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Protein

Gamma-aminobutyric acid receptor-associated protein

Gene

Gabarap

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in apoptosis (By similarity). May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in autophagy (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei116 – 1172Cleavage; by ATG4By similarity

GO - Molecular functioni

  1. GABA receptor binding Source: RGD
  2. microtubule binding Source: GO_Central

GO - Biological processi

  1. autophagic vacuole assembly Source: GO_Central
  2. extrinsic apoptotic signaling pathway via death domain receptors Source: UniProtKB
  3. membrane fusion Source: GO_Central
  4. microtubule cytoskeleton organization Source: Ensembl
  5. mitochondrion degradation Source: GO_Central
  6. nucleophagy Source: GO_Central
  7. protein transport Source: RGD
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Autophagy, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor-associated protein
Alternative name(s):
GABA(A) receptor-associated protein
Gene namesi
Name:Gabarap
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi61911. Gabarap.

Subcellular locationi

Endomembrane system. Cytoplasmcytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicleautophagosome By similarity
Note: Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules. Localizes also to discrete punctae along the ciliary axoneme (By similarity).By similarity

GO - Cellular componenti

  1. actin cytoskeleton Source: Ensembl
  2. autophagic vacuole Source: UniProtKB
  3. autophagic vacuole membrane Source: GO_Central
  4. axoneme Source: UniProtKB
  5. cell body Source: RGD
  6. cytoplasmic vesicle Source: UniProtKB-KW
  7. cytosol Source: GO_Central
  8. extrinsic component of membrane Source: GO_Central
  9. Golgi membrane Source: UniProtKB-SubCell
  10. lysosome Source: Ensembl
  11. microtubule Source: UniProtKB-KW
  12. microtubule associated complex Source: Ensembl
  13. perinuclear region of cytoplasm Source: RGD
  14. plasma membrane Source: Ensembl
  15. pre-autophagosomal structure Source: GO_Central
  16. smooth endoplasmic reticulum Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Golgi apparatus, Membrane, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 116116Gamma-aminobutyric acid receptor-associated proteinPRO_0000212366Add
BLAST
Propeptidei117 – 1171Removed in mature formBy similarityPRO_0000423068

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi116 – 1161Phosphatidylethanolamine amidated glycineBy similarity

Post-translational modificationi

The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II (By similarity).By similarity

Keywords - PTMi

Lipoprotein

Proteomic databases

PRIDEiP60517.

Expressioni

Tissue specificityi

Can be found in both somatodendritic and axonal compartment of neurons.1 Publication

Gene expression databases

GenevestigatoriP60517.

Interactioni

Subunit structurei

Interacts with ATG3, ATG7, CALR, DDX47, GABRG2, TUBA1 and ULK1 (By similarity). Interacts with beta-tubulin, GPHN and NSF. Interacts with TP53INP1 and TP53INP2 (By similarity). Interacts with TBC1D25 (By similarity). Directly interacts with SQSTM1 (By similarity). Interacts with MAPK15 (By similarity). Interacts with TECPR2 (By similarity). Interacts with TBC1D5 (By similarity).By similarity

Protein-protein interaction databases

BioGridi248705. 5 interactions.
IntActiP60517. 1 interaction.
STRINGi10116.ENSRNOP00000023724.

Structurei

Secondary structure

1
117
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 85Combined sources
Helixi11 – 2414Combined sources
Beta strandi28 – 358Combined sources
Beta strandi48 – 525Combined sources
Helixi57 – 6812Combined sources
Beta strandi77 – 826Combined sources
Helixi91 – 988Combined sources
Beta strandi105 – 1128Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KJTX-ray2.00A1-117[»]
SMRiP60517. Positions 1-116.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60517.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2222Interaction with beta-tubulinAdd
BLAST
Regioni36 – 11782Interaction with GPHNAdd
BLAST
Regioni36 – 6833Interaction with GABRG2Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the ATG8 family.Curated

Phylogenomic databases

eggNOGiNOG263746.
GeneTreeiENSGT00390000012937.
HOGENOMiHOG000232034.
HOVERGENiHBG051706.
InParanoidiP60517.
KOiK08341.
OMAiVYGSSQR.
OrthoDBiEOG70KGRK.
PhylomeDBiP60517.
TreeFamiTF314556.

