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Protein

Crotonobetainyl-CoA reductase

Gene

caiA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA (PubMed:8060125, PubMed:10209289, PubMed:10978161). The electron donor could be the FixA/FixB complex (PubMed:12081978).2 Publications2 Publications

Catalytic activityi

Gamma-butyrobetainyl-CoA + electron-transfer flavoprotein = crotonobetainyl-CoA + reduced electron-transfer flavoprotein.UniRule annotation1 Publication

Cofactori

FADUniRule annotation1 PublicationNote: Binds 1 FAD per subunit.1 Publication

pH dependencei

Optimum pH is 7.8.1 Publication

Temperature dependencei

Optimum temperature is 40-45 degrees Celsius.1 Publication

Pathwayi: carnitine metabolism

This protein is involved in the pathway carnitine metabolism, which is part of Amine and polyamine metabolism.UniRule annotationCurated
View all proteins of this organism that are known to be involved in the pathway carnitine metabolism and in Amine and polyamine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein

Enzyme and pathway databases

BioCyciEcoCyc:CROBETREDUCT-MONOMER.
MetaCyc:CROBETREDUCT-MONOMER.
UniPathwayiUPA00117.

Names & Taxonomyi

Protein namesi
Recommended name:
Crotonobetainyl-CoA reductaseUniRule annotation (EC:1.3.8.13UniRule annotation1 Publication)
Alternative name(s):
Crotobetaine reductase1 Publication
Crotonobetainyl-CoA dehydrogenaseUniRule annotation
Gene namesi
Name:caiA1 PublicationUniRule annotation
Synonyms:yaaO
Ordered Locus Names:b0039, JW0038
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11560. caiA.

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Deletion of the gene abolishes gamma-butyrobetaine formation and increases L-carnitine production under anaerobic and aerobic conditions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002011931 – 380Crotonobetainyl-CoA reductaseAdd BLAST380

Proteomic databases

PaxDbiP60584.
PRIDEiP60584.

Expressioni

Inductioni

Transcribed during anaerobic growth in the presence of L-carnitine or crotonobetaine.2 Publications

Interactioni

Subunit structurei

Homotetramer.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4263349. 362 interactors.
DIPiDIP-9241N.
IntActiP60584. 5 interactors.
STRINGi316385.ECDH10B_0040.

Structurei

3D structure databases

ProteinModelPortaliP60584.
SMRiP60584.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acyl-CoA dehydrogenase family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105C1G. Bacteria.
COG1960. LUCA.
HOGENOMiHOG000131659.
InParanoidiP60584.
KOiK08297.
PhylomeDBiP60584.

Family and domain databases

Gene3Di1.10.540.10. 1 hit.
HAMAPiMF_01052. CaiA. 1 hit.
InterProiView protein in InterPro
IPR006089. Acyl-CoA_DH_CS.
IPR006091. Acyl-CoA_Oxase/DH_cen-dom.
IPR009075. AcylCo_DH/oxidase_C.
IPR013786. AcylCoA_DH/ox_N.
IPR009100. AcylCoA_DH/oxidase_NM_dom.
IPR023450. CaiA.
PfamiView protein in Pfam
PF00441. Acyl-CoA_dh_1. 1 hit.
PF02770. Acyl-CoA_dh_M. 1 hit.
PF02771. Acyl-CoA_dh_N. 1 hit.
SUPFAMiSSF47203. SSF47203. 1 hit.
SSF56645. SSF56645. 1 hit.
PROSITEiView protein in PROSITE
PS00072. ACYL_COA_DH_1. 1 hit.
PS00073. ACYL_COA_DH_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P60584-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFNLNDEQE LFVAGIRELM ASENWEAYFA ECDRDSVYPE RFVKALADMG
60 70 80 90 100
IDSLLIPEEH GGLDAGFVTL AAVWMELGRL GAPTYVLYQL PGGFNTFLRE
110 120 130 140 150
GTQEQIDKIM AFRGTGKQMW NSAITEPGAG SDVGSLKTTY TRRNGKIYLN
160 170 180 190 200
GSKCFITSSA YTPYIVVMAR DGASPDKPVY TEWFVDMSKP GIKVTKLEKL
210 220 230 240 250
GLRMDSCCEI TFDDVELDEK DMFGREGNGF NRVKEEFDHE RFLVALTNYG
260 270 280 290 300
TAMCAFEDAA RYANQRVQFG EAIGRFQLIQ EKFAHMAIKL NSMKNMLYEA
310 320 330 340 350
AWKADNGTIT SGDAAMCKYF CANAAFEVVD SAMQVLGGVG IAGNHRISRF
360 370 380
WRDLRVDRVS GGSDEMQILT LGRAVLKQYR
Length:380
Mass (Da):42,558
Last modified:March 15, 2004 - v1
Checksum:i7076984D735652C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73904 Genomic DNA. Translation: CAA52111.1.
U00096 Genomic DNA. Translation: AAC73150.1.
AP009048 Genomic DNA. Translation: BAB96608.2.
PIRiG64724. I41011.
RefSeqiNP_414581.1. NC_000913.3.
WP_000347117.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73150; AAC73150; b0039.
BAB96608; BAB96608; BAB96608.
GeneIDi949064.
KEGGiecj:JW0038.
eco:b0039.
PATRICifig|1411691.4.peg.2244.

Similar proteinsi

Entry informationi

Entry nameiCAIA_ECOLI
AccessioniPrimary (citable) accession number: P60584
Secondary accession number(s): P31571
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: March 15, 2004
Last modified: September 27, 2017
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families