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P60405 (RL2_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
50S ribosomal protein L2
Gene names
Name:rplB
Ordered Locus Names:TTHA1689
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length276 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial By similarity. Makes several contacts with the 16S rRNA (forming bridge B7b) in the 70S ribosome. HAMAP-Rule MF_01320_B

Subunit structure

Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome, contacting the 16S rRNA. May also make transient contacts with protein S6 during translation in the 70S ribosome. Ref.5

Sequence similarities

Belongs to the ribosomal protein L2P family.

Mass spectrometry

Molecular mass is 30337 Da from positions 2 - 276. Determined by MALDI. Ref.3

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.2
Chain2 – 27627550S ribosomal protein L2 HAMAP-Rule MF_01320_B
PRO_0000129641

Secondary structure

............................................................................. 276
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P60405 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 7CF40C2BD586349B

FASTA27630,468
        10         20         30         40         50         60 
MAVKKFKPYT PSRRFMTVAD FSEITKTEPE KSLVKPLKKT GGRNNQGRIT VRFRGGGHKR 

        70         80         90        100        110        120 
LYRIIDFKRW DKVGIPAKVA AIEYDPNRSA RIALLHYVDG EKRYIIAPDG LQVGQQVVAG 

       130        140        150        160        170        180 
PDAPIQVGNA LPLRFIPVGT VVHAVELEPK KGAKLARAAG TSAQIQGREG DYVILRLPSG 

       190        200        210        220        230        240 
ELRKVHGECY ATVGAVGNAD HKNIVLGKAG RSRWLGRRPH VRGAAMNPVD HPHGGGEGRA 

       250        260        270 
PRGRPPASPW GWQTKGLKTR KRRKPSSRFI IARRKK 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
[2]"Identification of the 50S ribosomal proteins from the eubacterium Thermus thermophilus."
Katsani K.R., Tsiboli P., Anagnostopoulos K., Urlaub H., Choli-Papadopoulou T.
Biol. Chem. 381:1079-1087(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-22.
[3]"Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry."
Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A.
Proteomics 5:4818-4831(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: MASS SPECTROMETRY.
[4]"The path of messenger RNA through the ribosome."
Yusupova G.Z., Yusupov M.M., Cate J.H.D., Noller H.F.
Cell 106:233-241(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (5.0 ANGSTROMS) OF THE RIBOSOME.
[5]"Crystal structure of the ribosome at 5.5 A resolution."
Yusupov M.M., Yusupova G.Z., Baucom A., Lieberman K., Earnest T.N., Cate J.H.D., Noller H.F.
Science 292:883-896(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF THE RIBOSOME, INTERSUBUNIT BRIDGE FORMATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008226 Genomic DNA. Translation: BAD71512.1.
RefSeqYP_144955.1. NC_006461.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GIYX-ray5.50D62-234[»]
1YL3X-ray5.50D62-234[»]
2HGJX-ray5.00D1-276[»]
2HGQX-ray5.50D1-276[»]
2HGUX-ray4.51D1-276[»]
2J01X-ray2.80D2-276[»]
2J03X-ray2.80D2-276[»]
2V47X-ray3.50D2-276[»]
2V49X-ray3.50D2-276[»]
2WDIX-ray3.30D1-276[»]
2WDJX-ray3.30D1-276[»]
2WDLX-ray3.50D1-276[»]
2WDNX-ray3.50D1-276[»]
2WH2X-ray3.45D1-276[»]
2WH4X-ray3.45D1-276[»]
2WRJX-ray3.60D1-276[»]
2WRLX-ray3.60D1-276[»]
2WROX-ray3.60D1-276[»]
2WRRX-ray3.60D1-276[»]
2X9SX-ray3.10D1-276[»]
2X9UX-ray3.10D1-276[»]
2XG0X-ray3.20D1-276[»]
2XG2X-ray3.20D1-276[»]
2XQEX-ray3.10D1-276[»]
2XTGelectron microscopy7.80D1-276[»]
2XUXelectron microscopy7.60D1-276[»]
2Y0VX-ray3.10D1-276[»]
2Y0XX-ray3.10D1-276[»]
2Y0ZX-ray3.10D1-276[»]
2Y11X-ray3.10D1-276[»]
2Y13X-ray3.10D1-276[»]
2Y15X-ray3.10D1-276[»]
2Y17X-ray3.10D1-276[»]
2Y19X-ray3.10D1-276[»]
3FINelectron microscopy6.40D2-273[»]
3HUXX-ray3.10D1-276[»]
3HUZX-ray3.10D1-276[»]
3I8FX-ray3.10D1-276[»]
3I8IX-ray3.10D1-276[»]
3I9CX-ray3.50D1-276[»]
3I9EX-ray3.50D1-276[»]
3KIRX-ray3.30D1-276[»]
3KITX-ray3.30D1-276[»]
3KIWX-ray3.60D1-276[»]
3KIYX-ray3.60D1-276[»]
3KNIX-ray3.00D1-276[»]
3KNKX-ray3.00D1-276[»]
3KNMX-ray3.45D1-276[»]
3KNOX-ray3.45D1-276[»]
3OH5X-ray3.00D1-276[»]
3OH7X-ray3.00D1-276[»]
3OHJX-ray3.00D1-276[»]
3OHKX-ray3.00D1-276[»]
3OHZX-ray3.00D1-276[»]
3OI1X-ray3.00D1-276[»]
3OI3X-ray3.10D1-276[»]
3OI5X-ray3.10D1-276[»]
3TVEX-ray3.10D2-273[»]
3TVHX-ray3.10D2-273[»]
3UXQX-ray3.20D1-276[»]
3UXRX-ray3.20D1-276[»]
3UYEX-ray3.00D1-276[»]
3UYGX-ray3.00D1-276[»]
3UZ1X-ray3.30D1-276[»]
3UZ2X-ray3.30D1-276[»]
3UZ8X-ray3.00D1-276[»]
3UZ9X-ray3.00D1-276[»]
3UZFX-ray3.30D1-276[»]
3UZHX-ray3.30D1-276[»]
3UZKX-ray3.30D1-276[»]
3UZNX-ray3.30D1-276[»]
3V23X-ray3.00D1-276[»]
3V25X-ray3.00D1-276[»]
3V27X-ray3.10D1-276[»]
3V29X-ray3.10D1-276[»]
3V2DX-ray2.70D1-276[»]
3V2FX-ray2.70D1-276[»]
3V6WX-ray3.90D1-276[»]
3V6XX-ray3.90D1-276[»]
3ZN9X-ray3.10D1-276[»]
3ZNEX-ray3.10D1-276[»]
3ZVPX-ray3.80D1-276[»]
4ABSX-ray3.10D1-276[»]
4B8GX-ray3.70D1-276[»]
4B8IX-ray3.70D1-276[»]
4BTDX-ray2.95D2-276[»]
4BYCX-ray3.35D1-276[»]
4BYEX-ray3.35D1-276[»]
4DHAX-ray3.20D1-276[»]
4DHCX-ray3.20D1-276[»]
4EJBX-ray3.52D1-276[»]
4EJCX-ray3.52D1-276[»]
4G5LX-ray3.30D1-276[»]
4G5NX-ray3.30D1-276[»]
4G5UX-ray3.10D1-276[»]
4G5WX-ray3.10D1-276[»]
4JUXX-ray2.86D1-276[»]
4K0MX-ray3.30D2-272[»]
4K0QX-ray3.30D2-272[»]
4NVVX-ray3.00D1-276[»]
4NVXX-ray3.00D1-276[»]
4NVZX-ray3.10D1-276[»]
4NW1X-ray3.10D1-276[»]
ProteinModelPortalP60405.
SMRP60405. Positions 2-273.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING300852.TTHA1689.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD71512; BAD71512; BAD71512.
GeneID3168720.
KEGGttj:TTHA1689.
PATRIC23958333. VBITheThe93045_1659.

Phylogenomic databases

eggNOGCOG0090.
HOGENOMHOG000232982.
KOK02886.
OMASAMNPID.
OrthoDBEOG6TR0J1.
PhylomeDBP60405.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-1728-MONOMER.

Family and domain databases

Gene3D2.30.30.30. 1 hit.
2.40.50.140. 1 hit.
4.10.950.10. 1 hit.
HAMAPMF_01320_B. Ribosomal_L2_B.
InterProIPR012340. NA-bd_OB-fold.
IPR022666. Rbsml_prot_L2_RNA-bd_dom.
IPR014722. Rib_L2_dom2.
IPR002171. Ribosomal_L2.
IPR005880. Ribosomal_L2_bac/org-type.
IPR022669. Ribosomal_L2_C.
IPR022671. Ribosomal_L2_CS.
IPR014726. Ribosomal_L2_dom3.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PANTHERPTHR13691. PTHR13691. 1 hit.
PTHR13691:SF5. PTHR13691:SF5. 1 hit.
PfamPF00181. Ribosomal_L2. 1 hit.
PF03947. Ribosomal_L2_C. 1 hit.
[Graphical view]
PIRSFPIRSF002158. Ribosomal_L2. 1 hit.
SUPFAMSSF50104. SSF50104. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR01171. rplB_bact. 1 hit.
PROSITEPS00467. RIBOSOMAL_L2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP60405.

Entry information

Entry nameRL2_THET8
AccessionPrimary (citable) accession number: P60405
Secondary accession number(s): Q5SHP1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 97 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references