P60381 (SPX_STRP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Regulatory protein spx

Gene names

Name:	spxA
Ordered Locus Names:	SPy_1249, M5005_Spy0959

Organism

Streptococcus pyogenes serotype M1 [Complete proteome] [HAMAP]

Taxonomic identifier

301447 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	134 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Interferes with activator-stimulated transcription by interaction with the RNA polymerase alpha-CTD. May function to globally reduce transcription of genes involved in growth- and development-promoting processes and to increase transcription of genes involved in thiol homeostasis, during periods of extreme stress By similarity. HAMAP MF_01132
Subcellular location	Cytoplasm Probable HAMAP MF_01132.
Sequence similarities	Belongs to the ArsC family. Spx subfamily.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm
Domain	Redox-active center
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 134	134	Regulatory protein spx HAMAP MF_01132		PRO_0000162577
Amino acid modifications
	Disulfide bond	10 ↔ 13		Redox-active Potential

Sequences

	Sequence		Length	Mass (Da)
	P60381 [UniParc]. Last modified February 16, 2004. Version 1. Checksum: 41461D18FEACB14E	FASTA	134	15,528
	10 20 30 40 50 60 MVTLFLSPSC TSCRKARAWL VKHEVDFQEH NIITSPLSRD ELMSILSFTE NGTEDIISTR 70 80 90 100 110 120 SKVFQKLDID VEELSISDLI DLIAKNPSLL RRPIIMDQKR MQIGFNEDEI RAFLSRDYRK 130 QELRQATIKA EIEG

References

[1]

"Complete genome sequence of an M1 strain of Streptococcus pyogenes." Ferretti J.J., McShan W.M., Ajdic D.J., Savic D.J., Savic G., Lyon K., Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P., Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L. , White J., Yuan X., Clifton S.W., Roe B.A., McLaughlin R.E. Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001) [PubMed: 11296296] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700294 / SF370 / Serotype M1.

[2]

"Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events." Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M. J. Infect. Dis. 192:771-782(2005) [PubMed: 16088826] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004092 Genomic DNA. Translation: AAK34104.1. CP000017 Genomic DNA. Translation: AAZ51577.1.
RefSeq	NP_269383.1. NC_002737.1. YP_282322.1. NC_007297.1.
3D structure databases
ProteinModelPortal	P60381.
SMR	P60381. Positions 1-116.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000000985; EBSTRP00000000985; EBSTRG00000000985. EBSTRT00000028578; EBSTRP00000027596; EBSTRG00000028578.
GeneID	3571934. 901349.
GenomeReviews	Gene locus SPy_1249 in contig AE004092_GR. Gene locus M5005_Spy0959 in contig CP000017_GR.
KEGG	spy:SPy_1249. spz:M5005_Spy_0959.
PATRIC	19716055. VBIStrPyo79812_1092.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000027616.
HOGENOM	HBG734370.
OMA	FTERNIF.
ProtClustDB	PRK01655.
Enzyme and pathway databases
BioCyc	SPYO160490:SPY1249-MONOMER. SPYO293653:M5005_SPY0959-MONOMER.
Family and domain databases
HAMAP	MF_01132. SpxA. [Tree]
InterPro	IPR006660. Arsenate_reductase-like. IPR012336. Thioredoxin-like_fold. IPR023731. Tscrpt_reg_Spx. IPR006504. Tscrpt_reg_Spx/MgsR. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
KO	K00537.
Pfam	PF03960. ArsC. 1 hit. [Graphical view]
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
TIGRFAMs	TIGR01617. ArsC_related. 1 hit.
PROSITE	PS51353. ARSC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPX_STRP1

Accession

Primary (citable) accession number: P60381
Secondary accession number(s): Q48YJ5, Q99ZF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	February 16, 2004
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program