Reviewed, UniProtKB/Swiss-Prot P60511 (LEXA2_XANAC)

Names and origin

Protein names

Recommended name:
    LexA repressor 2
    EC=3.4.21.88

Gene names

Name:	lexA2
Ordered Locus Names:	XAC1739

Organism

Xanthomonas axonopodis pv. citri (Citrus canker) [Complete proteome] [HAMAP]

Taxonomic identifier

92829 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Has been shown to bind to the palindromic sequence 5'-CTG-N(8-12)-C-[TC]-G. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity.
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	LexA repressor 2 HAMAP MF_00015		PRO_0000170106
Regions
	DNA binding	27 – 47	21	H-T-H motif By similarity
Sites
	Active site	133	1	For autocatalytic cleavage activity By similarity
	Active site	170	1	For autocatalytic cleavage activity By similarity
	Site	98 – 99	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P60511-1 [UniParc]. Last modified March 1, 2004. Version 1. Checksum: 30C56E5396EAF946	FASTA	213	23,240
	10 20 30 40 50 60 MDLTDTQQAI LALIAERIDA DGVPPSQTEI ARAFGFKGIR AAQYHLEALE HAGAIRRVPG 70 80 90 100 110 120 QARGIRLAGQ GAQTRTAPVS EVARDDVLRL PVLGRVAAGL PIGADIGSDD FVVLDRVFFS 130 140 150 160 170 180 PSPDYLLKVQ GDSMRDEGIF NGDLIGVHRT RDARSGQIVV ARIDEEITVK LLKIGKDRIR 190 200 210 LLPRNPDYAP IEVLPDQDFA IEGLYCGLLR PNR

References

[1]

"Comparison of the genomes of two Xanthomonas pathogens with differing host specificities." da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R., Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr., Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G., Cannavan F., Cardozo J., Chambergo F., Ciapina L.P. , Cicarelli R.M.B., Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B., Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F., Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T., Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A., Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J., Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M., Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A., Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A., Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M., Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P. Nature 417:459-463(2002) [PubMed: 12024217] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 306.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008923 Genomic DNA. Translation: AAM36606.1.
RefSeq	NP_642070.1.
3D structure databases
HSSP	HSSP built from PDB template 1QAA based on UniProtKB P03033.
ModBase	Search...
Genome annotation databases
GeneID	1155810.
GenomeReviews	Gene locus XAC1739 in contig AE008923_GR.
KEGG	xac:XAC1739.
NMPDR	fig|190486.1.peg.1714.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P60511.
OMA	ITNHINA.
Enzyme and pathway databases
BioCyc	XAXO190486:XAC1739-MON.
BRENDA	3.4.21.88. 289771.
Family and domain databases
HAMAP	MF_00015. [Tree]
InterPro	IPR006199. LexA_DNA_bd. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA_cons-reg. IPR019759. Peptidase_S24_S26_cons-reg. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
TIGRFAMs	TIGR00498. lexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA2_XANAC

Accession

Primary (citable) accession number: P60511
Secondary accession number(s): O86050

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	March 1, 2004
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)