P60602 (ROMO1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Reactive oxygen species modulator 1
Short name=ROS modulator 1

Alternative name(s):
Epididymis tissue protein Li 175
Glyrichin
Mitochondrial targeting GxxxG motif protein
Short name=MTGM
Protein MGR2 homolog

Gene names

Name:	ROMO1
Synonyms:	C20orf52

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	79 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Induces production of reactive oxygen species (ROS) which are necessary for cell proliferation. May play a role in inducing oxidative DNA damage and replicative senescence. May play a role in the coordination of mitochondrial morphology and cell proliferation. Ref.1 Ref.6 Ref.7 Ref.8 Ref.9 Ref.11 Has antibacterial activity against a variety of bacteria including S. aureus, P. aeruginosa and M. tuberculosis. Acts by inducing bacterial membrane breakage. Ref.1 Ref.6 Ref.7 Ref.8 Ref.9 Ref.11
Subcellular location	Mitochondrion inner membrane; Single-pass membrane protein Ref.1 Ref.6.
Tissue specificity	Up-regulated in a number of cancer cell lines when compared to a normal lung fibroblast cell line. Highly expressed in brain tumors. Ref.1 Ref.6
Developmental stage	Expression increases in senescent cells. Ref.9
Induction	By the anticancer drug fluorouracil (5FU). Ref.7
Miscellaneous	Enforced expression in IMR-90 cells leads to increased levels of ROS and induces premature cell senescence and nuclear DNA damage.
Sequence similarities	Belongs to the MGR2 family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	Antibiotic Antimicrobial
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to reactive oxygen species Inferred from mutant phenotype Ref.9. Source: UniProtKB positive regulation of cell proliferation Inferred from direct assay Ref.8. Source: UniProtKB positive regulation of reactive oxygen species metabolic process Inferred from direct assay Ref.6. Source: UniProtKB replicative cell aging Inferred from mutant phenotype Ref.9. Source: UniProtKB
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P60602-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P60602-2)

The sequence of this isoform differs from the canonical sequence as follows:
     44-79: RIGMRGRELMGGIGKTMMQSGGTFGTFMAIGMGIRC → SSILVSSSGSECGVES

Note: Gene prediction based on EST data.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 79	79	Reactive oxygen species modulator 1		PRO_0000079433
Regions
	Transmembrane	22 – 44	23	Helical; Potential
	Region	42 – 60	19	Sufficient for antibacterial activity
Natural variations
	Alternative sequence	44 – 79	36	RIGMR…MGIRC → SSILVSSSGSECGVES in isoform 2.		VSP_036486
	Natural variant	28	1	A → P. Corresponds to variant rs1044521 [ dbSNP | Ensembl ].		VAR_014127

