Reviewed, UniProtKB/Swiss-Prot P60375 (SPX2_LACLA)

Names and origin

Protein names

Recommended name:
    Regulatory protein spx 2
Alternative name(s):
    Thermoresistant insertional mutant protein A

Gene names

Name:	spxA2
Synonyms:	trmA
Ordered Locus Names:	LL0675
ORF Names:	L73853

Organism

Lactococcus lactis subsp. lactis (Streptococcus lactis) [Complete proteome] [HAMAP]

Taxonomic identifier

1360 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	132 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interferes with activator-stimulated transcription by interaction with the RNA polymerase alpha-CTD. May function to globally reduce transcription of genes involved in growth- and development-promoting processes and to increase transcription of genes involved in thiol homeostasis, during periods of extreme stress By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the arsC family. Spx subfamily.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm
Domain	Redox-active center
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transcription regulator activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 132	132	Regulatory protein spx 2 HAMAP MF_01132		PRO_0000162554
Amino acid modifications
	Disulfide bond	10 ↔ 13		Redox-active Potential

Sequences

	Sequence		Length	Mass (Da)
	P60375-1 [UniParc]. Last modified February 16, 2004. Version 1. Checksum: F63EE4B8204136B3	FASTA	132	15,030
	10 20 30 40 50 60 MITIYTAPSC TSCKKAKTWL SYHHIPFNER NLIADPLSTT EISQILQKCD DGVEGLISSR 70 80 90 100 110 120 NRFVKTLGVD FEDISLSQAI KIISENPQIM RRPIIMDEKR LHVGYNEEEI RAFLPRTVRV 130 LENGGARLRS AI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.
[2]	"Effects of metabolic flux on stress response pathways in Lactococcus lactis." Duwat P., Ehrlich S.D., Gruss A. Mol. Microbiol. 31:845-858(1999) [PubMed: 10048028] [Abstract] Cited for: INVOLVEMENT IN STRESS RESPONSE.
[3]	"Inactivation of a gene that is highly conserved in Gram-positive bacteria stimulates degradation of non-native proteins and concomitantly increases stress tolerance in Lactococcus lactis." Frees D., Varmanen P., Ingmer H. Mol. Microbiol. 41:93-103(2001) [PubMed: 11454203] [Abstract] Cited for: INVOLVEMENT IN STRESS TOLERANCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006301 Genomic DNA. Translation: AAK04773.1.
PIR	C86709.
RefSeq	NP_266831.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1114299.
GenomeReviews	Gene locus LL0675 in contig AE005176_GR.
KEGG	lla:L73853.
NMPDR	fig|272623.1.peg.693.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P60375.
OMA	P60375. DIIMETP.
Enzyme and pathway databases
BioCyc	LLAC272623:L73853-MON.
Family and domain databases
HAMAP	MF_01132. Divergent sequence. [Tree]
InterPro	IPR006660. Arsenate_reductase-like. IPR006504. CHP1617_ArsC_rel. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF03960. ArsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR01617. arsC_related. 1 hit.
PROSITE	PS51353. ARSC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPX2_LACLA

Accession

Primary (citable) accession number: P60375
Secondary accession number(s): Q9ETA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	February 16, 2004
Last modified:	June 16, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)