Reviewed, UniProtKB/Swiss-Prot P60520 (GBRL2_HUMAN)

Names and origin

Protein names

Recommended name:
    Gamma-aminobutyric acid receptor-associated protein-like 2
Alternative name(s):
    GABA(A) receptor-associated protein-like 2
    Golgi-associated ATPase enhancer of 16 kDa
      Short name=GATE-16
    General protein transport factor p16
    Ganglioside expression factor 2
      Short name=GEF-2
    MAP1 light chain 3-related protein

Gene names

Name:	GABARAPL2
Synonyms:	FLC3A, GEF2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	117 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 By similarity.
Subunit structure	Monomer. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin By similarity. Interacts with ULK1.
Subcellular location	Golgi apparatusBy similarity.
Tissue specificity	Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR.
Sequence similarities	Belongs to the MAP1 LC3 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	autophagy Non-traceable author statement. Source: UniProtKB intra-Golgi vesicle-mediated transport Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of ATPase activity Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	Golgi membrane Ref.2 Inferred from sequence or structural similarity. Source: UniProtKB autophagic vacuole membrane Inferred from direct assay. Source: UniProtKB cytosol Ref.2 Inferred from sequence or structural similarity. Source: UniProtKB membrane fraction Inferred from direct assay. Source: UniProtKB
Molecular function	ATPase binding Inferred from sequence or structural similarity. Source: UniProtKB GABA receptor binding Ref.2 Non-traceable author statement. Source: UniProtKB SNARE binding Inferred from sequence or structural similarity. Source: UniProtKB beta-tubulin binding Inferred from sequence or structural similarity. Source: UniProtKB microtubule binding Ref.2 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATG3	Q9NT62	1	EBI-720116,EBI-988094
ATG4B	Q9Y4P1	1	EBI-720116,EBI-712014
EIF4G1	Q04637	1	EBI-720116,EBI-73711
HBG1	P69891	1	EBI-720116,EBI-1056789
IRGQ	Q8WZA9	1	EBI-720116,EBI-1055331
LRPPRC	P42704	1	EBI-720116,EBI-1050853
NEK9	Q8TD19	1	EBI-720116,EBI-1044009
VDAC1	P21796	1	EBI-720116,EBI-354158
VIL2	P15311	1	EBI-720116,EBI-1056902

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 117	117	Gamma-aminobutyric acid receptor-associated protein-like 2
Regions
	Region	1 – 22	22	Interaction with beta-tubulin By similarity
	Region	36 – 68	33	Interaction with GABRG2 Potential
Amino acid modifications
	Modified residue	10	1	Phosphoserine
	Modified residue	39	1	Phosphoserine
Experimental info
	Sequence conflict	29	1	V → F in AAD27779. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P60520-1 [UniParc]. Last modified March 1, 2004. Version 1. Checksum: 17ACB540FD5B975B	FASTA	117	13,667
	10 20 30 40 50 60 MKWMFKEDHS LEHRCVESAK IRAKYPDRVP VIVEKVSGSQ IVDIDKRKYL VPSDITVAQF 70 80 90 100 110 MWIIRKRIQL PSEKAIFLFV DKTVPQSSLT MGQLYEKEKD EDGFLYVAYS GENTFGF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Interaction of the Unc-51-like kinase and microtubule-associated protein light chain 3 related proteins in the brain: possible role of vesicular transport in axonal elongation." Okazaki N., Yan J., Yuasa S., Ueno T., Kominami E., Masuho Y., Koga H., Muramatsu M.-A. Brain Res. Mol. Brain Res. 85:1-12(2000) [PubMed: 11146101] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH ULK1. Tissue: Frontal cortex.
[2]	"Cloning, expression patterns, and chromosome localization of three human and two mouse homologues of GABA(A) receptor-associated protein." Xin Y., Yu L., Chen Z., Zheng L., Fu Q., Jiang J., Zhang P., Gong R., Zhao S. Genomics 74:408-413(2001) [PubMed: 11414770] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[3]	Storch S., Braulke T. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Human GEF2 homolog gene." Song H., Peng Y., Yu Y., Fu G., Mao M., Zhang Q., Zhu H., Li G., Luo M., Chen J., Hu R. Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pituitary.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Testis.
[6]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, MASS SPECTROMETRY.
[7]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB030710 mRNA. Translation: BAB21548.1. AF087848 mRNA. Translation: AAK20400.1. AJ010569 mRNA. Translation: CAA09249.1. AF077046 mRNA. Translation: AAD27779.1. BC005985 mRNA. Translation: AAH05985.1. BC014594 mRNA. Translation: AAH14594.1. BC029601 mRNA. Translation: AAH29601.1.
RefSeq	NP_009216.1.
UniGene	Hs.461379
3D structure databases
SMR	P60520. Positions 1-117.
ModBase	Search...
Protein-protein interaction databases
IntAct	P60520.
PTM databases
PhosphoSite	P60520.
Genome annotation databases
Ensembl	ENSG00000034713. Homo sapiens. [Contig view]
GeneID	11345.
KEGG	hsa:11345.
Organism-specific databases
H-InvDB	HIX0013248.
HGNC	HGNC:13291. GABARAPL2.
MIM	607452. gene.
PharmGKB	PA28482.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	P60520.
HOVERGEN	P60520.
Gene expression databases
ArrayExpress	P60520.
CleanEx	HS_GABARAPL2.
GermOnline	ENSG00000034713. Homo sapiens.
Family and domain databases
InterPro	IPR004241. MAP1_LC3. [Graphical view]
PANTHER	PTHR10969. MAP1_LC3. 1 hit.
Pfam	PF02991. MAP1_LC3. 1 hit. [Graphical view]
ProDom	P60520. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

GBRL2_HUMAN

Accession

Primary (citable) accession number: P60520
Secondary accession number(s): O08765, Q9DCP8, Q9UQF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	March 1, 2004
Last modified:	July 22, 2008
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)