P60400 (RL2_CORDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
50S ribosomal protein L2

Gene names

Name:	rplB
Ordered Locus Names:	DIP0476

Organism

Corynebacterium diphtheriae [Complete proteome] [HAMAP]

Taxonomic identifier

1717 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome By similarity. HAMAP MF_01320_B
Subunit structure	Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome By similarity.
Sequence similarities	Belongs to the ribosomal protein L2P family.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: InterPro
Cellular component	large ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular function	rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 280	280	50S ribosomal protein L2 HAMAP MF_01320_B		PRO_0000129554

Sequences

	Sequence		Length	Mass (Da)
	P60400 [UniParc]. Last modified February 16, 2004. Version 1. Checksum: 2DCD5E6669AD27A3	FASTA	280	31,094
	10 20 30 40 50 60 MAIRKYKPTT PGRRQSSVSM FEEITRSTPE KSLVRPLPKK GGRNVHGHIT VRHKGGGHKR 70 80 90 100 110 120 QYRLIDFRRN DKDGIPAKVA HIEYDPNRTA NIALLHYVDG EKRYIIAPKG LQQGQMIESG 130 140 150 160 170 180 VNADIKVGNN LPLRNIPAGT TIHCVELKPG AGAKLARSAG ASIQLLGKAG KYAVLRMPSS 190 200 210 220 230 240 EIRRVDIRCR ATVGEVGNAD QINIRWGKAG RMRWKGVRPT VRGVVMNPVD HPHGGGEGKT 250 260 270 280 SGGRHPVSPW GKKEGRTRNP NRYSNNMIVQ RRRTNKSKKR

References

[1]

"The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129." Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G., Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D., De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K., Moule S. , Quail M.A., Rabbinowitsch E., Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G., Parkhill J. Nucleic Acids Res. 31:6516-6523(2003) [PubMed: 14602910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248355 Genomic DNA. Translation: CAE48981.1.
RefSeq	NP_938855.1. NC_002935.2.
3D structure databases
ProteinModelPortal	P60400.
SMR	P60400. Positions 2-271.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2649035.
GenomeReviews	Gene locus DIP0476 in contig BX248353_GR.
KEGG	cdi:DIP0476.
NMPDR	fig|257309.1.peg.446.
PATRIC	21482121. VBICorDip47633_0461.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG748739.
OMA	QSNINWG.
PhylomeDB	P60400.
ProtClustDB	PRK09374.
Enzyme and pathway databases
BioCyc	CDIP257309:DIP0476-MONOMER.
Family and domain databases
HAMAP	MF_01320_B. Ribosomal_L2_B. [Tree]
InterPro	IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR022666. Rbsml_prot_L2_RNA-bd_dom. IPR002171. Ribosomal_L2. IPR014726. Ribosomal_L2_3. IPR005880. Ribosomal_L2_bac-type. IPR022669. Ribosomal_L2_C. IPR022671. Ribosomal_L2_CS. IPR014722. Transl_SH3-like_sub. IPR008991. Translation_prot_SH3-like. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:2.30.30.30. Ribosomal_L2. 1 hit. G3DSA:4.10.950.10. Ribosomal_L2. 1 hit.
KO	K02886.
PANTHER	PTHR13691:SF5. Ribosom_L2_bac. 1 hit. PTHR13691. Ribosomal_L2. 1 hit.
Pfam	PF00181. Ribosomal_L2. 1 hit. PF03947. Ribosomal_L2_C. 1 hit. [Graphical view]
PIRSF	PIRSF002158. Ribosomal_L2. 1 hit.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF50104. Transl_SH3_like. 1 hit.
TIGRFAMs	TIGR01171. RplB_bact. 1 hit.
PROSITE	PS00467. RIBOSOMAL_L2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RL2_CORDI

Accession

Primary (citable) accession number: P60400

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	February 16, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program