Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P60517

- GBRAP_RAT

UniProt

P60517 - GBRAP_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Gamma-aminobutyric acid receptor-associated protein

Gene

Gabarap

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in apoptosis (By similarity). May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in autophagy (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei116 – 1172Cleavage; by ATG4By similarity

GO - Molecular functioni

  1. GABA receptor binding Source: RGD

GO - Biological processi

  1. autophagy Source: UniProtKB-KW
  2. extrinsic apoptotic signaling pathway via death domain receptors Source: UniProtKB
  3. microtubule cytoskeleton organization Source: Ensembl
  4. protein transport Source: RGD
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Autophagy, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor-associated protein
Alternative name(s):
GABA(A) receptor-associated protein
Gene namesi
Name:Gabarap
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 10

Organism-specific databases

RGDi61911. Gabarap.

Subcellular locationi

Endomembrane system. Cytoplasmcytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicleautophagosome By similarity
Note: Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules. Localizes also to discrete punctae along the ciliary axoneme (By similarity).By similarity

GO - Cellular componenti

  1. actin cytoskeleton Source: Ensembl
  2. autophagic vacuole Source: UniProtKB
  3. autophagic vacuole membrane Source: Ensembl
  4. axoneme Source: UniProtKB
  5. cell body Source: RGD
  6. cytoplasmic vesicle Source: UniProtKB-KW
  7. Golgi apparatus Source: UniProtKB-KW
  8. lysosome Source: Ensembl
  9. microtubule Source: UniProtKB-KW
  10. microtubule associated complex Source: Ensembl
  11. perinuclear region of cytoplasm Source: RGD
  12. plasma membrane Source: Ensembl
  13. smooth endoplasmic reticulum Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Golgi apparatus, Membrane, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 116116Gamma-aminobutyric acid receptor-associated proteinPRO_0000212366Add
BLAST
Propeptidei117 – 1171Removed in mature formBy similarityPRO_0000423068

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi116 – 1161Phosphatidylethanolamine amidated glycineBy similarity

Post-translational modificationi

The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II (By similarity).By similarity

Keywords - PTMi

Lipoprotein

Proteomic databases

PRIDEiP60517.

Expressioni

Tissue specificityi

Can be found in both somatodendritic and axonal compartment of neurons.1 Publication

Gene expression databases

GenevestigatoriP60517.

Interactioni

Subunit structurei

Interacts with ATG3, ATG7, CALR, DDX47, GABRG2, TUBA1 and ULK1 (By similarity). Interacts with beta-tubulin, GPHN and NSF. Interacts with TP53INP1 and TP53INP2 (By similarity). Interacts with TBC1D25 (By similarity). Directly interacts with SQSTM1 (By similarity). Interacts with MAPK15 (By similarity). Interacts with TECPR2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi248705. 5 interactions.
IntActiP60517. 1 interaction.
STRINGi10116.ENSRNOP00000023724.

Structurei

Secondary structure

1
117
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 85Combined sources
Helixi11 – 2414Combined sources
Beta strandi28 – 358Combined sources
Beta strandi48 – 525Combined sources
Helixi57 – 6812Combined sources
Beta strandi77 – 826Combined sources
Helixi91 – 988Combined sources
Beta strandi105 – 1128Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KJTX-ray2.00A1-117[»]
ProteinModelPortaliP60517.
SMRiP60517. Positions 1-116.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60517.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2222Interaction with beta-tubulinAdd
BLAST
Regioni36 – 11782Interaction with GPHNAdd
BLAST
Regioni36 – 6833Interaction with GABRG2Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the ATG8 family.Curated

Phylogenomic databases

eggNOGiNOG263746.
GeneTreeiENSGT00390000012937.
HOGENOMiHOG000232034.
HOVERGENiHBG051706.
InParanoidiP60517.
KOiK08341.
OMAiTTMGQLY.
OrthoDBiEOG70KGRK.
PhylomeDBiP60517.
TreeFamiTF314556.

