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Protein

Liprin-alpha-3

Gene

Ppfia3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May regulate the disassembly of focal adhesions. May localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-181430. Norepinephrine Neurotransmitter Release Cycle.
R-MMU-210500. Glutamate Neurotransmitter Release Cycle.
R-MMU-264642. Acetylcholine Neurotransmitter Release Cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Liprin-alpha-3
Alternative name(s):
Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-3
Short name:
PTPRF-interacting protein alpha-3
Gene namesi
Name:Ppfia3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1924037. Ppfia3.

Subcellular locationi

  • Cytoplasm By similarity
  • Cell surface By similarity

  • Note: Colocalizes with PTPRF at the cell surface.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11941194Liprin-alpha-3PRO_0000191030Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei17 – 171PhosphoserineCombined sources
Modified residuei142 – 1421PhosphoserineBy similarity
Modified residuei207 – 2071PhosphoserineBy similarity
Modified residuei508 – 5081PhosphoserineCombined sources
Modified residuei645 – 6451PhosphoserineCombined sources
Modified residuei714 – 7141PhosphothreonineCombined sources
Modified residuei792 – 7921PhosphothreonineBy similarity
Modified residuei794 – 7941PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP60469.
MaxQBiP60469.
PRIDEiP60469.

PTM databases

iPTMnetiP60469.
PhosphoSiteiP60469.

Expressioni

Gene expression databases

BgeeiP60469.

Interactioni

Subunit structurei

Forms homodimers and heterodimers with liprins-alpha and liprins-beta. Interacts with the second PTPase domain of PTPRD, PTPRF and PTPRS. Binds RIMS1, RIMS2, RIMS3 and RIMS4 (By similarity).By similarity

Protein-protein interaction databases

BioGridi218314. 4 interactions.
IntActiP60469. 3 interactions.

Structurei

Secondary structure

1
1194
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi719 – 73113Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4UWXX-ray1.65C/D718-738[»]
ProteinModelPortaliP60469.
SMRiP60469. Positions 812-1115.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini838 – 90467SAM 1PROSITE-ProRule annotationAdd
BLAST
Domaini953 – 101765SAM 2PROSITE-ProRule annotationAdd
BLAST
Domaini1041 – 111070SAM 3PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili26 – 133108Sequence analysisAdd
BLAST
Coiled coili171 – 501331Sequence analysisAdd
BLAST
Coiled coili596 – 64449Sequence analysisAdd
BLAST
Coiled coili1014 – 104027Sequence analysisAdd
BLAST

Domaini

The N-terminal coiled coil regions mediate homodimerization preferentially and heterodimerization type alpha/alpha. The C-terminal, non-coiled coil regions mediate heterodimerization type alpha/beta and interaction with PTPRD, PTPRF and PTPRS (By similarity).By similarity

Sequence similaritiesi

Belongs to the liprin family. Liprin-alpha subfamily.Curated
Contains 3 SAM (sterile alpha motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0249. Eukaryota.
ENOG410XP8Z. LUCA.
GeneTreeiENSGT00760000119138.
HOGENOMiHOG000017513.
HOVERGENiHBG052330.
InParanoidiP60469.
OMAiSKRQEWE.
OrthoDBiEOG7T7GS7.
TreeFamiTF314207.

Family and domain databases

Gene3Di1.10.150.50. 2 hits.
InterProiIPR029515. Liprin.
IPR030440. PPFIA3.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PANTHERiPTHR12587. PTHR12587. 2 hits.
PTHR12587:SF4. PTHR12587:SF4. 2 hits.
PfamiPF00536. SAM_1. 2 hits.
PF07647. SAM_2. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 3 hits.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 3 hits.
PROSITEiPS50105. SAM_DOMAIN. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P60469-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMCEVMPTIS EDGRRGSALG PDEAGGELER LMVTMLTERE RLLETLREAQ
60 70 80 90 100
DGLATAQLRL RELGHEKDSL QRQLSIALPQ EFAALTKELN LCREQLLERE
110 120 130 140 150
EEIAELKAER NNTRLLLEHL ECLVSRHERS LRMTVVKRQA QSPGGVSSEV
160 170 180 190 200
EVLKALKSLF EHHKALDEKV RERLRMALER VAVLEEELEL SNQEALNLRD
210 220 230 240 250
QLSRRRSGLE EPGKDGDGQT LANGLGPVGE SNRRTAELEE ALERQRAEVC
260 270 280 290 300
QLRERLAVLC RQMSQLEEEL GTAHRELGKA EEANSKLQRD LKEALAQRED
310 320 330 340 350
MEERITTLEK RYLSAQREAT SLHDANDKLE NELASKESLY RQSEEKSRQL
360 370 380 390 400
AEWLDDAKQK LQQTLQKAET LPEIEAQLAQ RVAALNKAEE RHGNFEERLR
410 420 430 440 450
QLEAQLEEKN QELQRARQRE KMNDDHNKRL SETVDKLLSE SNERLQLHLK
460 470 480 490 500
ERMGALEEKN SLSEEIANMK KLQDELLLNK EQLLAEMERM QMEIDQLRGR
510 520 530 540 550
PPSSYSRSLP GSALELRYSQ APTLPSGAPL DPYGAGSGRA GKRGRWSGAK
560 570 580 590 600
DESSKDWDRS APAGSIPPPF PGELDGSDEE EAEGMFGAEL LSPSGQADVQ
610 620 630 640 650
TLAIMLQEQL EAINKEIKLI QEEKETTEQR AEELESRVSS SGLDSLGRYR
660 670 680 690 700
SSCSLPPSLT TSTLASPSPP SSGHSTPRLA PPSPAREGTD KTNHVSKEEA
710 720 730 740 750
GVPRGEGPAV PGDTPPPTPR SARLERMAQA LALQAGSPED GAPPRGSEST
760 770 780 790 800
PDSLHKAPKR KSIKSSIGRL FGKKEKGRMG PPGRESVSLA GTPSDETLAT
810 820 830 840 850
DPLGLAKLTG PGDKDRRNKR KHELLEEACR QGLPFAAWDG PTVVSWLELW
860 870 880 890 900
VGMPAWYVAA CRANVKSGAI MANLSDTEIQ REIGISNPLH RLKLRLAIQE
910 920 930 940 950
MVSLTSPSAP ASSRTPTGNV WMTHEEMESL TAATKPETKE ISWEQILAYG
960 970 980 990 1000
DMNHEWVGND WLPSLGLPQY RSYFMESLVD ARMLDHLNKK ELRGQLKMVD
1010 1020 1030 1040 1050
SFHRVSLHYG IMCLKRLNYD RKDLERRREE SQTQIRDVMV WSNERVMGWV
1060 1070 1080 1090 1100
SGLGLKEFAT NLTESGVHGA LLALDETFDY SDLALLLQIP TQNAQARQLL
1110 1120 1130 1140 1150
EKEFSNLISL GTDRRLDEDS AKSFSRSPSW RKMFREKDLR GVTPDSAEML
1160 1170 1180 1190
PPNFRSAAAG ALGSPGLPLR KLQPEGQTSG SSRADGVSVR TYSC
Length:1,194
Mass (Da):133,426
Last modified:October 14, 2015 - v2
Checksum:iCA6386D6281470FA
GO
Isoform 2 (identifier: P60469-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     178-328: Missing.

Show »
Length:1,043
Mass (Da):116,280
Checksum:i226331BC61954E31
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei178 – 328151Missing in isoform 2. 1 PublicationVSP_057923Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU568871 mRNA. Translation: ACE63189.1.
AC150897 Genomic DNA. No translation available.
AC151602 Genomic DNA. No translation available.
BC058404 mRNA. Translation: AAH58404.1.
CCDSiCCDS52246.1. [P60469-1]
RefSeqiNP_084017.2. NM_029741.2. [P60469-1]
XP_006541336.1. XM_006541273.1. [P60469-1]
XP_011249214.1. XM_011250912.1. [P60469-1]
XP_011249215.1. XM_011250913.1. [P60469-1]
UniGeneiMm.277235.

Genome annotation databases

EnsembliENSMUST00000003961; ENSMUSP00000003961; ENSMUSG00000003863. [P60469-1]
GeneIDi76787.
KEGGimmu:76787.
UCSCiuc012fkh.1. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU568871 mRNA. Translation: ACE63189.1.
AC150897 Genomic DNA. No translation available.
AC151602 Genomic DNA. No translation available.
BC058404 mRNA. Translation: AAH58404.1.
CCDSiCCDS52246.1. [P60469-1]
RefSeqiNP_084017.2. NM_029741.2. [P60469-1]
XP_006541336.1. XM_006541273.1. [P60469-1]
XP_011249214.1. XM_011250912.1. [P60469-1]
XP_011249215.1. XM_011250913.1. [P60469-1]
UniGeneiMm.277235.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4UWXX-ray1.65C/D718-738[»]
ProteinModelPortaliP60469.
SMRiP60469. Positions 812-1115.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218314. 4 interactions.
IntActiP60469. 3 interactions.

PTM databases

iPTMnetiP60469.
PhosphoSiteiP60469.

Proteomic databases

EPDiP60469.
MaxQBiP60469.
PRIDEiP60469.

Protocols and materials databases

DNASUi76787.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003961; ENSMUSP00000003961; ENSMUSG00000003863. [P60469-1]
GeneIDi76787.
KEGGimmu:76787.
UCSCiuc012fkh.1. mouse.

Organism-specific databases

CTDi8541.
MGIiMGI:1924037. Ppfia3.

Phylogenomic databases

eggNOGiKOG0249. Eukaryota.
ENOG410XP8Z. LUCA.
GeneTreeiENSGT00760000119138.
HOGENOMiHOG000017513.
HOVERGENiHBG052330.
InParanoidiP60469.
OMAiSKRQEWE.
OrthoDBiEOG7T7GS7.
TreeFamiTF314207.

Enzyme and pathway databases

ReactomeiR-MMU-181430. Norepinephrine Neurotransmitter Release Cycle.
R-MMU-210500. Glutamate Neurotransmitter Release Cycle.
R-MMU-264642. Acetylcholine Neurotransmitter Release Cycle.

Miscellaneous databases

ChiTaRSiPpfia3. mouse.
PROiP60469.
SOURCEiSearch...

Gene expression databases

BgeeiP60469.

Family and domain databases

Gene3Di1.10.150.50. 2 hits.
InterProiIPR029515. Liprin.
IPR030440. PPFIA3.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PANTHERiPTHR12587. PTHR12587. 2 hits.
PTHR12587:SF4. PTHR12587:SF4. 2 hits.
PfamiPF00536. SAM_1. 2 hits.
PF07647. SAM_2. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 3 hits.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 3 hits.
PROSITEiPS50105. SAM_DOMAIN. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The mouse and human Liprin-alpha family of scaffolding proteins: genomic organization, expression profiling and regulation by alternative splicing."
    Zurner M., Schoch S.
    Genomics 93:243-253(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: C57BL/6JImported.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Brain.
  4. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-645, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  5. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
    Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
    Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain cortex.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17; SER-508; SER-645 AND THR-714, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiLIPA3_MOUSE
AccessioniPrimary (citable) accession number: P60469
Secondary accession number(s): B8QI35
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: October 14, 2015
Last modified: June 8, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.