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Protein

Guanylate kinase

Gene

gmk

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential for recycling GMP and indirectly, cGMP.1 Publication

Catalytic activityi

ATP + GMP = ADP + GDP.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 188ATPBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • guanylate kinase activity Source: EcoCyc
  • identical protein binding Source: EcoCyc

GO - Biological processi

  • nucleotide phosphorylation Source: GOC
  • purine nucleotide metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:GUANYL-KIN-MONOMER.
ECOL316407:JW3623-MONOMER.
MetaCyc:GUANYL-KIN-MONOMER.
BRENDAi2.7.4.8. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylate kinase (EC:2.7.4.8)
Alternative name(s):
GMP kinase
Gene namesi
Name:gmk
Synonyms:spoR
Ordered Locus Names:b3648, JW3623
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10965. gmk.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 207207Guanylate kinasePRO_0000170534Add
BLAST

Proteomic databases

PaxDbiP60546.
PRIDEiP60546.

Expressioni

Gene expression databases

GenevestigatoriP60546.

Interactioni

Subunit structurei

Homotetramer (under low ionic conditions) or homodimer (under high ionic conditions).1 Publication

Protein-protein interaction databases

IntActiP60546. 5 interactions.
MINTiMINT-1286577.
STRINGi511145.b3648.

Structurei

Secondary structure

1
207
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 105Combined sources
Beta strandi13 – 153Combined sources
Helixi17 – 2711Combined sources
Turni30 – 323Combined sources
Beta strandi33 – 353Combined sources
Beta strandi38 – 425Combined sources
Turni50 – 523Combined sources
Beta strandi53 – 564Combined sources
Helixi59 – 679Combined sources
Beta strandi71 – 777Combined sources
Beta strandi80 – 856Combined sources
Helixi86 – 938Combined sources
Turni94 – 963Combined sources
Beta strandi98 – 1025Combined sources
Helixi105 – 11410Combined sources
Beta strandi119 – 1246Combined sources
Helixi128 – 1369Combined sources
Beta strandi138 – 1414Combined sources
Helixi143 – 15715Combined sources
Helixi158 – 1625Combined sources
Beta strandi163 – 1686Combined sources
Helixi172 – 18716Combined sources
Helixi190 – 1967Combined sources
Helixi198 – 2058Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1S96X-ray2.00A/B1-207[»]
2AN9X-ray2.35A/B1-207[»]
2ANBX-ray2.90A1-207[»]
2ANCX-ray3.20A/B/C/D/E/F1-207[»]
2F3RX-ray2.50A/B1-207[»]
2F3TX-ray3.16A/B/C/D/E/F1-207[»]
ProteinModelPortaliP60546.
SMRiP60546. Positions 3-207.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60546.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 184181Guanylate kinase-likeAdd
BLAST

Sequence similaritiesi

Belongs to the guanylate kinase family.Curated
Contains 1 guanylate kinase-like domain.Curated

Phylogenomic databases

eggNOGiCOG0194.
HOGENOMiHOG000037638.
InParanoidiP60546.
KOiK00942.
OMAiMSHYNEY.
OrthoDBiEOG6CP410.
PhylomeDBiP60546.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
HAMAPiMF_00328. Guanylate_kinase.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like.
IPR017665. Guanylate_kinase.
IPR020590. Guanylate_kinase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00625. Guanylate_kin. 1 hit.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR03263. guanyl_kin. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P60546-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQGTLYIVS APSGAGKSSL IQALLKTQPL YDTQVSVSHT TRQPRPGEVH
60 70 80 90 100
GEHYFFVNHD EFKEMISRDA FLEHAEVFGN YYGTSREAIE QVLATGVDVF
110 120 130 140 150
LDIDWQGAQQ IRQKMPHARS IFILPPSKIE LDRRLRGRGQ DSEEVIAKRM
160 170 180 190 200
AQAVAEMSHY AEYDYLIVND DFDTALTDLK TIIRAERLRM SRQKQRHDAL

ISKLLAD
Length:207
Mass (Da):23,593
Last modified:March 15, 2004 - v1
Checksum:i62A99DB4063651E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84400 Genomic DNA. Translation: AAB88711.1.
L10328 Genomic DNA. Translation: AAA62001.1.
U00096 Genomic DNA. Translation: AAC76672.1.
AP009048 Genomic DNA. Translation: BAE77645.1.
PIRiS43041. KIECGU.
RefSeqiNP_418105.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC76672; AAC76672; b3648.
BAE77645; BAE77645; BAE77645.
GeneIDi948163.
KEGGiecj:Y75_p3526.
eco:b3648.
PATRICi32122783. VBIEscCol129921_3768.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84400 Genomic DNA. Translation: AAB88711.1.
L10328 Genomic DNA. Translation: AAA62001.1.
U00096 Genomic DNA. Translation: AAC76672.1.
AP009048 Genomic DNA. Translation: BAE77645.1.
PIRiS43041. KIECGU.
RefSeqiNP_418105.1. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1S96X-ray2.00A/B1-207[»]
2AN9X-ray2.35A/B1-207[»]
2ANBX-ray2.90A1-207[»]
2ANCX-ray3.20A/B/C/D/E/F1-207[»]
2F3RX-ray2.50A/B1-207[»]
2F3TX-ray3.16A/B/C/D/E/F1-207[»]
ProteinModelPortaliP60546.
SMRiP60546. Positions 3-207.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP60546. 5 interactions.
MINTiMINT-1286577.
STRINGi511145.b3648.

Proteomic databases

PaxDbiP60546.
PRIDEiP60546.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76672; AAC76672; b3648.
BAE77645; BAE77645; BAE77645.
GeneIDi948163.
KEGGiecj:Y75_p3526.
eco:b3648.
PATRICi32122783. VBIEscCol129921_3768.

Organism-specific databases

EchoBASEiEB0958.
EcoGeneiEG10965. gmk.

Phylogenomic databases

eggNOGiCOG0194.
HOGENOMiHOG000037638.
InParanoidiP60546.
KOiK00942.
OMAiMSHYNEY.
OrthoDBiEOG6CP410.
PhylomeDBiP60546.

Enzyme and pathway databases

BioCyciEcoCyc:GUANYL-KIN-MONOMER.
ECOL316407:JW3623-MONOMER.
MetaCyc:GUANYL-KIN-MONOMER.
BRENDAi2.7.4.8. 2026.

Miscellaneous databases

EvolutionaryTraceiP60546.
PROiP60546.

Gene expression databases

GenevestigatoriP60546.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
HAMAPiMF_00328. Guanylate_kinase.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like.
IPR017665. Guanylate_kinase.
IPR020590. Guanylate_kinase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00625. Guanylate_kin. 1 hit.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR03263. guanyl_kin. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Guanylate kinase of Escherichia coli K-12."
    Gentry D., Bengra C., Ikehara K., Cashel M.
    J. Biol. Chem. 268:14316-14321(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT.
    Strain: K12 / JM109 / ATCC 53323.
  2. "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
    Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
    Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiKGUA_ECOLI
AccessioniPrimary (citable) accession number: P60546
Secondary accession number(s): P24234, Q2M7W1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: March 15, 2004
Last modified: May 27, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.