P60566 (FIXA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein fixA

Gene names

Name:	fixA
Synonyms:	yaaQ
Ordered Locus Names:	b0041, JW0040

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for anaerobic carnitine reduction. May bring reductant to CaiA. Ref.5
Pathway	Amine and polyamine metabolism; carnitine metabolism. HAMAP MF_01055
Subunit structure	Heterodimer of fixA and fixB Probable.
Sequence similarities	Belongs to the ETF beta-subunit/fixA family.

Ontologies

Keywords
Biological process	Electron transport Transport
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carnitine metabolic process Inferred from mutant phenotype Ref.5. Source: EcoliWiki electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	Protein fixA HAMAP MF_01055		PRO_0000167892
Experimental info
	Sequence conflict	216 – 217	2	EA → VD in CAA50797. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P60566 [UniParc]. Last modified March 15, 2004. Version 1. Checksum: 1954E0469748D310	FASTA	256	27,144
	10 20 30 40 50 60 MKIITCYKCV PDEQDIAVNN ADGSLDFSKA DAKISQYDLN AIEAACQLKQ QAAEAQVTAL 70 80 90 100 110 120 SVGGKALTNA KGRKDVLSRG PDELIVVIDD QFEQALPQQT ASALAAAAQK AGFDLILCGD 130 140 150 160 170 180 GSSDLYAQQV GLLVGEILNI PAVNGVSKII SLTADTLTVE RELEDETETL SIPLPAVVAV 190 200 210 220 230 240 STDINSPQIP SMKAILGAAK KPVQVWSAAD IGFNAEAAWS EQQVAAPKQR ERQRIVIEGD 250 GEEQIAAFAE NLRKVI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The fix Escherichia coli region contains four genes related to carnitine metabolism." Eichler K., Buchet A., Bourgis F., Kleber H.-P., Mandrand-Berthelot M.-A. J. Basic Microbiol. 35:217-227(1995) [PubMed: 7473063] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: O44:K74.
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 80. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The fixA and fixB genes are necessary for anaerobic carnitine reduction in Escherichia coli." Walt A., Kahn M.L. J. Bacteriol. 184:4044-4047(2002) [PubMed: 12081978] [Abstract] Cited for: FUNCTION. Strain: K12 / BW25113.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X71977 Genomic DNA. Translation: CAA50797.1. U00096 Genomic DNA. Translation: AAC73152.2. AP009048 Genomic DNA. Translation: BAB96610.2.
PIR	A64725.
RefSeq	NP_414583.2. NC_000913.2.
3D structure databases
ProteinModelPortal	P60566.
SMR	P60566. Positions 1-255.
ModBase	Search...
Protein-protein interaction databases
IntAct	P60566. 5 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000003622; EBESCP00000003622; EBESCG00000002955. EBESCT00000015333; EBESCP00000014624; EBESCG00000014393.
GeneID	947316.
GenomeReviews	Gene locus JW0040 in contig AP009048_GR. Gene locus b0041 in contig U00096_GR.
KEGG	ecj:JW0040. eco:b0041.
PATRIC	32115181. VBIEscCol129921_0042.
Organism-specific databases
EchoBASE	EB1523.
EcoGene	EG11562. fixA.
Phylogenomic databases
eggNOG	COG2086.
GeneTree	EBGT00050000010718.
HOGENOM	HBG716515.
OMA	ARKDILS.
PhylomeDB	P60566.
ProtClustDB	PRK03359.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11562-MONOMER.
Gene expression databases
Genevestigator	P60566.
Family and domain databases
HAMAP	MF_01055. FixA. [Tree]
InterPro	IPR000049. ET-Flavoprotein_bsu_CS. IPR014730. ETF_a/b_N. IPR012255. ETF_b. IPR023463. ETF_FixAsu_pred. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K03521.
PANTHER	PTHR21294. Beta-ETF. 1 hit.
Pfam	PF01012. ETF. 1 hit. [Graphical view]
PIRSF	PIRSF000090. Beta-ETF. 1 hit.
SMART	SM00893. ETF. 1 hit. [Graphical view]
PROSITE	PS01065. ETF_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FIXA_ECOLI

Accession

Primary (citable) accession number: P60566
Secondary accession number(s): P31573 , P75625, P76901, Q8XA29

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	March 15, 2004
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program