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 15, 2004. Version 1. Checksum: AD959C88C89EF80A	FASTA	79	8,183
	10 20 30 40 50 60 MPVAVGPYGQ SQPSCFDRVK MGFVMGCAVG MAAGALFGTF SCLRIGMRGR ELMGGIGKTM 70 MQSGGTFGTF MAIGMGIRC
	Isoform 2 [UniParc]. Checksum: 22C898C2946D76C7	FASTA	59	5,900
	10 20 30 40 50 MPVAVGPYGQ SQPSCFDRVK MGFVMGCAVG MAAGALFGTF SCLSSILVSS SGSECGVES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The novel conserved mitochondrial inner-membrane protein MTGM regulates mitochondrial morphology and cell proliferation." Zhao J., Liu T., Jin S.B., Tomilin N., Castro J., Shupliakov O., Lendahl U., Nister M. J. Cell Sci. 122:2252-2262(2009) [PubMed: 19535734] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION. Tissue: Brain.
[2]	"Systematic mapping and functional analysis of a family of human epididymal secretory sperm-located proteins." Li J., Liu F., Wang H., Liu X., Liu J., Li N., Wan F., Wang W., Zhang C., Jin S., Liu J., Zhu P., Liu Y. Mol. Cell. Proteomics 9:2517-2528(2010) [PubMed: 20736409] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Epididymis.
[3]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed: 11780052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Bone marrow.
[6]	"A novel protein, Romo1, induces ROS production in the mitochondria." Chung Y.M., Kim J.S., Yoo Y.D. Biochem. Biophys. Res. Commun. 347:649-655(2006) [PubMed: 16842742] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]	"Drug resistance to 5-FU linked to reactive oxygen species modulator 1." Hwang I.T., Chung Y.M., Kim J.J., Chung J.S., Kim B.S., Kim H.J., Kim J.S., Yoo Y.D. Biochem. Biophys. Res. Commun. 359:304-310(2007) [PubMed: 17537404] [Abstract] Cited for: FUNCTION, INDUCTION.
[8]	"A critical role for Romo1-derived ROS in cell proliferation." Na A.R., Chung Y.M., Lee S.B., Park S.H., Lee M.-S., Yoo Y.D. Biochem. Biophys. Res. Commun. 369:672-678(2008) [PubMed: 18313394] [Abstract] Cited for: FUNCTION.
[9]	"Replicative senescence induced by Romo1-derived reactive oxygen species." Chung Y.M., Lee S.B., Kim H.J., Park S.H., Kim J.J., Chung J.S., Yoo Y.D. J. Biol. Chem. 283:33763-33771(2008) [PubMed: 18836179] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"Antibacterial potential of hGlyrichin encoded by a human gene." Sha J., Zhao G., Chen X., Guan W., He Y., Wang Z. J. Pept. Sci. 0:0-0(2011) [PubMed: 22083756] [Abstract] Cited for: ANTIBACTERIAL FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM397244 mRNA. Translation: CAL37002.1. AM397245 mRNA. Translation: CAL37003.1. AM397246 mRNA. Translation: CAL37004.1. GU727625 mRNA. Translation: ADU87627.1. AL357374 Genomic DNA. Translation: CAC11117.1. AL357374 Genomic DNA. Translation: CAI13604.1. CH471077 Genomic DNA. Translation: EAW76168.1. CH471077 Genomic DNA. Translation: EAW76169.1. BC008488 mRNA. Translation: AAH08488.1. BC105281 mRNA. Translation: AAI05282.1.
IPI	IPI00016046. IPI00515096.
RefSeq	NP_542786.1. NM_080748.2.
UniGene	Hs.472564.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P60602.
Polymorphism databases
DMDM	45593159.
Proteomic databases
PRIDE	P60602.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000336695; ENSP00000338293; ENSG00000125995. ENST00000374077; ENSP00000363190; ENSG00000125995. ENST00000374078; ENSP00000363191; ENSG00000125995. ENST00000397416; ENSP00000380561; ENSG00000125995.
GeneID	140823.
KEGG	hsa:140823.
UCSC	uc002xdy.1. human.
Organism-specific databases
CTD	140823.
GeneCards	GC20P034288.
H-InvDB	HIX0021258.
HGNC	HGNC:16185. ROMO1.
HPA	HPA012782.
neXtProt	NX_P60602.
PharmGKB	PA164725540.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG05112.
GeneTree	ENSGT00390000005315.
HOGENOM	HBG395519.
HOVERGEN	HBG051169.
InParanoid	P60602.
OMA	MGFAVGM.
OrthoDB	EOG4SXNF7.
PhylomeDB	P60602.
Gene expression databases
ArrayExpress	P60602.
Bgee	P60602.
CleanEx	HS_ROMO1.
Genevestigator	P60602.
GermOnline	ENSG00000125995. Homo sapiens.
Family and domain databases
InterPro	IPR018450. Romo1. [Graphical view]
Pfam	PF10247. Romo1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	84421.

Entry information

Entry name

ROMO1_HUMAN

Accession

Primary (citable) accession number: P60602
Secondary accession number(s): A7M872 , E1P5R9, E9KL28, Q3MHD5, Q5QP16, Q9CQ98, Q9H1N2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	March 15, 2004
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.