P60517 (GBRAP_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-aminobutyric acid receptor-associated protein

Alternative name(s):
GABA(A) receptor-associated protein

Gene names

Name:

Gabarap

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	117 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in apoptosis By similarity. May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in autophagy By similarity. Ref.4
Subunit structure	Interacts with CALR, DDX47, GABRG2, TUBA1 and ULK1 By similarity. Interacts with beta-tubulin, GPHN and NSF. Interacts with TP53INP1 and TP53INP2 By similarity. Interacts with TBC1D25 By similarity. Directly interacts with SQSTM1 By similarity. Ref.1 Ref.3 Ref.4
Subcellular location	Endomembrane system. Cytoplasm › cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle › autophagosome By similarity. Note: Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules. Ref.1 Ref.4
Tissue specificity	Can be found in both somatodendritic and axonal compartment of neurons. Ref.4
Sequence similarities	Belongs to the MAP1 LC3 family.

Ontologies

Keywords
Biological process	Apoptosis Autophagy Protein transport Transport
Cellular component	Cytoplasm Cytoplasmic vesicle Cytoskeleton Golgi apparatus Membrane Microtubule
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB autophagy Inferred from electronic annotation. Source: UniProtKB-KW microtubule cytoskeleton organization Inferred from electronic annotation. Source: Compara protein transport Inferred from mutant phenotype PubMed 15601949PubMed 18160640. Source: RGD
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell actin cytoskeleton Inferred from electronic annotation. Source: Compara autophagic vacuole Inferred from sequence or structural similarity. Source: UniProtKB autophagic vacuole membrane Inferred from electronic annotation. Source: Compara cell body Inferred from direct assay PubMed 15601949. Source: RGD cytoplasmic vesicle Inferred from electronic annotation. Source: UniProtKB-KW lysosome Inferred from electronic annotation. Source: Compara microtubule Inferred from electronic annotation. Source: UniProtKB-KW microtubule associated complex Inferred from electronic annotation. Source: Compara perinuclear region of cytoplasm Inferred from direct assay PubMed 15601949. Source: RGD plasma membrane Inferred from electronic annotation. Source: Compara smooth endoplasmic reticulum Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 117

117

Gamma-aminobutyric acid receptor-associated protein

PRO_0000212366

Regions

Region

1 – 22

22

Interaction with beta-tubulin

Region

36 – 117

82

Interaction with GPHN

Region

36 – 68

33

Interaction with GABRG2 Potential

Secondary structure

1

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117

Helix Strand Turn

Details...

Helix

4 – 8

5

Helix

11 – 24

14

Beta strand

28 – 35

8

Beta strand

48 – 52

5

Helix

57 – 68

12

Beta strand

77 – 82

6

Helix

91 – 98

8

Beta strand

105 – 112

8

Sequences

	Sequence		Length	Mass (Da)
	P60517 [UniParc]. Last modified March 1, 2004. Version 1. Checksum: BC0B84B8A51C1E32	FASTA	117	13,918
	10 20 30 40 50 60 MKFVYKEEHP FEKRRSEGEK IRKKYPDRVP VIVEKAPKAR IGDLDKKKYL VPSDLTVGQF 70 80 90 100 110 YFLIRKRIHL RAEDALFFFV NNVIPPTSAT MGQLYQEHHE EDFFLYIAYS DESVYGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Binding of the GABA(A) receptor-associated protein (GABARAP) to microtubules and microfilaments suggests involvement of the cytoskeleton in GABARAPGABA(A) receptor interaction." Wang H., Olsen R.W. J. Neurochem. 75:644-655(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INTERACTION WITH BETA-TUBULIN.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
[3]	"The gamma-aminobutyric acid type A receptor (GABAAR)-associated protein GABARAP interacts with gephyrin but is not involved in receptor anchoring at the synapse." Kneussel M., Haverkamp S., Fuhrmann J.C., Wang H., Waessle H., Olsen R.W., Betz H. Proc. Natl. Acad. Sci. U.S.A. 97:8594-8599(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GPHN.
[4]	"The subcellular distribution of GABARAP and its ability to interact with NSF suggest a role for this protein in the intracellular transport of GABA(A) receptors." Kittler J.T., Rostaing P., Schiavo G., Fritschy J.-M., Olsen R., Triller A., Moss S.J. Mol. Cell. Neurosci. 18:13-25(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH NSF.
[5]	"Crystal structure of the GABA(A)-receptor-associated protein, GABARAP." Bavro V.N., Sola M., Bracher A., Kneussel M., Betz H., Weissenhorn W. EMBO Rep. 3:183-189(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF161588 mRNA. Translation: AAD47643.1.
BC058441 mRNA. Translation: AAH58441.1.

IPI

IPI00210522.

RefSeq

NP_742033.1. NM_172036.3.

UniGene

Rn.8411.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KJT	X-ray	2.00	A	1-117	[»]

ProteinModelPortal

P60517.

ModBase

Search...

Protein-protein interaction databases

IntAct

P60517. 1 interaction.

STRING

10116.ENSRNOP00000023724.

Proteomic databases

PRIDE

P60517.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000023724; ENSRNOP00000023724; ENSRNOG00000017417.

GeneID

58974.

KEGG

rno:58974.

UCSC

RGD:61911. rat.

Organism-specific databases

CTD

11337.

RGD

61911. Gabarap.

Phylogenomic databases

eggNOG

NOG263746.

GeneTree

ENSGT00390000012937.

HOGENOM

HOG000232034.

HOVERGEN

HBG051706.

InParanoid

P60517.

KO

K08341.

OMA

TTMGQLY.

OrthoDB

EOG4T4CWW.

Gene expression databases

Genevestigator

P60517.

GermOnline

ENSRNOG00000017417. Rattus norvegicus.

Family and domain databases

InterPro

IPR004241. Atg8_ubiquitin-like.
[Graphical view]

PANTHER

PTHR10969. PTHR10969. 1 hit.

Pfam

PF02991. Atg8. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P60517.

NextBio

611644.

Entry information

Entry name

GBRAP_RAT

Accession

Primary (citable) accession number: P60517
Secondary accession number(s): Q9QUI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	March 1, 2004
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program