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P60517 (GBRAP_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Gamma-aminobutyric acid receptor-associated protein
Alternative name(s):
GABA(A) receptor-associated protein
Gene names
Name:Gabarap
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length117 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in apoptosis By similarity. May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in autophagy By similarity. Ref.4

Subunit structure

Interacts with ATG3, ATG7, CALR, DDX47, GABRG2, TUBA1 and ULK1 By similarity. Interacts with beta-tubulin, GPHN and NSF. Interacts with TP53INP1 and TP53INP2 By similarity. Interacts with TBC1D25 By similarity. Directly interacts with SQSTM1 By similarity. Interacts with MAPK15 By similarity. Interacts with TECPR2 By similarity. Ref.1 Ref.3 Ref.4

Subcellular location

Endomembrane system. Cytoplasmcytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicleautophagosome By similarity. Note: Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules. Localizes also to discrete punctae along the ciliary axoneme By similarity. Ref.1 Ref.4

Tissue specificity

Can be found in both somatodendritic and axonal compartment of neurons. Ref.4

Post-translational modification

The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II By similarity.

Sequence similarities

Belongs to the ATG8 family.

Ontologies

Keywords
   Biological processApoptosis
Autophagy
Protein transport
Transport
   Cellular componentCytoplasm
Cytoplasmic vesicle
Cytoskeleton
Golgi apparatus
Membrane
Microtubule
   PTMLipoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processautophagy

Inferred from electronic annotation. Source: UniProtKB-KW

extrinsic apoptotic signaling pathway via death domain receptors

Inferred from sequence or structural similarity. Source: UniProtKB

microtubule cytoskeleton organization

Inferred from electronic annotation. Source: Ensembl

protein transport

Inferred from mutant phenotype PubMed 15601949PubMed 18160640. Source: RGD

   Cellular_componentGolgi membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

actin cytoskeleton

Inferred from electronic annotation. Source: Ensembl

autophagic vacuole

Inferred from sequence or structural similarity. Source: UniProtKB

autophagic vacuole membrane

Inferred from electronic annotation. Source: Ensembl

axoneme

Inferred from sequence or structural similarity. Source: UniProtKB

cell body

Inferred from direct assay PubMed 15601949. Source: RGD

cytoplasmic vesicle

Inferred from electronic annotation. Source: UniProtKB-KW

lysosome

Inferred from electronic annotation. Source: Ensembl

microtubule

Inferred from electronic annotation. Source: UniProtKB-KW

microtubule associated complex

Inferred from electronic annotation. Source: Ensembl

perinuclear region of cytoplasm

Inferred from direct assay PubMed 15601949. Source: RGD

plasma membrane

Inferred from electronic annotation. Source: Ensembl

smooth endoplasmic reticulum

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionGABA receptor binding

Inferred from physical interaction PubMed 15601949. Source: RGD

protein binding

Inferred from physical interaction PubMed 18160640. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 116116Gamma-aminobutyric acid receptor-associated protein
PRO_0000212366
Propeptide1171Removed in mature form By similarity
PRO_0000423068

Regions

Region1 – 2222Interaction with beta-tubulin
Region36 – 11782Interaction with GPHN
Region36 – 6833Interaction with GABRG2 Potential

Sites

Site116 – 1172Cleavage; by ATG4 By similarity

Amino acid modifications

Lipidation1161Phosphatidylethanolamine amidated glycine By similarity

Secondary structure

................. 117
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P60517 [UniParc].

Last modified March 1, 2004. Version 1.
Checksum: BC0B84B8A51C1E32

FASTA11713,918
        10         20         30         40         50         60 
MKFVYKEEHP FEKRRSEGEK IRKKYPDRVP VIVEKAPKAR IGDLDKKKYL VPSDLTVGQF 

        70         80         90        100        110 
YFLIRKRIHL RAEDALFFFV NNVIPPTSAT MGQLYQEHHE EDFFLYIAYS DESVYGL 

« Hide

References

« Hide 'large scale' references
[1]"Binding of the GABA(A) receptor-associated protein (GABARAP) to microtubules and microfilaments suggests involvement of the cytoskeleton in GABARAPGABA(A) receptor interaction."
Wang H., Olsen R.W.
J. Neurochem. 75:644-655(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INTERACTION WITH BETA-TUBULIN.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pituitary.
[3]"The gamma-aminobutyric acid type A receptor (GABAAR)-associated protein GABARAP interacts with gephyrin but is not involved in receptor anchoring at the synapse."
Kneussel M., Haverkamp S., Fuhrmann J.C., Wang H., Waessle H., Olsen R.W., Betz H.
Proc. Natl. Acad. Sci. U.S.A. 97:8594-8599(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GPHN.
[4]"The subcellular distribution of GABARAP and its ability to interact with NSF suggest a role for this protein in the intracellular transport of GABA(A) receptors."
Kittler J.T., Rostaing P., Schiavo G., Fritschy J.-M., Olsen R., Triller A., Moss S.J.
Mol. Cell. Neurosci. 18:13-25(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH NSF.
[5]"Crystal structure of the GABA(A)-receptor-associated protein, GABARAP."
Bavro V.N., Sola M., Bracher A., Kneussel M., Betz H., Weissenhorn W.
EMBO Rep. 3:183-189(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF161588 mRNA. Translation: AAD47643.1.
BC058441 mRNA. Translation: AAH58441.1.
RefSeqNP_742033.1. NM_172036.3.
UniGeneRn.8411.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1KJTX-ray2.00A1-117[»]
ProteinModelPortalP60517.
SMRP60517. Positions 1-116.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid248705. 5 interactions.
IntActP60517. 1 interaction.
STRING10116.ENSRNOP00000023724.

Proteomic databases

PRIDEP60517.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000023724; ENSRNOP00000023724; ENSRNOG00000017417.
GeneID58974.
KEGGrno:58974.
UCSCRGD:61911. rat.

Organism-specific databases

CTD11337.
RGD61911. Gabarap.

Phylogenomic databases

eggNOGNOG263746.
GeneTreeENSGT00390000012937.
HOGENOMHOG000232034.
HOVERGENHBG051706.
InParanoidP60517.
KOK08341.
OMATTMGQLY.
OrthoDBEOG70KGRK.
PhylomeDBP60517.
TreeFamTF314556.

Gene expression databases

GenevestigatorP60517.

Family and domain databases

InterProIPR004241. Atg8_like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERPTHR10969. PTHR10969. 1 hit.
PfamPF02991. Atg8. 1 hit.
[Graphical view]
SUPFAMSSF54236. SSF54236. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP60517.
NextBio611644.
PROP60517.

Entry information

Entry nameGBRAP_RAT
AccessionPrimary (citable) accession number: P60517
Secondary accession number(s): Q9QUI7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: June 11, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references