Family and domain databases

InterProiIPR004241. Atg8_like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10969. PTHR10969. 1 hit.
PfamiPF02991. Atg8. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60517-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFVYKEEHP FEKRRSEGEK IRKKYPDRVP VIVEKAPKAR IGDLDKKKYL
60 70 80 90 100
VPSDLTVGQF YFLIRKRIHL RAEDALFFFV NNVIPPTSAT MGQLYQEHHE
110
EDFFLYIAYS DESVYGL
Length:117
Mass (Da):13,918
Last modified:February 29, 2004 - v1
Checksum:iBC0B84B8A51C1E32
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF161588 mRNA. Translation: AAD47643.1.
BC058441 mRNA. Translation: AAH58441.1.
RefSeqiNP_742033.1. NM_172036.3.
UniGeneiRn.8411.

Genome annotation databases

EnsembliENSRNOT00000023724; ENSRNOP00000023724; ENSRNOG00000017417.
GeneIDi58974.
KEGGirno:58974.
UCSCiRGD:61911. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF161588 mRNA. Translation: AAD47643.1.
BC058441 mRNA. Translation: AAH58441.1.
RefSeqiNP_742033.1. NM_172036.3.
UniGeneiRn.8411.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KJTX-ray2.00A1-117[»]
SMRiP60517. Positions 1-116.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248705. 5 interactions.
IntActiP60517. 1 interaction.
STRINGi10116.ENSRNOP00000023724.

Proteomic databases

PRIDEiP60517.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023724; ENSRNOP00000023724; ENSRNOG00000017417.
GeneIDi58974.
KEGGirno:58974.
UCSCiRGD:61911. rat.

Organism-specific databases

CTDi11337.
RGDi61911. Gabarap.

Phylogenomic databases

eggNOGiNOG263746.
GeneTreeiENSGT00390000012937.
HOGENOMiHOG000232034.
HOVERGENiHBG051706.
InParanoidiP60517.
KOiK08341.
OMAiVYGSSQR.
OrthoDBiEOG70KGRK.
PhylomeDBiP60517.
TreeFamiTF314556.

Miscellaneous databases

EvolutionaryTraceiP60517.
NextBioi611644.
PROiP60517.

Gene expression databases

GenevestigatoriP60517.

Family and domain databases

InterProiIPR004241. Atg8_like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10969. PTHR10969. 1 hit.
PfamiPF02991. Atg8. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Binding of the GABA(A) receptor-associated protein (GABARAP) to microtubules and microfilaments suggests involvement of the cytoskeleton in GABARAPGABA(A) receptor interaction."
    Wang H., Olsen R.W.
    J. Neurochem. 75:644-655(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INTERACTION WITH BETA-TUBULIN.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.
  3. "The gamma-aminobutyric acid type A receptor (GABAAR)-associated protein GABARAP interacts with gephyrin but is not involved in receptor anchoring at the synapse."
    Kneussel M., Haverkamp S., Fuhrmann J.C., Wang H., Waessle H., Olsen R.W., Betz H.
    Proc. Natl. Acad. Sci. U.S.A. 97:8594-8599(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GPHN.
  4. "The subcellular distribution of GABARAP and its ability to interact with NSF suggest a role for this protein in the intracellular transport of GABA(A) receptors."
    Kittler J.T., Rostaing P., Schiavo G., Fritschy J.-M., Olsen R., Triller A., Moss S.J.
    Mol. Cell. Neurosci. 18:13-25(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH NSF.
  5. "Crystal structure of the GABA(A)-receptor-associated protein, GABARAP."
    Bavro V.N., Sola M., Bracher A., Kneussel M., Betz H., Weissenhorn W.
    EMBO Rep. 3:183-189(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

Entry informationi

Entry nameiGBRAP_RAT
AccessioniPrimary (citable) accession number: P60517
Secondary accession number(s): Q9QUI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 29, 2004
Last sequence update: February 29, 2004
Last modified: March 31, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.