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P60546

- KGUA_ECOLI

UniProt

P60546 - KGUA_ECOLI

Protein

Guanylate kinase

Gene

gmk

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (15 Mar 2004)
      Previous versions | rss
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    Functioni

    Essential for recycling GMP and indirectly, cGMP.1 Publication

    Catalytic activityi

    ATP + GMP = ADP + GDP.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi11 – 188ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. guanylate kinase activity Source: EcoCyc
    3. identical protein binding Source: EcoCyc

    GO - Biological processi

    1. nucleotide phosphorylation Source: GOC
    2. purine nucleotide metabolic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:GUANYL-KIN-MONOMER.
    ECOL316407:JW3623-MONOMER.
    MetaCyc:GUANYL-KIN-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanylate kinase (EC:2.7.4.8)
    Alternative name(s):
    GMP kinase
    Gene namesi
    Name:gmk
    Synonyms:spoR
    Ordered Locus Names:b3648, JW3623
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10965. gmk.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 207207Guanylate kinasePRO_0000170534Add
    BLAST

    Proteomic databases

    PaxDbiP60546.
    PRIDEiP60546.

    Expressioni

    Gene expression databases

    GenevestigatoriP60546.

    Interactioni

    Subunit structurei

    Homotetramer (under low ionic conditions) or homodimer (under high ionic conditions).1 Publication

    Protein-protein interaction databases

    IntActiP60546. 5 interactions.
    MINTiMINT-1286577.
    STRINGi511145.b3648.

    Structurei

    Secondary structure

    1
    207
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi6 – 105
    Beta strandi13 – 153
    Helixi17 – 2711
    Turni30 – 323
    Beta strandi33 – 353
    Beta strandi38 – 425
    Turni50 – 523
    Beta strandi53 – 564
    Helixi59 – 679
    Beta strandi71 – 777
    Beta strandi80 – 856
    Helixi86 – 938
    Turni94 – 963
    Beta strandi98 – 1025
    Helixi105 – 11410
    Beta strandi119 – 1246
    Helixi128 – 1369
    Beta strandi138 – 1414
    Helixi143 – 15715
    Helixi158 – 1625
    Beta strandi163 – 1686
    Helixi172 – 18716
    Helixi190 – 1967
    Helixi198 – 2058

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1S96X-ray2.00A/B1-207[»]
    2AN9X-ray2.35A/B1-207[»]
    2ANBX-ray2.90A1-207[»]
    2ANCX-ray3.20A/B/C/D/E/F1-207[»]
    2F3RX-ray2.50A/B1-207[»]
    2F3TX-ray3.16A/B/C/D/E/F1-207[»]
    ProteinModelPortaliP60546.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP60546.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 184181Guanylate kinase-likeAdd
    BLAST

    Sequence similaritiesi

    Belongs to the guanylate kinase family.Curated
    Contains 1 guanylate kinase-like domain.Curated

    Phylogenomic databases

    eggNOGiCOG0194.
    HOGENOMiHOG000037638.
    KOiK00942.
    OMAiMSHYNEY.
    OrthoDBiEOG6CP410.
    PhylomeDBiP60546.

