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P60546 (KGUA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanylate kinase

EC=2.7.4.8
Alternative name(s):
GMP kinase
Gene names
Name:gmk
Synonyms:spoR
Ordered Locus Names:b3648, JW3623
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length207 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential for recycling GMP and indirectly, cGMP. Ref.1

Catalytic activity

ATP + GMP = ADP + GDP. HAMAP-Rule MF_00328

Subunit structure

Homotetramer (under low ionic conditions) or homodimer (under high ionic conditions). Ref.1

Subcellular location

Cytoplasm HAMAP-Rule MF_00328.

Sequence similarities

Belongs to the guanylate kinase family.

Contains 1 guanylate kinase-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 207207Guanylate kinase HAMAP-Rule MF_00328
PRO_0000170534

Regions

Domain4 – 184181Guanylate kinase-like
Nucleotide binding11 – 188ATP By similarity

Secondary structure

........................................... 207
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P60546 [UniParc].

Last modified March 15, 2004. Version 1.
Checksum: 62A99DB4063651E4

FASTA20723,593
        10         20         30         40         50         60 
MAQGTLYIVS APSGAGKSSL IQALLKTQPL YDTQVSVSHT TRQPRPGEVH GEHYFFVNHD 

        70         80         90        100        110        120 
EFKEMISRDA FLEHAEVFGN YYGTSREAIE QVLATGVDVF LDIDWQGAQQ IRQKMPHARS 

       130        140        150        160        170        180 
IFILPPSKIE LDRRLRGRGQ DSEEVIAKRM AQAVAEMSHY AEYDYLIVND DFDTALTDLK 

       190        200 
TIIRAERLRM SRQKQRHDAL ISKLLAD 

« Hide

References

« Hide 'large scale' references
[1]"Guanylate kinase of Escherichia coli K-12."
Gentry D., Bengra C., Ikehara K., Cashel M.
J. Biol. Chem. 268:14316-14321(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT.
Strain: K12 / JM109 / ATCC 53323.
[2]"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M84400 Genomic DNA. Translation: AAB88711.1.
L10328 Genomic DNA. Translation: AAA62001.1.
U00096 Genomic DNA. Translation: AAC76672.1.
AP009048 Genomic DNA. Translation: BAE77645.1.
PIRKIECGU. S43041.
RefSeqNP_418105.1. NC_000913.3.
YP_491786.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1S96X-ray2.00A/B1-207[»]
2AN9X-ray2.35A/B1-207[»]
2ANBX-ray2.90A1-207[»]
2ANCX-ray3.20A/B/C/D/E/F1-207[»]
2F3RX-ray2.50A/B1-207[»]
2F3TX-ray3.16A/B/C/D/E/F1-207[»]
ProteinModelPortalP60546.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP60546. 5 interactions.
MINTMINT-1286577.
STRING511145.b3648.

Proteomic databases

PaxDbP60546.
PRIDEP60546.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76672; AAC76672; b3648.
BAE77645; BAE77645; BAE77645.
GeneID12933574.
948163.
KEGGecj:Y75_p3526.
eco:b3648.
PATRIC32122783. VBIEscCol129921_3768.

Organism-specific databases

EchoBASEEB0958.
EcoGeneEG10965. gmk.

Phylogenomic databases

eggNOGCOG0194.
HOGENOMHOG000037638.
KOK00942.
OMAMSHYNEY.
OrthoDBEOG6CP410.
PhylomeDBP60546.

Enzyme and pathway databases

BioCycEcoCyc:GUANYL-KIN-MONOMER.
ECOL316407:JW3623-MONOMER.
MetaCyc:GUANYL-KIN-MONOMER.

Gene expression databases

GenevestigatorP60546.

Family and domain databases

Gene3D3.40.50.300. 3 hits.
HAMAPMF_00328. Guanylate_kinase.
InterProIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like.
IPR017665. Guanylate_kinase.
IPR020590. Guanylate_kinase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00625. Guanylate_kin. 1 hit.
[Graphical view]
SMARTSM00072. GuKc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR03263. guanyl_kin. 1 hit.
PROSITEPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP60546.
PROP60546.

Entry information

Entry nameKGUA_ECOLI
AccessionPrimary (citable) accession number: P60546
Secondary accession number(s): P24234, Q2M7W1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: March 15, 2004
Last modified: June 11, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene