Reviewed, UniProtKB/Swiss-Prot P60519 (GBRL2_BOVIN)

Names and origin

Protein names

Recommended name:
    Gamma-aminobutyric acid receptor-associated protein-like 2
Alternative name(s):
    GABA(A) receptor-associated protein-like 2
    Golgi-associated ATPase enhancer of 16 kDa
      Short name=GATE-16
    General protein transport factor p16
    Ganglioside expression factor 2
      Short name=GEF-2
    MAP1 light chain 3-related protein

Gene names

Name:	GABARAPL2
Synonyms:	GEF2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	117 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1. Ref.1
Subunit structure	Interacts with GABRG2, ULK1 and beta-tubulin By similarity. Monomer. Interacts with NSF and GOSR1.
Subcellular location	Golgi apparatus. Ref.1
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR By similarity.
Sequence similarities	Belongs to the MAP1 LC3 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus
PTM	Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	intra-Golgi vesicle-mediated transport Ref.1 Inferred from direct assay. Source: UniProtKB positive regulation of ATPase activity Ref.1 Inferred from direct assay. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi membrane Ref.1 Inferred from direct assay. Source: UniProtKB cytosol Inferred from direct assay. Source: UniProtKB
Molecular function	ATPase binding Ref.1 Inferred from direct assay. Source: UniProtKB SNARE binding Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 117

117

Gamma-aminobutyric acid receptor-associated protein-like 2

PRO_0000212372

Regions

Region

1 – 22

22

Interaction with beta-tubulin By similarity

Region

36 – 68

33

Interaction with GABRG2 Potential

Amino acid modifications

Modified residue

10

1

Phosphoserine By similarity

Modified residue

39

1

Phosphoserine By similarity

Secondary structure

1

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117

Helix Strand Turn

Details...

Helix

4 – 8

5

Helix

11 – 24

14

Beta strand

28 – 35

8

Beta strand

48 – 52

5

Helix

57 – 68

12

Beta strand

77 – 80

4

Helix

91 – 98

8

Beta strand

105 – 110

6

Sequences

	Sequence		Length	Mass (Da)
	P60519-1 [UniParc]. Last modified March 1, 2004. Version 1. Checksum: 17ACB540FD5B975B	FASTA	117	13,667
	10 20 30 40 50 60 MKWMFKEDHS LEHRCVESAK IRAKYPDRVP VIVEKVSGSQ IVDIDKRKYL VPSDITVAQF 70 80 90 100 110 MWIIRKRIQL PSEKAIFLFV DKTVPQSSLT MGQLYEKEKD EDGFLYVAYS GENTFGF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28." Sagiv Y., Legesse-Miller A., Porat A., Elazar Z. EMBO J. 19:1494-1504(2000) [PubMed: 10747018] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-47; 55-65; 69-74 AND 107-116, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH NSF AND GOSR1. Tissue: Brain.
[2]	"Cellular sequences in pestivirus genomes encoding gamma-aminobutyric acid (A) receptor-associated protein and Golgi-associated ATPase enhancer of 16 kilodaltons." Becher P., Thiel H.-J., Collins M., Brownlie J., Orlich M. J. Virol. 76:13069-13076(2002) [PubMed: 12438634] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[3]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Hypothalamus.
[5]	"Structure of GATE-16, membrane transport modulator and mammalian ortholog of autophagocytosis factor Aut7p." Paz Y., Elazar Z., Fass D. J. Biol. Chem. 275:25445-25450(2000) [PubMed: 10856287] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS), SUBUNIT.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF020262 mRNA. Translation: AAD20720.1.
AY117147 mRNA. Translation: AAM77036.1.
BT020958 mRNA. Translation: AAX08975.1.
BC102902 mRNA. Translation: AAI02903.2.

IPI

IPI00715504.

RefSeq

NP_777100.1.

UniGene

Bt.41007

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EO6	X-ray	1.80	A/B	1-117	[»]

ModBase

Search...

Genome annotation databases

Ensembl

ENSBTAG00000006550. Bos taurus. [Contig view]

GeneID

282531.

KEGG

bta:282531.

Phylogenomic databases

HOVERGEN

P60519.

Family and domain databases

InterPro

IPR004241. MAP1_LC3.
[Graphical view]

PANTHER

PTHR10969. MAP1_LC3. 1 hit.

Pfam

PF02991. MAP1_LC3. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

GBRL2_BOVIN

Accession

Primary (citable) accession number: P60519
Secondary accession number(s): O08765 , Q3ZC59, Q5E9G2, Q9DCP8, Q9UQF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	March 1, 2004
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)