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Protein

Ditrans,polycis-undecaprenyl-diphosphate synthase ((2E,6E)-farnesyl-diphosphate specific)

Gene

uppS

Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with (2E,6E)-farnesyl diphosphate (E,E-FPP) to yield (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E,38E)-undecaprenyl diphosphate (di-trans,octa-cis-UPP). UPP is the precursor of glycosyl carrier lipid in the biosynthesis of bacterial cell wall polysaccharide components such as peptidoglycan and lipopolysaccharide.UniRule annotation

Catalytic activityi

(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate = 8 diphosphate + di-trans,octa-cis-undecaprenyl diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei26 – 261UniRule annotation
Metal bindingi26 – 261MagnesiumUniRule annotation
Binding sitei31 – 311SubstrateUniRule annotation
Binding sitei39 – 391SubstrateUniRule annotation
Binding sitei43 – 431SubstrateUniRule annotation
Active sitei74 – 741Proton acceptorUniRule annotation
Binding sitei75 – 751SubstrateUniRule annotation
Binding sitei77 – 771SubstrateUniRule annotation
Binding sitei194 – 1941SubstrateUniRule annotation
Metal bindingi199 – 1991MagnesiumUniRule annotation
Metal bindingi213 – 2131MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciECOL199310:C0211-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ditrans,polycis-undecaprenyl-diphosphate synthase ((2E,6E)-farnesyl-diphosphate specific)UniRule annotation (EC:2.5.1.31UniRule annotation)
Alternative name(s):
Ditrans,polycis-undecaprenylcistransferaseUniRule annotation
Undecaprenyl diphosphate synthaseUniRule annotation
Short name:
UDSUniRule annotation
Undecaprenyl pyrophosphate synthaseUniRule annotation
Short name:
UPP synthaseUniRule annotation
Gene namesi
Name:uppSUniRule annotation
Ordered Locus Names:c0211
OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Taxonomic identifieri199310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000001410 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 253253Ditrans,polycis-undecaprenyl-diphosphate synthase ((2E,6E)-farnesyl-diphosphate specific)PRO_0000123610Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi199310.c0211.

Structurei

Secondary structure

1
253
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi19 – 257Combined sources
Helixi28 – 347Combined sources
Helixi39 – 5921Combined sources
Beta strandi63 – 697Combined sources
Helixi79 – 10224Combined sources
Beta strandi106 – 1116Combined sources
Helixi118 – 13114Combined sources
Beta strandi138 – 1447Combined sources
Helixi147 – 16317Combined sources
Helixi169 – 1713Combined sources
Helixi174 – 1785Combined sources
Turni182 – 1854Combined sources
Beta strandi191 – 1944Combined sources
Helixi207 – 2093Combined sources
Beta strandi213 – 2164Combined sources
Helixi221 – 2233Combined sources
Helixi226 – 23914Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3SGTX-ray1.85A/B1-253[»]
ProteinModelPortaliP60473.
SMRiP60473. Positions 17-238.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni27 – 304Substrate bindingUniRule annotation
Regioni71 – 733Substrate bindingUniRule annotation
Regioni200 – 2023Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the UPP synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CR3. Bacteria.
COG0020. LUCA.
HOGENOMiHOG000006054.
KOiK00806.
OMAiWAKLKNK.
OrthoDBiEOG68H89T.

Family and domain databases

Gene3Di3.40.1180.10. 1 hit.
HAMAPiMF_01139. ISPT.
InterProiIPR001441. UPP_synth-like.
IPR018520. UPP_synth-like_CS.
[Graphical view]
PANTHERiPTHR10291. PTHR10291. 1 hit.
PfamiPF01255. Prenyltransf. 1 hit.
[Graphical view]
SUPFAMiSSF64005. SSF64005. 1 hit.
TIGRFAMsiTIGR00055. uppS. 1 hit.
PROSITEiPS01066. UPP_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P60473-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMLSATQPLS EKLPAHGCRH VAIIMDGNGR WAKKQGKIRA FGHKAGAKSV
60 70 80 90 100
RRAVSFAANN GIEALTLYAF SSENWNRPAQ EVSALMELFV WALDSEVKSL
110 120 130 140 150
HRHNVRLRII GDTSRFNSRL QERIRKSEAL TAGNTGLTLN IAANYGGRWD
160 170 180 190 200
IVQGVRQLAE KVQQGNLQPD QIDEEMLNQH VCMHELAPVD LVIRTGGEHR
210 220 230 240 250
ISNFLLWQIA YAELYFTDVL WPDFDEQDFE GALNAFANRE RRFGGTEPGD

ETA
Length:253
Mass (Da):28,444
Last modified:February 16, 2004 - v1
Checksum:i73DC9534C14CA7B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA. Translation: AAN78703.1.

Genome annotation databases

EnsemblBacteriaiAAN78703; AAN78703; c0211.
KEGGiecc:c0211.
PATRICi18278487. VBIEscCol75197_0198.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA. Translation: AAN78703.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3SGTX-ray1.85A/B1-253[»]
ProteinModelPortaliP60473.
SMRiP60473. Positions 17-238.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi199310.c0211.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN78703; AAN78703; c0211.
KEGGiecc:c0211.
PATRICi18278487. VBIEscCol75197_0198.

Phylogenomic databases

eggNOGiENOG4105CR3. Bacteria.
COG0020. LUCA.
HOGENOMiHOG000006054.
KOiK00806.
OMAiWAKLKNK.
OrthoDBiEOG68H89T.

Enzyme and pathway databases

BioCyciECOL199310:C0211-MONOMER.

Family and domain databases

Gene3Di3.40.1180.10. 1 hit.
HAMAPiMF_01139. ISPT.
InterProiIPR001441. UPP_synth-like.
IPR018520. UPP_synth-like_CS.
[Graphical view]
PANTHERiPTHR10291. PTHR10291. 1 hit.
PfamiPF01255. Prenyltransf. 1 hit.
[Graphical view]
SUPFAMiSSF64005. SSF64005. 1 hit.
TIGRFAMsiTIGR00055. uppS. 1 hit.
PROSITEiPS01066. UPP_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CFT073 / ATCC 700928 / UPEC.

Entry informationi

Entry nameiUPPS_ECOL6
AccessioniPrimary (citable) accession number: P60473
Secondary accession number(s): P75668, Q47675, Q9R2E4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: February 16, 2004
Last modified: November 11, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.