Reviewed, UniProtKB/Swiss-Prot P60565 (GUAC_LACJO)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	LJ_0441

Organism

Lactobacillus johnsonii [Complete proteome] [HAMAP]

Taxonomic identifier

33959 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity.
Catalytic activity	Inosine 5'-phosphate + NH(3) + NADP(+) = guanosine 5'-phosphate + NADPH.
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: InterPro purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	GMP reductase		PRO_0000093759
Regions
	Nucleotide binding	209 – 232	24	NADP By similarity
Sites
	Active site	180	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P60565-1 [UniParc]. Last modified March 15, 2004. Version 1. Checksum: 39E5484AB0C831DA	FASTA	330	36,625
	10 20 30 40 50 60 MSNYFSMEAF DYDDIQLVPN KCIIKSRSEA DTGVKFGSRT FKIPVVPANM ESVIDEDLAI 70 80 90 100 110 120 WLAEHGYYYV MHRFYPEKRA DFIKMMHDKG LFASISVGIK DSEYDFIDYL AKENIIPEYT 130 140 150 160 170 180 TIDVAHGHSD YVIKMIKYIK EKLPDTFLTA GNIATPEAVR ELENAGADAT KVGVGPGRAC 190 200 210 220 230 240 ITKLKTGFGT GGWQLAALRM CSKAARKPLI ADGGIRHNGD IAKSVRFGAS MVMIGSLFAG 250 260 270 280 290 300 HEESPGNLIT IDGKRYKQYW GSASEVQKGA YRNVEGKQML VPYRGSIKDT LREMQEDLQS 310 320 330 SISYAGGKDL SAIRVVDYVI VKSSIFDGDK

References

[1]

"The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533." Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C., Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R., Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A. Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004) [PubMed: 14983040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 533.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017198 Genomic DNA. Translation: AAS08432.1.
RefSeq	NP_964466.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2742024.
GenomeReviews	Gene locus LJ_0441 in contig AE017198_GR.
KEGG	ljo:LJ0441.
NMPDR	fig|257314.1.peg.452.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P60565.
Enzyme and pathway databases
BioCyc	LJOH257314:LJ_0441-MON.
Family and domain databases
HAMAP	MF_01511. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DHase_GMPRtase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_LACJO

Accession

Primary (citable) accession number: P60565

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	March 15, 2004
Last modified:	November 25, 2008
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)