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Protein

Gamma-aminobutyric acid receptor-associated protein-like 2

Gene

GABARAPL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-like modifier involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 (By similarity). Involved in autophagy. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.By similarity2 Publications

GO - Molecular functioni

  • ATPase binding Source: UniProtKB
  • beta-tubulin binding Source: UniProtKB
  • GABA receptor binding Source: UniProtKB
  • microtubule binding Source: UniProtKB
  • SNARE binding Source: UniProtKB
  • ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

  • autophagosome assembly Source: GO_Central
  • autophagy Source: UniProtKB
  • cellular response to nitrogen starvation Source: GO_Central
  • intra-Golgi vesicle-mediated transport Source: UniProtKB
  • macroautophagy Source: Reactome
  • mitophagy Source: GO_Central
  • negative regulation of proteasomal protein catabolic process Source: BHF-UCL
  • positive regulation of ATPase activity Source: UniProtKB
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-1632852. Macroautophagy.
SIGNORiP60520.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor-associated protein-like 2
Alternative name(s):
GABA(A) receptor-associated protein-like 2
Ganglioside expression factor 2
Short name:
GEF-2
General protein transport factor p16
Golgi-associated ATPase enhancer of 16 kDa
Short name:
GATE-16
MAP1 light chain 3-related protein
Gene namesi
Name:GABARAPL2
Synonyms:FLC3A, GEF2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:13291. GABARAPL2.

Subcellular locationi

GO - Cellular componenti

  • autophagosome Source: UniProtKB
  • autophagosome membrane Source: UniProtKB
  • cytoplasm Source: BHF-UCL
  • cytoplasmic vesicle Source: UniProtKB-KW
  • cytosol Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • Golgi membrane Source: UniProtKB
  • intracellular Source: LIFEdb
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi116 – 1161G → A: Impairs localization at the autophagosomal membrane. 1 Publication

Organism-specific databases

PharmGKBiPA28482.

Polymorphism and mutation databases

BioMutaiGABARAPL2.
DMDMi44888808.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 116116Gamma-aminobutyric acid receptor-associated protein-like 2PRO_0000212373Add
BLAST
Propeptidei117 – 1171Removed in mature formPRO_0000423070

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei24 – 241N6-acetyllysineCombined sources
Modified residuei39 – 391PhosphoserineCombined sources
Lipidationi116 – 1161Phosphatidylethanolamine amidated glycine1 Publication

Post-translational modificationi

The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAPL2-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAPL2-II. ATG4B also mediates the delipidation required for GABARAPL1 recycling when autophagosomes fuse with lysosomes.1 Publication
The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei116 – 1172Cleavage; by ATG4

Keywords - PTMi

Acetylation, Lipoprotein, Phosphoprotein

Proteomic databases

EPDiP60520.
PaxDbiP60520.
PeptideAtlasiP60520.
PRIDEiP60520.

2D gel databases

UCD-2DPAGEP60520.

PTM databases

iPTMnetiP60520.
PhosphoSiteiP60520.

Miscellaneous databases

PMAP-CutDBP60520.

Expressioni

Tissue specificityi

Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine.2 Publications

Gene expression databases

BgeeiENSG00000034713.
CleanExiHS_GABARAPL2.
ExpressionAtlasiP60520. baseline and differential.
GenevisibleiP60520. HS.

Organism-specific databases

HPAiHPA036726.

Interactioni

Subunit structurei

Monomer. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin (By similarity). Interacts with ATG3, ATG7, ATG13 and ULK1. Interacts with TP53INP1 and TP53INP2. Interacts with TBC1D25. Directly interacts with SQSTM1 and BNIP3. Interacts with TECPR2 and PCM1. Interacts with TBC1D5 (PubMed:22354992). Interacts with TRIM5 (PubMed:25127057). Interacts with MEFV and TRIM21 (PubMed:26347139).By similarity16 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AGTRAPQ6RW133EBI-720116,EBI-741181
AHNAK2Q8IVF2-23EBI-720116,EBI-10217765
ANKFY1Q9P2R32EBI-720116,EBI-2513908
ARFGAP1Q8N6T33EBI-720116,EBI-716933
ATG13O751433EBI-720116,EBI-2798775
ATG16L1Q676U52EBI-720116,EBI-535909
ATG2AQ2TAZ02EBI-720116,EBI-2514077
ATG3Q9NT625EBI-720116,EBI-988094
ATG4BQ9Y4P17EBI-720116,EBI-712014
ATG7O953527EBI-720116,EBI-987834
BCL2L13Q9BXK54EBI-720116,EBI-747430
Bnip3lQ9Z2F72EBI-720116,EBI-1774669From a different organism.
CALCOCO1Q9P1Z23EBI-720116,EBI-749920
CALCOCO2Q131374EBI-720116,EBI-739580
CCDC155Q8N6L03EBI-720116,EBI-749265
DYX1C1Q8WXU22EBI-720116,EBI-2946907
EGFRP005332EBI-720116,EBI-297353
FUNDC1Q8IVP53EBI-720116,EBI-3059266
FYCO1Q9BQS82EBI-720116,EBI-2869338
GBASO753236EBI-720116,EBI-307133
HADHAP409394EBI-720116,EBI-356720
IPO5O004105EBI-720116,EBI-356424
KBTBD6Q86V972EBI-720116,EBI-2514778
KBTBD7Q8WVZ93EBI-720116,EBI-473695
KIAA1958Q8N8K93EBI-720116,EBI-10181113
KRTAP10-7P604093EBI-720116,EBI-10172290
KRTAP10-9P604113EBI-720116,EBI-10172052
MLXQ9UH924EBI-720116,EBI-741109
NBR1Q1459611EBI-720116,EBI-742698
NEDD4P469346EBI-720116,EBI-726944
NEK9Q8TD197EBI-720116,EBI-1044009
RASSF1Q9NS232EBI-720116,EBI-367363
RASSF5Q8WWW02EBI-720116,EBI-367390
RCN2Q142573EBI-720116,EBI-356710
SIK2Q9H0K13EBI-720116,EBI-1181664
SQSTM1Q1350115EBI-720116,EBI-307104
STBD1O952105EBI-720116,EBI-2947137
STK3Q131882EBI-720116,EBI-992580
STK4Q130432EBI-720116,EBI-367376
TBC1D15Q8TC072EBI-720116,EBI-1048247
TBC1D2BQ9UPU73EBI-720116,EBI-2947180
TBC1D5B9A6K13EBI-720116,EBI-10217641
TBC1D9BQ66K14-23EBI-720116,EBI-10217736
TECPR2O150402EBI-720116,EBI-2946991
TNIP1Q150253EBI-720116,EBI-357849
TSR2Q969E85EBI-720116,EBI-746981
UBA5Q9GZZ913EBI-720116,EBI-747805
ULK1O753853EBI-720116,EBI-908831
WDFY3Q8IZQ13EBI-720116,EBI-1569256

GO - Molecular functioni

  • ATPase binding Source: UniProtKB
  • beta-tubulin binding Source: UniProtKB
  • GABA receptor binding Source: UniProtKB
  • microtubule binding Source: UniProtKB
  • SNARE binding Source: UniProtKB
  • ubiquitin protein ligase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi116473. 82 interactions.
DIPiDIP-35051N.
IntActiP60520. 509 interactions.
MINTiMINT-1414121.
STRINGi9606.ENSP00000037243.

Structurei

Secondary structure

1
117
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 85Combined sources
Helixi11 – 2414Combined sources
Beta strandi28 – 358Combined sources
Beta strandi48 – 525Combined sources
Helixi57 – 6711Combined sources
Beta strandi77 – 804Combined sources
Helixi91 – 988Combined sources
Beta strandi105 – 1117Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4CO7X-ray2.00A/B1-117[»]
ProteinModelPortaliP60520.
SMRiP60520. Positions 1-117.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2222Interaction with beta-tubulinBy similarityAdd
BLAST
Regioni36 – 6833Interaction with GABRG2Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the ATG8 family.Curated

Phylogenomic databases

eggNOGiKOG1654. Eukaryota.
ENOG4111JAT. LUCA.
GeneTreeiENSGT00390000012937.
HOGENOMiHOG000232034.
HOVERGENiHBG051706.
InParanoidiP60520.
KOiK08341.
OMAiSEHRCVE.
OrthoDBiEOG091G10QX.
PhylomeDBiP60520.
TreeFamiTF312964.

