UniProtKB - P60560 (GUAC_ECOLI)
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Protein
GMP reductase
Gene
guaC
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides.
Catalytic activityi
Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 181 | Potassium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 183 | Potassium; via carbonyl oxygenBy similarity | 1 | |
Active sitei | 186 | Thioimidate intermediateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 108 – 131 | NADPBy similarityAdd BLAST | 24 | |
Nucleotide bindingi | 216 – 239 | NADPBy similarityAdd BLAST | 24 |
GO - Molecular functioni
- GMP reductase activity Source: EcoCyc
- identical protein binding Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- purine ribonucleotide interconversion Source: EcoCyc
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Metal-binding, NADP, Potassium |
Enzyme and pathway databases
BioCyci | EcoCyc:GMP-REDUCT-MONOMER. MetaCyc:GMP-REDUCT-MONOMER. |
Names & Taxonomyi
Protein namesi | Recommended name: GMP reductase (EC:1.7.1.7)Alternative name(s): Guanosine 5'-monophosphate oxidoreductase Short name: Guanosine monophosphate reductase |
Gene namesi | Name:guaC Ordered Locus Names:b0104, JW0101 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Organism-specific databases
EcoGenei | EG10422. guaC. |
Subcellular locationi
GO - Cellular componenti
- cytosol Source: EcoCyc
- GMP reductase complex Source: UniProtKB-EC
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000093735 | 1 – 347 | GMP reductaseAdd BLAST | 347 |
Proteomic databases
EPDi | P60560. |
PaxDbi | P60560. |
PRIDEi | P60560. |
Interactioni
Subunit structurei
Homotetramer.
Binary interactionsi
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGridi | 4261108. 14 interactors. |
DIPi | DIP-47861N. |
IntActi | P60560. 6 interactors. |
STRINGi | 316385.ECDH10B_0085. |
Structurei
3D structure databases
ProteinModelPortali | P60560. |
SMRi | P60560. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG4105CP4. Bacteria. COG0516. LUCA. |
HOGENOMi | HOG000165756. |
InParanoidi | P60560. |
KOi | K00364. |
OMAi | AKVLCKF. |
PhylomeDBi | P60560. |
Family and domain databases
CDDi | cd00381. IMPDH. 1 hit. |
Gene3Di | 3.20.20.70. 1 hit. |
HAMAPi | MF_00596. GMP_reduct_type1. 1 hit. |
InterProi | View protein in InterPro IPR013785. Aldolase_TIM. IPR005993. GMPR. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. |
Pfami | View protein in Pfam PF00478. IMPDH. 1 hit. |
PIRSFi | PIRSF000235. GMP_reductase. 1 hit. |
TIGRFAMsi | TIGR01305. GMP_reduct_1. 1 hit. |
PROSITEi | View protein in PROSITE PS00487. IMP_DH_GMP_RED. 1 hit. |
i Sequence
Sequence statusi: Complete.
P60560-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRIEEDLKLG FKDVLIRPKR STLKSRSDVE LERQFTFKHS GQSWSGVPII
60 70 80 90 100
AANMDTVGTF SMASALASFD ILTAVHKHYS VEEWQAFINN SSADVLKHVM
110 120 130 140 150
VSTGTSDADF EKTKQILDLN PALNFVCIDV ANGYSEHFVQ FVAKAREAWP
160 170 180 190 200
TKTICAGNVV TGEMCEELIL SGADIVKVGI GPGSVCTTRV KTGVGYPQLS
210 220 230 240 250
AVIECADAAH GLGGMIVSDG GCTTPGDVAK AFGGGADFVM LGGMLAGHEE
260 270 280 290 300
SGGRIVEENG EKFMLFYGMS SESAMKRHVG GVAEYRAAEG KTVKLPLRGP
310 320 330 340
VENTARDILG GLRSACTYVG ASRLKELTKR TTFIRVQEQE NRIFNNL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 233 – 235 | GGG → AR (PubMed:2904262).Curated | 3 | |
Sequence conflicti | 233 – 235 | GGG → AR (PubMed:8202364).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X07917 Genomic DNA. Translation: CAA30751.1. U00096 Genomic DNA. Translation: AAC73215.1. AP009048 Genomic DNA. Translation: BAB96673.2. L28105 Genomic DNA. Translation: AAC36926.1. |
PIRi | H64732. |
RefSeqi | NP_414646.1. NC_000913.3. WP_001217338.1. NZ_LN832404.1. |
Genome annotation databases
EnsemblBacteriai | AAC73215; AAC73215; b0104. BAB96673; BAB96673; BAB96673. |
GeneIDi | 948986. |
KEGGi | ecj:JW0101. eco:b0104. |
PATRICi | fig|1411691.4.peg.2177. |
Similar proteinsi
Entry informationi
Entry namei | GUAC_ECOLI | |
Accessioni | P60560Primary (citable) accession number: P60560 Secondary accession number(s): P15344, P78048 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2004 |
Last sequence update: | March 15, 2004 | |
Last modified: | March 28, 2018 | |
This is version 119 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |