Reviewed, UniProtKB/Swiss-Prot P60517 (GBRAP_RAT)

Names and origin

Protein names

Recommended name:
    Gamma-aminobutyric acid receptor-associated protein
Alternative name(s):
    GABA(A) receptor-associated protein

Gene names

Name:

Gabarap

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	117 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton.
Subunit structure	Interacts with GABRG2, TUBA1 and ULK1 By similarity. Interacts with beta-tubulin, GPHN and NSF.
Subcellular location	Intracytoplasmic membrane. Cytoplasm › cytoskeleton. Golgi apparatus membrane. Note= Largely associated with intracellular membrane structures including the Golgi apparatus and post-synaptic cisternae. Colocalizes with microtubules.
Tissue specificity	Can be found in both somatodendritic and axonal compartment of neurons.
Sequence similarities	Belongs to the MAP1 LC3 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Cytoskeleton Golgi apparatus Membrane Microtubule
Technical term	3D-structure
Gene Ontology (GO)
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Nsf	Q9QUL6	1	EBI-925812,EBI-925794

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 117

117

Gamma-aminobutyric acid receptor-associated protein

Regions

Region

1 – 22

22

Interaction with beta-tubulin

Region

36 – 117

82

Interaction with GPHN

Region

36 – 68

33

Interaction with GABRG2 Potential

Secondary structure

1

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117

Helix Strand Turn

Details...

Helix

4 – 8

5

Helix

11 – 24

14

Beta strand

28 – 35

8

Beta strand

48 – 52

5

Helix

57 – 68

12

Beta strand

77 – 82

6

Helix

91 – 98

8

Beta strand

105 – 112

8

Sequences

	Sequence		Length	Mass (Da)
	P60517-1 [UniParc]. Last modified March 1, 2004. Version 1. Checksum: BC0B84B8A51C1E32	FASTA	117	13,918
	10 20 30 40 50 60 MKFVYKEEHP FEKRRSEGEK IRKKYPDRVP VIVEKAPKAR IGDLDKKKYL VPSDLTVGQF 70 80 90 100 110 YFLIRKRIHL RAEDALFFFV NNVIPPTSAT MGQLYQEHHE EDFFLYIAYS DESVYGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Binding of the GABA(A) receptor-associated protein (GABARAP) to microtubules and microfilaments suggests involvement of the cytoskeleton in GABARAPGABA(A) receptor interaction." Wang H., Olsen R.W. J. Neurochem. 75:644-655(2000) [PubMed: 10899939] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INTERACTION WITH BETA-TUBULIN.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
[3]	"The gamma-aminobutyric acid type A receptor (GABAAR)-associated protein GABARAP interacts with gephyrin but is not involved in receptor anchoring at the synapse." Kneussel M., Haverkamp S., Fuhrmann J.C., Wang H., Waessle H., Olsen R.W., Betz H. Proc. Natl. Acad. Sci. U.S.A. 97:8594-8599(2000) [PubMed: 10900017] [Abstract] Cited for: INTERACTION WITH GPHN.
[4]	"The subcellular distribution of GABARAP and its ability to interact with NSF suggest a role for this protein in the intracellular transport of GABA(A) receptors." Kittler J.T., Rostaing P., Schiavo G., Fritschy J.-M., Olsen R., Triller A., Moss S.J. Mol. Cell. Neurosci. 18:13-25(2001) [PubMed: 11461150] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH NSF.
[5]	"Crystal structure of the GABA(A)-receptor-associated protein, GABARAP." Bavro V.N., Sola M., Bracher A., Kneussel M., Betz H., Weissenhorn W. EMBO Rep. 3:183-189(2002) [PubMed: 11818336] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF161588 mRNA. Translation: AAD47643.1.
BC058441 mRNA. Translation: AAH58441.1.

RefSeq

NP_742033.1.

UniGene

Rn.8411

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KJT	X-ray	2.00	A	1-117	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P60517.

Genome annotation databases

Ensembl

ENSRNOG00000017417. Rattus norvegicus. [Contig view]

GeneID

58974.

KEGG

rno:58974.

Organism-specific databases

RGD

61911. Gabarap.

Phylogenomic databases

HOVERGEN

P60517.

Gene expression databases

ArrayExpress

P60517.

GermOnline

ENSRNOG00000017417. Rattus norvegicus.

Family and domain databases

InterPro

IPR004241. MAP1_LC3.
[Graphical view]

PANTHER

PTHR10969. MAP1_LC3. 1 hit.

Pfam

PF02991. MAP1_LC3. 1 hit.
[Graphical view]

ProDom

P60517.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

Search...

Other Resources

LinkHub

P60517.

ProtoNet

Search...

Entry information

Entry name

GBRAP_RAT

Accession

Primary (citable) accession number: P60517
Secondary accession number(s): Q9QUI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	March 1, 2004
Last modified:	July 22, 2008
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)