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Protein

Delta-conotoxin EVIA

Gene
N/A
Organism
Conus ermineus (Atlantic fish-hunting cone)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Delta-conotoxins bind to site 6 of voltage-gated sodium channels and inhibit the inactivation process. This toxin inhibits sodium channel inactivation in neuronal membranes from amphibians and mammals (Nav1.2a/SCN1A, Nav1.3/SCN3A and Nav1.6/SCN8A) upon binding to receptor site 6.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-conotoxin EVIA
Short name:
Delta-EVIA
OrganismiConus ermineus (Atlantic fish-hunting cone)
Taxonomic identifieri55423 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaHypsogastropodaNeogastropodaConoideaConidaeConus

Organism-specific databases

ConoServeri1561. EVIA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

Dose that produces hyperactivity (ED(50)) is 1.8 pmol/g body mass (native toxin), 1.9 pmol/g body mass (synthetic toxin) and 3.9 pmol/g body mass (P13A mutant).1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi13 – 131P → A: 2-fold decrease of activity. Exists only as a trans isomer. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Peptidei1 – 3232Delta-conotoxin EVIAPRO_0000044868Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi3 ↔ 211 Publication
Modified residuei6 – 614-hydroxyproline1 Publication
Disulfide bondi10 ↔ 251 Publication
Disulfide bondi20 ↔ 291 Publication
Modified residuei32 – 321Leucine amide1 Publication

Keywords - PTMi

Amidation, Disulfide bond, Hydroxylation

Expressioni

Tissue specificityi

Expressed by the venom duct.2 Publications

Structurei

Secondary structure

1
32
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi13 – 164Combined sources
Beta strandi25 – 295Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G1PNMR-A1-32[»]
1G1ZNMR-A1-32[»]
ProteinModelPortaliP60513.
SMRiP60513. Positions 1-32.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60513.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.
The cysteine framework is VI/VII (C-C-CC-C-C).

Sequence similaritiesi

Belongs to the conotoxin O1 superfamily.Curated

Keywords - Domaini

Knottin

Family and domain databases

InterProiIPR012322. Conotoxin_d-typ_CS.
[Graphical view]
PROSITEiPS60005. DELTA_CONOTOXIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P60513-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30 
DDCIKPYGFC SLPILKNGLC CSGACVGVCA DL
Length:32
Mass (Da):3,279
Last modified:March 1, 2004 - v1
Checksum:i96C544273232D62D
GO

Mass spectrometryi

Molecular mass is 3288.3 Da from positions 1 - 32. Determined by ESI. 1 Publication

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G1PNMR-A1-32[»]
1G1ZNMR-A1-32[»]
ProteinModelPortaliP60513.
SMRiP60513. Positions 1-32.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ConoServeri1561. EVIA.

Miscellaneous databases

EvolutionaryTraceiP60513.

Family and domain databases

InterProiIPR012322. Conotoxin_d-typ_CS.
[Graphical view]
PROSITEiPS60005. DELTA_CONOTOXIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCO6A_CONER
AccessioniPrimary (citable) accession number: P60513
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: December 9, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

Does not affect rat skeletal muscle (Nav1.4/SCN4A) and human cardiac muscle (Nav1.5/SCN5A) sodium channels.1 Publication
Exists in two forms, due to cis-trans isomerization at 12-Leu-Pro-13.

Caution

Another delta-conotoxin was named EVIA in 2001, but this toxin was renamed EVIB.Curated

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.