    Family and domain databases

    Gene3Di3.40.50.300. 3 hits.
    HAMAPiMF_00328. Guanylate_kinase.
    InterProiIPR008145. GK/Ca_channel_bsu.
    IPR008144. Guanylate_kin-like.
    IPR017665. Guanylate_kinase.
    IPR020590. Guanylate_kinase_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00625. Guanylate_kin. 1 hit.
    [Graphical view]
    SMARTiSM00072. GuKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR03263. guanyl_kin. 1 hit.
    PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
    PS50052. GUANYLATE_KINASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P60546-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQGTLYIVS APSGAGKSSL IQALLKTQPL YDTQVSVSHT TRQPRPGEVH    50
    GEHYFFVNHD EFKEMISRDA FLEHAEVFGN YYGTSREAIE QVLATGVDVF 100
    LDIDWQGAQQ IRQKMPHARS IFILPPSKIE LDRRLRGRGQ DSEEVIAKRM 150
    AQAVAEMSHY AEYDYLIVND DFDTALTDLK TIIRAERLRM SRQKQRHDAL 200
    ISKLLAD 207
    Length:207
    Mass (Da):23,593
    Last modified:March 15, 2004 - v1
    Checksum:i62A99DB4063651E4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84400 Genomic DNA. Translation: AAB88711.1.
    L10328 Genomic DNA. Translation: AAA62001.1.
    U00096 Genomic DNA. Translation: AAC76672.1.
    AP009048 Genomic DNA. Translation: BAE77645.1.
    PIRiS43041. KIECGU.
    RefSeqiNP_418105.1. NC_000913.3.
    YP_491786.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76672; AAC76672; b3648.
    BAE77645; BAE77645; BAE77645.
    GeneIDi12933574.
    948163.
    KEGGiecj:Y75_p3526.
    eco:b3648.
    PATRICi32122783. VBIEscCol129921_3768.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84400 Genomic DNA. Translation: AAB88711.1 .
    L10328 Genomic DNA. Translation: AAA62001.1 .
    U00096 Genomic DNA. Translation: AAC76672.1 .
    AP009048 Genomic DNA. Translation: BAE77645.1 .
    PIRi S43041. KIECGU.
    RefSeqi NP_418105.1. NC_000913.3.
    YP_491786.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1S96 X-ray 2.00 A/B 1-207 [» ]
    2AN9 X-ray 2.35 A/B 1-207 [» ]
    2ANB X-ray 2.90 A 1-207 [» ]
    2ANC X-ray 3.20 A/B/C/D/E/F 1-207 [» ]
    2F3R X-ray 2.50 A/B 1-207 [» ]
    2F3T X-ray 3.16 A/B/C/D/E/F 1-207 [» ]
    ProteinModelPortali P60546.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P60546. 5 interactions.
    MINTi MINT-1286577.
    STRINGi 511145.b3648.

    Proteomic databases

    PaxDbi P60546.
    PRIDEi P60546.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76672 ; AAC76672 ; b3648 .
    BAE77645 ; BAE77645 ; BAE77645 .
    GeneIDi 12933574.
    948163.
    KEGGi ecj:Y75_p3526.
    eco:b3648.
    PATRICi 32122783. VBIEscCol129921_3768.

    Organism-specific databases

    EchoBASEi EB0958.
    EcoGenei EG10965. gmk.

    Phylogenomic databases

    eggNOGi COG0194.
    HOGENOMi HOG000037638.
    KOi K00942.
    OMAi MSHYNEY.
    OrthoDBi EOG6CP410.
    PhylomeDBi P60546.

    Enzyme and pathway databases

    BioCyci EcoCyc:GUANYL-KIN-MONOMER.
    ECOL316407:JW3623-MONOMER.
    MetaCyc:GUANYL-KIN-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P60546.
    PROi P60546.

    Gene expression databases

    Genevestigatori P60546.

    Family and domain databases

    Gene3Di 3.40.50.300. 3 hits.
    HAMAPi MF_00328. Guanylate_kinase.
    InterProi IPR008145. GK/Ca_channel_bsu.
    IPR008144. Guanylate_kin-like.
    IPR017665. Guanylate_kinase.
    IPR020590. Guanylate_kinase_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00625. Guanylate_kin. 1 hit.
    [Graphical view ]
    SMARTi SM00072. GuKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR03263. guanyl_kin. 1 hit.
    PROSITEi PS00856. GUANYLATE_KINASE_1. 1 hit.
    PS50052. GUANYLATE_KINASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Guanylate kinase of Escherichia coli K-12."
      Gentry D., Bengra C., Ikehara K., Cashel M.
      J. Biol. Chem. 268:14316-14321(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT.
      Strain: K12 / JM109 / ATCC 53323.
    2. "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
      Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
      Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiKGUA_ECOLI
    AccessioniPrimary (citable) accession number: P60546
    Secondary accession number(s): P24234, Q2M7W1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2004
    Last sequence update: March 15, 2004
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3