P60520 (GBRL2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-aminobutyric acid receptor-associated protein-like 2

Alternative name(s):
GABA(A) receptor-associated protein-like 2
Ganglioside expression factor 2
Short name=GEF-2
General protein transport factor p16
Golgi-associated ATPase enhancer of 16 kDa
Short name=GATE-16
MAP1 light chain 3-related protein

Gene names

Name:	GABARAPL2
Synonyms:	FLC3A, GEF2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	117 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 By similarity. Involved in autophagy By similarity.
Subunit structure	Monomer. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin By similarity. Interacts with ULK1. Interacts with TP53INP1 and TP53INP2. Interacts with TBC1D25. Directly interacts with SQSTM1. Ref.1 Ref.9 Ref.11 Ref.14 Ref.15 Ref.16
Subcellular location	Golgi apparatus By similarity. Cytoplasmic vesicle › autophagosome Ref.9 Ref.11.
Tissue specificity	Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine. Ref.1 Ref.2
Sequence similarities	Belongs to the MAP1 LC3 family.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport
Cellular component	Cytoplasmic vesicle Golgi apparatus
Coding sequence diversity	Polymorphism
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	autophagy Non-traceable author statement PubMed 12507496. Source: UniProtKB intra-Golgi vesicle-mediated transport Inferred from sequence or structural similarity PubMed 10747018. Source: UniProtKB negative regulation of protein catabolic process Inferred from mutant phenotype PubMed 21669198. Source: BHF-UCL positive regulation of ATPase activity Inferred from sequence or structural similarity PubMed 10747018. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi membrane Inferred from sequence or structural similarity PubMed 10747018Ref.2. Source: UniProtKB autophagic vacuole membrane Inferred from direct assay PubMed 15169837. Source: UniProtKB cytoplasmic vesicle Inferred from electronic annotation. Source: UniProtKB-KW cytosol Inferred from sequence or structural similarity Ref.2. Source: UniProtKB
Molecular_function	ATPase binding Inferred from sequence or structural similarity PubMed 10747018. Source: UniProtKB GABA receptor binding Inferred from sequence or structural similarity. Source: UniProtKB SNARE binding Inferred from sequence or structural similarity PubMed 10747018. Source: UniProtKB beta-tubulin binding Inferred from sequence or structural similarity. Source: UniProtKB microtubule binding Non-traceable author statement Ref.2. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ANKFY1	Q9P2R3	2	EBI-720116,EBI-2513908
ATG13	O75143	2	EBI-720116,EBI-2798775
ATG16L1	Q676U5	2	EBI-720116,EBI-535909
ATG2A	Q2TAZ0	2	EBI-720116,EBI-2514077
ATG3	Q9NT62	5	EBI-720116,EBI-988094
ATG4B	Q9Y4P1	6	EBI-720116,EBI-712014
ATG7	O95352	7	EBI-720116,EBI-987834
DYX1C1	Q8WXU2	2	EBI-720116,EBI-2946907
EGFR	P00533	2	EBI-720116,EBI-297353
FYCO1	Q9BQS8	2	EBI-720116,EBI-2869338
GBAS	O75323	6	EBI-720116,EBI-307133
HADHA	P40939	4	EBI-720116,EBI-356720
IPO5	O00410	5	EBI-720116,EBI-356424
KBTBD6	Q86V97	2	EBI-720116,EBI-2514778
KBTBD7	Q8WVZ9	2	EBI-720116,EBI-473695
NBR1	Q14596	11	EBI-720116,EBI-742698
NEDD4	P46934	6	EBI-720116,EBI-726944
NEK9	Q8TD19	7	EBI-720116,EBI-1044009
RASSF1	Q9NS23	2	EBI-720116,EBI-367363
RASSF5	Q8WWW0	2	EBI-720116,EBI-367390
SQSTM1	Q13501	11	EBI-720116,EBI-307104
STBD1	O95210	5	EBI-720116,EBI-2947137
STK3	Q13188	2	EBI-720116,EBI-992580
STK4	Q13043	2	EBI-720116,EBI-367376
TBC1D15	Q8TC07	2	EBI-720116,EBI-1048247
TBC1D2B	Q9UPU7	3	EBI-720116,EBI-2947180
TECPR2	O15040	2	EBI-720116,EBI-2946991
UBA5	Q9GZZ9	9	EBI-720116,EBI-747805
ULK1	O75385	3	EBI-720116,EBI-908831

