Ribosomal RNA small subunit methyltransferase H - Escherichia coli (strain K12)

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P60390 (RSMH_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 88. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW, yabC
Ordered Locus Names:	b0082, JW0080

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA. In vitro, active on the assembled 30S subunit, but not naked 16S rRNA or 70S ribosomes. Ref.6 Ref.8
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. Ref.8
Subcellular location	Cytoplasm Ref.6.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	rRNA base methylation Inferred from mutant phenotype Ref.8. Source: EcoCyc
Cellular_component	cytoplasm Inferred from direct assay Ref.6. Source: EcoCyc
Molecular_function	rRNA (cytosine-N4-)-methyltransferase activity Inferred from direct assay Ref.8. Source: EcoCyc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
prs	P0A717	1	EBI-526651,EBI-906827

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 313

313

Ribosomal RNA small subunit methyltransferase H HAMAP-Rule MF_01007

PRO_0000108620

Regions

Region

35 – 37

S-adenosyl-L-methionine binding By similarity

Sites

Binding site

S-adenosyl-L-methionine By similarity

Binding site

S-adenosyl-L-methionine; via amide nitrogen By similarity

Binding site

101

S-adenosyl-L-methionine By similarity

Binding site

108

S-adenosyl-L-methionine By similarity

Secondary structure

313

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P60390 [UniParc].

Last modified February 16, 2004. Version 1.
Checksum: 43174C907A36ECC1
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FASTA

313

34,878

        10         20         30         40         50         60 
MMENYKHTTV LLDEAVNGLN IRPDGIYIDG TFGRGGHSRL ILSQLGEEGR LLAIDRDPQA 

        70         80         90        100        110        120 
IAVAKTIDDP RFSIIHGPFS ALGEYVAERD LIGKIDGILL DLGVSSPQLD DAERGFSFMR 

       130        140        150        160        170        180 
DGPLDMRMDP TRGQSAAEWL QTAEEADIAW VLKTYGEERF AKRIARAIVE RNREQPMTRT 

       190        200        210        220        230        240 
KELAEVVAAA TPVKDKFKHP ATRTFQAVRI WVNSELEEIE QALKSSLNVL APGGRLSIIS 

       250        260        270        280        290        300 
FHSLEDRIVK RFMRENSRGP QVPAGLPMTE EQLKKLGGRQ LRALGKLMPG EEEVAENPRA 

       310 
RSSVLRIAER TNA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the regulatory region of the gene pbpB of Escherichia coli." Gomez M.J., Fluoret B., van Heijenoort J., Ayala J.A. Nucleic Acids Res. 18:2813-2813(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"On the process of cellular division in Escherichia coli: nucleotide sequence of the gene for penicillin-binding protein 3." Nakamura M., Maruyama I.N., Soma M., Kato J., Suzuki H., Horota Y. Mol. Gen. Genet. 191:1-9(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 232-313. Strain: K12.
[6]	"mraW, an essential gene at the dcw cluster of Escherichia coli codes for a cytoplasmic protein with methyltransferase activity." Carrion M., Gomez M.J., Merchante-Schubert R., Dongarra S., Ayala J.A. Biochimie 81:879-888(1999) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-16, FUNCTION, SUBCELLULAR LOCATION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography." Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B. Electrophoresis 20:2181-2195(1999) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY. Strain: B / BL21.
[8]	"Fine-tuning of the ribosomal decoding center by conserved methyl-modifications in the Escherichia coli 16S rRNA." Kimura S., Suzuki T. Nucleic Acids Res. 38:1341-1352(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY. Strain: K12.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X52063 Genomic DNA. Translation: CAA36283.1.
X55034 Genomic DNA. Translation: CAA38859.1.
U00096 Genomic DNA. Translation: AAC73193.1.
AP009048 Genomic DNA. Translation: BAB96650.1.
K00137 Genomic DNA. No translation available.

PIR

QQECFT. JH0092.

RefSeq

NP_414624.1. NC_000913.2.
YP_488387.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3TKA	X-ray	2.25	A	1-313	[»]

ProteinModelPortal

P60390.

SMR

P60390. Positions 8-313.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-35840N.

IntAct

P60390. 5 interactions.

STRING

511145.b0082.

Proteomic databases

PaxDb

P60390.

PRIDE

P60390.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC73193; AAC73193; b0082.
BAB96650; BAB96650; BAB96650.

GeneID

12932645.
944806.

KEGG

ecj:Y75_p0081.
eco:b0082.

PATRIC

32115269. VBIEscCol129921_0086.

Organism-specific databases

EchoBASE

EB1077.

EcoGene

EG11085. rsmH.

Phylogenomic databases

eggNOG

COG0275.

HOGENOM

HOG000049778.

K03438.

OMA

NPATRAF.

ProtClustDB

PRK00050.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11085-MONOMER.
ECOL316407:JW0080-MONOMER.
MetaCyc:EG11085-MONOMER.

Gene expression databases

Genevestigator

P60390.

Family and domain databases

Gene3D

1.10.150.170. 1 hit.

HAMAP

MF_01007. 16SrRNA_methyltr_H.

InterPro

IPR002903. SAM-dep_MeTrfase_MraW.
IPR023397. SAM-dep_MeTrfase_MraW_recog.
[Graphical view]

PANTHER

PTHR11265. PTHR11265. 1 hit.

Pfam

PF01795. Methyltransf_5. 1 hit.
[Graphical view]

PIRSF

PIRSF004486. MraW. 1 hit.

SUPFAM

SSF81799. SSF81799. 1 hit.

TIGRFAMs

TIGR00006. TIGR00006. 1 hit.

ProtoNet

Search...

Entry information

Entry name

RSMH_ECOLI

Accession

Primary (citable) accession number: P60390
Secondary accession number(s): P18595

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	February 16, 2004
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents