P60377 (SPX_LISIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Regulatory protein spx

Gene names

Name:	spxA
Ordered Locus Names:	lin2295

Organism

Listeria innocua [Complete proteome] [HAMAP]

Taxonomic identifier

1642 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	131 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Interferes with activator-stimulated transcription by interaction with the RNA polymerase alpha-CTD. May function to globally reduce transcription of genes involved in growth- and development-promoting processes and to increase transcription of genes involved in thiol homeostasis, during periods of extreme stress By similarity. HAMAP MF_01132
Subcellular location	Cytoplasm Probable HAMAP MF_01132.
Sequence similarities	Belongs to the ArsC family. Spx subfamily.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm
Domain	Redox-active center
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 131	131	Regulatory protein spx HAMAP MF_01132		PRO_0000162557
Amino acid modifications
	Disulfide bond	10 ↔ 13		Redox-active Potential

Sequences

	Sequence		Length	Mass (Da)
	P60377 [UniParc]. Last modified February 16, 2004. Version 1. Checksum: 67181E1BA6CD9444	FASTA	131	15,582
	10 20 30 40 50 60 MVTLYTSPSC TSCRKARAWL EEHDIPYKER NIFSEPLSLD EIKEILRMTE DGTDEIISTR 70 80 90 100 110 120 SKTFQKLNVD LDSLPLQQLF ELIQKNPGLL RRPIIIDEKR LQVGYNEDEI RRFLPRRVRT 130 YQLREAQKMV N

References

[1]

"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed: 11679669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIP 11262 / Serovar 6a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL596171 Genomic DNA. Translation: CAC97523.1.
PIR	AC1719.
RefSeq	NP_471627.1. NC_003212.1.
3D structure databases
ProteinModelPortal	P60377.
SMR	P60377. Positions 1-118.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1131044.
GenomeReviews	Gene locus lin2295 in contig AL592022_GR.
KEGG	lin:lin2295.
PATRIC	20301425. VBILisInn102668_2345.
Organism-specific databases
GenoList	LIN2295.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734370.
OMA	FTERNIF.
ProtClustDB	PRK01655.
Enzyme and pathway databases
BioCyc	LINN272626:LIN2295-MONOMER.
Family and domain databases
HAMAP	MF_01132. SpxA. [Tree]
InterPro	IPR006660. Arsenate_reductase-like. IPR012336. Thioredoxin-like_fold. IPR023731. Tscrpt_reg_Spx. IPR006504. Tscrpt_reg_Spx/MgsR. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
KO	K00537.
Pfam	PF03960. ArsC. 1 hit. [Graphical view]
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
TIGRFAMs	TIGR01617. ArsC_related. 1 hit.
PROSITE	PS51353. ARSC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPX_LISIN

Accession

Primary (citable) accession number: P60377
Secondary accession number(s): Q929I0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	February 16, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program