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Protein

Guanylate kinase

Gene

gmk

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential for recycling GMP and indirectly, cGMP.1 Publication

Catalytic activityi

ATP + GMP = ADP + GDP.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 18ATPBy similarity8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • guanylate kinase activity Source: EcoCyc
  • identical protein binding Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:GUANYL-KIN-MONOMER.
ECOL316407:JW3623-MONOMER.
MetaCyc:GUANYL-KIN-MONOMER.
BRENDAi2.7.4.8. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylate kinase (EC:2.7.4.8)
Alternative name(s):
GMP kinase
Gene namesi
Name:gmk
Synonyms:spoR
Ordered Locus Names:b3648, JW3623
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10965. gmk.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001705341 – 207Guanylate kinaseAdd BLAST207

Proteomic databases

EPDiP60546.
PaxDbiP60546.
PRIDEiP60546.

Interactioni

Subunit structurei

Homotetramer (under low ionic conditions) or homodimer (under high ionic conditions).1 Publication

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4262568. 3 interactors.
IntActiP60546. 5 interactors.
MINTiMINT-1286577.
STRINGi511145.b3648.

Structurei

Secondary structure

1207
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 10Combined sources5
Beta strandi13 – 15Combined sources3
Helixi17 – 27Combined sources11
Turni30 – 32Combined sources3
Beta strandi33 – 35Combined sources3
Beta strandi38 – 42Combined sources5
Turni50 – 52Combined sources3
Beta strandi53 – 56Combined sources4
Helixi59 – 67Combined sources9
Beta strandi71 – 77Combined sources7
Beta strandi80 – 85Combined sources6
Helixi86 – 93Combined sources8
Turni94 – 96Combined sources3
Beta strandi98 – 102Combined sources5
Helixi105 – 114Combined sources10
Beta strandi119 – 124Combined sources6
Helixi128 – 136Combined sources9
Beta strandi138 – 141Combined sources4
Helixi143 – 157Combined sources15
Helixi158 – 162Combined sources5
Beta strandi163 – 168Combined sources6
Helixi172 – 187Combined sources16
Helixi190 – 196Combined sources7
Helixi198 – 205Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S96X-ray2.00A/B1-207[»]
2AN9X-ray2.35A/B1-207[»]
2ANBX-ray2.90A1-207[»]
2ANCX-ray3.20A/B/C/D/E/F1-207[»]
2F3RX-ray2.50A/B1-207[»]
2F3TX-ray3.16A/B/C/D/E/F1-207[»]
ProteinModelPortaliP60546.
SMRiP60546.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60546.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 184Guanylate kinase-likeAdd BLAST181

Sequence similaritiesi

Belongs to the guanylate kinase family.Curated
Contains 1 guanylate kinase-like domain.Curated

Phylogenomic databases

eggNOGiENOG4108UHA. Bacteria.
COG0194. LUCA.
HOGENOMiHOG000037638.
InParanoidiP60546.
KOiK00942.
OMAiDYCVIND.
PhylomeDBiP60546.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
HAMAPiMF_00328. Guanylate_kinase. 1 hit.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR017665. Guanylate_kinase.
IPR020590. Guanylate_kinase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00625. Guanylate_kin. 1 hit.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR03263. guanyl_kin. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P60546-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQGTLYIVS APSGAGKSSL IQALLKTQPL YDTQVSVSHT TRQPRPGEVH
60 70 80 90 100
GEHYFFVNHD EFKEMISRDA FLEHAEVFGN YYGTSREAIE QVLATGVDVF
110 120 130 140 150
LDIDWQGAQQ IRQKMPHARS IFILPPSKIE LDRRLRGRGQ DSEEVIAKRM
160 170 180 190 200
AQAVAEMSHY AEYDYLIVND DFDTALTDLK TIIRAERLRM SRQKQRHDAL

ISKLLAD
Length:207
Mass (Da):23,593
Last modified:March 15, 2004 - v1
Checksum:i62A99DB4063651E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84400 Genomic DNA. Translation: AAB88711.1.
L10328 Genomic DNA. Translation: AAA62001.1.
U00096 Genomic DNA. Translation: AAC76672.1.
AP009048 Genomic DNA. Translation: BAE77645.1.
PIRiS43041. KIECGU.
RefSeqiNP_418105.1. NC_000913.3.
WP_001295237.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76672; AAC76672; b3648.
BAE77645; BAE77645; BAE77645.
GeneIDi948163.
KEGGiecj:JW3623.
eco:b3648.
PATRICi32122783. VBIEscCol129921_3768.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84400 Genomic DNA. Translation: AAB88711.1.
L10328 Genomic DNA. Translation: AAA62001.1.
U00096 Genomic DNA. Translation: AAC76672.1.
AP009048 Genomic DNA. Translation: BAE77645.1.
PIRiS43041. KIECGU.
RefSeqiNP_418105.1. NC_000913.3.
WP_001295237.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S96X-ray2.00A/B1-207[»]
2AN9X-ray2.35A/B1-207[»]
2ANBX-ray2.90A1-207[»]
2ANCX-ray3.20A/B/C/D/E/F1-207[»]
2F3RX-ray2.50A/B1-207[»]
2F3TX-ray3.16A/B/C/D/E/F1-207[»]
ProteinModelPortaliP60546.
SMRiP60546.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262568. 3 interactors.
IntActiP60546. 5 interactors.
MINTiMINT-1286577.
STRINGi511145.b3648.

Proteomic databases

EPDiP60546.
PaxDbiP60546.
PRIDEiP60546.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76672; AAC76672; b3648.
BAE77645; BAE77645; BAE77645.
GeneIDi948163.
KEGGiecj:JW3623.
eco:b3648.
PATRICi32122783. VBIEscCol129921_3768.

Organism-specific databases

EchoBASEiEB0958.
EcoGeneiEG10965. gmk.

Phylogenomic databases

eggNOGiENOG4108UHA. Bacteria.
COG0194. LUCA.
HOGENOMiHOG000037638.
InParanoidiP60546.
KOiK00942.
OMAiDYCVIND.
PhylomeDBiP60546.

Enzyme and pathway databases

BioCyciEcoCyc:GUANYL-KIN-MONOMER.
ECOL316407:JW3623-MONOMER.
MetaCyc:GUANYL-KIN-MONOMER.
BRENDAi2.7.4.8. 2026.

Miscellaneous databases

EvolutionaryTraceiP60546.
PROiP60546.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
HAMAPiMF_00328. Guanylate_kinase. 1 hit.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR017665. Guanylate_kinase.
IPR020590. Guanylate_kinase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00625. Guanylate_kin. 1 hit.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR03263. guanyl_kin. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKGUA_ECOLI
AccessioniPrimary (citable) accession number: P60546
Secondary accession number(s): P24234, Q2M7W1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: March 15, 2004
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.