guaC

Functionⁱ

Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides.

Catalytic activityⁱ

Inosine 5'-phosphate + NH₃ + NADP⁺ = guanosine 5'-phosphate + NADPH.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	181	Potassium; via carbonyl oxygenBy similarity	1
Metal bindingⁱ	183	Potassium; via carbonyl oxygenBy similarity	1
Active siteⁱ	186	Thioimidate intermediateBy similarity	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	108 – 131	NADPBy similarityAdd BLAST		24
Nucleotide bindingⁱ	216 – 239	NADPBy similarityAdd BLAST		24

GO - Molecular functionⁱ

GMP reductase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 21298178
identical protein binding
Source: EcoCyc
Inferred from direct assayⁱ
- 21298178
metal ion binding Source: UniProtKB-KW

Complete GO annotation...

GO - Biological processⁱ

purine ribonucleotide interconversion
Source: EcoCyc
Inferred from direct assayⁱ
- 14419794

Complete GO annotation...

Keywordsⁱ

Molecular function	Oxidoreductase
Ligand	Metal-binding, NADP, Potassium

Enzyme and pathway databases

BioCycⁱ	EcoCyc:GMP-REDUCT-MONOMER. MetaCyc:GMP-REDUCT-MONOMER.

BioCycⁱ

EcoCyc:GMP-REDUCT-MONOMER.
MetaCyc:GMP-REDUCT-MONOMER.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: GMP reductase (EC:1.7.1.7) Alternative name(s): Guanosine 5'-monophosphate oxidoreductase Short name: Guanosine monophosphate reductase
Gene namesⁱ	Name:guaC Ordered Locus Names:b0104, JW0101
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10422. guaC.

EcoGeneⁱ

EG10422. guaC.

Subcellular locationⁱ

GO - Cellular componentⁱ

cytosol
Source: EcoCyc
Inferred from direct assayⁱ
- 15911532
- 18304323
GMP reductase complex Source: UniProtKB-EC

Complete GO annotation...

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000093735	1 – 347	GMP reductaseAdd BLAST		347

Proteomic databases

PaxDbⁱ	P60560.
PRIDEⁱ	P60560.

Interactionⁱ

Subunit structureⁱ

Homotetramer.

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
pepP	P15034	2	EBI-544491,EBI-554801

With

Entry

#Exp.

IntAct

Notes

pepP

P15034

2

EBI-544491,EBI-554801

GO - Molecular functionⁱ

identical protein binding
Source: EcoCyc
Inferred from direct assayⁱ
- 21298178

Complete GO annotation...

Protein-protein interaction databases

BioGridⁱ	4261108. 10 interactors.
Database of interacting proteins More...DIPⁱ	DIP-47861N.
IntActⁱ	P60560. 6 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-1311378.
STRINGⁱ	316385.ECDH10B_0085.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P60560.
SMRⁱ	P60560.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the IMPDH/GMPR family. GuaC type 1 subfamily.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4105CP4. Bacteria. COG0516. LUCA.
HOGENOMⁱ	HOG000165756.
InParanoidⁱ	P60560.
KEGG Orthology (KO) More...KOⁱ	K00364.
PhylomeDBⁱ	P60560.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00381. IMPDH. 1 hit.
Gene3Dⁱ	3.20.20.70. 1 hit.
HAMAPⁱ	MF_00596. GMP_reduct_type1. 1 hit.
InterProⁱ	View protein in InterPro IPR013785. Aldolase_TIM. IPR005993. GMPR. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00478. IMPDH. 1 hit.
PIRSFⁱ	PIRSF000235. GMP_reductase. 1 hit.
TIGRFAMsⁱ	TIGR01305. GMP_reduct_1. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00487. IMP_DH_GMP_RED. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

P60560-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MRIEEDLKLG FKDVLIRPKR STLKSRSDVE LERQFTFKHS GQSWSGVPII 
        60         70         80         90        100
AANMDTVGTF SMASALASFD ILTAVHKHYS VEEWQAFINN SSADVLKHVM 
       110        120        130        140        150
VSTGTSDADF EKTKQILDLN PALNFVCIDV ANGYSEHFVQ FVAKAREAWP 
       160        170        180        190        200
TKTICAGNVV TGEMCEELIL SGADIVKVGI GPGSVCTTRV KTGVGYPQLS 
       210        220        230        240        250
AVIECADAAH GLGGMIVSDG GCTTPGDVAK AFGGGADFVM LGGMLAGHEE 
       260        270        280        290        300
SGGRIVEENG EKFMLFYGMS SESAMKRHVG GVAEYRAAEG KTVKLPLRGP 
       310        320        330        340 
VENTARDILG GLRSACTYVG ASRLKELTKR TTFIRVQEQE NRIFNNL

Length:347

Mass (Da):37,384

Last modified:March 15, 2004 - v1

Checksum:ⁱ898F50DA7FD00441

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	233 – 235	GGG → AR (PubMed:2904262).Curated		3
Sequence conflictⁱ	233 – 235	GGG → AR (PubMed:8202364).Curated		3

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X07917 Genomic DNA. Translation: CAA30751.1. U00096 Genomic DNA. Translation: AAC73215.1. AP009048 Genomic DNA. Translation: BAB96673.2. L28105 Genomic DNA. Translation: AAC36926.1.
PIRⁱ	H64732.
NCBI Reference Sequences More...RefSeqⁱ	NP_414646.1. NC_000913.3. WP_001217338.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaⁱ	AAC73215; AAC73215; b0104. BAB96673; BAB96673; BAB96673.
GeneIDⁱ	948986.
KEGGⁱ	ecj:JW0101. eco:b0104.
PATRICⁱ	fig\|1411691.4.peg.2177.

