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Protein

Imidazole glycerol phosphate synthase subunit HisH

Gene

hisH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of IGP and AICAR.

Catalytic activityi

5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H2O.1 Publication

Kineticsi

  1. KM=1.5 µM for glutamine1 Publication

    pH dependencei

    Optimum pH is 6-8.1 Publication

    Pathwayi: L-histidine biosynthesis

    This protein is involved in step 5 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.
    Proteins known to be involved in the 9 steps of the subpathway in this organism are:
    1. ATP phosphoribosyltransferase (hisG)
    2. Histidine biosynthesis bifunctional protein HisIE (hisI)
    3. Histidine biosynthesis bifunctional protein HisIE (hisI)
    4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
    5. Imidazole glycerol phosphate synthase subunit HisH (hisH), Imidazole glycerol phosphate synthase subunit HisF (hisF)
    6. Histidine biosynthesis bifunctional protein HisB (hisB)
    7. Histidinol-phosphate aminotransferase (hisC)
    8. Histidine biosynthesis bifunctional protein HisB (hisB)
    9. Histidinol dehydrogenase (hisD)
    This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei77 – 771NucleophileBy similarity
    Active sitei178 – 1781By similarity
    Active sitei180 – 1801By similarity

    GO - Molecular functioni

    • imidazoleglycerol-phosphate synthase activity Source: EcoCyc

    GO - Biological processi

    • glutamine metabolic process Source: UniProtKB-HAMAP
    • histidine biosynthetic process Source: EcoCyc
    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Histidine biosynthesis

    Enzyme and pathway databases

    BioCyciEcoCyc:GLUTAMIDOTRANS-MONOMER.
    ECOL316407:JW2005-MONOMER.
    MetaCyc:GLUTAMIDOTRANS-MONOMER.
    BRENDAi4.3.1.B2. 2026.
    UniPathwayiUPA00031; UER00010.

    Protein family/group databases

    MEROPSiC26.965.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Imidazole glycerol phosphate synthase subunit HisH (EC:2.4.2.-)
    Alternative name(s):
    IGP synthase glutamine amidotransferase subunit
    IGP synthase subunit HisH
    ImGP synthase subunit HisH
    Short name:
    IGPS subunit HisH
    Gene namesi
    Name:hisH
    Ordered Locus Names:b2023, JW2005
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10450. hisH.

    Subcellular locationi

    GO - Cellular componenti

    • imidazoleglycerol-phosphate synthase complex Source: EcoCyc
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 196196Imidazole glycerol phosphate synthase subunit HisHPRO_0000152373Add
    BLAST

    Proteomic databases

    EPDiP60595.
    PaxDbiP60595.
    PRIDEiP60595.

    2D gel databases

    SWISS-2DPAGEP60595.

    Interactioni

    Subunit structurei

    Heterodimer of HisH and HisF.1 Publication

    Protein-protein interaction databases

    BioGridi4260419. 10 interactions.
    IntActiP60595. 1 interaction.
    STRINGi511145.b2023.

    Structurei

    3D structure databases

    ProteinModelPortaliP60595.
    SMRiP60595. Positions 2-196.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 196195Glutamine amidotransferase type-1Add
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiENOG4108UJE. Bacteria.
    COG0118. LUCA.
    HOGENOMiHOG000025030.
    InParanoidiP60595.
    KOiK02501.
    OMAiGMQMLLT.
    PhylomeDBiP60595.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    HAMAPiMF_00278. HisH. 1 hit.
    InterProiIPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR010139. Imidazole-glycPsynth_HisH.
    [Graphical view]
    PfamiPF00117. GATase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000495. Amidotransf_hisH. 1 hit.
    SUPFAMiSSF52317. SSF52317. 1 hit.
    TIGRFAMsiTIGR01855. IMP_synth_hisH. 1 hit.
    PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P60595-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNVVILDTGC ANLNSVKSAI ARHGYEPKVS RDPDVVLLAD KLFLPGVGTA
    60 70 80 90 100
    QAAMDQVRER ELFDLIKACT QPVLGICLGM QLLGRRSEES NGVDLLGIID
    110 120 130 140 150
    EDVPKMTDFG LPLPHMGWNR VYPQAGNRLF QGIEDGAYFY FVHSYAMPVN
    160 170 180 190
    PWTIAQCNYG EPFTAAVQKD NFYGVQFHPE RSGAAGAKLL KNFLEM
    Length:196
    Mass (Da):21,653
    Last modified:March 15, 2004 - v1
    Checksum:iB75A500ECD50DDD0
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X13462 Genomic DNA. Translation: CAA31815.1.
    U00096 Genomic DNA. Translation: AAC75084.1.
    AP009048 Genomic DNA. Translation: BAA15854.1.
    PIRiJS0132. XQECHH.
    RefSeqiNP_416527.1. NC_000913.3.
    WP_001103560.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75084; AAC75084; b2023.
    BAA15854; BAA15854; BAA15854.
    GeneIDi946544.
    KEGGiecj:JW2005.
    eco:b2023.
    PATRICi32119381. VBIEscCol129921_2100.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X13462 Genomic DNA. Translation: CAA31815.1.
    U00096 Genomic DNA. Translation: AAC75084.1.
    AP009048 Genomic DNA. Translation: BAA15854.1.
    PIRiJS0132. XQECHH.
    RefSeqiNP_416527.1. NC_000913.3.
    WP_001103560.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP60595.
    SMRiP60595. Positions 2-196.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4260419. 10 interactions.
    IntActiP60595. 1 interaction.
    STRINGi511145.b2023.

    Protein family/group databases

    MEROPSiC26.965.

    2D gel databases

    SWISS-2DPAGEP60595.

    Proteomic databases

    EPDiP60595.
    PaxDbiP60595.
    PRIDEiP60595.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC75084; AAC75084; b2023.
    BAA15854; BAA15854; BAA15854.
    GeneIDi946544.
    KEGGiecj:JW2005.
    eco:b2023.
    PATRICi32119381. VBIEscCol129921_2100.

    Organism-specific databases

    EchoBASEiEB0445.
    EcoGeneiEG10450. hisH.

    Phylogenomic databases

    eggNOGiENOG4108UJE. Bacteria.
    COG0118. LUCA.
    HOGENOMiHOG000025030.
    InParanoidiP60595.
    KOiK02501.
    OMAiGMQMLLT.
    PhylomeDBiP60595.

    Enzyme and pathway databases

    UniPathwayiUPA00031; UER00010.
    BioCyciEcoCyc:GLUTAMIDOTRANS-MONOMER.
    ECOL316407:JW2005-MONOMER.
    MetaCyc:GLUTAMIDOTRANS-MONOMER.
    BRENDAi4.3.1.B2. 2026.

    Miscellaneous databases

    PROiP60595.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    HAMAPiMF_00278. HisH. 1 hit.
    InterProiIPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR010139. Imidazole-glycPsynth_HisH.
    [Graphical view]
    PfamiPF00117. GATase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000495. Amidotransf_hisH. 1 hit.
    SUPFAMiSSF52317. SSF52317. 1 hit.
    TIGRFAMsiTIGR01855. IMP_synth_hisH. 1 hit.
    PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiHIS5_ECOLI
    AccessioniPrimary (citable) accession number: P60595
    Secondary accession number(s): P10375
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2004
    Last sequence update: March 15, 2004
    Last modified: September 7, 2016
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.