P60546 (KGUA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanylate kinase
EC=2.7.4.8

Alternative name(s):
GMP kinase

Gene names

Name:	gmk
Synonyms:	spoR
Ordered Locus Names:	b3648, JW3623

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for recycling GMP and indirectly, cGMP. Ref.1
Catalytic activity	ATP + GMP = ADP + GDP. HAMAP-Rule MF_00328
Subunit structure	Homotetramer (under low ionic conditions) or homodimer (under high ionic conditions). Ref.1
Subcellular location	Cytoplasm HAMAP-Rule MF_00328.
Sequence similarities	Belongs to the guanylate kinase family. Contains 1 guanylate kinase-like domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	purine nucleotide metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW guanylate kinase activity Inferred from direct assay Ref.1. Source: EcoCyc identical protein binding Inferred from direct assay PubMed 16140325Ref.1. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 207

207

Guanylate kinase HAMAP-Rule MF_00328

PRO_0000170534

Regions

Domain

4 – 184

181

Guanylate kinase-like

Nucleotide binding

11 – 18

8

ATP By similarity

Secondary structure

1

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207

Helix Strand Turn

Details...

Beta strand

6 – 10

5

Beta strand

13 – 15

3

Helix

17 – 27

11

Turn

30 – 32

3

Beta strand

33 – 35

3

Beta strand

38 – 42

5

Turn

50 – 52

3

Beta strand

53 – 56

4

Helix

59 – 67

9

Beta strand

71 – 77

7

Beta strand

80 – 85

6

Helix

86 – 93

8

Turn

94 – 96

3

Beta strand

98 – 102

5

Helix

105 – 114

10

Beta strand

119 – 124

6

Helix

128 – 136

9

Beta strand

138 – 141

4

Helix

143 – 157

15

Helix

158 – 162

5

Beta strand

163 – 168

6

Helix

172 – 187

16

Helix

190 – 196

7

Helix

198 – 205

8

Sequences

	Sequence		Length	Mass (Da)
	P60546 [UniParc]. Last modified March 15, 2004. Version 1. Checksum: 62A99DB4063651E4	FASTA	207	23,593
	10 20 30 40 50 60 MAQGTLYIVS APSGAGKSSL IQALLKTQPL YDTQVSVSHT TRQPRPGEVH GEHYFFVNHD 70 80 90 100 110 120 EFKEMISRDA FLEHAEVFGN YYGTSREAIE QVLATGVDVF LDIDWQGAQQ IRQKMPHARS 130 140 150 160 170 180 IFILPPSKIE LDRRLRGRGQ DSEEVIAKRM AQAVAEMSHY AEYDYLIVND DFDTALTDLK 190 200 TIIRAERLRM SRQKQRHDAL ISKLLAD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Guanylate kinase of Escherichia coli K-12." Gentry D., Bengra C., Ikehara K., Cashel M. J. Biol. Chem. 268:14316-14321(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT. Strain: K12 / JM109 / ATCC 53323.
[2]	"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R. Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M84400 Genomic DNA. Translation: AAB88711.1.
L10328 Genomic DNA. Translation: AAA62001.1.
U00096 Genomic DNA. Translation: AAC76672.1.
AP009048 Genomic DNA. Translation: BAE77645.1.

PIR

KIECGU. S43041.

RefSeq

NP_418105.1. NC_000913.2.
YP_491786.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1S96	X-ray	2.00	A/B	1-207	[»]
2AN9	X-ray	2.35	A/B	1-207	[»]
2ANB	X-ray	2.90	A	1-207	[»]
2ANC	X-ray	3.20	A/B/C/D/E/F	1-207	[»]
2F3R	X-ray	2.50	A/B	1-207	[»]
2F3T	X-ray	3.16	A/B/C/D/E/F	1-207	[»]

ProteinModelPortal

P60546.

SMR

P60546. Positions 3-207.

ModBase

Search...

Protein-protein interaction databases

IntAct

P60546. 5 interactions.

MINT

MINT-1286577.

STRING

511145.b3648.

Proteomic databases

PaxDb

P60546.

PRIDE

P60546.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC76672; AAC76672; b3648.
BAE77645; BAE77645; BAE77645.

GeneID

12933574.
948163.

KEGG

ecj:Y75_p3526.
eco:b3648.

PATRIC

32122783. VBIEscCol129921_3768.

Organism-specific databases

EchoBASE

EB0958.

EcoGene

EG10965. gmk.

Phylogenomic databases

eggNOG

COG0194.

HOGENOM

HOG000037638.

KO

K00942.

OMA

FVIINEL.

ProtClustDB

PRK00300.

Enzyme and pathway databases

BioCyc

EcoCyc:GUANYL-KIN-MONOMER.
ECOL316407:JW3623-MONOMER.
MetaCyc:GUANYL-KIN-MONOMER.

Gene expression databases

Genevestigator

P60546.

Family and domain databases

HAMAP

MF_00328. Guanylate_kinase.

InterPro

IPR008144. Guanylate_kin.
IPR008145. Guanylate_kin/L-typ_Ca_channel.
IPR020590. Guanylate_kinase_CS.
IPR017665. Guanylate_kinase_sub.
IPR027417. P-loop_NTPase.
[Graphical view]

Pfam

PF00625. Guanylate_kin. 1 hit.
[Graphical view]

SMART

SM00072. GuKc. 1 hit.
[Graphical view]

SUPFAM

SSF52540. SSF52540. 1 hit.

TIGRFAMs

TIGR03263. guanyl_kin. 1 hit.

PROSITE

PS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P60546.

Entry information

Entry name

KGUA_ECOLI

Accession

Primary (citable) accession number: P60546
Secondary accession number(s): P24234, Q2M7W1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	March 15, 2004
Last modified:	May 29, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program