Guanylate kinase - Escherichia coli (strain K12)

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P60546 (KGUA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 72. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Guanylate kinase
EC=2.7.4.8

Alternative name(s):
GMP kinase

Gene names

Name:	gmk
Synonyms:	spoR
Ordered Locus Names:	b3648, JW3623

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for recycling GMP and indirectly, cGMP. Ref.1
Catalytic activity	ATP + GMP = ADP + GDP. HAMAP MF_00328
Subunit structure	Homotetramer (under low ionic conditions) or homodimer (under high ionic conditions). Ref.1
Subcellular location	Cytoplasm HAMAP MF_00328.
Sequence similarities	Belongs to the guanylate kinase family. Contains 1 guanylate kinase-like domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	purine nucleotide metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW guanylate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 207

207

Guanylate kinase HAMAP MF_00328

PRO_0000170534

Regions

Domain

4 – 184

181

Guanylate kinase-like

Nucleotide binding

11 – 18

ATP By similarity

Secondary structure

207

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P60546 [UniParc].

Last modified March 15, 2004. Version 1.
Checksum: 62A99DB4063651E4
Show »

FASTA

207

23,593

        10         20         30         40         50         60 
MAQGTLYIVS APSGAGKSSL IQALLKTQPL YDTQVSVSHT TRQPRPGEVH GEHYFFVNHD 

        70         80         90        100        110        120 
EFKEMISRDA FLEHAEVFGN YYGTSREAIE QVLATGVDVF LDIDWQGAQQ IRQKMPHARS 

       130        140        150        160        170        180 
IFILPPSKIE LDRRLRGRGQ DSEEVIAKRM AQAVAEMSHY AEYDYLIVND DFDTALTDLK 

       190        200 
TIIRAERLRM SRQKQRHDAL ISKLLAD

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Guanylate kinase of Escherichia coli K-12." Gentry D., Bengra C., Ikehara K., Cashel M. J. Biol. Chem. 268:14316-14321(1993) [PubMed: 8390989] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT. Strain: K12 / JM109 / ATCC 53323.
[2]	"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R. Genomics 16:551-561(1993) [PubMed: 7686882] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M84400 Genomic DNA. Translation: AAB88711.1.
L10328 Genomic DNA. Translation: AAA62001.1.
U00096 Genomic DNA. Translation: AAC76672.1.
AP009048 Genomic DNA. Translation: BAE77645.1.

PIR

KIECGU. S43041.

RefSeq

NP_418105.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1S96	X-ray	2.00	A/B	1-207	[»]
2AN9	X-ray	2.35	A/B	1-207	[»]
2ANB	X-ray	2.90	A	1-207	[»]
2ANC	X-ray	3.20	A/B/C/D/E/F	1-207	[»]
2F3R	X-ray	2.50	A/B	1-207	[»]
2F3T	X-ray	3.16	A/B/C/D/E/F	1-207	[»]

ProteinModelPortal

P60546.

SMR

P60546. Positions 3-207.

ModBase

Search...

Protein-protein interaction databases

IntAct

P60546. 5 interactions.

MINT

MINT-1286577.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000004277; EBESCP00000004277; EBESCG00000003488.
EBESCT00000016920; EBESCP00000016211; EBESCG00000015979.

GeneID

948163.

GenomeReviews

Gene locus JW3623 in contig AP009048_GR.
Gene locus b3648 in contig U00096_GR.

KEGG

ecj:JW3623.
eco:b3648.

PATRIC

32122783. VBIEscCol129921_3768.

Organism-specific databases

EchoBASE

EB0958.

EcoGene

EG10965. gmk.

Phylogenomic databases

eggNOG

COG0194.

GeneTree

EBGT00050000010982.

HOGENOM

HBG671982.

OMA

DIDWQGT.

PhylomeDB

P60546.

ProtClustDB

PRK00300.

Enzyme and pathway databases

BioCyc

EcoCyc:GUANYL-KIN-MONOMER.
MetaCyc:GUANYL-KIN-MONOMER.

Gene expression databases

Genevestigator

P60546.

Family and domain databases

HAMAP

MF_00328. Guanylate_kinase.
[Tree]

InterPro

IPR008144. Guanylate_kin.
IPR008145. Guanylate_kin/L-typ_Ca_channel.
IPR020590. Guanylate_kinase_CS.
IPR017665. Guanylate_kinase_sub.
[Graphical view]

K00942.

Pfam

PF00625. Guanylate_kin. 1 hit.
[Graphical view]

SMART

SM00072. GuKc. 1 hit.
[Graphical view]

TIGRFAMs

TIGR03263. Guanyl_kin. 1 hit.

PROSITE

PS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

KGUA_ECOLI

Accession

Primary (citable) accession number: P60546
Secondary accession number(s): P24234, Q2M7W1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	March 15, 2004
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents