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Protein

Ribosomal RNA small subunit methyltransferase H

Gene

rsmH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA. In vitro, active on the assembled 30S subunit, but not naked 16S rRNA or 70S ribosomes.2 Publications

Catalytic activityi

S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei55 – 551S-adenosyl-L-methionineBy similarity
Binding sitei79 – 791S-adenosyl-L-methionine; via amide nitrogenBy similarity
Binding sitei101 – 1011S-adenosyl-L-methionineBy similarity
Binding sitei108 – 1081S-adenosyl-L-methionineBy similarity

GO - Molecular functioni

  • rRNA (cytosine-N4-)-methyltransferase activity Source: EcoCyc

GO - Biological processi

  • rRNA base methylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG11085-MONOMER.
ECOL316407:JW0080-MONOMER.
MetaCyc:EG11085-MONOMER.
BRENDAi2.1.1.199. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase H (EC:2.1.1.199)
Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH
Gene namesi
Name:rsmH
Synonyms:mraW, yabC
Ordered Locus Names:b0082, JW0080
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11085. rsmH.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoCyc
  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 313313Ribosomal RNA small subunit methyltransferase HPRO_0000108620Add
BLAST

Proteomic databases

EPDiP60390.
PaxDbiP60390.
PRIDEiP60390.

Interactioni

Protein-protein interaction databases

BioGridi4261639. 12 interactions.
DIPiDIP-35840N.
IntActiP60390. 6 interactions.
STRINGi511145.b0082.

Structurei

Secondary structure

1
313
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni10 – 123Combined sources
Helixi13 – 186Combined sources
Beta strandi26 – 305Combined sources
Helixi36 – 427Combined sources
Beta strandi50 – 567Combined sources
Helixi58 – 647Combined sources
Beta strandi72 – 776Combined sources
Helixi79 – 813Combined sources
Helixi82 – 887Combined sources
Beta strandi95 – 1017Combined sources
Helixi106 – 1105Combined sources
Helixi112 – 1143Combined sources
Beta strandi118 – 1203Combined sources
Helixi136 – 1427Combined sources
Helixi145 – 15612Combined sources
Helixi161 – 17414Combined sources
Helixi180 – 19011Combined sources
Helixi202 – 21312Combined sources
Helixi215 – 22915Combined sources
Beta strandi230 – 24213Combined sources
Helixi243 – 25513Combined sources
Beta strandi281 – 2877Combined sources
Helixi291 – 2966Combined sources
Helixi298 – 3003Combined sources
Beta strandi304 – 3107Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TKAX-ray2.25A1-313[»]
ProteinModelPortaliP60390.
SMRiP60390. Positions 8-313.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni35 – 373S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CGJ. Bacteria.
COG0275. LUCA.
HOGENOMiHOG000049778.
InParanoidiP60390.
KOiK03438.
OMAiADGCYLD.
PhylomeDBiP60390.

Family and domain databases

Gene3Di1.10.150.170. 1 hit.
3.40.50.150. 2 hits.
HAMAPiMF_01007. 16SrRNA_methyltr_H. 1 hit.
InterProiIPR002903. RsmH.
IPR023397. SAM-dep_MeTrfase_MraW_recog.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11265. PTHR11265. 1 hit.
PfamiPF01795. Methyltransf_5. 1 hit.
[Graphical view]
PIRSFiPIRSF004486. MraW. 1 hit.
SUPFAMiSSF53335. SSF53335. 2 hits.
SSF81799. SSF81799. 1 hit.
TIGRFAMsiTIGR00006. TIGR00006. 1 hit.

Sequencei

Sequence statusi: Complete.

P60390-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMENYKHTTV LLDEAVNGLN IRPDGIYIDG TFGRGGHSRL ILSQLGEEGR
60 70 80 90 100
LLAIDRDPQA IAVAKTIDDP RFSIIHGPFS ALGEYVAERD LIGKIDGILL
110 120 130 140 150
DLGVSSPQLD DAERGFSFMR DGPLDMRMDP TRGQSAAEWL QTAEEADIAW
160 170 180 190 200
VLKTYGEERF AKRIARAIVE RNREQPMTRT KELAEVVAAA TPVKDKFKHP
210 220 230 240 250
ATRTFQAVRI WVNSELEEIE QALKSSLNVL APGGRLSIIS FHSLEDRIVK
260 270 280 290 300
RFMRENSRGP QVPAGLPMTE EQLKKLGGRQ LRALGKLMPG EEEVAENPRA
310
RSSVLRIAER TNA
Length:313
Mass (Da):34,878
Last modified:February 16, 2004 - v1
Checksum:i43174C907A36ECC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52063 Genomic DNA. Translation: CAA36283.1.
X55034 Genomic DNA. Translation: CAA38859.1.
U00096 Genomic DNA. Translation: AAC73193.1.
AP009048 Genomic DNA. Translation: BAB96650.1.
K00137 Genomic DNA. No translation available.
PIRiJH0092. QQECFT.
RefSeqiNP_414624.1. NC_000913.3.
WP_000970479.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73193; AAC73193; b0082.
BAB96650; BAB96650; BAB96650.
GeneIDi944806.
KEGGiecj:JW0080.
eco:b0082.
PATRICi32115269. VBIEscCol129921_0086.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52063 Genomic DNA. Translation: CAA36283.1.
X55034 Genomic DNA. Translation: CAA38859.1.
U00096 Genomic DNA. Translation: AAC73193.1.
AP009048 Genomic DNA. Translation: BAB96650.1.
K00137 Genomic DNA. No translation available.
PIRiJH0092. QQECFT.
RefSeqiNP_414624.1. NC_000913.3.
WP_000970479.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TKAX-ray2.25A1-313[»]
ProteinModelPortaliP60390.
SMRiP60390. Positions 8-313.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261639. 12 interactions.
DIPiDIP-35840N.
IntActiP60390. 6 interactions.
STRINGi511145.b0082.

Proteomic databases

EPDiP60390.
PaxDbiP60390.
PRIDEiP60390.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73193; AAC73193; b0082.
BAB96650; BAB96650; BAB96650.
GeneIDi944806.
KEGGiecj:JW0080.
eco:b0082.
PATRICi32115269. VBIEscCol129921_0086.

Organism-specific databases

EchoBASEiEB1077.
EcoGeneiEG11085. rsmH.

Phylogenomic databases

eggNOGiENOG4105CGJ. Bacteria.
COG0275. LUCA.
HOGENOMiHOG000049778.
InParanoidiP60390.
KOiK03438.
OMAiADGCYLD.
PhylomeDBiP60390.

Enzyme and pathway databases

BioCyciEcoCyc:EG11085-MONOMER.
ECOL316407:JW0080-MONOMER.
MetaCyc:EG11085-MONOMER.
BRENDAi2.1.1.199. 2026.

Miscellaneous databases

PROiP60390.

Family and domain databases

Gene3Di1.10.150.170. 1 hit.
3.40.50.150. 2 hits.
HAMAPiMF_01007. 16SrRNA_methyltr_H. 1 hit.
InterProiIPR002903. RsmH.
IPR023397. SAM-dep_MeTrfase_MraW_recog.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11265. PTHR11265. 1 hit.
PfamiPF01795. Methyltransf_5. 1 hit.
[Graphical view]
PIRSFiPIRSF004486. MraW. 1 hit.
SUPFAMiSSF53335. SSF53335. 2 hits.
SSF81799. SSF81799. 1 hit.
TIGRFAMsiTIGR00006. TIGR00006. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRSMH_ECOLI
AccessioniPrimary (citable) accession number: P60390
Secondary accession number(s): P18595
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: February 16, 2004
Last modified: September 7, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.