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 117	117	Gamma-aminobutyric acid receptor-associated protein-like 2		PRO_0000212373
Regions
	Region	1 – 22	22	Interaction with beta-tubulin By similarity
	Region	36 – 68	33	Interaction with GABRG2 Potential
Amino acid modifications
	Modified residue	24	1	N6-acetyllysine Ref.12
	Modified residue	39	1	Phosphoserine Ref.10
Natural variations
	Natural variant	51	1	V → A. Corresponds to variant rs11556291 [ dbSNP | Ensembl ].		VAR_049756
Experimental info
	Sequence conflict	29	1	V → F in AAD27779. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P60520 [UniParc]. Last modified March 1, 2004. Version 1. Checksum: 17ACB540FD5B975B	FASTA	117	13,667
	10 20 30 40 50 60 MKWMFKEDHS LEHRCVESAK IRAKYPDRVP VIVEKVSGSQ IVDIDKRKYL VPSDITVAQF 70 80 90 100 110 MWIIRKRIQL PSEKAIFLFV DKTVPQSSLT MGQLYEKEKD EDGFLYVAYS GENTFGF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Interaction of the Unc-51-like kinase and microtubule-associated protein light chain 3 related proteins in the brain: possible role of vesicular transport in axonal elongation." Okazaki N., Yan J., Yuasa S., Ueno T., Kominami E., Masuho Y., Koga H., Muramatsu M.-A. Brain Res. Mol. Brain Res. 85:1-12(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH ULK1. Tissue: Frontal cortex.
[2]	"Cloning, expression patterns, and chromosome localization of three human and two mouse homologues of GABA(A) receptor-associated protein." Xin Y., Yu L., Chen Z., Zheng L., Fu Q., Jiang J., Zhang P., Gong R., Zhao S. Genomics 74:408-413(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[3]	Storch S., Braulke T. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Human GEF2 homolog gene." Song H., Peng Y., Yu Y., Fu G., Mao M., Zhang Q., Zhu H., Li G., Luo M., Chen J., Hu R. Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pituitary.
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Testis.
[9]	"p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy." Pankiv S., Clausen T.H., Lamark T., Brech A., Bruun J.A., Outzen H., Overvatn A., Bjorkoy G., Johansen T. J. Biol. Chem. 282:24131-24145(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SQSTM1, SUBCELLULAR LOCATION.
[10]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[11]	"The TP53INP2 protein is required for autophagy in mammalian cells." Nowak J., Archange C., Tardivel-Lacombe J., Pontarotti P., Pebusque M.J., Vaccaro M.I., Velasco G., Dagorn J.C., Iovanna J.L. Mol. Biol. Cell 20:870-881(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH TP53INP2.
[12]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-24, MASS SPECTROMETRY.
[13]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]	"OATL1, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation." Itoh T., Kanno E., Uemura T., Waguri S., Fukuda M. J. Cell Biol. 192:839-853(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TBC1D25.
[15]	"TP53INP1, a tumor suppressor, interacts with LC3 and ATG8-family proteins through the LC3-interacting region (LIR) and promotes autophagy-dependent cell death." Seillier M., Peuget S., Gayet O., Gauthier C., N'guessan P., Monte M., Carrier A., Iovanna J.L., Dusetti N.J. Cell Death Differ. 19:1525-1535(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TP53INP1.
[16]	"DOR/Tp53inp2 and Tp53inp1 constitute a metazoan gene family encoding dual regulators of autophagy and transcription." Sancho A., Duran J., Garcia-Espana A., Mauvezin C., Alemu E.A., Lamark T., Macias M.J., Desalle R., Royo M., Sala D., Chicote J.U., Palacin M., Johansen T., Zorzano A. PLoS ONE 7:E34034-E34034(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TP53INP1 AND TP53INP2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB030710 mRNA. Translation: BAB21548.1. AF087848 mRNA. Translation: AAK20400.1. AJ010569 mRNA. Translation: CAA09249.1. AF077046 mRNA. Translation: AAD27779.1. CR542217 mRNA. Translation: CAG47013.1. AK289788 mRNA. Translation: BAF82477.1. CH471114 Genomic DNA. Translation: EAW95630.1. BC005985 mRNA. Translation: AAH05985.1. BC014594 mRNA. Translation: AAH14594.1. BC029601 mRNA. Translation: AAH29601.1.
IPI	IPI00026358.
RefSeq	NP_009216.1. NM_007285.6.
UniGene	Hs.461379.
3D structure databases
ProteinModelPortal	P60520.
ModBase	Search...
Protein-protein interaction databases
IntAct	P60520. 489 interactions.
MINT	MINT-1414121.
STRING	9606.ENSP00000037243.
PTM databases
PhosphoSite	P60520.
Polymorphism databases
DMDM	44888808.
2D gel databases
UCD-2DPAGE	P60520.
Proteomic databases
PaxDb	P60520.
PRIDE	P60520.
Protocols and materials databases
DNASU	11345.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000037243; ENSP00000037243; ENSG00000034713.
GeneID	11345.
KEGG	hsa:11345.
UCSC	uc002fen.3. human.
Organism-specific databases
CTD	11345.
GeneCards	GC16P075600.
HGNC	HGNC:13291. GABARAPL2.
HPA	HPA036726.
MIM	607452. gene.
neXtProt	NX_P60520.
PharmGKB	PA28482.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG249730.
HOGENOM	HOG000232034.
HOVERGEN	HBG051706.
InParanoid	P60520.
KO	K08341.
OMA	IRRRITM.
OrthoDB	EOG4V4394.
Gene expression databases
ArrayExpress	P60520.
Bgee	P60520.
CleanEx	HS_GABARAPL2.
Genevestigator	P60520.
GermOnline	ENSG00000034713. Homo sapiens.
Family and domain databases
InterPro	IPR004241. Atg8_ubiquitin-like. [Graphical view]
PANTHER	PTHR10969. PTHR10969. 1 hit.
Pfam	PF02991. Atg8. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	GABARAPL2. human.
GenomeRNAi	11345.
NextBio	43116.
PMAP-CutDB	P60520.
SOURCE	Search...

Entry information

Entry name

GBRL2_HUMAN

Accession

Primary (citable) accession number: P60520
Secondary accession number(s): O08765 , Q6FG91, Q9DCP8, Q9UQF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	March 1, 2004
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.