rs1044521 [ dbSNP | Ensembl ].
VAR_014127

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei44 – 7936RIGMR…MGIRC → SSILVSSSGSECGVES in isoform 2. CuratedVSP_036486Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM397244 mRNA. Translation: CAL37002.1.
AM397245 mRNA. Translation: CAL37003.1.
AM397246 mRNA. Translation: CAL37004.1.
GU727625 mRNA. Translation: ADU87627.1.
AL357374 Genomic DNA. Translation: CAC11117.1.
AL357374 Genomic DNA. Translation: CAI13604.1.
CH471077 Genomic DNA. Translation: EAW76168.1.
CH471077 Genomic DNA. Translation: EAW76169.1.
BC008488 mRNA. Translation: AAH08488.1.
BC105281 mRNA. Translation: AAI05282.1.
CCDSiCCDS13264.1. [P60602-1]
RefSeqiNP_542786.1. NM_080748.2. [P60602-1]
UniGeneiHs.472564.

Genome annotation databases

EnsembliENST00000336695; ENSP00000338293; ENSG00000125995. [P60602-1]
ENST00000374072; ENSP00000363185; ENSG00000125995. [P60602-2]
ENST00000374077; ENSP00000363190; ENSG00000125995. [P60602-1]
ENST00000374078; ENSP00000363191; ENSG00000125995. [P60602-1]
ENST00000397416; ENSP00000380561; ENSG00000125995. [P60602-1]
GeneIDi140823.
KEGGihsa:140823.
UCSCiuc002xdy.4. human. [P60602-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM397244 mRNA. Translation: CAL37002.1.
AM397245 mRNA. Translation: CAL37003.1.
AM397246 mRNA. Translation: CAL37004.1.
GU727625 mRNA. Translation: ADU87627.1.
AL357374 Genomic DNA. Translation: CAC11117.1.
AL357374 Genomic DNA. Translation: CAI13604.1.
CH471077 Genomic DNA. Translation: EAW76168.1.
CH471077 Genomic DNA. Translation: EAW76169.1.
BC008488 mRNA. Translation: AAH08488.1.
BC105281 mRNA. Translation: AAI05282.1.
CCDSiCCDS13264.1. [P60602-1]
RefSeqiNP_542786.1. NM_080748.2. [P60602-1]
UniGeneiHs.472564.

3D structure databases

ProteinModelPortaliP60602.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126718. 1 interaction.
STRINGi9606.ENSP00000338293.

Protein family/group databases

TCDBi9.B.181.1.1. the reactive oxygen species modulator 1 (romo1) family.

Polymorphism and mutation databases

BioMutaiROMO1.
DMDMi45593159.

Proteomic databases

EPDiP60602.
MaxQBiP60602.
PaxDbiP60602.
PRIDEiP60602.
TopDownProteomicsiP60602-1. [P60602-1]

Protocols and materials databases

DNASUi140823.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000336695; ENSP00000338293; ENSG00000125995. [P60602-1]
ENST00000374072; ENSP00000363185; ENSG00000125995. [P60602-2]
ENST00000374077; ENSP00000363190; ENSG00000125995. [P60602-1]
ENST00000374078; ENSP00000363191; ENSG00000125995. [P60602-1]
ENST00000397416; ENSP00000380561; ENSG00000125995. [P60602-1]
GeneIDi140823.
KEGGihsa:140823.
UCSCiuc002xdy.4. human. [P60602-1]

Organism-specific databases

CTDi140823.
GeneCardsiROMO1.
HGNCiHGNC:16185. ROMO1.
HPAiHPA012782.
neXtProtiNX_P60602.
PharmGKBiPA164725540.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4096. Eukaryota.
ENOG411279J. LUCA.
GeneTreeiENSGT00390000005315.
HOVERGENiHBG051169.
InParanoidiP60602.
OMAiKMGFTIG.
OrthoDBiEOG7KM5WF.
PhylomeDBiP60602.
TreeFamiTF300273.

Miscellaneous databases

ChiTaRSiROMO1. human.
GenomeRNAii140823.
PROiP60602.

Gene expression databases

BgeeiP60602.
CleanExiHS_ROMO1.
GenevisibleiP60602. HS.

Family and domain databases

InterProiIPR018450. Romo1/Mgr2.
[Graphical view]
PfamiPF10247. Romo1. 1 hit.
[Graphical view]
SMARTiSM01378. Romo1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The novel conserved mitochondrial inner-membrane protein MTGM regulates mitochondrial morphology and cell proliferation."
    Zhao J., Liu T., Jin S.B., Tomilin N., Castro J., Shupliakov O., Lendahl U., Nister M.
    J. Cell Sci. 122:2252-2262(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Tissue: Brain.
  2. "Systematic mapping and functional analysis of a family of human epididymal secretory sperm-located proteins."
    Li J., Liu F., Wang H., Liu X., Liu J., Li N., Wan F., Wang W., Zhang C., Jin S., Liu J., Zhu P., Liu Y.
    Mol. Cell. Proteomics 9:2517-2528(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Epididymis.
  3. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Bone marrow.
  6. "A novel protein, Romo1, induces ROS production in the mitochondria."
    Chung Y.M., Kim J.S., Yoo Y.D.
    Biochem. Biophys. Res. Commun. 347:649-655(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  7. "Drug resistance to 5-FU linked to reactive oxygen species modulator 1."
    Hwang I.T., Chung Y.M., Kim J.J., Chung J.S., Kim B.S., Kim H.J., Kim J.S., Yoo Y.D.
    Biochem. Biophys. Res. Commun. 359:304-310(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  8. Cited for: FUNCTION.
  9. "Replicative senescence induced by Romo1-derived reactive oxygen species."
    Chung Y.M., Lee S.B., Kim H.J., Park S.H., Kim J.J., Chung J.S., Yoo Y.D.
    J. Biol. Chem. 283:33763-33771(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Antibacterial potential of hGlyrichin encoded by a human gene."
    Sha J., Zhao G., Chen X., Guan W., He Y., Wang Z.
    J. Pept. Sci. 18:97-104(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ANTIBACTERIAL FUNCTION.
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. Cited for: CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiROMO1_HUMAN
AccessioniPrimary (citable) accession number: P60602
Secondary accession number(s): A7M872
, E1P5R9, E9KL28, Q3MHD5, Q5QP16, Q9CQ98, Q9H1N2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: March 15, 2004
Last modified: June 8, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Enforced expression in IMR-90 cells leads to increased levels of ROS and induces premature cell senescence and nuclear DNA damage.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.