Reviewed, UniProtKB/Swiss-Prot P60378 (SPX_STAAM)

Names and origin

Protein names

Recommended name:
    Regulatory protein spx

Gene names

Name:	spxA
Ordered Locus Names:	SAV0997

Organism

Staphylococcus aureus (strain Mu50 / ATCC 700699) [Complete proteome] [HAMAP]

Taxonomic identifier

158878 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	131 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Interferes with activator-stimulated transcription by interaction with the RNA polymerase alpha-CTD. May function to globally reduce transcription of genes involved in growth- and development-promoting processes and to increase transcription of genes involved in thiol homeostasis, during periods of extreme stress By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the arsC family. Spx subfamily.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm
Domain	Redox-active center
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transcription regulator activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 131	131	Regulatory protein spx HAMAP MF_01132		PRO_0000162563
Amino acid modifications
	Disulfide bond	10 ↔ 13		Redox-active Potential

Sequences

	Sequence		Length	Mass (Da)
	P60378-1 [UniParc]. Last modified February 16, 2004. Version 1. Checksum: 89F67A54666699BF	FASTA	131	15,441
	10 20 30 40 50 60 MVTLFTSPSC TSCRKAKAWL QEHDIPYTER NIFSEHLTID EIKQILKMTE DGTDEIISTR 70 80 90 100 110 120 SKTYQKLNVD IDSLPLQDLY SIIQDNPGLL RRPIILDNKR LQVGYNEDEI RRFLPRKVRT 130 FQLQEAQRMV D

References

[1]

"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed: 11418146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000017 Genomic DNA. Translation: BAB57159.1.
RefSeq	NP_371521.1.
3D structure databases
SMR	P60378. Positions 1-118.
ModBase	Search...
2-D gel databases
World-2DPAGE	0002:P60378.
Genome annotation databases
GeneID	1120972.
GenomeReviews	Gene locus SAV0997 in contig BA000017_GR.
KEGG	sav:SAV0997.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P60378.
OMA	P60378. RRMQVGY.
Enzyme and pathway databases
BioCyc	SAUR158878:SAV0997-MON.
Family and domain databases
HAMAP	MF_01132. [Tree]
InterPro	IPR006660. Arsenate_reductase-like. IPR006504. CHP1617_ArsC_rel. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF03960. ArsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR01617. arsC_related. 1 hit.
PROSITE	PS51353. ARSC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPX_STAAM

Accession

Primary (citable) accession number: P60378
Secondary accession number(s): Q99V93

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	February 16, 2004
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)