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P60405

- RL2_THET8

UniProt

P60405 - RL2_THET8

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Protein
50S ribosomal protein L2
Gene
rplB, TTHA1689
Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial By similarity. Makes several contacts with the 16S rRNA (forming bridge B7b) in the 70S ribosome.UniRule annotation

GO - Molecular functioni

  1. rRNA binding Source: UniProtKB-HAMAP
  2. structural constituent of ribosome Source: InterPro
  3. transferase activity Source: InterPro

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1728-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L2
Gene namesi
Name:rplB
Ordered Locus Names:TTHA1689
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. large ribosomal subunit Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 27627550S ribosomal protein L2UniRule annotation
PRO_0000129641Add
BLAST

Interactioni

Subunit structurei

Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome, contacting the 16S rRNA. May also make transient contacts with protein S6 during translation in the 70S ribosome.1 Publication

Protein-protein interaction databases

STRINGi300852.TTHA1689.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 53
Beta strandi8 – 103
Turni11 – 155
Beta strandi17 – 193
Turni22 – 243
Beta strandi26 – 283
Helixi31 – 333
Beta strandi34 – 363
Beta strandi43 – 486
Beta strandi49 – 546
Beta strandi61 – 633
Helixi70 – 723
Beta strandi77 – 837
Beta strandi88 – 903
Beta strandi92 – 976
Beta strandi102 – 1065
Beta strandi108 – 1103
Beta strandi119 – 1235
Beta strandi125 – 1284
Beta strandi130 – 1323
Helixi133 – 1353
Beta strandi141 – 1488
Turni149 – 1513
Beta strandi153 – 1564
Beta strandi158 – 1603
Beta strandi163 – 1697
Beta strandi172 – 1765
Beta strandi178 – 1803
Beta strandi182 – 1865
Beta strandi190 – 1945
Helixi199 – 2035
Helixi209 – 2146
Helixi223 – 2253
Turni228 – 2303
Beta strandi232 – 2343
Beta strandi236 – 2394
Beta strandi243 – 2453
Beta strandi249 – 2513
Beta strandi253 – 2564
Helixi265 – 2695
Beta strandi270 – 2723

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GIYX-ray5.50D62-234[»]
1YL3X-ray5.50D62-234[»]
2HGJX-ray5.00D1-276[»]
2HGQX-ray5.50D1-276[»]
2HGUX-ray4.51D1-276[»]
2J01X-ray2.80D2-276[»]
2J03X-ray2.80D2-276[»]
2V47X-ray3.50D2-276[»]
2V49X-ray3.50D2-276[»]
2WDIX-ray3.30D1-276[»]
2WDJX-ray3.30D1-276[»]
2WDLX-ray3.50D1-276[»]
2WDNX-ray3.50D1-276[»]
2WH2X-ray3.45D1-276[»]
2WH4X-ray3.45D1-276[»]
2WRJX-ray3.60D1-276[»]
2WRLX-ray3.60D1-276[»]
2WROX-ray3.60D1-276[»]
2WRRX-ray3.60D1-276[»]
2X9SX-ray3.10D1-276[»]
2X9UX-ray3.10D1-276[»]
2XG0X-ray3.20D1-276[»]
2XG2X-ray3.20D1-276[»]
2XQEX-ray3.10D1-276[»]
2XTGelectron microscopy7.80D1-276[»]
2XUXelectron microscopy7.60D1-276[»]
2Y0VX-ray3.10D1-276[»]
2Y0XX-ray3.10D1-276[»]
2Y0ZX-ray3.10D1-276[»]
2Y11X-ray3.10D1-276[»]
2Y13X-ray3.10D1-276[»]
2Y15X-ray3.10D1-276[»]
2Y17X-ray3.10D1-276[»]
2Y19X-ray3.10D1-276[»]
3FINelectron microscopy6.40D2-273[»]
3HUXX-ray3.10D1-276[»]
3HUZX-ray3.10D1-276[»]
3I8FX-ray3.10D1-276[»]
3I8IX-ray3.10D1-276[»]
3I9CX-ray3.50D1-276[»]
3I9EX-ray3.50D1-276[»]
3KIRX-ray3.30D1-276[»]
3KITX-ray3.30D1-276[»]
3KIWX-ray3.60D1-276[»]
3KIYX-ray3.60D1-276[»]
3KNIX-ray3.00D1-276[»]
3KNKX-ray3.00D1-276[»]
3KNMX-ray3.45D1-276[»]
3KNOX-ray3.45D1-276[»]
3OH5X-ray3.00D1-276[»]
3OH7X-ray3.00D1-276[»]
3OHJX-ray3.00D1-276[»]
3OHKX-ray3.00D1-276[»]
3OHZX-ray3.00D1-276[»]
3OI1X-ray3.00D1-276[»]
3OI3X-ray3.10D1-276[»]
3OI5X-ray3.10D1-276[»]
3TVEX-ray3.10D2-273[»]
3TVHX-ray3.10D2-273[»]
3UXQX-ray3.20D1-276[»]
3UXRX-ray3.20D1-276[»]
3UYEX-ray3.00D1-276[»]
3UYGX-ray3.00D1-276[»]
3UZ1X-ray3.30D1-276[»]
3UZ2X-ray3.30D1-276[»]
3UZ8X-ray3.00D1-276[»]
3UZ9X-ray3.00D1-276[»]
3UZFX-ray3.30D1-276[»]
3UZHX-ray3.30D1-276[»]
3UZKX-ray3.30D1-276[»]
3UZNX-ray3.30D1-276[»]
3V23X-ray3.00D1-276[»]
3V25X-ray3.00D1-276[»]
3V27X-ray3.10D1-276[»]
3V29X-ray3.10D1-276[»]
3V2DX-ray2.70D1-276[»]
3V2FX-ray2.70D1-276[»]
3V6WX-ray3.90D1-276[»]
3V6XX-ray3.90D1-276[»]
3ZN9X-ray3.10D1-276[»]
3ZNEX-ray3.10D1-276[»]
3ZVPX-ray3.80D1-276[»]
4ABSX-ray3.10D1-276[»]
4B8GX-ray3.70D1-276[»]
4B8IX-ray3.70D1-276[»]
4BTDX-ray2.95D2-276[»]
4BYCX-ray3.35D1-276[»]
4BYEX-ray3.35D1-276[»]
4DHAX-ray3.20D1-276[»]
4DHCX-ray3.20D1-276[»]
4EJBX-ray3.52D1-276[»]
4EJCX-ray3.52D1-276[»]
4G5LX-ray3.30D1-276[»]
4G5NX-ray3.30D1-276[»]
4G5UX-ray3.10D1-276[»]
4G5WX-ray3.10D1-276[»]
4JUXX-ray2.86D1-276[»]
4K0MX-ray3.30D2-272[»]
4K0QX-ray3.30D2-272[»]
4NVVX-ray3.00D1-276[»]
4NVXX-ray3.00D1-276[»]
4NVZX-ray3.10D1-276[»]
4NW1X-ray3.10D1-276[»]
ProteinModelPortaliP60405.
SMRiP60405. Positions 2-273.

Miscellaneous databases

EvolutionaryTraceiP60405.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0090.
HOGENOMiHOG000232982.
KOiK02886.
OMAiSAMNPID.
OrthoDBiEOG6TR0J1.
PhylomeDBiP60405.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
2.40.50.140. 1 hit.
4.10.950.10. 1 hit.
HAMAPiMF_01320_B. Ribosomal_L2_B.
InterProiIPR012340. NA-bd_OB-fold.
IPR022666. Rbsml_prot_L2_RNA-bd_dom.
IPR014722. Rib_L2_dom2.
IPR002171. Ribosomal_L2.
IPR005880. Ribosomal_L2_bac/org-type.
IPR022669. Ribosomal_L2_C.
IPR022671. Ribosomal_L2_CS.
IPR014726. Ribosomal_L2_dom3.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PANTHERiPTHR13691. PTHR13691. 1 hit.
PTHR13691:SF5. PTHR13691:SF5. 1 hit.
PfamiPF00181. Ribosomal_L2. 1 hit.
PF03947. Ribosomal_L2_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002158. Ribosomal_L2. 1 hit.
SUPFAMiSSF50104. SSF50104. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR01171. rplB_bact. 1 hit.
PROSITEiPS00467. RIBOSOMAL_L2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60405-1 [UniParc]FASTAAdd to Basket

« Hide

MAVKKFKPYT PSRRFMTVAD FSEITKTEPE KSLVKPLKKT GGRNNQGRIT    50
VRFRGGGHKR LYRIIDFKRW DKVGIPAKVA AIEYDPNRSA RIALLHYVDG 100
EKRYIIAPDG LQVGQQVVAG PDAPIQVGNA LPLRFIPVGT VVHAVELEPK 150
KGAKLARAAG TSAQIQGREG DYVILRLPSG ELRKVHGECY ATVGAVGNAD 200
HKNIVLGKAG RSRWLGRRPH VRGAAMNPVD HPHGGGEGRA PRGRPPASPW 250
GWQTKGLKTR KRRKPSSRFI IARRKK 276
Length:276
Mass (Da):30,468
Last modified:January 23, 2007 - v3
Checksum:i7CF40C2BD586349B
GO

Mass spectrometryi

Molecular mass is 30337 Da from positions 2 - 276. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008226 Genomic DNA. Translation: BAD71512.1.
RefSeqiWP_011173712.1. NC_006461.1.
YP_144955.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71512; BAD71512; BAD71512.
GeneIDi3168720.
KEGGittj:TTHA1689.
PATRICi23958333. VBITheThe93045_1659.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008226 Genomic DNA. Translation: BAD71512.1 .
RefSeqi WP_011173712.1. NC_006461.1.
YP_144955.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1GIY X-ray 5.50 D 62-234 [» ]
1YL3 X-ray 5.50 D 62-234 [» ]
2HGJ X-ray 5.00 D 1-276 [» ]
2HGQ X-ray 5.50 D 1-276 [» ]
2HGU X-ray 4.51 D 1-276 [» ]
2J01 X-ray 2.80 D 2-276 [» ]
2J03 X-ray 2.80 D 2-276 [» ]
2V47 X-ray 3.50 D 2-276 [» ]
2V49 X-ray 3.50 D 2-276 [» ]
2WDI X-ray 3.30 D 1-276 [» ]
2WDJ X-ray 3.30 D 1-276 [» ]
2WDL X-ray 3.50 D 1-276 [» ]
2WDN X-ray 3.50 D 1-276 [» ]
2WH2 X-ray 3.45 D 1-276 [» ]
2WH4 X-ray 3.45 D 1-276 [» ]
2WRJ X-ray 3.60 D 1-276 [» ]
2WRL X-ray 3.60 D 1-276 [» ]
2WRO X-ray 3.60 D 1-276 [» ]
2WRR X-ray 3.60 D 1-276 [» ]
2X9S X-ray 3.10 D 1-276 [» ]
2X9U X-ray 3.10 D 1-276 [» ]
2XG0 X-ray 3.20 D 1-276 [» ]
2XG2 X-ray 3.20 D 1-276 [» ]
2XQE X-ray 3.10 D 1-276 [» ]
2XTG electron microscopy 7.80 D 1-276 [» ]
2XUX electron microscopy 7.60 D 1-276 [» ]
2Y0V X-ray 3.10 D 1-276 [» ]
2Y0X X-ray 3.10 D 1-276 [» ]
2Y0Z X-ray 3.10 D 1-276 [» ]
2Y11 X-ray 3.10 D 1-276 [» ]
2Y13 X-ray 3.10 D 1-276 [» ]
2Y15 X-ray 3.10 D 1-276 [» ]
2Y17 X-ray 3.10 D 1-276 [» ]
2Y19 X-ray 3.10 D 1-276 [» ]
3FIN electron microscopy 6.40 D 2-273 [» ]
3HUX X-ray 3.10 D 1-276 [» ]
3HUZ X-ray 3.10 D 1-276 [» ]
3I8F X-ray 3.10 D 1-276 [» ]
3I8I X-ray 3.10 D 1-276 [» ]
3I9C X-ray 3.50 D 1-276 [» ]
3I9E X-ray 3.50 D 1-276 [» ]
3KIR X-ray 3.30 D 1-276 [» ]
3KIT X-ray 3.30 D 1-276 [» ]
3KIW X-ray 3.60 D 1-276 [» ]
3KIY X-ray 3.60 D 1-276 [» ]
3KNI X-ray 3.00 D 1-276 [» ]
3KNK X-ray 3.00 D 1-276 [» ]
3KNM X-ray 3.45 D 1-276 [» ]
3KNO X-ray 3.45 D 1-276 [» ]
3OH5 X-ray 3.00 D 1-276 [» ]
3OH7 X-ray 3.00 D 1-276 [» ]
3OHJ X-ray 3.00 D 1-276 [» ]
3OHK X-ray 3.00 D 1-276 [» ]
3OHZ X-ray 3.00 D 1-276 [» ]
3OI1 X-ray 3.00 D 1-276 [» ]
3OI3 X-ray 3.10 D 1-276 [» ]
3OI5 X-ray 3.10 D 1-276 [» ]
3TVE X-ray 3.10 D 2-273 [» ]
3TVH X-ray 3.10 D 2-273 [» ]
3UXQ X-ray 3.20 D 1-276 [» ]
3UXR X-ray 3.20 D 1-276 [» ]
3UYE X-ray 3.00 D 1-276 [» ]
3UYG X-ray 3.00 D 1-276 [» ]
3UZ1 X-ray 3.30 D 1-276 [» ]
3UZ2 X-ray 3.30 D 1-276 [» ]
3UZ8 X-ray 3.00 D 1-276 [» ]
3UZ9 X-ray 3.00 D 1-276 [» ]
3UZF X-ray 3.30 D 1-276 [» ]
3UZH X-ray 3.30 D 1-276 [» ]
3UZK X-ray 3.30 D 1-276 [» ]
3UZN X-ray 3.30 D 1-276 [» ]
3V23 X-ray 3.00 D 1-276 [» ]
3V25 X-ray 3.00 D 1-276 [» ]
3V27 X-ray 3.10 D 1-276 [» ]
3V29 X-ray 3.10 D 1-276 [» ]
3V2D X-ray 2.70 D 1-276 [» ]
3V2F X-ray 2.70 D 1-276 [» ]
3V6W X-ray 3.90 D 1-276 [» ]
3V6X X-ray 3.90 D 1-276 [» ]
3ZN9 X-ray 3.10 D 1-276 [» ]
3ZNE X-ray 3.10 D 1-276 [» ]
3ZVP X-ray 3.80 D 1-276 [» ]
4ABS X-ray 3.10 D 1-276 [» ]
4B8G X-ray 3.70 D 1-276 [» ]
4B8I X-ray 3.70 D 1-276 [» ]
4BTD X-ray 2.95 D 2-276 [» ]
4BYC X-ray 3.35 D 1-276 [» ]
4BYE X-ray 3.35 D 1-276 [» ]
4DHA X-ray 3.20 D 1-276 [» ]
4DHC X-ray 3.20 D 1-276 [» ]
4EJB X-ray 3.52 D 1-276 [» ]
4EJC X-ray 3.52 D 1-276 [» ]
4G5L X-ray 3.30 D 1-276 [» ]
4G5N X-ray 3.30 D 1-276 [» ]
4G5U X-ray 3.10 D 1-276 [» ]
4G5W X-ray 3.10 D 1-276 [» ]
4JUX X-ray 2.86 D 1-276 [» ]
4K0M X-ray 3.30 D 2-272 [» ]
4K0Q X-ray 3.30 D 2-272 [» ]
4NVV X-ray 3.00 D 1-276 [» ]
4NVX X-ray 3.00 D 1-276 [» ]
4NVZ X-ray 3.10 D 1-276 [» ]
4NW1 X-ray 3.10 D 1-276 [» ]
ProteinModelPortali P60405.
SMRi P60405. Positions 2-273.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 300852.TTHA1689.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD71512 ; BAD71512 ; BAD71512 .
GeneIDi 3168720.
KEGGi ttj:TTHA1689.
PATRICi 23958333. VBITheThe93045_1659.

