P60375 (SPX2_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Regulatory protein spx 2

Alternative name(s):
Thermoresistant insertional mutant protein A

Gene names

Name:	spxA2
Synonyms:	trmA
Ordered Locus Names:	LL0675
ORF Names:	L73853

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	132 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Interferes with activator-stimulated transcription by interaction with the RNA polymerase alpha-CTD. May function to globally reduce transcription of genes involved in growth- and development-promoting processes and to increase transcription of genes involved in thiol homeostasis, during periods of extreme stress By similarity. HAMAP MF_01132
Subcellular location	Cytoplasm Probable HAMAP MF_01132.
Sequence similarities	Belongs to the ArsC family. Spx subfamily.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm
Domain	Redox-active center
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 132	132	Regulatory protein spx 2 HAMAP MF_01132		PRO_0000162554
Amino acid modifications
	Disulfide bond	10 ↔ 13		Redox-active Potential

Sequences

	Sequence		Length	Mass (Da)
	P60375 [UniParc]. Last modified February 16, 2004. Version 1. Checksum: F63EE4B8204136B3	FASTA	132	15,030
	10 20 30 40 50 60 MITIYTAPSC TSCKKAKTWL SYHHIPFNER NLIADPLSTT EISQILQKCD DGVEGLISSR 70 80 90 100 110 120 NRFVKTLGVD FEDISLSQAI KIISENPQIM RRPIIMDEKR LHVGYNEEEI RAFLPRTVRV 130 LENGGARLRS AI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.
[2]	"Effects of metabolic flux on stress response pathways in Lactococcus lactis." Duwat P., Ehrlich S.D., Gruss A. Mol. Microbiol. 31:845-858(1999) [PubMed: 10048028] [Abstract] Cited for: INVOLVEMENT IN STRESS RESPONSE.
[3]	"Inactivation of a gene that is highly conserved in Gram-positive bacteria stimulates degradation of non-native proteins and concomitantly increases stress tolerance in Lactococcus lactis." Frees D., Varmanen P., Ingmer H. Mol. Microbiol. 41:93-103(2001) [PubMed: 11454203] [Abstract] Cited for: INVOLVEMENT IN STRESS TOLERANCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005176 Genomic DNA. Translation: AAK04773.1.
PIR	C86709.
RefSeq	NP_266831.1. NC_002662.1.
3D structure databases
ProteinModelPortal	P60375.
SMR	P60375. Positions 1-118.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1114299.
GenomeReviews	Gene locus LL0675 in contig AE005176_GR.
KEGG	lla:L73853.
NMPDR	fig|272623.1.peg.693.
PATRIC	22293630. VBILacLac136773_0722.
Phylogenomic databases
HOGENOM	HBG734370.
OMA	HERNMNA.
ProtClustDB	PRK01655.
Enzyme and pathway databases
BioCyc	LLAC272623:L73853-MONOMER.
Family and domain databases
HAMAP	MF_01132. SpxA. Divergent sequence. [Tree]
InterPro	IPR006660. Arsenate_reductase-like. IPR012336. Thioredoxin-like_fold. IPR006504. Tscrpt_reg_Spx/MgsR. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF03960. ArsC. 1 hit. [Graphical view]
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
TIGRFAMs	TIGR01617. ArsC_related. 1 hit.
PROSITE	PS51353. ARSC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPX2_LACLA

Accession

Primary (citable) accession number: P60375
Secondary accession number(s): Q9ETA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	February 16, 2004
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program