Similar proteinsⁱ

Entry	Cluster members	Organisms	Length	Cluster ID	Cluster name	Size
P60560	B7UIF3 B7MAM6 B7LFX2 B7M145 C4ZRJ8 B1XC80 A7ZW55 A1A7E7 Q0TLN6 P60561 +111	Escherichia coli O127:H6 (strain E2348/69 / EPEC) Escherichia coli O45:K1 (strain S88 / ExPEC) Escherichia coli (strain 55989 / EAEC) Escherichia coli O8 (strain IAI1) Escherichia coli (strain K12 / MC4100 / BW2952) Escherichia coli (strain K12 / DH10B) Escherichia coli O9:H4 (strain HS) Escherichia coli O1:K1 / APEC Escherichia coli O6:K15:H31 (strain 536 / UPEC) Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) And more	347	UniRef100_B7UIF3	Cluster: GMP reductase	122

Entry	Cluster members	Organisms	Length	Cluster ID	Cluster name	Size
P60560	A9MQA3 A0A1X2TQJ9 A0A0V2EJE5 A0A1M3YA33 S3J4L4 UPI0003A105BB UPI000379EEA9 C0Q5K1 Q8Z9F9 UPI0005B64C89 +516	Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980) Salmonella enterica subsp. arizonae serovar 41:z4,z23:- Salmonella enterica subsp. arizonae serovar 63:g,z51:- str. So 20/20 Salmonella enterica subsp. arizonae serovar 18:z4,z23:- str. CVM N6509 Cedecea davisae DSM 4568 Cedecea davisae Escherichia coli Salmonella paratyphi C (strain RKS4594) Salmonella typhi Salmonella choleraesuis And more	347	UniRef90_A9MQA3	Cluster: GMP reductase	527

Entry	Cluster members	Organisms	Length	Cluster ID	Cluster name	Size
P60560	P59075 D0NS97 H3G8N5 A0A0W8C6L7 W2HJI0 W2QQ29 A0A081AXD8 W2XQ45 W2ZZ57 W2JNN7 +974	Phytophthora infestans (Potato late blight fungus) (Botrytis infestans) Phytophthora infestans (strain T30-4) (Potato late blight fungus) Phytophthora ramorum (Sudden oak death agent) Phytophthora nicotianae (Buckeye rot agent) Phytophthora parasitica (Potato buckeye rot agent) Phytophthora parasitica (strain INRA-310) Phytophthora parasitica P1976 Phytophthora parasitica CJ01A1 Phytophthora parasitica P10297 Phytophthora parasitica P1569 And more	362	UniRef50_P59075	Cluster: GMP reductase	985

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X07917 Genomic DNA. Translation: CAA30751.1. U00096 Genomic DNA. Translation: AAC73215.1. AP009048 Genomic DNA. Translation: BAB96673.2. L28105 Genomic DNA. Translation: AAC36926.1.
PIRⁱ	H64732.
NCBI Reference Sequences More...RefSeqⁱ	NP_414646.1. NC_000913.3. WP_001217338.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortalⁱ	P60560.
SMRⁱ	P60560.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4261108. 10 interactors.
Database of interacting proteins More...DIPⁱ	DIP-47861N.
IntActⁱ	P60560. 6 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-1311378.
STRINGⁱ	316385.ECDH10B_0085.

Proteomic databases

PaxDbⁱ	P60560.
PRIDEⁱ	P60560.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC73215; AAC73215; b0104. BAB96673; BAB96673; BAB96673.
GeneIDⁱ	948986.
KEGGⁱ	ecj:JW0101. eco:b0104.
PATRICⁱ	fig\|1411691.4.peg.2177.

Organism-specific databases

EchoBASEⁱ	EB0417.
EcoGeneⁱ	EG10422. guaC.

Phylogenomic databases

eggNOGⁱ	ENOG4105CP4. Bacteria. COG0516. LUCA.
HOGENOMⁱ	HOG000165756.
InParanoidⁱ	P60560.
KEGG Orthology (KO) More...KOⁱ	K00364.
PhylomeDBⁱ	P60560.

Enzyme and pathway databases

BioCycⁱ	EcoCyc:GMP-REDUCT-MONOMER. MetaCyc:GMP-REDUCT-MONOMER.

BioCycⁱ

EcoCyc:GMP-REDUCT-MONOMER.
MetaCyc:GMP-REDUCT-MONOMER.

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P60560.

PROⁱ

PR:P60560.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00381. IMPDH. 1 hit.
Gene3Dⁱ	3.20.20.70. 1 hit.
HAMAPⁱ	MF_00596. GMP_reduct_type1. 1 hit.
InterProⁱ	View protein in InterPro IPR013785. Aldolase_TIM. IPR005993. GMPR. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00478. IMPDH. 1 hit.
PIRSFⁱ	PIRSF000235. GMP_reductase. 1 hit.
TIGRFAMsⁱ	TIGR01305. GMP_reduct_1. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00487. IMP_DH_GMP_RED. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	GUAC_ECOLI
Accessionⁱ	P60560Primary (citable) accession number: P60560 Secondary accession number(s): P15344, P78048
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
	Last sequence update:	March 15, 2004
	Last modified:	August 30, 2017
	This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P60560 (GUAC_ECOLI)

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GMP reductase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