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Protein

Omega-theraphotoxin-Gr1a

Gene
N/A
Organism
Grammostola rosea (Chilean rose tarantula) (Grammostola spatulata)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits P/Q- (Cav2.1/CACNA1A) and N-type (Cav2.2/CACNA1B) voltage-gated calcium channel by modifying voltage-dependent gating. It selectively and reversibly blocks the calcium channels coupled to glutamate release. Also inhibits potassium channels (Kv2.1/KCNB1) with lower affinity.5 Publications

GO - Molecular functioni

  • calcium channel inhibitor activity Source: CACAO
  • potassium channel inhibitor activity Source: CACAO

GO - Biological processi

Keywordsi

Molecular functionCalcium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin, Voltage-gated calcium channel impairing toxin, Voltage-gated potassium channel impairing toxin

Protein family/group databases

TCDBi8.B.5.3.7. the na(+)/k(+)/ca(2+) channel targeting tarantula huwentoxin (tht) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Omega-theraphotoxin-Gr1a
Short name:
Omega-TRTX-Gr1a
Alternative name(s):
Omega-GTX SIA
Omega-GsTx SIA
Omega-grammotoxin SIA
Short name:
Omega-GrTx SIA
OrganismiGrammostola rosea (Chilean rose tarantula) (Grammostola spatulata)
Taxonomic identifieri432528 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeTheraphosidaeGrammostola

Organism-specific databases

ArachnoServeriAS000426. omega-theraphotoxin-Gr1a.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000041429725 – 492 PublicationsAdd BLAST25
PeptideiPRO_000004501550 – 85Omega-theraphotoxin-Gr1aAdd BLAST36

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi51 ↔ 651 Publication
Disulfide bondi58 ↔ 701 Publication
Disulfide bondi64 ↔ 791 Publication
Modified residuei85Valine amideBy similarity1

Keywords - PTMi

Amidation, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

187
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi60 – 64Combined sources5
Beta strandi68 – 70Combined sources3
Beta strandi73 – 78Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KOZNMR-A50-85[»]
SMRiP60590.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60590.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Belongs to the huwentoxin-1 family. GrTx subfamily.Curated

Keywords - Domaini

Knottin, Signal

Family and domain databases

InterProiView protein in InterPro
IPR011696. Huwentoxin-1.
PfamiView protein in Pfam
PF07740. Toxin_12. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60590-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAQIFVVVL GLAALSVLCY GSEADESALH EEIFQLLAAS DEVPKPQERD
60 70 80
CVRFWGKCSQ TSDCCPHLAC KSKWPRNICV WDGSVGK
Length:87
Mass (Da):9,601
Last modified:December 14, 2011 - v2
Checksum:i5A2B2FC706847B51
GO

Mass spectrometryi

Molecular mass is 4109.2 Da from positions 50 - 85. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB612243 mRNA. Translation: BAK55735.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB612243 mRNA. Translation: BAK55735.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KOZNMR-A50-85[»]
SMRiP60590.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi8.B.5.3.7. the na(+)/k(+)/ca(2+) channel targeting tarantula huwentoxin (tht) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ArachnoServeriAS000426. omega-theraphotoxin-Gr1a.

Miscellaneous databases

EvolutionaryTraceiP60590.

Family and domain databases

InterProiView protein in InterPro
IPR011696. Huwentoxin-1.
PfamiView protein in Pfam
PF07740. Toxin_12. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiWGRTX_GRARO
AccessioniPrimary (citable) accession number: P60590
Secondary accession number(s): F8WQV8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: December 14, 2011
Last modified: November 2, 2016
This is version 62 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.