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Protein

Protein FixA

Gene

fixA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for anaerobic carnitine reduction. May bring reductant to CaiA.1 Publication

Pathwayi: carnitine metabolism

This protein is involved in the pathway carnitine metabolism, which is part of Amine and polyamine metabolism.
View all proteins of this organism that are known to be involved in the pathway carnitine metabolism and in Amine and polyamine metabolism.

GO - Molecular functioni

GO - Biological processi

  • carnitine metabolic process Source: EcoliWiki
  • oxidation-reduction process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11562-MONOMER.
ECOL316407:JW0040-MONOMER.
UniPathwayiUPA00117.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein FixA
Gene namesi
Name:fixA
Synonyms:yaaQ
Ordered Locus Names:b0041, JW0040
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11562. fixA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 256256Protein FixAPRO_0000167892Add
BLAST

Proteomic databases

PaxDbiP60566.

Interactioni

Subunit structurei

Heterodimer of FixA and FixB.Curated

Binary interactionsi

WithEntry#Exp.IntActNotes
ybhFP0A9U13EBI-1113126,EBI-547696

Protein-protein interaction databases

BioGridi4262207. 10 interactions.
IntActiP60566. 8 interactions.
STRINGi511145.b0041.

Structurei

3D structure databases

ProteinModelPortaliP60566.
SMRiP60566. Positions 1-246.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ETF beta-subunit/FixA family.Curated

Phylogenomic databases

eggNOGiENOG4105BZJ. Bacteria.
COG2086. LUCA.
HOGENOMiHOG000247880.
InParanoidiP60566.
KOiK03521.
OMAiREALCMG.
OrthoDBiEOG6ZSP5X.
PhylomeDBiP60566.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01055. FixA.
InterProiIPR000049. ET-Flavoprotein_bsu_CS.
IPR014730. ETF_a/b_N.
IPR012255. ETF_b.
IPR023463. FixA.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR21294. PTHR21294. 1 hit.
PfamiPF01012. ETF. 1 hit.
[Graphical view]
PIRSFiPIRSF000090. Beta-ETF. 1 hit.
SMARTiSM00893. ETF. 1 hit.
[Graphical view]
PROSITEiPS01065. ETF_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P60566-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIITCYKCV PDEQDIAVNN ADGSLDFSKA DAKISQYDLN AIEAACQLKQ
60 70 80 90 100
QAAEAQVTAL SVGGKALTNA KGRKDVLSRG PDELIVVIDD QFEQALPQQT
110 120 130 140 150
ASALAAAAQK AGFDLILCGD GSSDLYAQQV GLLVGEILNI PAVNGVSKII
160 170 180 190 200
SLTADTLTVE RELEDETETL SIPLPAVVAV STDINSPQIP SMKAILGAAK
210 220 230 240 250
KPVQVWSAAD IGFNAEAAWS EQQVAAPKQR ERQRIVIEGD GEEQIAAFAE

NLRKVI
Length:256
Mass (Da):27,144
Last modified:March 15, 2004 - v1
Checksum:i1954E0469748D310
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti216 – 2172EA → VD in CAA50797 (PubMed:7473063).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71977 Genomic DNA. Translation: CAA50797.1.
U00096 Genomic DNA. Translation: AAC73152.2.
AP009048 Genomic DNA. Translation: BAB96610.2.
PIRiA64725.
RefSeqiNP_414583.2. NC_000913.3.
WP_000692204.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73152; AAC73152; b0041.
BAB96610; BAB96610; BAB96610.
GeneIDi947316.
KEGGiecj:JW0040.
eco:b0041.
PATRICi32115181. VBIEscCol129921_0042.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71977 Genomic DNA. Translation: CAA50797.1.
U00096 Genomic DNA. Translation: AAC73152.2.
AP009048 Genomic DNA. Translation: BAB96610.2.
PIRiA64725.
RefSeqiNP_414583.2. NC_000913.3.
WP_000692204.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP60566.
SMRiP60566. Positions 1-246.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262207. 10 interactions.
IntActiP60566. 8 interactions.
STRINGi511145.b0041.

Proteomic databases

PaxDbiP60566.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73152; AAC73152; b0041.
BAB96610; BAB96610; BAB96610.
GeneIDi947316.
KEGGiecj:JW0040.
eco:b0041.
PATRICi32115181. VBIEscCol129921_0042.

Organism-specific databases

EchoBASEiEB1523.
EcoGeneiEG11562. fixA.

Phylogenomic databases

eggNOGiENOG4105BZJ. Bacteria.
COG2086. LUCA.
HOGENOMiHOG000247880.
InParanoidiP60566.
KOiK03521.
OMAiREALCMG.
OrthoDBiEOG6ZSP5X.
PhylomeDBiP60566.

Enzyme and pathway databases

UniPathwayiUPA00117.
BioCyciEcoCyc:EG11562-MONOMER.
ECOL316407:JW0040-MONOMER.

Miscellaneous databases

PROiP60566.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01055. FixA.
InterProiIPR000049. ET-Flavoprotein_bsu_CS.
IPR014730. ETF_a/b_N.
IPR012255. ETF_b.
IPR023463. FixA.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR21294. PTHR21294. 1 hit.
PfamiPF01012. ETF. 1 hit.
[Graphical view]
PIRSFiPIRSF000090. Beta-ETF. 1 hit.
SMARTiSM00893. ETF. 1 hit.
[Graphical view]
PROSITEiPS01065. ETF_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The fix Escherichia coli region contains four genes related to carnitine metabolism."
    Eichler K., Buchet A., Bourgis F., Kleber H.-P., Mandrand-Berthelot M.-A.
    J. Basic Microbiol. 35:217-227(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: O44:K74.
  2. "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region."
    Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A.
    Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 80.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The fixA and fixB genes are necessary for anaerobic carnitine reduction in Escherichia coli."
    Walt A., Kahn M.L.
    J. Bacteriol. 184:4044-4047(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: K12 / BW25113.

Entry informationi

Entry nameiFIXA_ECOLI
AccessioniPrimary (citable) accession number: P60566
Secondary accession number(s): P31573
, P75625, P76901, Q8XA29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: March 15, 2004
Last modified: January 20, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.