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Protein

Crotonobetainyl-CoA dehydrogenase

Gene

caiA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA.

Cofactori

FAD1 PublicationNote: Binds 1 FAD per subunit.1 Publication

Pathwayi: carnitine metabolism

This protein is involved in the pathway carnitine metabolism, which is part of Amine and polyamine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway carnitine metabolism and in Amine and polyamine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciEcoCyc:CROBETREDUCT-MONOMER.
ECOL316407:JW0038-MONOMER.
UniPathwayiUPA00117.

Names & Taxonomyi

Protein namesi
Recommended name:
Crotonobetainyl-CoA dehydrogenaseUniRule annotation (EC:1.3.99.-UniRule annotation)
Alternative name(s):
Crotonobetainyl-CoA reductaseUniRule annotation
Gene namesi
Name:caiAUniRule annotation
Synonyms:yaaO
Ordered Locus Names:b0039, JW0038
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11560. caiA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 380380Crotonobetainyl-CoA dehydrogenasePRO_0000201193Add
BLAST

Proteomic databases

PaxDbiP60584.
PRIDEiP60584.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4263349. 362 interactions.
DIPiDIP-9241N.
IntActiP60584. 5 interactions.
STRINGi511145.b0039.

Structurei

3D structure databases

ProteinModelPortaliP60584.
SMRiP60584. Positions 3-377.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acyl-CoA dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C1G. Bacteria.
COG1960. LUCA.
HOGENOMiHOG000131659.
InParanoidiP60584.
KOiK08297.
OMAiHRDEYPT.
OrthoDBiEOG6J48HZ.
PhylomeDBiP60584.

Family and domain databases

Gene3Di1.10.540.10. 1 hit.
HAMAPiMF_01052. CaiA.
InterProiIPR006089. Acyl-CoA_DH_CS.
IPR006091. Acyl-CoA_Oxase/DH_cen-dom.
IPR009075. AcylCo_DH/oxidase_C.
IPR013786. AcylCoA_DH/ox_N.
IPR009100. AcylCoA_DH/oxidase_NM_dom.
IPR023450. Crotonobetainyl-CoA_DHase_CaiA.
[Graphical view]
PfamiPF00441. Acyl-CoA_dh_1. 1 hit.
PF02770. Acyl-CoA_dh_M. 1 hit.
PF02771. Acyl-CoA_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF47203. SSF47203. 1 hit.
SSF56645. SSF56645. 1 hit.
PROSITEiPS00072. ACYL_COA_DH_1. 1 hit.
PS00073. ACYL_COA_DH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P60584-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFNLNDEQE LFVAGIRELM ASENWEAYFA ECDRDSVYPE RFVKALADMG
60 70 80 90 100
IDSLLIPEEH GGLDAGFVTL AAVWMELGRL GAPTYVLYQL PGGFNTFLRE
110 120 130 140 150
GTQEQIDKIM AFRGTGKQMW NSAITEPGAG SDVGSLKTTY TRRNGKIYLN
160 170 180 190 200
GSKCFITSSA YTPYIVVMAR DGASPDKPVY TEWFVDMSKP GIKVTKLEKL
210 220 230 240 250
GLRMDSCCEI TFDDVELDEK DMFGREGNGF NRVKEEFDHE RFLVALTNYG
260 270 280 290 300
TAMCAFEDAA RYANQRVQFG EAIGRFQLIQ EKFAHMAIKL NSMKNMLYEA
310 320 330 340 350
AWKADNGTIT SGDAAMCKYF CANAAFEVVD SAMQVLGGVG IAGNHRISRF
360 370 380
WRDLRVDRVS GGSDEMQILT LGRAVLKQYR
Length:380
Mass (Da):42,558
Last modified:March 15, 2004 - v1
Checksum:i7076984D735652C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73904 Genomic DNA. Translation: CAA52111.1.
U00096 Genomic DNA. Translation: AAC73150.1.
AP009048 Genomic DNA. Translation: BAB96608.2.
PIRiG64724. I41011.
RefSeqiNP_414581.1. NC_000913.3.
WP_000347117.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73150; AAC73150; b0039.
BAB96608; BAB96608; BAB96608.
GeneIDi949064.
KEGGiecj:JW0038.
eco:b0039.
PATRICi32115173. VBIEscCol129921_0038.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73904 Genomic DNA. Translation: CAA52111.1.
U00096 Genomic DNA. Translation: AAC73150.1.
AP009048 Genomic DNA. Translation: BAB96608.2.
PIRiG64724. I41011.
RefSeqiNP_414581.1. NC_000913.3.
WP_000347117.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP60584.
SMRiP60584. Positions 3-377.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263349. 362 interactions.
DIPiDIP-9241N.
IntActiP60584. 5 interactions.
STRINGi511145.b0039.

Proteomic databases

PaxDbiP60584.
PRIDEiP60584.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73150; AAC73150; b0039.
BAB96608; BAB96608; BAB96608.
GeneIDi949064.
KEGGiecj:JW0038.
eco:b0039.
PATRICi32115173. VBIEscCol129921_0038.

Organism-specific databases

EchoBASEiEB1521.
EcoGeneiEG11560. caiA.

Phylogenomic databases

eggNOGiENOG4105C1G. Bacteria.
COG1960. LUCA.
HOGENOMiHOG000131659.
InParanoidiP60584.
KOiK08297.
OMAiHRDEYPT.
OrthoDBiEOG6J48HZ.
PhylomeDBiP60584.

Enzyme and pathway databases

UniPathwayiUPA00117.
BioCyciEcoCyc:CROBETREDUCT-MONOMER.
ECOL316407:JW0038-MONOMER.

Miscellaneous databases

PROiP60584.

Family and domain databases

Gene3Di1.10.540.10. 1 hit.
HAMAPiMF_01052. CaiA.
InterProiIPR006089. Acyl-CoA_DH_CS.
IPR006091. Acyl-CoA_Oxase/DH_cen-dom.
IPR009075. AcylCo_DH/oxidase_C.
IPR013786. AcylCoA_DH/ox_N.
IPR009100. AcylCoA_DH/oxidase_NM_dom.
IPR023450. Crotonobetainyl-CoA_DHase_CaiA.
[Graphical view]
PfamiPF00441. Acyl-CoA_dh_1. 1 hit.
PF02770. Acyl-CoA_dh_M. 1 hit.
PF02771. Acyl-CoA_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF47203. SSF47203. 1 hit.
SSF56645. SSF56645. 1 hit.
PROSITEiPS00072. ACYL_COA_DH_1. 1 hit.
PS00073. ACYL_COA_DH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of the cai operon necessary for carnitine metabolism in Escherichia coli."
    Eichler K., Bourgis F., Buchet A., Kleber H.-P., Mandrand-Berthelot M.-A.
    Mol. Microbiol. 13:775-786(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: O44:K74.
  2. "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region."
    Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A.
    Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 143 AND 182.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Crotonobetaine reductase from Escherichia coli consists of two proteins."
    Preusser A., Wagner U., Elssner T., Kleber H.-P.
    Biochim. Biophys. Acta 1431:166-178(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-20, COFACTOR, SUBUNIT.

Entry informationi

Entry nameiCAIA_ECOLI
AccessioniPrimary (citable) accession number: P60584
Secondary accession number(s): P31571
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: March 15, 2004
Last modified: January 20, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.