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P60517

- GBRAP_RAT

UniProt

P60517 - GBRAP_RAT

Protein

Gamma-aminobutyric acid receptor-associated protein

Gene

Gabarap

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Involved in apoptosis By similarity. May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in autophagy By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei116 – 1172Cleavage; by ATG4By similarity

    GO - Molecular functioni

    1. GABA receptor binding Source: RGD
    2. protein binding Source: RGD

    GO - Biological processi

    1. autophagy Source: UniProtKB-KW
    2. extrinsic apoptotic signaling pathway via death domain receptors Source: UniProtKB
    3. microtubule cytoskeleton organization Source: Ensembl
    4. protein transport Source: RGD

    Keywords - Biological processi

    Apoptosis, Autophagy, Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Gamma-aminobutyric acid receptor-associated protein
    Alternative name(s):
    GABA(A) receptor-associated protein
    Gene namesi
    Name:Gabarap
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 10

    Organism-specific databases

    RGDi61911. Gabarap.

    Subcellular locationi

    Endomembrane system. Cytoplasmcytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicleautophagosome By similarity
    Note: Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules. Localizes also to discrete punctae along the ciliary axoneme By similarity.By similarity

    GO - Cellular componenti

    1. actin cytoskeleton Source: Ensembl
    2. autophagic vacuole Source: UniProtKB
    3. autophagic vacuole membrane Source: Ensembl
    4. axoneme Source: UniProtKB
    5. cell body Source: RGD
    6. cytoplasmic vesicle Source: UniProtKB-KW
    7. Golgi membrane Source: UniProtKB-SubCell
    8. lysosome Source: Ensembl
    9. microtubule Source: UniProtKB-KW
    10. microtubule associated complex Source: Ensembl
    11. perinuclear region of cytoplasm Source: RGD
    12. plasma membrane Source: Ensembl
    13. smooth endoplasmic reticulum Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Golgi apparatus, Membrane, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 116116Gamma-aminobutyric acid receptor-associated proteinPRO_0000212366Add
    BLAST
    Propeptidei117 – 1171Removed in mature formBy similarityPRO_0000423068

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi116 – 1161Phosphatidylethanolamine amidated glycineBy similarity

    Post-translational modificationi

    The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II By similarity.By similarity

    Keywords - PTMi

    Lipoprotein

    Proteomic databases

    PRIDEiP60517.

    Expressioni

    Tissue specificityi

    Can be found in both somatodendritic and axonal compartment of neurons.1 Publication

    Gene expression databases

    GenevestigatoriP60517.

    Interactioni

    Subunit structurei

    Interacts with ATG3, ATG7, CALR, DDX47, GABRG2, TUBA1 and ULK1 By similarity. Interacts with beta-tubulin, GPHN and NSF. Interacts with TP53INP1 and TP53INP2 By similarity. Interacts with TBC1D25 By similarity. Directly interacts with SQSTM1 By similarity. Interacts with MAPK15 By similarity. Interacts with TECPR2 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi248705. 5 interactions.
    IntActiP60517. 1 interaction.
    STRINGi10116.ENSRNOP00000023724.

    Structurei

    Secondary structure

    1
    117
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 85
    Helixi11 – 2414
    Beta strandi28 – 358
    Beta strandi48 – 525
    Helixi57 – 6812
    Beta strandi77 – 826
    Helixi91 – 988
    Beta strandi105 – 1128

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1KJTX-ray2.00A1-117[»]
    ProteinModelPortaliP60517.
    SMRiP60517. Positions 1-116.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP60517.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 2222Interaction with beta-tubulinAdd
    BLAST
    Regioni36 – 11782Interaction with GPHNAdd
    BLAST
    Regioni36 – 6833Interaction with GABRG2Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ATG8 family.Curated

    Phylogenomic databases

    eggNOGiNOG263746.
    GeneTreeiENSGT00390000012937.
    HOGENOMiHOG000232034.
    HOVERGENiHBG051706.
    InParanoidiP60517.
    KOiK08341.
    OMAiTTMGQLY.
    OrthoDBiEOG70KGRK.
    PhylomeDBiP60517.
    TreeFamiTF314556.

    Family and domain databases

    InterProiIPR004241. Atg8_like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PANTHERiPTHR10969. PTHR10969. 1 hit.
    PfamiPF02991. Atg8. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P60517-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKFVYKEEHP FEKRRSEGEK IRKKYPDRVP VIVEKAPKAR IGDLDKKKYL    50
    VPSDLTVGQF YFLIRKRIHL RAEDALFFFV NNVIPPTSAT MGQLYQEHHE 100
    EDFFLYIAYS DESVYGL 117
    Length:117
    Mass (Da):13,918
    Last modified:March 1, 2004 - v1
    Checksum:iBC0B84B8A51C1E32
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF161588 mRNA. Translation: AAD47643.1.
    BC058441 mRNA. Translation: AAH58441.1.
    RefSeqiNP_742033.1. NM_172036.3.
    UniGeneiRn.8411.

    Genome annotation databases

    EnsembliENSRNOT00000023724; ENSRNOP00000023724; ENSRNOG00000017417.
    GeneIDi58974.
    KEGGirno:58974.
    UCSCiRGD:61911. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF161588 mRNA. Translation: AAD47643.1 .
    BC058441 mRNA. Translation: AAH58441.1 .
    RefSeqi NP_742033.1. NM_172036.3.
    UniGenei Rn.8411.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1KJT X-ray 2.00 A 1-117 [» ]
    ProteinModelPortali P60517.
    SMRi P60517. Positions 1-116.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 248705. 5 interactions.
    IntActi P60517. 1 interaction.
    STRINGi 10116.ENSRNOP00000023724.

    Proteomic databases

    PRIDEi P60517.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000023724 ; ENSRNOP00000023724 ; ENSRNOG00000017417 .
    GeneIDi 58974.
    KEGGi rno:58974.
    UCSCi RGD:61911. rat.

    Organism-specific databases

    CTDi 11337.
    RGDi 61911. Gabarap.

    Phylogenomic databases

    eggNOGi NOG263746.
    GeneTreei ENSGT00390000012937.
    HOGENOMi HOG000232034.
    HOVERGENi HBG051706.
    InParanoidi P60517.
    KOi K08341.
    OMAi TTMGQLY.
    OrthoDBi EOG70KGRK.
    PhylomeDBi P60517.
    TreeFami TF314556.

    Miscellaneous databases

    EvolutionaryTracei P60517.
    NextBioi 611644.
    PROi P60517.

    Gene expression databases

    Genevestigatori P60517.

    Family and domain databases

    InterProi IPR004241. Atg8_like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    PANTHERi PTHR10969. PTHR10969. 1 hit.
    Pfami PF02991. Atg8. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Binding of the GABA(A) receptor-associated protein (GABARAP) to microtubules and microfilaments suggests involvement of the cytoskeleton in GABARAPGABA(A) receptor interaction."
      Wang H., Olsen R.W.
      J. Neurochem. 75:644-655(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INTERACTION WITH BETA-TUBULIN.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pituitary.
    3. "The gamma-aminobutyric acid type A receptor (GABAAR)-associated protein GABARAP interacts with gephyrin but is not involved in receptor anchoring at the synapse."
      Kneussel M., Haverkamp S., Fuhrmann J.C., Wang H., Waessle H., Olsen R.W., Betz H.
      Proc. Natl. Acad. Sci. U.S.A. 97:8594-8599(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GPHN.
    4. "The subcellular distribution of GABARAP and its ability to interact with NSF suggest a role for this protein in the intracellular transport of GABA(A) receptors."
      Kittler J.T., Rostaing P., Schiavo G., Fritschy J.-M., Olsen R., Triller A., Moss S.J.
      Mol. Cell. Neurosci. 18:13-25(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH NSF.
    5. "Crystal structure of the GABA(A)-receptor-associated protein, GABARAP."
      Bavro V.N., Sola M., Bracher A., Kneussel M., Betz H., Weissenhorn W.
      EMBO Rep. 3:183-189(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

    Entry informationi

    Entry nameiGBRAP_RAT
    AccessioniPrimary (citable) accession number: P60517
    Secondary accession number(s): Q9QUI7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2004
    Last sequence update: March 1, 2004
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3