Family and domain databases

CDDicd01611. GABARAP. 1 hit.
InterProiIPR004241. Atg8-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10969. PTHR10969. 1 hit.
PfamiPF02991. Atg8. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60520-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKWMFKEDHS LEHRCVESAK IRAKYPDRVP VIVEKVSGSQ IVDIDKRKYL
60 70 80 90 100
VPSDITVAQF MWIIRKRIQL PSEKAIFLFV DKTVPQSSLT MGQLYEKEKD
110
EDGFLYVAYS GENTFGF
Length:117
Mass (Da):13,667
Last modified:March 1, 2004 - v1
Checksum:i17ACB540FD5B975B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 291V → F in AAD27779 (Ref. 4) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti51 – 511V → A.
Corresponds to variant rs11556291 [ dbSNP | Ensembl ].
VAR_049756

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB030710 mRNA. Translation: BAB21548.1.
AF087848 mRNA. Translation: AAK20400.1.
AJ010569 mRNA. Translation: CAA09249.1.
AF077046 mRNA. Translation: AAD27779.1.
CR542217 mRNA. Translation: CAG47013.1.
AK289788 mRNA. Translation: BAF82477.1.
CH471114 Genomic DNA. Translation: EAW95630.1.
BC005985 mRNA. Translation: AAH05985.1.
BC014594 mRNA. Translation: AAH14594.1.
BC029601 mRNA. Translation: AAH29601.1.
CCDSiCCDS10921.1.
RefSeqiNP_009216.1. NM_007285.6.
UniGeneiHs.461379.

Genome annotation databases

EnsembliENST00000037243; ENSP00000037243; ENSG00000034713.
GeneIDi11345.
KEGGihsa:11345.
UCSCiuc002fen.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB030710 mRNA. Translation: BAB21548.1.
AF087848 mRNA. Translation: AAK20400.1.
AJ010569 mRNA. Translation: CAA09249.1.
AF077046 mRNA. Translation: AAD27779.1.
CR542217 mRNA. Translation: CAG47013.1.
AK289788 mRNA. Translation: BAF82477.1.
CH471114 Genomic DNA. Translation: EAW95630.1.
BC005985 mRNA. Translation: AAH05985.1.
BC014594 mRNA. Translation: AAH14594.1.
BC029601 mRNA. Translation: AAH29601.1.
CCDSiCCDS10921.1.
RefSeqiNP_009216.1. NM_007285.6.
UniGeneiHs.461379.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4CO7X-ray2.00A/B1-117[»]
ProteinModelPortaliP60520.
SMRiP60520. Positions 1-117.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116473. 82 interactions.
DIPiDIP-35051N.
IntActiP60520. 509 interactions.
MINTiMINT-1414121.
STRINGi9606.ENSP00000037243.

PTM databases

iPTMnetiP60520.
PhosphoSiteiP60520.

Polymorphism and mutation databases

BioMutaiGABARAPL2.
DMDMi44888808.

2D gel databases

UCD-2DPAGEP60520.

Proteomic databases

EPDiP60520.
PaxDbiP60520.
PeptideAtlasiP60520.
PRIDEiP60520.

Protocols and materials databases

DNASUi11345.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000037243; ENSP00000037243; ENSG00000034713.
GeneIDi11345.
KEGGihsa:11345.
UCSCiuc002fen.3. human.

Organism-specific databases

CTDi11345.
GeneCardsiGABARAPL2.
HGNCiHGNC:13291. GABARAPL2.
HPAiHPA036726.
MIMi607452. gene.
neXtProtiNX_P60520.
PharmGKBiPA28482.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1654. Eukaryota.
ENOG4111JAT. LUCA.
GeneTreeiENSGT00390000012937.
HOGENOMiHOG000232034.
HOVERGENiHBG051706.
InParanoidiP60520.
KOiK08341.
OMAiSEHRCVE.
OrthoDBiEOG091G10QX.
PhylomeDBiP60520.
TreeFamiTF312964.

Enzyme and pathway databases

ReactomeiR-HSA-1632852. Macroautophagy.
SIGNORiP60520.

Miscellaneous databases

ChiTaRSiGABARAPL2. human.
GeneWikiiGABARAPL2.
GenomeRNAii11345.
PMAP-CutDBP60520.
PROiP60520.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000034713.
CleanExiHS_GABARAPL2.
ExpressionAtlasiP60520. baseline and differential.
GenevisibleiP60520. HS.

Family and domain databases

CDDicd01611. GABARAP. 1 hit.
InterProiIPR004241. Atg8-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10969. PTHR10969. 1 hit.
PfamiPF02991. Atg8. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGBRL2_HUMAN
AccessioniPrimary (citable) accession number: P60520
Secondary accession number(s): O08765
, Q6FG91, Q9DCP8, Q9UQF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: September 7, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.