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P60522

- GBRL2_RAT

UniProt

P60522 - GBRL2_RAT

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Protein
Gamma-aminobutyric acid receptor-associated protein-like 2
Gene
Gabarapl2, Gef2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Ubiquitin-like modifier involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1. Involved in autophagy. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei116 – 1172Cleavage; by ATG4B By similarity

GO - Molecular functioni

  1. GABA receptor binding Source: RefGenome
  2. microtubule binding Source: RefGenome
  3. phosphatidylethanolamine binding Source: RefGenome

GO - Biological processi

  1. CVT pathway Source: RefGenome
  2. autophagic vacuole assembly Source: RefGenome
  3. cellular response to nitrogen starvation Source: RefGenome
  4. late nucleophagy Source: RefGenome
  5. mitochondrion degradation Source: RefGenome
  6. piecemeal microautophagy of nucleus Source: RefGenome
  7. protein targeting to vacuole involved in autophagy Source: RefGenome
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor-associated protein-like 2
Alternative name(s):
GABA(A) receptor-associated protein-like 2
Ganglioside expression factor 2
Short name:
GEF-2
Golgi-associated ATPase enhancer of 16 kDa
Short name:
GATE-16
Gene namesi
Name:Gabarapl2
Synonyms:Gef2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 19, UP000002494: Chromosome 8

Organism-specific databases

RGDi620510. Gabarapl2.

Subcellular locationi

GO - Cellular componenti

  1. Golgi membrane Source: RefGenome
  2. autophagic vacuole Source: UniProtKB
  3. autophagic vacuole membrane Source: RefGenome
  4. cytoplasmic vesicle Source: UniProtKB-KW
  5. cytosol Source: RefGenome
  6. pre-autophagosomal structure Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 116116Gamma-aminobutyric acid receptor-associated protein-like 2
PRO_0000212375Add
BLAST
Propeptidei117 – 1171Removed in mature form By similarity
PRO_0000423072

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei24 – 241N6-acetyllysine By similarity
Modified residuei39 – 391Phosphoserine By similarity
Lipidationi116 – 1161Phosphatidylethanolamine amidated glycine By similarity

Post-translational modificationi

The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAPL2-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAPL2-II. ATG4B also mediates the delipidation required for GABARAPL1 recycling when autophagosomes fuse with lysosomes By similarity.

Keywords - PTMi

Acetylation, Lipoprotein, Phosphoprotein

Proteomic databases

PRIDEiP60522.

Expressioni

Gene expression databases

GenevestigatoriP60522.

Interactioni

Subunit structurei

Monomer By similarity. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin By similarity. Interacts with ATG3, ATG7, ATG13 and ULK1 By similarity. Interacts with TP53INP1 and TP53INP2 By similarity. Interacts with TBC1D25 By similarity. Directly interacts with SQSTM1 and BNIP3 By similarity. Interacts with TECPR2 and PCM1 By similarity.

Structurei

3D structure databases

ProteinModelPortaliP60522.
SMRiP60522. Positions 1-117.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2222Interaction with beta-tubulin By similarity
Add
BLAST
Regioni36 – 6833Interaction with GABRG2 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the ATG8 family.

Phylogenomic databases

eggNOGiNOG249730.
GeneTreeiENSGT00390000012937.
HOGENOMiHOG000232034.
HOVERGENiHBG051706.
InParanoidiP60522.
KOiK08341.
OMAiHALEHRC.
OrthoDBiEOG70KGRK.
PhylomeDBiP60522.
TreeFamiTF312964.

Family and domain databases

InterProiIPR004241. Atg8_like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10969. PTHR10969. 1 hit.
PfamiPF02991. Atg8. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60522-1 [UniParc]FASTAAdd to Basket

« Hide

MKWMFKEDHS LEHRCVESAK IRAKYPDRVP VIVEKVSGSQ IVDIDKRKYL    50
VPSDITVAQF MWIIRKRIQL PSEKAIFLFV DKTVPQSSLT MGQLYEKEKD 100
EDGFLYVAYS GENTFGF 117
Length:117
Mass (Da):13,667
Last modified:March 1, 2004 - v1
Checksum:i17ACB540FD5B975B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB003515 mRNA. Translation: BAA19975.1.
BC058145 mRNA. Translation: AAH58145.1.
BC088139 mRNA. Translation: AAH88139.1.
RefSeqiNP_073197.1. NM_022706.2.
XP_002729993.3. XM_002729947.3.
UniGeneiRn.64537.

Genome annotation databases

EnsembliENSRNOT00000041209; ENSRNOP00000066243; ENSRNOG00000032756.
ENSRNOT00000048998; ENSRNOP00000040067; ENSRNOG00000019425.
GeneIDi100359937.
64670.
KEGGirno:100359937.
rno:64670.
UCSCiRGD:620510. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB003515 mRNA. Translation: BAA19975.1 .
BC058145 mRNA. Translation: AAH58145.1 .
BC088139 mRNA. Translation: AAH88139.1 .
RefSeqi NP_073197.1. NM_022706.2.
XP_002729993.3. XM_002729947.3.
UniGenei Rn.64537.

3D structure databases

ProteinModelPortali P60522.
SMRi P60522. Positions 1-117.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P60522.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000041209 ; ENSRNOP00000066243 ; ENSRNOG00000032756 .
ENSRNOT00000048998 ; ENSRNOP00000040067 ; ENSRNOG00000019425 .
GeneIDi 100359937.
64670.
KEGGi rno:100359937.
rno:64670.
UCSCi RGD:620510. rat.

Organism-specific databases

CTDi 11345.
RGDi 620510. Gabarapl2.

Phylogenomic databases

eggNOGi NOG249730.
GeneTreei ENSGT00390000012937.
HOGENOMi HOG000232034.
HOVERGENi HBG051706.
InParanoidi P60522.
KOi K08341.
OMAi HALEHRC.
OrthoDBi EOG70KGRK.
PhylomeDBi P60522.
TreeFami TF312964.

Miscellaneous databases

NextBioi 613665.
PROi P60522.

Gene expression databases

Genevestigatori P60522.

Family and domain databases

InterProi IPR004241. Atg8_like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
PANTHERi PTHR10969. PTHR10969. 1 hit.
Pfami PF02991. Atg8. 1 hit.
[Graphical view ]
SUPFAMi SSF54236. SSF54236. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression cloning of a cDNA encoding a ganglioside expression factor-2 (GEF-2)."
    Ogura K.
    Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver and Pituitary.

Entry informationi

Entry nameiGBRL2_RAT
AccessioniPrimary (citable) accession number: P60522
Secondary accession number(s): O08765, Q9DCP8, Q9UQF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: September 3, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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