Reviewed,
UniProtKB/Swiss-Prot P60560 (GUAC_ECOLI)
Last modified
November 25, 2008.
Version 49.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: GMP reductase EC=1.7.1.7 Alternative name(s): Guanosine 5'-monophosphate oxidoreductase Short name=Guanosine monophosphate reductase | ||||
| Gene names |
| ||||
| Organism | Escherichia coli (strain K12) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 83333 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 347 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides. |
| Catalytic activity | Inosine 5'-phosphate + NH(3) + NADP(+) = guanosine 5'-phosphate + NADPH. |
| Subunit structure | Homotetramer. |
| Sequence similarities | Belongs to the IMPDH/GMPR family. GuaC type 1 subfamily. |
Ontologies
Keywords | |
|---|---|
| Ligand | Metal-binding NADP Potassium |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Direct protein sequencing |
Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide metabolic processInferred from electronic annotation. Source: HAMAP |
| Molecular function | GMP reductase activity Inferred from electronic annotation. Source: HAMAP potassium ion bindingInferred from electronic annotation. Source: UniProtKB-KW protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| dhaK | P76015 | 1 | EBI-544491,EBI-544485 | |
| pepP | P15034 | 2 | EBI-544491,EBI-554801 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 347 | 347 | GMP reductase | PRO_0000093735 | |||||
Regions | |||||||||
| Nucleotide binding | 108 – 131 | 24 | NADP By similarity | ||||||
| Nucleotide binding | 216 – 239 | 24 | NADP By similarity | ||||||
Sites | |||||||||
| Active site | 186 | 1 | Thioimidate intermediate By similarity | ||||||
| Metal binding | 181 | 1 | Potassium; via carbonyl oxygen By similarity | ||||||
| Metal binding | 183 | 1 | Potassium; via carbonyl oxygen By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 233 – 235 | 3 | GGG → AR Ref.1 Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Nucleotide sequence of the gene encoding the GMP reductase of Escherichia coli K12." Andrews S.C., Guest J.R. Biochem. J. 255:35-43(1988) [PubMed: 2904262] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12. |
| [2] | "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Fujita N., Mori H., Yura T., Ishihama A. Nucleic Acids Res. 22:1637-1639(1994) [PubMed: 8202364] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [3] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [4] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 233-235. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [5] | "Escherichia coli contains a set of genes homologous to those involved in protein secretion, DNA uptake and the assembly of type-4 fimbriae in other bacteria." Whitchurch C.B., Mattick J.S. Gene 150:9-15(1994) [PubMed: 7959070] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 244-347. Strain: K12. |
| [6] | "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-12. Strain: K12 / EMG2. |
Cross-references
Sequence databases | |
|---|---|
| X07917 Genomic DNA. Translation: CAA30751.1. U00096 Genomic DNA. Translation: AAC73215.1. AP009048 Genomic DNA. Translation: BAB96673.2. L28105 Genomic DNA. Translation: AAC36926.1. | |
| PIR | H64732. |
| RefSeq | AP_000766.1. NP_414646.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1B3O based on UniProtKB P12268. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P60560. |
2-D gel databases | |
| ECO2DBASE | H037.4. 6TH EDITION. |
Genome annotation databases | |
| GeneID | 948986. |
| GenomeReviews | Gene locus b0104 in contig U00096_GR. Gene locus JW0101 in contig AP009048_GR. |
| KEGG | ecj:JW0101. eco:b0104. |
Organism-specific databases | |
| EchoBASE | EB0417. |
| EcoGene | EG10422. guaC. |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P60560. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:GMP-REDUCT-MON. MetaCyc:GMP-REDUCT-MON. |
Family and domain databases | |
| HAMAP | MF_00596. [Tree] |
| InterPro | IPR013785. Aldolase_TIM. IPR005993. GMP_reduct1. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DHase_GMPRtase. [Graphical view] |
| Gene3D | G3DSA:3.20.20.70. Aldolase_TIM. 1 hit. |
| Pfam | PF00478. IMPDH. 1 hit. [Graphical view] |
| PIRSF | PIRSF000235. GMP_reductase. 1 hit. |
| TIGRFAMs | TIGR01305. GMP_reduct_1. 1 hit. |
| PROSITE | PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | GUAC_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P60560 Secondary accession number(s): P15344, P78048 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| SIMILARITY comments Index of protein domains and families |

Clusters with


