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Protein

Gamma-aminobutyric acid receptor-associated protein-like 2

Gene

GABARAPL2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-like modifier involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 (By similarity). Involved in autophagy. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (By similarity).By similarity1 Publication

GO - Molecular functioni

  • ATPase binding Source: UniProtKB
  • SNARE binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor-associated protein-like 2
Alternative name(s):
GABA(A) receptor-associated protein-like 2
Ganglioside expression factor 2
Short name:
GEF-2
General protein transport factor p16
Golgi-associated ATPase enhancer of 16 kDa
Short name:
GATE-16
MAP1 light chain 3-related protein
Gene namesi
Name:GABARAPL2
Synonyms:GEF2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  • autophagosome Source: UniProtKB
  • autophagosome membrane Source: GO_Central
  • cytoplasmic vesicle Source: UniProtKB-KW
  • cytosol Source: UniProtKB
  • Golgi membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 116116Gamma-aminobutyric acid receptor-associated protein-like 2PRO_0000212372Add
BLAST
Propeptidei117 – 1171Removed in mature formBy similarityPRO_0000423069

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei24 – 241N6-acetyllysineBy similarity
Modified residuei39 – 391PhosphoserineBy similarity
Lipidationi116 – 1161Phosphatidylethanolamine amidated glycineBy similarity

Post-translational modificationi

The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAPL2-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAPL2-II. ATG4B also mediates the delipidation required for GABARAPL2 recycling when autophagosomes fuse with lysosomes (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei116 – 1172Cleavage; by ATG4By similarity

Keywords - PTMi

Acetylation, Lipoprotein, Phosphoprotein

Proteomic databases

PaxDbiP60519.
PRIDEiP60519.

Expressioni

Tissue specificityi

Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine.1 Publication

Interactioni

Subunit structurei

Monomer. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin. Interacts with ATG3, ATG7, ATG13 and ULK1. Interacts with TP53INP1 and TP53INP2. Interacts with TBC1D25. Directly interacts with SQSTM1 and BNIP3. Interacts with TECPR2 and PCM1. Interacts with TBC1D5. Interacts with TRIM5. Interacts with MEFV and TRIM21.By similarity

GO - Molecular functioni

  • ATPase binding Source: UniProtKB
  • SNARE binding Source: UniProtKB

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000008607.

Structurei

Secondary structure

1
117
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 85Combined sources
Helixi11 – 2414Combined sources
Beta strandi28 – 358Combined sources
Beta strandi48 – 525Combined sources
Helixi57 – 6812Combined sources
Beta strandi77 – 804Combined sources
Helixi91 – 988Combined sources
Beta strandi105 – 1106Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EO6X-ray1.80A/B1-117[»]
ProteinModelPortaliP60519.
SMRiP60519. Positions 1-117.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60519.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2222Interaction with beta-tubulinBy similarityAdd
BLAST
Regioni36 – 6833Interaction with GABRG2Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the ATG8 family.Curated

Phylogenomic databases

eggNOGiKOG1654. Eukaryota.
ENOG4111JAT. LUCA.
HOGENOMiHOG000232034.
HOVERGENiHBG051706.
InParanoidiP60519.
KOiK08341.

Family and domain databases

InterProiIPR004241. Atg8-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10969. PTHR10969. 1 hit.
PfamiPF02991. Atg8. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60519-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKWMFKEDHS LEHRCVESAK IRAKYPDRVP VIVEKVSGSQ IVDIDKRKYL
60 70 80 90 100
VPSDITVAQF MWIIRKRIQL PSEKAIFLFV DKTVPQSSLT MGQLYEKEKD
110
EDGFLYVAYS GENTFGF
Length:117
Mass (Da):13,667
Last modified:March 1, 2004 - v1
Checksum:i17ACB540FD5B975B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020262 mRNA. Translation: AAD20720.1.
AY117147 mRNA. Translation: AAM77036.1.
BT020958 mRNA. Translation: AAX08975.1.
BC102902 mRNA. Translation: AAI02903.2.
RefSeqiNP_777100.1. NM_174675.2.
UniGeneiBt.41007.

Genome annotation databases

GeneIDi282531.
KEGGibta:282531.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020262 mRNA. Translation: AAD20720.1.
AY117147 mRNA. Translation: AAM77036.1.
BT020958 mRNA. Translation: AAX08975.1.
BC102902 mRNA. Translation: AAI02903.2.
RefSeqiNP_777100.1. NM_174675.2.
UniGeneiBt.41007.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EO6X-ray1.80A/B1-117[»]
ProteinModelPortaliP60519.
SMRiP60519. Positions 1-117.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000008607.

Proteomic databases

PaxDbiP60519.
PRIDEiP60519.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi282531.
KEGGibta:282531.

Organism-specific databases

CTDi11345.

Phylogenomic databases

eggNOGiKOG1654. Eukaryota.
ENOG4111JAT. LUCA.
HOGENOMiHOG000232034.
HOVERGENiHBG051706.
InParanoidiP60519.
KOiK08341.

Miscellaneous databases

EvolutionaryTraceiP60519.

Family and domain databases

InterProiIPR004241. Atg8-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10969. PTHR10969. 1 hit.
PfamiPF02991. Atg8. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28."
    Sagiv Y., Legesse-Miller A., Porat A., Elazar Z.
    EMBO J. 19:1494-1504(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-47; 55-65; 69-74 AND 107-116, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH NSF AND GOSR1.
    Tissue: Brain.
  2. "Cellular sequences in pestivirus genomes encoding gamma-aminobutyric acid (A) receptor-associated protein and Golgi-associated ATPase enhancer of 16 kilodaltons."
    Becher P., Thiel H.-J., Collins M., Brownlie J., Orlich M.
    J. Virol. 76:13069-13076(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Hypothalamus.
  5. "Structure of GATE-16, membrane transport modulator and mammalian ortholog of autophagocytosis factor Aut7p."
    Paz Y., Elazar Z., Fass D.
    J. Biol. Chem. 275:25445-25450(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS), SUBUNIT.

Entry informationi

Entry nameiGBRL2_BOVIN
AccessioniPrimary (citable) accession number: P60519
Secondary accession number(s): O08765
, Q3ZC59, Q5E9G2, Q9DCP8, Q9UQF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: June 8, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.