Phylogenomic databases

eggNOGi COG0090.
HOGENOMi HOG000232982.
KOi K02886.
OMAi SAMNPID.
OrthoDBi EOG6TR0J1.
PhylomeDBi P60405.

Enzyme and pathway databases

BioCyci TTHE300852:GH8R-1728-MONOMER.

Miscellaneous databases

EvolutionaryTracei P60405.

Family and domain databases

Gene3Di 2.30.30.30. 1 hit.
2.40.50.140. 1 hit.
4.10.950.10. 1 hit.
HAMAPi MF_01320_B. Ribosomal_L2_B.
InterProi IPR012340. NA-bd_OB-fold.
IPR022666. Rbsml_prot_L2_RNA-bd_dom.
IPR014722. Rib_L2_dom2.
IPR002171. Ribosomal_L2.
IPR005880. Ribosomal_L2_bac/org-type.
IPR022669. Ribosomal_L2_C.
IPR022671. Ribosomal_L2_CS.
IPR014726. Ribosomal_L2_dom3.
IPR008991. Translation_prot_SH3-like.
[Graphical view ]
PANTHERi PTHR13691. PTHR13691. 1 hit.
PTHR13691:SF5. PTHR13691:SF5. 1 hit.
Pfami PF00181. Ribosomal_L2. 1 hit.
PF03947. Ribosomal_L2_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF002158. Ribosomal_L2. 1 hit.
SUPFAMi SSF50104. SSF50104. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsi TIGR01171. rplB_bact. 1 hit.
PROSITEi PS00467. RIBOSOMAL_L2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.
  2. "Identification of the 50S ribosomal proteins from the eubacterium Thermus thermophilus."
    Katsani K.R., Tsiboli P., Anagnostopoulos K., Urlaub H., Choli-Papadopoulou T.
    Biol. Chem. 381:1079-1087(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-22.
  3. "Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry."
    Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A.
    Proteomics 5:4818-4831(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: MASS SPECTROMETRY.
  4. "The path of messenger RNA through the ribosome."
    Yusupova G.Z., Yusupov M.M., Cate J.H.D., Noller H.F.
    Cell 106:233-241(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (5.0 ANGSTROMS) OF THE RIBOSOME.
  5. Cited for: X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF THE RIBOSOME, INTERSUBUNIT BRIDGE FORMATION.

Entry informationi

Entry nameiRL2_THET8
AccessioniPrimary (citable) accession number: P60405
Secondary accession number(s): Q5SHP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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