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Protein

Ditrans,polycis-undecaprenyl-diphosphate synthase ((2E,6E)-farnesyl-diphosphate specific)

Gene

uppS

Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with (2E,6E)-farnesyl diphosphate (E,E-FPP) to yield (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E,38E)-undecaprenyl diphosphate (di-trans,octa-cis-UPP). UPP is the precursor of glycosyl carrier lipid in the biosynthesis of bacterial cell wall polysaccharide components such as peptidoglycan and lipopolysaccharide.UniRule annotation

Catalytic activityi

(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate = 8 diphosphate + di-trans,octa-cis-undecaprenyl diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei26UniRule annotation1
Metal bindingi26MagnesiumUniRule annotation1
Binding sitei31SubstrateUniRule annotation1
Binding sitei39SubstrateUniRule annotation1
Binding sitei43SubstrateUniRule annotation1
Active sitei74Proton acceptorUniRule annotation1
Binding sitei75SubstrateUniRule annotation1
Binding sitei77SubstrateUniRule annotation1
Binding sitei194SubstrateUniRule annotation1
Metal bindingi199MagnesiumUniRule annotation1
Metal bindingi213MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciECOL199310:C0211-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ditrans,polycis-undecaprenyl-diphosphate synthase ((2E,6E)-farnesyl-diphosphate specific)UniRule annotation (EC:2.5.1.31UniRule annotation)
Alternative name(s):
Ditrans,polycis-undecaprenylcistransferaseUniRule annotation
Undecaprenyl diphosphate synthaseUniRule annotation
Short name:
UDSUniRule annotation
Undecaprenyl pyrophosphate synthaseUniRule annotation
Short name:
UPP synthaseUniRule annotation
Gene namesi
Name:uppSUniRule annotation
Ordered Locus Names:c0211
OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Taxonomic identifieri199310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000001410 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001236101 – 253Ditrans,polycis-undecaprenyl-diphosphate synthase ((2E,6E)-farnesyl-diphosphate specific)Add BLAST253

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi199310.c0211.

Structurei

Secondary structure

1253
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi19 – 25Combined sources7
Helixi28 – 34Combined sources7
Helixi39 – 59Combined sources21
Beta strandi63 – 69Combined sources7
Helixi79 – 102Combined sources24
Beta strandi106 – 111Combined sources6
Helixi118 – 131Combined sources14
Beta strandi138 – 144Combined sources7
Helixi147 – 163Combined sources17
Helixi169 – 171Combined sources3
Helixi174 – 178Combined sources5
Turni182 – 185Combined sources4
Beta strandi191 – 194Combined sources4
Helixi207 – 209Combined sources3
Beta strandi213 – 216Combined sources4
Helixi221 – 223Combined sources3
Helixi226 – 239Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SGTX-ray1.85A/B1-253[»]
ProteinModelPortaliP60473.
SMRiP60473.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni27 – 30Substrate bindingUniRule annotation4
Regioni71 – 73Substrate bindingUniRule annotation3
Regioni200 – 202Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the UPP synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CR3. Bacteria.
COG0020. LUCA.
HOGENOMiHOG000006054.
KOiK00806.
OMAiWAKLKNK.

Family and domain databases

CDDicd00475. Cis_IPPS. 1 hit.
Gene3Di3.40.1180.10. 1 hit.
HAMAPiMF_01139. ISPT. 1 hit.
InterProiIPR001441. UPP_synth-like.
IPR018520. UPP_synth-like_CS.
[Graphical view]
PANTHERiPTHR10291. PTHR10291. 1 hit.
PfamiPF01255. Prenyltransf. 1 hit.
[Graphical view]
SUPFAMiSSF64005. SSF64005. 1 hit.
TIGRFAMsiTIGR00055. uppS. 1 hit.
PROSITEiPS01066. UPP_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P60473-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMLSATQPLS EKLPAHGCRH VAIIMDGNGR WAKKQGKIRA FGHKAGAKSV
60 70 80 90 100
RRAVSFAANN GIEALTLYAF SSENWNRPAQ EVSALMELFV WALDSEVKSL
110 120 130 140 150
HRHNVRLRII GDTSRFNSRL QERIRKSEAL TAGNTGLTLN IAANYGGRWD
160 170 180 190 200
IVQGVRQLAE KVQQGNLQPD QIDEEMLNQH VCMHELAPVD LVIRTGGEHR
210 220 230 240 250
ISNFLLWQIA YAELYFTDVL WPDFDEQDFE GALNAFANRE RRFGGTEPGD

ETA
Length:253
Mass (Da):28,444
Last modified:February 16, 2004 - v1
Checksum:i73DC9534C14CA7B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA. Translation: AAN78703.1.

Genome annotation databases

EnsemblBacteriaiAAN78703; AAN78703; c0211.
KEGGiecc:c0211.
PATRICi18278487. VBIEscCol75197_0198.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA. Translation: AAN78703.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SGTX-ray1.85A/B1-253[»]
ProteinModelPortaliP60473.
SMRiP60473.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi199310.c0211.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN78703; AAN78703; c0211.
KEGGiecc:c0211.
PATRICi18278487. VBIEscCol75197_0198.

Phylogenomic databases

eggNOGiENOG4105CR3. Bacteria.
COG0020. LUCA.
HOGENOMiHOG000006054.
KOiK00806.
OMAiWAKLKNK.

Enzyme and pathway databases

BioCyciECOL199310:C0211-MONOMER.

Miscellaneous databases

PROiP60473.

Family and domain databases

CDDicd00475. Cis_IPPS. 1 hit.
Gene3Di3.40.1180.10. 1 hit.
HAMAPiMF_01139. ISPT. 1 hit.
InterProiIPR001441. UPP_synth-like.
IPR018520. UPP_synth-like_CS.
[Graphical view]
PANTHERiPTHR10291. PTHR10291. 1 hit.
PfamiPF01255. Prenyltransf. 1 hit.
[Graphical view]
SUPFAMiSSF64005. SSF64005. 1 hit.
TIGRFAMsiTIGR00055. uppS. 1 hit.
PROSITEiPS01066. UPP_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUPPS_ECOL6
AccessioniPrimary (citable) accession number: P60473
Secondary accession number(s): P75668, Q47675, Q9R2E4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: February 16, 2004
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.