Gamma-aminobutyric acid receptor-associated protein-like 2

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Reviewed, UniProtKB/Swiss-Prot P60520 (GBRL2_HUMAN)

Last modified November 3, 2009. Version 54. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Gamma-aminobutyric acid receptor-associated protein-like 2
Alternative name(s):
    GABA(A) receptor-associated protein-like 2
    Golgi-associated ATPase enhancer of 16 kDa
      Short name=GATE-16
    General protein transport factor p16
    Ganglioside expression factor 2
      Short name=GEF-2
    MAP1 light chain 3-related protein

Gene names

Name:	GABARAPL2
Synonyms:	FLC3A, GEF2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	117 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 By similarity.
Subunit structure	Monomer. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin By similarity. Interacts with ULK1.
Subcellular location	Golgi apparatus By similarity.
Tissue specificity	Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine. Ref.1 Ref.2
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.6 Ref.7
Sequence similarities	Belongs to the MAP1 LC3 family.

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Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus
Coding sequence diversity	Polymorphism
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Non-traceable author statement. Source: UniProtKB intra-Golgi vesicle-mediated transport Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of ATPase activity Inferred from sequence or structural similarity. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi membrane Ref.2 Inferred from sequence or structural similarity. Source: UniProtKB autophagic vacuole membrane Inferred from direct assay. Source: UniProtKB cytosol Ref.2 Inferred from sequence or structural similarity. Source: UniProtKB membrane fraction Inferred from direct assay. Source: UniProtKB
Molecular function	ATPase binding Inferred from sequence or structural similarity. Source: UniProtKB GABA receptor binding Ref.2 Non-traceable author statement. Source: UniProtKB SNARE binding Inferred from sequence or structural similarity. Source: UniProtKB beta-tubulin binding Inferred from sequence or structural similarity. Source: UniProtKB microtubule binding Ref.2 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
ATG3	Q9NT62	1	EBI-720116,EBI-988094
ATG4B	Q9Y4P1	1	EBI-720116,EBI-712014
EIF4G1	Q04637	1	EBI-720116,EBI-73711
EZR	P15311	1	EBI-720116,EBI-1056902
HBG1	P69891	1	EBI-720116,EBI-1056789
IRGQ	Q8WZA9	1	EBI-720116,EBI-1055331
LRPPRC	P42704	1	EBI-720116,EBI-1050853
NEK9	Q8TD19	1	EBI-720116,EBI-1044009
VDAC1	P21796	1	EBI-720116,EBI-354158

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 117

117

Gamma-aminobutyric acid receptor-associated protein-like 2

PRO_0000212373

Regions

Region

1 – 22

Interaction with beta-tubulin By similarity

Region

36 – 68

Interaction with GABRG2 Potential

Amino acid modifications

Modified residue

Phosphoserine Ref.6

Modified residue

N6-acetyllysine Ref.9

Modified residue

Phosphoserine Ref.7

Natural variations

Natural variant

V → A: dbSNP rs11556291.

VAR_049756

Experimental info

Sequence conflict

V → F in AAD27779. Ref.4

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Sequences

Sequence

Length

Mass (Da)

Tools

P60520-1 [UniParc].

Last modified March 1, 2004. Version 1.
Checksum: 17ACB540FD5B975B
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FASTA

117

13,667

        10         20         30         40         50         60 
MKWMFKEDHS LEHRCVESAK IRAKYPDRVP VIVEKVSGSQ IVDIDKRKYL VPSDITVAQF 

        70         80         90        100        110 
MWIIRKRIQL PSEKAIFLFV DKTVPQSSLT MGQLYEKEKD EDGFLYVAYS GENTFGF

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Interaction of the Unc-51-like kinase and microtubule-associated protein light chain 3 related proteins in the brain: possible role of vesicular transport in axonal elongation." Okazaki N., Yan J., Yuasa S., Ueno T., Kominami E., Masuho Y., Koga H., Muramatsu M.-A. Brain Res. Mol. Brain Res. 85:1-12(2000) [PubMed: 11146101] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH ULK1. Tissue: Frontal cortex.
[2]	"Cloning, expression patterns, and chromosome localization of three human and two mouse homologues of GABA(A) receptor-associated protein." Xin Y., Yu L., Chen Z., Zheng L., Fu Q., Jiang J., Zhang P., Gong R., Zhao S. Genomics 74:408-413(2001) [PubMed: 11414770] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[3]	Storch S., Braulke T. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Human GEF2 homolog gene." Song H., Peng Y., Yu Y., Fu G., Mao M., Zhang Q., Zhu H., Li G., Luo M., Chen J., Hu R. Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pituitary.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Testis.
[6]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, MASS SPECTROMETRY.
[7]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39, MASS SPECTROMETRY.
[8]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[9]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-24, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	AB030710 mRNA. Translation: BAB21548.1. AF087848 mRNA. Translation: AAK20400.1. AJ010569 mRNA. Translation: CAA09249.1. AF077046 mRNA. Translation: AAD27779.1. BC005985 mRNA. Translation: AAH05985.1. BC014594 mRNA. Translation: AAH14594.1. BC029601 mRNA. Translation: AAH29601.1.
IPI	IPI00026358.
RefSeq	NP_009216.1.
UniGene	Hs.461379
3D structure databases
SMR	P60520. Positions 1-117.
ModBase	Search...
Protein-protein interaction databases
IntAct	P60520. 56 interactions.
STRING	P60520.
PTM databases
PhosphoSite	P60520.
Proteomic databases
PRIDE	P60520.
Genome annotation databases
Ensembl	ENST00000037243; ENSP00000037243; ENSG00000034713; Homo sapiens. [Genome view]
GeneID	11345.
KEGG	hsa:11345.
UCSC	uc002fen.1. human.
Organism-specific databases
CTD	11345.
GeneCards	GC16P074157.
H-InvDB	HIX0013248.
HGNC	HGNC:13291. GABARAPL2.
MIM	607452. gene.
PharmGKB	PA28482.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P60520.
HOVERGEN	P60520.
OMA	VICEKVE.
Gene expression databases
ArrayExpress	P60520.
Bgee	P60520.
CleanEx	HS_GABARAPL2.
Genevestigator	P60520.
GermOnline	ENSG00000034713. Homo sapiens.
Family and domain databases
InterPro	IPR004241. MAP1_LC3. [Graphical view]
PANTHER	PTHR10969. MAP1_LC3. 1 hit.
Pfam	PF02991. MAP1_LC3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	43116.
PMAP-CutDB	P60520.
SOURCE	Search...

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Entry information

Entry name

GBRL2_HUMAN

Accession

Primary (citable) accession number: P60520
Secondary accession number(s): O08765, Q9DCP8, Q9UQF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	March 1, 2004
Last modified:	November 3, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Index of human polymorphisms and disease mutations

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SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents