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P60522

- GBRL2_RAT

UniProt

P60522 - GBRL2_RAT

Protein

Gamma-aminobutyric acid receptor-associated protein-like 2

Gene

Gabarapl2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
  1. Functioni

    Ubiquitin-like modifier involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1. Involved in autophagy. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei116 – 1172Cleavage; by ATG4BBy similarity

    GO - Biological processi

    1. autophagy Source: UniProtKB-KW
    2. protein transport Source: UniProtKB-KW

    Keywords - Biological processi

    Autophagy, Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Gamma-aminobutyric acid receptor-associated protein-like 2
    Alternative name(s):
    GABA(A) receptor-associated protein-like 2
    Ganglioside expression factor 2
    Short name:
    GEF-2
    Golgi-associated ATPase enhancer of 16 kDa
    Short name:
    GATE-16
    Gene namesi
    Name:Gabarapl2
    Synonyms:Gef2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 19, UP000002494: Chromosome 8

    Organism-specific databases

    RGDi620510. Gabarapl2.

    Subcellular locationi

    GO - Cellular componenti

    1. autophagic vacuole Source: UniProtKB
    2. cytoplasmic vesicle Source: UniProtKB-KW
    3. Golgi apparatus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 116116Gamma-aminobutyric acid receptor-associated protein-like 2PRO_0000212375Add
    BLAST
    Propeptidei117 – 1171Removed in mature formBy similarityPRO_0000423072

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei24 – 241N6-acetyllysineBy similarity
    Modified residuei39 – 391PhosphoserineBy similarity
    Lipidationi116 – 1161Phosphatidylethanolamine amidated glycineBy similarity

    Post-translational modificationi

    The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAPL2-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAPL2-II. ATG4B also mediates the delipidation required for GABARAPL1 recycling when autophagosomes fuse with lysosomes By similarity.By similarity

    Keywords - PTMi

    Acetylation, Lipoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiP60522.

    Expressioni

    Gene expression databases

    GenevestigatoriP60522.

    Interactioni

    Subunit structurei

    Monomer. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin. Interacts with ATG3, ATG7, ATG13 and ULK1. Interacts with TP53INP1 and TP53INP2. Interacts with TBC1D25. Directly interacts with SQSTM1 and BNIP3. Interacts with TECPR2 and PCM1.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP60522.
    SMRiP60522. Positions 1-117.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 2222Interaction with beta-tubulinBy similarityAdd
    BLAST
    Regioni36 – 6833Interaction with GABRG2Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ATG8 family.Curated

    Phylogenomic databases

    eggNOGiNOG249730.
    GeneTreeiENSGT00390000012937.
    HOGENOMiHOG000232034.
    HOVERGENiHBG051706.
    InParanoidiP60522.
    KOiK08341.
    OMAiHALEHRC.
    OrthoDBiEOG70KGRK.
    PhylomeDBiP60522.
    TreeFamiTF312964.

    Family and domain databases

    InterProiIPR004241. Atg8_like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PANTHERiPTHR10969. PTHR10969. 1 hit.
    PfamiPF02991. Atg8. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P60522-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKWMFKEDHS LEHRCVESAK IRAKYPDRVP VIVEKVSGSQ IVDIDKRKYL    50
    VPSDITVAQF MWIIRKRIQL PSEKAIFLFV DKTVPQSSLT MGQLYEKEKD 100
    EDGFLYVAYS GENTFGF 117
    Length:117
    Mass (Da):13,667
    Last modified:March 1, 2004 - v1
    Checksum:i17ACB540FD5B975B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB003515 mRNA. Translation: BAA19975.1.
    BC058145 mRNA. Translation: AAH58145.1.
    BC088139 mRNA. Translation: AAH88139.1.
    RefSeqiNP_073197.1. NM_022706.2.
    XP_002729993.3. XM_002729947.3.
    UniGeneiRn.64537.

    Genome annotation databases

    EnsembliENSRNOT00000041209; ENSRNOP00000066243; ENSRNOG00000032756.
    ENSRNOT00000048998; ENSRNOP00000040067; ENSRNOG00000019425.
    GeneIDi100359937.
    64670.
    KEGGirno:100359937.
    rno:64670.
    UCSCiRGD:620510. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB003515 mRNA. Translation: BAA19975.1 .
    BC058145 mRNA. Translation: AAH58145.1 .
    BC088139 mRNA. Translation: AAH88139.1 .
    RefSeqi NP_073197.1. NM_022706.2.
    XP_002729993.3. XM_002729947.3.
    UniGenei Rn.64537.

    3D structure databases

    ProteinModelPortali P60522.
    SMRi P60522. Positions 1-117.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P60522.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000041209 ; ENSRNOP00000066243 ; ENSRNOG00000032756 .
    ENSRNOT00000048998 ; ENSRNOP00000040067 ; ENSRNOG00000019425 .
    GeneIDi 100359937.
    64670.
    KEGGi rno:100359937.
    rno:64670.
    UCSCi RGD:620510. rat.

    Organism-specific databases

    CTDi 11345.
    RGDi 620510. Gabarapl2.

    Phylogenomic databases

    eggNOGi NOG249730.
    GeneTreei ENSGT00390000012937.
    HOGENOMi HOG000232034.
    HOVERGENi HBG051706.
    InParanoidi P60522.
    KOi K08341.
    OMAi HALEHRC.
    OrthoDBi EOG70KGRK.
    PhylomeDBi P60522.
    TreeFami TF312964.

    Miscellaneous databases

    NextBioi 613665.
    PROi P60522.

    Gene expression databases

    Genevestigatori P60522.

    Family and domain databases

    InterProi IPR004241. Atg8_like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    PANTHERi PTHR10969. PTHR10969. 1 hit.
    Pfami PF02991. Atg8. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Expression cloning of a cDNA encoding a ganglioside expression factor-2 (GEF-2)."
      Ogura K.
      Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver and Pituitary.

    Entry informationi

    Entry nameiGBRL2_RAT
    AccessioniPrimary (citable) accession number: P60522
    Secondary accession number(s): O08765, Q9DCP8, Q9UQF7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2004
    Last sequence update: March 1, 2004
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3