Family and domain databases

InterProiIPR004241. Atg8_like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10969. PTHR10969. 1 hit.
PfamiPF02991. Atg8. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60517 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFVYKEEHP FEKRRSEGEK IRKKYPDRVP VIVEKAPKAR IGDLDKKKYL
60 70 80 90 100
VPSDLTVGQF YFLIRKRIHL RAEDALFFFV NNVIPPTSAT MGQLYQEHHE
110
EDFFLYIAYS DESVYGL
Length:117
Mass (Da):13,918
Last modified:March 1, 2004 - v1
Checksum:iBC0B84B8A51C1E32
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF161588 mRNA. Translation: AAD47643.1.
BC058441 mRNA. Translation: AAH58441.1.
RefSeqiNP_742033.1. NM_172036.3.
UniGeneiRn.8411.

Genome annotation databases

EnsembliENSRNOT00000023724; ENSRNOP00000023724; ENSRNOG00000017417.
GeneIDi58974.
KEGGirno:58974.
UCSCiRGD:61911. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF161588 mRNA. Translation: AAD47643.1 .
BC058441 mRNA. Translation: AAH58441.1 .
RefSeqi NP_742033.1. NM_172036.3.
UniGenei Rn.8411.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1KJT X-ray 2.00 A 1-117 [» ]
ProteinModelPortali P60517.
SMRi P60517. Positions 1-116.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 248705. 5 interactions.
IntActi P60517. 1 interaction.
STRINGi 10116.ENSRNOP00000023724.

Proteomic databases

PRIDEi P60517.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000023724 ; ENSRNOP00000023724 ; ENSRNOG00000017417 .
GeneIDi 58974.
KEGGi rno:58974.
UCSCi RGD:61911. rat.

Organism-specific databases

CTDi 11337.
RGDi 61911. Gabarap.

Phylogenomic databases

eggNOGi NOG263746.
GeneTreei ENSGT00390000012937.
HOGENOMi HOG000232034.
HOVERGENi HBG051706.
InParanoidi P60517.
KOi K08341.
OMAi TTMGQLY.
OrthoDBi EOG70KGRK.
PhylomeDBi P60517.
TreeFami TF314556.

Miscellaneous databases

EvolutionaryTracei P60517.
NextBioi 611644.
PROi P60517.

Gene expression databases

Genevestigatori P60517.

Family and domain databases

InterProi IPR004241. Atg8_like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
PANTHERi PTHR10969. PTHR10969. 1 hit.
Pfami PF02991. Atg8. 1 hit.
[Graphical view ]
SUPFAMi SSF54236. SSF54236. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Binding of the GABA(A) receptor-associated protein (GABARAP) to microtubules and microfilaments suggests involvement of the cytoskeleton in GABARAPGABA(A) receptor interaction."
    Wang H., Olsen R.W.
    J. Neurochem. 75:644-655(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INTERACTION WITH BETA-TUBULIN.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.
  3. "The gamma-aminobutyric acid type A receptor (GABAAR)-associated protein GABARAP interacts with gephyrin but is not involved in receptor anchoring at the synapse."
    Kneussel M., Haverkamp S., Fuhrmann J.C., Wang H., Waessle H., Olsen R.W., Betz H.
    Proc. Natl. Acad. Sci. U.S.A. 97:8594-8599(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GPHN.
  4. "The subcellular distribution of GABARAP and its ability to interact with NSF suggest a role for this protein in the intracellular transport of GABA(A) receptors."
    Kittler J.T., Rostaing P., Schiavo G., Fritschy J.-M., Olsen R., Triller A., Moss S.J.
    Mol. Cell. Neurosci. 18:13-25(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH NSF.
  5. "Crystal structure of the GABA(A)-receptor-associated protein, GABARAP."
    Bavro V.N., Sola M., Bracher A., Kneussel M., Betz H., Weissenhorn W.
    EMBO Rep. 3:183-189(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

Entry informationi

Entry nameiGBRAP_RAT
AccessioniPrimary (citable) accession number: P60517
Secondary accession number(s): Q9QUI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: October 29, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3