P60395 (RSMH_CORDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	DIP1606

Organism

Corynebacterium diphtheriae [Complete proteome] [HAMAP]

Taxonomic identifier

1717 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000108613
Regions
	Region	52 – 54	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	71	1	S-adenosyl-L-methionine By similarity
	Binding site	98	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	130	1	S-adenosyl-L-methionine By similarity
	Binding site	137	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P60395 [UniParc]. Last modified February 16, 2004. Version 1. Checksum: F2A6F15DDD26D1B0	FASTA	339	37,408
	10 20 30 40 50 60 MTHGEPQEFS FDVEENHGHV PVLRARMAEL IAPKVTAMGS DAVIIDGTLG AGGHSKYFLE 70 80 90 100 110 120 SFPEARVIGL DRDTNSLASA RERLKEFGDR FLGIHTRFDR FAPKLEELAQ SGNPIAATAL 130 140 150 160 170 180 EQGISGALFD LGVSSMQLDQ AERGFAYRVD APLDMRMDNT IGITAADVLN TYSHGELARI 190 200 210 220 230 240 LKTYGDERFA GKIASAIVRE REKEPFDTSA RLVELLYATI PAATRRTGGH PAKRTFQALR 250 260 270 280 290 300 IEVNRELESL ENVLPEITQR LTTGGRAVFM SYQSLEDKIV KRYFVDISTS KTPPGLPMEL 310 320 330 PEYAPKFKVV TRGAEKATDA EIEENPRAAS VRVRAIEAL

References

[1]

"The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129." Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G., Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D., De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K., Moule S. , Quail M.A., Rabbinowitsch E., Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G., Parkhill J. Nucleic Acids Res. 31:6516-6523(2003) [PubMed: 14602910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248358 Genomic DNA. Translation: CAE50131.1.
RefSeq	NP_939948.1. NC_002935.2.
3D structure databases
ProteinModelPortal	P60395.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2650795.
GenomeReviews	Gene locus DIP1606 in contig BX248353_GR.
KEGG	cdi:DIP1606.
NMPDR	fig|257309.1.peg.1539.
PATRIC	21484420. VBICorDip47633_1586.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG302779.
OMA	NPATRAF.
PhylomeDB	P60395.
ProtClustDB	PRK00050.
Enzyme and pathway databases
BioCyc	CDIP257309:DIP1606-MONOMER.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_CORDI

Accession

Primary (citable) accession number: P60395

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	February 16, 2004
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program