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Protein

50S ribosomal protein L2

Gene

rplB

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L2UniRule annotation
Gene namesi
Name:rplBUniRule annotation
Ordered Locus Names:SAOUHSC_02509
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000008816 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001296201 – 27750S ribosomal protein L2Add BLAST277

Proteomic databases

PRIDEiP60430.

Interactioni

Subunit structurei

Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome.UniRule annotation

Protein-protein interaction databases

STRINGi93061.SAOUHSC_02509.

Structurei

Secondary structure

1277
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 11Combined sources4
Beta strandi13 – 15Combined sources3
Helixi22 – 24Combined sources3
Turni32 – 34Combined sources3
Beta strandi45 – 47Combined sources3
Beta strandi76 – 78Combined sources3
Beta strandi87 – 89Combined sources3
Beta strandi93 – 96Combined sources4
Beta strandi101 – 103Combined sources3
Beta strandi107 – 109Combined sources3
Beta strandi119 – 121Combined sources3
Beta strandi125 – 128Combined sources4
Beta strandi131 – 135Combined sources5
Beta strandi141 – 144Combined sources4
Turni148 – 150Combined sources3
Beta strandi157 – 159Combined sources3
Beta strandi169 – 173Combined sources5
Beta strandi183 – 185Combined sources3
Beta strandi190 – 193Combined sources4
Helixi200 – 202Combined sources3
Helixi210 – 213Combined sources4
Beta strandi222 – 225Combined sources4
Turni227 – 229Combined sources3
Beta strandi236 – 238Combined sources3
Beta strandi243 – 245Combined sources3
Beta strandi251 – 253Combined sources3
Helixi265 – 267Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WCEX-ray3.53A1-277[»]
4WF9X-ray3.43A1-277[»]
4WFAX-ray3.39A1-277[»]
4WFBX-ray3.43A1-277[»]
5HKVX-ray3.66A1-277[»]
5HL7X-ray3.55A1-277[»]
5LI0electron microscopy3.80D2-276[»]
5ND8electron microscopy3.70D1-277[»]
5ND9electron microscopy3.70D1-277[»]
5NRGX-ray3.44A1-277[»]
5TCUelectron microscopy3.90L22-275[»]
ProteinModelPortaliP60430.
SMRiP60430.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the universal ribosomal protein uL2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CFD. Bacteria.
COG0090. LUCA.
HOGENOMiHOG000232982.
KOiK02886.
OMAiHGYIKGV.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
4.10.950.10. 1 hit.
HAMAPiMF_01320_B. Ribosomal_L2_B. 1 hit.
InterProiView protein in InterPro
IPR012340. NA-bd_OB-fold.
IPR022666. Rbsml_prot_L2_RNA-bd_dom.
IPR014722. Rib_L2_dom2.
IPR002171. Ribosomal_L2.
IPR005880. Ribosomal_L2_bac/org-type.
IPR022669. Ribosomal_L2_C.
IPR022671. Ribosomal_L2_CS.
IPR014726. Ribosomal_L2_dom3.
IPR008991. Translation_prot_SH3-like.
PANTHERiPTHR13691. PTHR13691. 1 hit.
PfamiView protein in Pfam
PF00181. Ribosomal_L2. 1 hit.
PF03947. Ribosomal_L2_C. 1 hit.
PIRSFiPIRSF002158. Ribosomal_L2. 1 hit.
SMARTiView protein in SMART
SM01383. Ribosomal_L2. 1 hit.
SM01382. Ribosomal_L2_C. 1 hit.
SUPFAMiSSF50104. SSF50104. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR01171. rplB_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00467. RIBOSOMAL_L2. 1 hit.

Sequencei

Sequence statusi: Complete.

P60430-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIKKYKPIT NGRRNMTSLD FAEITKTTPE KSLLKPLPKK AGRNNQGKLT
60 70 80 90 100
VRHHGGGHKR QYRVIDFKRN KDGINAKVDS IQYDPNRSAN IALVVYADGE
110 120 130 140 150
KRYIIAPKGL EVGQIVESGA EADIKVGNAL PLQNIPVGTV VHNIELKPGK
160 170 180 190 200
GGQIARSAGA SAQVLGKEGK YVLIRLRSGE VRMILSTCRA TIGQVGNLQH
210 220 230 240 250
ELVNVGKAGR SRWKGIRPTV RGSVMNPNDH PHGGGEGRAP IGRPSPMSPW
260 270
GKPTLGKKTR RGKKSSDKLI VRGRKKK
Length:277
Mass (Da):30,155
Last modified:February 16, 2004 - v1
Checksum:iF29296D3EE1E146E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF205220 Genomic DNA. Translation: AAK37412.2.
CP000253 Genomic DNA. Translation: ABD31527.1.
RefSeqiWP_000985472.1. NC_007795.1.
YP_500976.1. NC_007795.1.

Genome annotation databases

EnsemblBacteriaiABD31527; ABD31527; SAOUHSC_02509.
GeneIDi31214981.
3920885.
KEGGisao:SAOUHSC_02509.
PATRICifig|93061.5.peg.2264.

Similar proteinsi

Entry informationi

Entry nameiRL2_STAA8
AccessioniPrimary (citable) accession number: P60430
Secondary accession number(s): Q2FW09, Q99S24, Q9AJ03
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: February 16, 2004
Last modified: October 25, 2017
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families